Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_255 and Pf6N2E2_256 overlap by 8 nucleotides Pf6N2E2_256 and Pf6N2E2_257 are separated by 118 nucleotides Pf6N2E2_257 and Pf6N2E2_258 are separated by 145 nucleotides
Pf6N2E2_255 - DoxX family protein, at 319,191 to 319,604
_255
Pf6N2E2_256 - putative membrane protein, at 319,597 to 320,235
_256
Pf6N2E2_257 - Exoenzymes regulatory protein AepA precursor, at 320,354 to 322,192
_257
Pf6N2E2_258 - Nicotinamidase family protein YcaC, at 322,338 to 322,967
_258
Position (kb) 320
321
322
323 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.733 kb on + strand, within Pf6N2E2_256 at 319.734 kb on - strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.143 kb on + strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.333 kb on - strand at 320.393 kb on - strand at 320.790 kb on - strand, within Pf6N2E2_257 at 320.834 kb on + strand, within Pf6N2E2_257 at 320.975 kb on + strand, within Pf6N2E2_257 at 321.077 kb on + strand, within Pf6N2E2_257 at 321.078 kb on - strand, within Pf6N2E2_257 at 321.131 kb on + strand, within Pf6N2E2_257 at 321.132 kb on - strand, within Pf6N2E2_257 at 321.383 kb on + strand, within Pf6N2E2_257 at 321.383 kb on + strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.579 kb on - strand, within Pf6N2E2_257 at 321.579 kb on - strand, within Pf6N2E2_257 at 321.677 kb on + strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.699 kb on - strand, within Pf6N2E2_257 at 321.947 kb on + strand, within Pf6N2E2_257 at 321.948 kb on - strand, within Pf6N2E2_257 at 322.311 kb on + strand at 322.312 kb on - strand at 322.312 kb on - strand at 322.356 kb on + strand at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.767 kb on - strand, within Pf6N2E2_258 at 322.944 kb on + strand at 322.944 kb on + strand at 322.945 kb on - strand at 322.945 kb on - strand at 322.965 kb on + strand at 322.966 kb on - strand at 322.966 kb on - strand at 322.966 kb on - strand at 322.966 kb on - strand at 323.026 kb on + strand at 323.027 kb on - strand at 323.027 kb on - strand at 323.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 319,525 + Pf6N2E2_255 0.81 +1.7 319,525 + Pf6N2E2_255 0.81 -0.3 319,526 - Pf6N2E2_255 0.81 +1.7 319,526 - Pf6N2E2_255 0.81 -1.9 319,537 + Pf6N2E2_255 0.84 -2.9 319,537 + Pf6N2E2_255 0.84 +0.1 319,538 - Pf6N2E2_255 0.84 +1.7 319,538 - Pf6N2E2_255 0.84 -1.0 319,733 + Pf6N2E2_256 0.21 +0.1 319,734 - Pf6N2E2_256 0.21 +0.7 320,092 + Pf6N2E2_256 0.77 +1.9 320,092 + Pf6N2E2_256 0.77 -2.7 320,092 + Pf6N2E2_256 0.77 +0.3 320,092 + Pf6N2E2_256 0.77 +0.7 320,093 - Pf6N2E2_256 0.78 -0.3 320,093 - Pf6N2E2_256 0.78 -3.4 320,093 - Pf6N2E2_256 0.78 +1.0 320,093 - Pf6N2E2_256 0.78 +0.1 320,143 + Pf6N2E2_256 0.85 -0.1 320,144 - Pf6N2E2_256 0.86 -1.1 320,144 - Pf6N2E2_256 0.86 -0.1 320,144 - Pf6N2E2_256 0.86 +0.1 320,144 - Pf6N2E2_256 0.86 +0.3 320,144 - Pf6N2E2_256 0.86 +0.0 320,333 - -1.6 320,393 - -3.0 320,790 - Pf6N2E2_257 0.24 +0.1 320,834 + Pf6N2E2_257 0.26 +0.0 320,975 + Pf6N2E2_257 0.34 -1.1 321,077 + Pf6N2E2_257 0.39 -0.3 321,078 - Pf6N2E2_257 0.39 +0.6 321,131 + Pf6N2E2_257 0.42 -2.0 321,132 - Pf6N2E2_257 0.42 -2.3 321,383 + Pf6N2E2_257 0.56 -0.7 321,383 + Pf6N2E2_257 0.56 -1.0 321,384 - Pf6N2E2_257 0.56 -2.1 321,384 - Pf6N2E2_257 0.56 +0.1 321,384 - Pf6N2E2_257 0.56 -1.9 321,384 - Pf6N2E2_257 0.56 -2.7 321,384 - Pf6N2E2_257 0.56 -0.0 321,384 - Pf6N2E2_257 0.56 -0.4 321,579 - Pf6N2E2_257 0.67 -0.5 321,579 - Pf6N2E2_257 0.67 +0.1 321,677 + Pf6N2E2_257 0.72 +0.1 321,678 - Pf6N2E2_257 0.72 -0.8 321,678 - Pf6N2E2_257 0.72 +0.1 321,678 - Pf6N2E2_257 0.72 -0.6 321,699 - Pf6N2E2_257 0.73 +0.1 321,947 + Pf6N2E2_257 0.87 -1.0 321,948 - Pf6N2E2_257 0.87 -0.5 322,311 + +1.7 322,312 - +0.4 322,312 - +0.0 322,356 + +1.7 322,584 + Pf6N2E2_258 0.39 +0.3 322,584 + Pf6N2E2_258 0.39 -0.5 322,584 + Pf6N2E2_258 0.39 -1.0 322,584 + Pf6N2E2_258 0.39 -1.3 322,584 + Pf6N2E2_258 0.39 +0.1 322,584 + Pf6N2E2_258 0.39 +0.9 322,767 - Pf6N2E2_258 0.68 +0.1 322,944 + -0.6 322,944 + +1.0 322,945 - -1.5 322,945 - -0.7 322,965 + +0.7 322,966 - -1.1 322,966 - -0.8 322,966 - -0.7 322,966 - +1.7 323,026 + -2.7 323,027 - -0.5 323,027 - -0.1 323,047 + +0.1
Or see this region's nucleotide sequence