Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_327 and Pf6N2E2_328 are separated by 246 nucleotides Pf6N2E2_328 and Pf6N2E2_329 are separated by 161 nucleotides Pf6N2E2_329 and Pf6N2E2_330 are separated by 241 nucleotides
Pf6N2E2_327 - Alginate lyase precursor (EC 4.2.2.3), at 395,293 to 395,949
_327
Pf6N2E2_328 - hypothetical protein, at 396,196 to 396,531
_328
Pf6N2E2_329 - L-lactate permease, at 396,693 to 398,456
_329
Pf6N2E2_330 - Mesenchymal stem cell protein DSCD75, at 398,698 to 399,249
_330
Position (kb) 396
397
398
399 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 395.855 kb on + strand, within Pf6N2E2_327 at 395.855 kb on + strand, within Pf6N2E2_327 at 395.856 kb on - strand, within Pf6N2E2_327 at 395.856 kb on - strand, within Pf6N2E2_327 at 395.952 kb on + strand at 395.952 kb on + strand at 395.952 kb on + strand at 395.952 kb on + strand at 396.112 kb on + strand at 396.146 kb on + strand at 396.146 kb on + strand at 396.146 kb on + strand at 396.283 kb on + strand, within Pf6N2E2_328 at 396.283 kb on + strand, within Pf6N2E2_328 at 396.283 kb on + strand, within Pf6N2E2_328 at 396.284 kb on - strand, within Pf6N2E2_328 at 396.298 kb on + strand, within Pf6N2E2_328 at 396.298 kb on + strand, within Pf6N2E2_328 at 396.482 kb on - strand, within Pf6N2E2_328 at 396.547 kb on + strand at 396.547 kb on + strand at 396.547 kb on + strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.565 kb on - strand at 396.574 kb on + strand at 396.574 kb on + strand at 396.713 kb on + strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.733 kb on + strand at 396.733 kb on + strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 397.126 kb on - strand, within Pf6N2E2_329 at 397.126 kb on - strand, within Pf6N2E2_329 at 397.126 kb on - strand, within Pf6N2E2_329 at 397.134 kb on - strand, within Pf6N2E2_329 at 397.134 kb on - strand, within Pf6N2E2_329 at 397.171 kb on + strand, within Pf6N2E2_329 at 397.172 kb on - strand, within Pf6N2E2_329 at 397.199 kb on - strand, within Pf6N2E2_329 at 397.199 kb on - strand, within Pf6N2E2_329 at 397.274 kb on - strand, within Pf6N2E2_329 at 397.292 kb on + strand, within Pf6N2E2_329 at 397.729 kb on + strand, within Pf6N2E2_329 at 397.729 kb on + strand, within Pf6N2E2_329 at 397.730 kb on - strand, within Pf6N2E2_329 at 398.248 kb on + strand, within Pf6N2E2_329 at 398.249 kb on - strand, within Pf6N2E2_329 at 398.437 kb on + strand at 398.438 kb on - strand at 398.474 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.517 kb on - strand at 398.517 kb on - strand at 398.517 kb on - strand at 398.518 kb on + strand at 398.519 kb on - strand at 398.623 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.684 kb on - strand at 398.684 kb on - strand at 399.074 kb on + strand, within Pf6N2E2_330 at 399.074 kb on + strand, within Pf6N2E2_330 at 399.075 kb on - strand, within Pf6N2E2_330 at 399.107 kb on + strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.283 kb on + strand at 399.284 kb on - strand at 399.307 kb on - strand at 399.392 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 395,855 + Pf6N2E2_327 0.86 +0.7 395,855 + Pf6N2E2_327 0.86 +1.7 395,856 - Pf6N2E2_327 0.86 -0.4 395,856 - Pf6N2E2_327 0.86 -2.3 395,952 + -2.5 395,952 + -1.7 395,952 + -1.0 395,952 + -0.4 396,112 + -0.2 396,146 + -0.6 396,146 + +0.6 396,146 + +0.1 396,283 + Pf6N2E2_328 0.26 +0.5 396,283 + Pf6N2E2_328 0.26 +0.5 396,283 + Pf6N2E2_328 0.26 +1.9 396,284 - Pf6N2E2_328 0.26 +1.7 396,298 + Pf6N2E2_328 0.30 +1.7 396,298 + Pf6N2E2_328 0.30 +1.5 396,482 - Pf6N2E2_328 0.85 -0.6 396,547 + -0.3 396,547 + -0.9 396,547 + +0.3 396,548 - +0.8 396,548 - -0.8 396,548 - +0.1 396,548 - -0.9 396,548 - -0.1 396,565 - +0.3 396,574 + +0.2 396,574 + +1.4 396,713 + +0.1 396,714 - -3.6 396,714 - +1.1 396,714 - -0.9 396,714 - +0.1 396,714 - -1.9 396,714 - +0.1 396,714 - +0.1 396,733 + +1.1 396,733 + -3.1 396,734 - +0.4 396,734 - -0.5 396,734 - +0.3 396,734 - -1.1 396,734 - +1.7 396,734 - +0.7 397,126 - Pf6N2E2_329 0.25 -0.2 397,126 - Pf6N2E2_329 0.25 -1.1 397,126 - Pf6N2E2_329 0.25 -0.8 397,134 - Pf6N2E2_329 0.25 -0.8 397,134 - Pf6N2E2_329 0.25 -1.6 397,171 + Pf6N2E2_329 0.27 -2.6 397,172 - Pf6N2E2_329 0.27 -0.3 397,199 - Pf6N2E2_329 0.29 -0.8 397,199 - Pf6N2E2_329 0.29 -2.3 397,274 - Pf6N2E2_329 0.33 +0.1 397,292 + Pf6N2E2_329 0.34 +0.9 397,729 + Pf6N2E2_329 0.59 -1.2 397,729 + Pf6N2E2_329 0.59 +1.7 397,730 - Pf6N2E2_329 0.59 +1.7 398,248 + Pf6N2E2_329 0.88 +1.7 398,249 - Pf6N2E2_329 0.88 -0.6 398,437 + +0.8 398,438 - +0.7 398,474 + +1.7 398,516 + -0.3 398,516 + +1.7 398,516 + -1.5 398,516 + -1.1 398,516 + -0.2 398,517 - +1.7 398,517 - +1.5 398,517 - -0.1 398,518 + -0.8 398,519 - +0.3 398,623 + -1.0 398,683 + -1.0 398,683 + +0.2 398,683 + -0.3 398,683 + -0.4 398,684 - -0.3 398,684 - -2.3 399,074 + Pf6N2E2_330 0.68 +0.1 399,074 + Pf6N2E2_330 0.68 +0.1 399,075 - Pf6N2E2_330 0.68 +0.3 399,107 + Pf6N2E2_330 0.74 -0.1 399,108 - Pf6N2E2_330 0.74 -0.4 399,108 - Pf6N2E2_330 0.74 -1.3 399,283 + -0.3 399,284 - -0.5 399,307 - -0.1 399,392 + -0.3
Or see this region's nucleotide sequence