Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2024 and Pf6N2E2_2025 are separated by 613 nucleotides Pf6N2E2_2025 and Pf6N2E2_2026 are separated by 65 nucleotides Pf6N2E2_2026 and Pf6N2E2_2027 are separated by 45 nucleotides
Pf6N2E2_2024 - Hydrolase, carbon-nitrogen family, at 2,412,607 to 2,413,368
_2024
Pf6N2E2_2025 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 2,413,982 to 2,415,052
_2025
Pf6N2E2_2026 - hypothetical protein, at 2,415,118 to 2,415,924
_2026
Pf6N2E2_2027 - hypothetical protein, at 2,415,970 to 2,416,140
_2027
Position (kb)
2413
2414
2415
2416 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2413.038 kb on - strand, within Pf6N2E2_2024 at 2413.038 kb on - strand, within Pf6N2E2_2024 at 2413.246 kb on + strand, within Pf6N2E2_2024 at 2413.409 kb on + strand at 2413.410 kb on - strand at 2413.490 kb on + strand at 2413.490 kb on + strand at 2413.493 kb on + strand at 2413.493 kb on + strand at 2413.493 kb on + strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.617 kb on + strand at 2413.790 kb on + strand at 2413.790 kb on + strand at 2413.791 kb on - strand at 2413.791 kb on - strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.835 kb on - strand at 2413.879 kb on + strand at 2413.934 kb on + strand at 2413.934 kb on + strand at 2413.934 kb on + strand at 2413.935 kb on - strand at 2414.219 kb on + strand, within Pf6N2E2_2025 at 2414.286 kb on - strand, within Pf6N2E2_2025 at 2414.286 kb on - strand, within Pf6N2E2_2025 at 2414.330 kb on + strand, within Pf6N2E2_2025 at 2414.459 kb on + strand, within Pf6N2E2_2025 at 2414.460 kb on - strand, within Pf6N2E2_2025 at 2414.729 kb on + strand, within Pf6N2E2_2025 at 2414.730 kb on - strand, within Pf6N2E2_2025 at 2414.730 kb on - strand, within Pf6N2E2_2025 at 2414.730 kb on - strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.755 kb on - strand, within Pf6N2E2_2025 at 2414.755 kb on - strand, within Pf6N2E2_2025 at 2414.755 kb on - strand, within Pf6N2E2_2025 at 2415.198 kb on - strand at 2415.198 kb on - strand at 2415.198 kb on - strand at 2415.236 kb on + strand, within Pf6N2E2_2026 at 2415.236 kb on + strand, within Pf6N2E2_2026 at 2415.236 kb on + strand, within Pf6N2E2_2026 at 2415.237 kb on - strand, within Pf6N2E2_2026 at 2415.237 kb on - strand, within Pf6N2E2_2026 at 2415.305 kb on + strand, within Pf6N2E2_2026 at 2415.305 kb on + strand, within Pf6N2E2_2026 at 2415.305 kb on + strand, within Pf6N2E2_2026 at 2415.306 kb on - strand, within Pf6N2E2_2026 at 2415.306 kb on - strand, within Pf6N2E2_2026 at 2415.435 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.548 kb on - strand, within Pf6N2E2_2026 at 2415.548 kb on - strand, within Pf6N2E2_2026 at 2415.548 kb on - strand, within Pf6N2E2_2026 at 2415.613 kb on + strand, within Pf6N2E2_2026 at 2415.613 kb on + strand, within Pf6N2E2_2026 at 2415.613 kb on + strand, within Pf6N2E2_2026 at 2415.614 kb on - strand, within Pf6N2E2_2026 at 2415.793 kb on + strand, within Pf6N2E2_2026 at 2415.794 kb on - strand, within Pf6N2E2_2026 at 2415.850 kb on - strand at 2415.938 kb on - strand at 2415.977 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 2,413,038 - Pf6N2E2_2024 0.57 -2.5 2,413,038 - Pf6N2E2_2024 0.57 +0.1 2,413,246 + Pf6N2E2_2024 0.84 -0.8 2,413,409 + -1.0 2,413,410 - +1.3 2,413,490 + -1.4 2,413,490 + -0.4 2,413,493 + -1.2 2,413,493 + +1.9 2,413,493 + +0.5 2,413,494 - -2.8 2,413,494 - +1.7 2,413,494 - -0.8 2,413,494 - +0.8 2,413,494 - -2.2 2,413,617 + -0.1 2,413,790 + +0.9 2,413,790 + +0.7 2,413,791 - -0.4 2,413,791 - -0.3 2,413,799 + +1.5 2,413,799 + -0.1 2,413,799 + +1.7 2,413,799 + +0.2 2,413,799 + +0.1 2,413,800 - +1.7 2,413,800 - -0.6 2,413,800 - -1.4 2,413,800 - -0.6 2,413,800 - -0.3 2,413,800 - +0.2 2,413,800 - -0.9 2,413,835 - +0.6 2,413,879 + -0.6 2,413,934 + -0.5 2,413,934 + +0.1 2,413,934 + -2.0 2,413,935 - +0.1 2,414,219 + Pf6N2E2_2025 0.22 -0.9 2,414,286 - Pf6N2E2_2025 0.28 -0.2 2,414,286 - Pf6N2E2_2025 0.28 +0.3 2,414,330 + Pf6N2E2_2025 0.32 -1.3 2,414,459 + Pf6N2E2_2025 0.45 +1.7 2,414,460 - Pf6N2E2_2025 0.45 -0.6 2,414,729 + Pf6N2E2_2025 0.70 -3.1 2,414,730 - Pf6N2E2_2025 0.70 -0.5 2,414,730 - Pf6N2E2_2025 0.70 -1.6 2,414,730 - Pf6N2E2_2025 0.70 +1.7 2,414,754 + Pf6N2E2_2025 0.72 +0.7 2,414,754 + Pf6N2E2_2025 0.72 -0.6 2,414,754 + Pf6N2E2_2025 0.72 -2.6 2,414,754 + Pf6N2E2_2025 0.72 -1.3 2,414,754 + Pf6N2E2_2025 0.72 +0.2 2,414,755 - Pf6N2E2_2025 0.72 -0.5 2,414,755 - Pf6N2E2_2025 0.72 +0.5 2,414,755 - Pf6N2E2_2025 0.72 +0.7 2,415,198 - +0.2 2,415,198 - -0.7 2,415,198 - +0.2 2,415,236 + Pf6N2E2_2026 0.15 +4.1 2,415,236 + Pf6N2E2_2026 0.15 +0.7 2,415,236 + Pf6N2E2_2026 0.15 +1.7 2,415,237 - Pf6N2E2_2026 0.15 -1.4 2,415,237 - Pf6N2E2_2026 0.15 -0.6 2,415,305 + Pf6N2E2_2026 0.23 -0.8 2,415,305 + Pf6N2E2_2026 0.23 -0.6 2,415,305 + Pf6N2E2_2026 0.23 -0.4 2,415,306 - Pf6N2E2_2026 0.23 +0.5 2,415,306 - Pf6N2E2_2026 0.23 -0.4 2,415,435 + Pf6N2E2_2026 0.39 +0.3 2,415,524 + Pf6N2E2_2026 0.50 +0.5 2,415,524 + Pf6N2E2_2026 0.50 -1.7 2,415,524 + Pf6N2E2_2026 0.50 -2.2 2,415,524 + Pf6N2E2_2026 0.50 +0.4 2,415,525 - Pf6N2E2_2026 0.50 +0.6 2,415,525 - Pf6N2E2_2026 0.50 -0.1 2,415,525 - Pf6N2E2_2026 0.50 +1.7 2,415,525 - Pf6N2E2_2026 0.50 +1.7 2,415,547 + Pf6N2E2_2026 0.53 -0.2 2,415,547 + Pf6N2E2_2026 0.53 +1.5 2,415,547 + Pf6N2E2_2026 0.53 -1.2 2,415,547 + Pf6N2E2_2026 0.53 +1.5 2,415,547 + Pf6N2E2_2026 0.53 +0.7 2,415,547 + Pf6N2E2_2026 0.53 +1.7 2,415,548 - Pf6N2E2_2026 0.53 -1.3 2,415,548 - Pf6N2E2_2026 0.53 +0.1 2,415,548 - Pf6N2E2_2026 0.53 -2.0 2,415,613 + Pf6N2E2_2026 0.61 +0.2 2,415,613 + Pf6N2E2_2026 0.61 +0.5 2,415,613 + Pf6N2E2_2026 0.61 -0.2 2,415,614 - Pf6N2E2_2026 0.61 +1.7 2,415,793 + Pf6N2E2_2026 0.84 -1.4 2,415,794 - Pf6N2E2_2026 0.84 -1.0 2,415,850 - +0.1 2,415,938 - +1.0 2,415,977 + +0.7
Or see this region's nucleotide sequence