Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2882 and Pf6N2E2_2883 are separated by 233 nucleotides Pf6N2E2_2883 and Pf6N2E2_2884 are separated by 17 nucleotides Pf6N2E2_2884 and Pf6N2E2_2885 overlap by 14 nucleotides
Pf6N2E2_2882 - Isopenicillin N epimerase (EC 5.1.1.17), at 3,369,514 to 3,370,701
_2882
Pf6N2E2_2883 - 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 3,370,935 to 3,371,600
_2883
Pf6N2E2_2884 - 6-phosphogluconolactonase (EC 3.1.1.31), eukaryotic type, at 3,371,618 to 3,372,331
_2884
Pf6N2E2_2885 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 3,372,318 to 3,373,787
_2885
Position (kb)
3370
3371
3372 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.037 kb on - strand, within Pf6N2E2_2882 at 3370.037 kb on - strand, within Pf6N2E2_2882 at 3370.235 kb on - strand, within Pf6N2E2_2882 at 3370.235 kb on - strand, within Pf6N2E2_2882 at 3370.288 kb on + strand, within Pf6N2E2_2882 at 3370.289 kb on - strand, within Pf6N2E2_2882 at 3370.289 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.498 kb on + strand, within Pf6N2E2_2882 at 3370.498 kb on + strand, within Pf6N2E2_2882 at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.852 kb on + strand at 3370.852 kb on + strand at 3370.853 kb on - strand at 3370.853 kb on - strand at 3370.898 kb on - strand at 3370.905 kb on + strand at 3370.906 kb on - strand at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.645 kb on - strand at 3371.645 kb on - strand at 3372.332 kb on - strand at 3372.533 kb on - strand, within Pf6N2E2_2885 at 3372.560 kb on - strand, within Pf6N2E2_2885
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 3,370,036 + Pf6N2E2_2882 0.44 -0.9 3,370,036 + Pf6N2E2_2882 0.44 -0.5 3,370,036 + Pf6N2E2_2882 0.44 -0.3 3,370,036 + Pf6N2E2_2882 0.44 -0.2 3,370,037 - Pf6N2E2_2882 0.44 -0.6 3,370,037 - Pf6N2E2_2882 0.44 -1.7 3,370,235 - Pf6N2E2_2882 0.61 +0.2 3,370,235 - Pf6N2E2_2882 0.61 -1.0 3,370,288 + Pf6N2E2_2882 0.65 -3.2 3,370,289 - Pf6N2E2_2882 0.65 -0.7 3,370,289 - Pf6N2E2_2882 0.65 +1.3 3,370,421 - Pf6N2E2_2882 0.76 -0.3 3,370,421 - Pf6N2E2_2882 0.76 -3.0 3,370,421 - Pf6N2E2_2882 0.76 +0.7 3,370,498 + Pf6N2E2_2882 0.83 -0.5 3,370,498 + Pf6N2E2_2882 0.83 -0.2 3,370,699 + -0.2 3,370,699 + +1.4 3,370,699 + -0.6 3,370,699 + -1.5 3,370,699 + -1.9 3,370,699 + -0.9 3,370,699 + -2.4 3,370,699 + +0.9 3,370,699 + +0.7 3,370,699 + +1.7 3,370,700 - -0.4 3,370,700 - -0.8 3,370,700 - -1.0 3,370,700 - -0.2 3,370,700 - +1.7 3,370,700 - +1.1 3,370,700 - -0.7 3,370,700 - +0.1 3,370,700 - -0.1 3,370,700 - +0.7 3,370,700 - +0.3 3,370,700 - -0.3 3,370,700 - +1.7 3,370,700 - +0.4 3,370,852 + -0.7 3,370,852 + -0.8 3,370,853 - -0.6 3,370,853 - +1.7 3,370,898 - -0.9 3,370,905 + -3.2 3,370,906 - +0.3 3,371,632 - -1.5 3,371,632 - -1.4 3,371,632 - -1.8 3,371,645 - -0.2 3,371,645 - +0.3 3,372,332 - -0.1 3,372,533 - Pf6N2E2_2885 0.15 -0.7 3,372,560 - Pf6N2E2_2885 0.16 -0.9
Or see this region's nucleotide sequence