Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_473 and Pf6N2E2_474 are separated by 82 nucleotides Pf6N2E2_474 and Pf6N2E2_475 are separated by 76 nucleotides Pf6N2E2_475 and Pf6N2E2_476 are separated by 110 nucleotides
Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272
_473
Pf6N2E2_474 - Transcriptional regulator, ArsR family, at 539,355 to 540,110
_474
Pf6N2E2_475 - Transcriptional regulator, AraC family, at 540,187 to 540,981
_475
Pf6N2E2_476 - Permease of the drug/metabolite transporter (DMT) superfamily, at 541,092 to 542,021
_476
Position (kb)
540
541 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 539.227 kb on - strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.310 kb on - strand at 539.310 kb on - strand at 539.312 kb on + strand at 539.577 kb on + strand, within Pf6N2E2_474 at 539.577 kb on + strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.697 kb on + strand, within Pf6N2E2_474 at 539.705 kb on + strand, within Pf6N2E2_474 at 539.706 kb on - strand, within Pf6N2E2_474 at 540.198 kb on + strand at 540.198 kb on + strand at 540.710 kb on + strand, within Pf6N2E2_475 at 540.711 kb on - strand, within Pf6N2E2_475 at 540.711 kb on - strand, within Pf6N2E2_475 at 540.800 kb on + strand, within Pf6N2E2_475 at 540.801 kb on - strand, within Pf6N2E2_475 at 540.801 kb on - strand, within Pf6N2E2_475 at 540.839 kb on + strand, within Pf6N2E2_475 at 540.839 kb on + strand, within Pf6N2E2_475 at 540.839 kb on + strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.845 kb on + strand, within Pf6N2E2_475 at 540.882 kb on - strand, within Pf6N2E2_475 at 540.981 kb on + strand at 541.028 kb on + strand at 541.028 kb on + strand at 541.029 kb on - strand at 541.029 kb on - strand at 541.029 kb on - strand at 541.029 kb on - strand at 541.051 kb on - strand at 541.373 kb on + strand, within Pf6N2E2_476 at 541.373 kb on + strand, within Pf6N2E2_476 at 541.405 kb on - strand, within Pf6N2E2_476 at 541.615 kb on + strand, within Pf6N2E2_476 at 541.616 kb on - strand, within Pf6N2E2_476 at 541.919 kb on + strand, within Pf6N2E2_476
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 539,227 - +1.0 539,309 + -0.8 539,309 + -0.6 539,309 + -2.4 539,309 + -2.2 539,309 + -2.4 539,309 + -0.5 539,309 + +0.5 539,310 - +0.7 539,310 - -0.8 539,312 + +0.2 539,577 + Pf6N2E2_474 0.29 -1.1 539,577 + Pf6N2E2_474 0.29 -0.1 539,578 - Pf6N2E2_474 0.29 +0.3 539,578 - Pf6N2E2_474 0.29 +0.7 539,578 - Pf6N2E2_474 0.29 +0.2 539,697 + Pf6N2E2_474 0.45 +0.1 539,705 + Pf6N2E2_474 0.46 -0.4 539,706 - Pf6N2E2_474 0.46 -0.3 540,198 + +1.2 540,198 + -1.3 540,710 + Pf6N2E2_475 0.66 -0.6 540,711 - Pf6N2E2_475 0.66 +0.3 540,711 - Pf6N2E2_475 0.66 -0.6 540,800 + Pf6N2E2_475 0.77 -0.4 540,801 - Pf6N2E2_475 0.77 -0.3 540,801 - Pf6N2E2_475 0.77 -2.0 540,839 + Pf6N2E2_475 0.82 -1.0 540,839 + Pf6N2E2_475 0.82 +0.2 540,839 + Pf6N2E2_475 0.82 -3.2 540,840 - Pf6N2E2_475 0.82 +1.7 540,840 - Pf6N2E2_475 0.82 -0.2 540,840 - Pf6N2E2_475 0.82 -0.3 540,840 - Pf6N2E2_475 0.82 -0.2 540,840 - Pf6N2E2_475 0.82 -1.8 540,845 + Pf6N2E2_475 0.83 -0.5 540,882 - Pf6N2E2_475 0.87 +1.7 540,981 + +2.9 541,028 + +1.7 541,028 + -1.5 541,029 - -0.5 541,029 - -1.5 541,029 - +1.7 541,029 - +1.5 541,051 - -1.4 541,373 + Pf6N2E2_476 0.30 -2.5 541,373 + Pf6N2E2_476 0.30 -0.8 541,405 - Pf6N2E2_476 0.34 +1.7 541,615 + Pf6N2E2_476 0.56 +0.2 541,616 - Pf6N2E2_476 0.56 -0.3 541,919 + Pf6N2E2_476 0.89 -3.2
Or see this region's nucleotide sequence