Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5105

Experiment: nitrogen source nitrite 1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5103 and Pf6N2E2_5104 are separated by 9 nucleotidesPf6N2E2_5104 and Pf6N2E2_5105 are separated by 80 nucleotidesPf6N2E2_5105 and Pf6N2E2_5106 are separated by 170 nucleotidesPf6N2E2_5106 and Pf6N2E2_5107 are separated by 254 nucleotides Pf6N2E2_5103 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 5,884,996 to 5,886,114 _5103 Pf6N2E2_5104 - Conserved uncharacterized protein CreA, at 5,886,124 to 5,886,579 _5104 Pf6N2E2_5105 - hypothetical protein, at 5,886,660 to 5,888,699 _5105 Pf6N2E2_5106 - SSU ribosomal protein S20p, at 5,888,870 to 5,889,148 _5106 Pf6N2E2_5107 - Proposed peptidoglycan lipid II flippase MurJ, at 5,889,403 to 5,890,941 _5107 Position (kb) 5886 5887 5888 5889Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5885.846 kb on + strand, within Pf6N2E2_5103at 5885.846 kb on + strand, within Pf6N2E2_5103at 5886.287 kb on - strand, within Pf6N2E2_5104at 5886.381 kb on + strand, within Pf6N2E2_5104at 5886.381 kb on + strand, within Pf6N2E2_5104at 5886.381 kb on + strand, within Pf6N2E2_5104at 5886.489 kb on + strand, within Pf6N2E2_5104at 5886.489 kb on + strand, within Pf6N2E2_5104at 5886.490 kb on - strand, within Pf6N2E2_5104at 5886.490 kb on - strand, within Pf6N2E2_5104at 5886.500 kb on - strand, within Pf6N2E2_5104at 5886.500 kb on - strand, within Pf6N2E2_5104at 5886.508 kb on + strand, within Pf6N2E2_5104at 5886.508 kb on + strand, within Pf6N2E2_5104at 5886.577 kb on + strandat 5886.578 kb on - strandat 5886.617 kb on + strandat 5886.621 kb on + strandat 5886.622 kb on - strandat 5886.659 kb on + strandat 5886.660 kb on - strandat 5886.660 kb on - strandat 5886.685 kb on + strandat 5886.730 kb on - strandat 5886.760 kb on + strandat 5886.797 kb on + strandat 5886.820 kb on + strandat 5886.820 kb on + strandat 5886.820 kb on + strandat 5886.826 kb on - strandat 5886.826 kb on - strandat 5886.876 kb on + strand, within Pf6N2E2_5105at 5886.876 kb on + strand, within Pf6N2E2_5105at 5886.876 kb on + strand, within Pf6N2E2_5105at 5886.877 kb on - strand, within Pf6N2E2_5105at 5886.906 kb on + strand, within Pf6N2E2_5105at 5886.906 kb on + strand, within Pf6N2E2_5105at 5886.907 kb on - strand, within Pf6N2E2_5105at 5887.120 kb on - strand, within Pf6N2E2_5105at 5887.194 kb on + strand, within Pf6N2E2_5105at 5887.194 kb on + strand, within Pf6N2E2_5105at 5887.261 kb on - strand, within Pf6N2E2_5105at 5887.308 kb on + strand, within Pf6N2E2_5105at 5887.308 kb on + strand, within Pf6N2E2_5105at 5887.308 kb on + strand, within Pf6N2E2_5105at 5887.308 kb on + strand, within Pf6N2E2_5105at 5887.309 kb on - strand, within Pf6N2E2_5105at 5887.309 kb on - strand, within Pf6N2E2_5105at 5887.309 kb on - strand, within Pf6N2E2_5105at 5887.350 kb on + strand, within Pf6N2E2_5105at 5887.392 kb on + strand, within Pf6N2E2_5105at 5887.393 kb on - strand, within Pf6N2E2_5105at 5887.393 kb on - strand, within Pf6N2E2_5105at 5887.393 kb on - strand, within Pf6N2E2_5105at 5887.393 kb on - strand, within Pf6N2E2_5105at 5887.458 kb on + strand, within Pf6N2E2_5105at 5887.458 kb on + strand, within Pf6N2E2_5105at 5887.458 kb on + strand, within Pf6N2E2_5105at 5887.458 kb on + strand, within Pf6N2E2_5105at 5887.459 kb on - strand, within Pf6N2E2_5105at 5887.459 kb on - strand, within Pf6N2E2_5105at 5887.459 kb on - strand, within Pf6N2E2_5105at 5887.459 kb on - strand, within Pf6N2E2_5105at 5887.459 kb on - strand, within Pf6N2E2_5105at 5887.459 kb on - strand, within Pf6N2E2_5105at 5887.459 kb on - strand, within Pf6N2E2_5105at 5887.527 kb on + strand, within Pf6N2E2_5105at 5887.527 kb on + strand, within Pf6N2E2_5105at 5887.527 kb on + strand, within Pf6N2E2_5105at 5887.527 kb on + strand, within Pf6N2E2_5105at 5887.527 kb on + strand, within Pf6N2E2_5105at 5887.527 kb on + strand, within Pf6N2E2_5105at 5887.527 kb on + strand, within Pf6N2E2_5105at 5887.528 kb on - strand, within Pf6N2E2_5105at 5887.528 kb on - strand, within Pf6N2E2_5105at 5887.528 kb on - strand, within Pf6N2E2_5105at 5887.528 kb on - strand, within Pf6N2E2_5105at 5887.528 kb on - strand, within Pf6N2E2_5105at 5887.608 kb on + strand, within Pf6N2E2_5105at 5887.608 kb on + strand, within Pf6N2E2_5105at 5887.608 kb on + strand, within Pf6N2E2_5105at 5887.671 kb on + strand, within Pf6N2E2_5105at 5887.671 kb on + strand, within Pf6N2E2_5105at 5887.671 kb on + strand, within Pf6N2E2_5105at 5887.671 kb on + strand, within Pf6N2E2_5105at 5887.671 kb on + strand, within Pf6N2E2_5105at 5887.671 kb on + strand, within Pf6N2E2_5105at 5887.671 kb on + strand, within Pf6N2E2_5105at 5887.672 kb on - strand, within Pf6N2E2_5105at 5887.672 kb on - strand, within Pf6N2E2_5105at 5887.672 kb on - strand, within Pf6N2E2_5105at 5887.672 kb on - strand, within Pf6N2E2_5105at 5887.672 kb on - strand, within Pf6N2E2_5105at 5887.794 kb on + strand, within Pf6N2E2_5105at 5887.972 kb on - strand, within Pf6N2E2_5105at 5887.995 kb on + strand, within Pf6N2E2_5105at 5888.007 kb on + strand, within Pf6N2E2_5105at 5888.007 kb on + strand, within Pf6N2E2_5105at 5888.008 kb on - strand, within Pf6N2E2_5105at 5888.008 kb on - strand, within Pf6N2E2_5105at 5888.043 kb on + strand, within Pf6N2E2_5105at 5888.043 kb on + strand, within Pf6N2E2_5105at 5888.043 kb on + strand, within Pf6N2E2_5105at 5888.044 kb on - strand, within Pf6N2E2_5105at 5888.075 kb on + strand, within Pf6N2E2_5105at 5888.118 kb on + strand, within Pf6N2E2_5105at 5888.119 kb on - strand, within Pf6N2E2_5105at 5888.119 kb on - strand, within Pf6N2E2_5105at 5888.141 kb on + strand, within Pf6N2E2_5105at 5888.154 kb on + strand, within Pf6N2E2_5105at 5888.154 kb on + strand, within Pf6N2E2_5105at 5888.154 kb on + strand, within Pf6N2E2_5105at 5888.154 kb on + strand, within Pf6N2E2_5105at 5888.154 kb on + strand, within Pf6N2E2_5105at 5888.154 kb on + strand, within Pf6N2E2_5105at 5888.154 kb on + strand, within Pf6N2E2_5105at 5888.155 kb on - strand, within Pf6N2E2_5105at 5888.155 kb on - strand, within Pf6N2E2_5105at 5888.226 kb on + strand, within Pf6N2E2_5105at 5888.226 kb on + strand, within Pf6N2E2_5105at 5888.226 kb on + strand, within Pf6N2E2_5105at 5888.226 kb on + strand, within Pf6N2E2_5105at 5888.226 kb on + strand, within Pf6N2E2_5105at 5888.226 kb on + strand, within Pf6N2E2_5105at 5888.226 kb on + strand, within Pf6N2E2_5105at 5888.227 kb on - strand, within Pf6N2E2_5105at 5888.227 kb on - strand, within Pf6N2E2_5105at 5888.227 kb on - strand, within Pf6N2E2_5105at 5888.227 kb on - strand, within Pf6N2E2_5105at 5888.227 kb on - strand, within Pf6N2E2_5105at 5888.250 kb on + strand, within Pf6N2E2_5105at 5888.250 kb on + strand, within Pf6N2E2_5105at 5888.250 kb on + strand, within Pf6N2E2_5105at 5888.250 kb on + strand, within Pf6N2E2_5105at 5888.250 kb on + strand, within Pf6N2E2_5105at 5888.250 kb on + strand, within Pf6N2E2_5105at 5888.250 kb on + strand, within Pf6N2E2_5105at 5888.251 kb on - strand, within Pf6N2E2_5105at 5888.322 kb on + strand, within Pf6N2E2_5105at 5888.322 kb on + strand, within Pf6N2E2_5105at 5888.323 kb on - strand, within Pf6N2E2_5105at 5888.323 kb on - strand, within Pf6N2E2_5105at 5888.333 kb on + strand, within Pf6N2E2_5105at 5888.421 kb on + strand, within Pf6N2E2_5105at 5888.421 kb on + strand, within Pf6N2E2_5105at 5888.422 kb on - strand, within Pf6N2E2_5105at 5888.422 kb on - strand, within Pf6N2E2_5105at 5888.422 kb on - strand, within Pf6N2E2_5105at 5888.425 kb on - strand, within Pf6N2E2_5105at 5888.425 kb on - strand, within Pf6N2E2_5105at 5888.445 kb on + strand, within Pf6N2E2_5105at 5888.445 kb on + strand, within Pf6N2E2_5105at 5888.446 kb on - strand, within Pf6N2E2_5105at 5888.496 kb on + strandat 5888.496 kb on + strandat 5888.496 kb on + strandat 5888.497 kb on - strandat 5888.497 kb on - strandat 5888.497 kb on - strandat 5888.505 kb on + strandat 5888.505 kb on + strandat 5888.511 kb on + strandat 5888.538 kb on + strandat 5888.538 kb on + strandat 5888.538 kb on + strandat 5888.538 kb on + strandat 5888.538 kb on + strandat 5888.539 kb on - strandat 5888.539 kb on - strandat 5888.539 kb on - strandat 5888.541 kb on + strandat 5888.541 kb on + strandat 5888.541 kb on + strandat 5888.541 kb on + strandat 5888.542 kb on - strandat 5888.542 kb on - strandat 5888.542 kb on - strandat 5888.542 kb on - strandat 5888.544 kb on + strandat 5888.544 kb on + strandat 5888.544 kb on + strandat 5888.545 kb on - strandat 5888.596 kb on + strandat 5888.596 kb on + strandat 5888.596 kb on + strandat 5888.596 kb on + strandat 5888.607 kb on + strandat 5888.607 kb on + strandat 5888.607 kb on + strandat 5888.607 kb on + strandat 5888.608 kb on - strandat 5888.608 kb on - strandat 5888.608 kb on - strandat 5888.616 kb on + strandat 5888.616 kb on + strandat 5888.616 kb on + strandat 5888.698 kb on - strandat 5888.698 kb on - strandat 5888.734 kb on + strandat 5888.734 kb on + strandat 5888.734 kb on + strandat 5888.734 kb on + strandat 5888.735 kb on - strandat 5888.795 kb on + strandat 5888.795 kb on + strandat 5888.795 kb on + strandat 5888.796 kb on - strandat 5888.796 kb on - strandat 5889.344 kb on + strandat 5889.393 kb on + strandat 5889.393 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM
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5,885,846 + Pf6N2E2_5103 0.76 -2.7
5,885,846 + Pf6N2E2_5103 0.76 +1.5
5,886,287 - Pf6N2E2_5104 0.36 -1.7
5,886,381 + Pf6N2E2_5104 0.56 +0.0
5,886,381 + Pf6N2E2_5104 0.56 +1.5
5,886,381 + Pf6N2E2_5104 0.56 +1.2
5,886,489 + Pf6N2E2_5104 0.80 -0.9
5,886,489 + Pf6N2E2_5104 0.80 +0.3
5,886,490 - Pf6N2E2_5104 0.80 +0.8
5,886,490 - Pf6N2E2_5104 0.80 +0.0
5,886,500 - Pf6N2E2_5104 0.82 -0.2
5,886,500 - Pf6N2E2_5104 0.82 -0.0
5,886,508 + Pf6N2E2_5104 0.84 +0.3
5,886,508 + Pf6N2E2_5104 0.84 -0.1
5,886,577 + +0.5
5,886,578 - +1.2
5,886,617 + +0.4
5,886,621 + +0.3
5,886,622 - +0.0
5,886,659 + -0.0
5,886,660 - -4.7
5,886,660 - +0.5
5,886,685 + +0.5
5,886,730 - +0.1
5,886,760 + -0.3
5,886,797 + -0.8
5,886,820 + -0.0
5,886,820 + -0.1
5,886,820 + +0.1
5,886,826 - -1.2
5,886,826 - -0.2
5,886,876 + Pf6N2E2_5105 0.11 -1.7
5,886,876 + Pf6N2E2_5105 0.11 +1.3
5,886,876 + Pf6N2E2_5105 0.11 -1.1
5,886,877 - Pf6N2E2_5105 0.11 -0.8
5,886,906 + Pf6N2E2_5105 0.12 -0.8
5,886,906 + Pf6N2E2_5105 0.12 +1.4
5,886,907 - Pf6N2E2_5105 0.12 +1.1
5,887,120 - Pf6N2E2_5105 0.23 -0.3
5,887,194 + Pf6N2E2_5105 0.26 -1.1
5,887,194 + Pf6N2E2_5105 0.26 -0.0
5,887,261 - Pf6N2E2_5105 0.29 -0.7
5,887,308 + Pf6N2E2_5105 0.32 -0.1
5,887,308 + Pf6N2E2_5105 0.32 +0.9
5,887,308 + Pf6N2E2_5105 0.32 +0.6
5,887,308 + Pf6N2E2_5105 0.32 +1.5
5,887,309 - Pf6N2E2_5105 0.32 +0.4
5,887,309 - Pf6N2E2_5105 0.32 +0.3
5,887,309 - Pf6N2E2_5105 0.32 -0.3
5,887,350 + Pf6N2E2_5105 0.34 -0.4
5,887,392 + Pf6N2E2_5105 0.36 -1.0
5,887,393 - Pf6N2E2_5105 0.36 -0.3
5,887,393 - Pf6N2E2_5105 0.36 +0.4
5,887,393 - Pf6N2E2_5105 0.36 -1.1
5,887,393 - Pf6N2E2_5105 0.36 +0.7
5,887,458 + Pf6N2E2_5105 0.39 -1.5
5,887,458 + Pf6N2E2_5105 0.39 -0.1
5,887,458 + Pf6N2E2_5105 0.39 +1.4
5,887,458 + Pf6N2E2_5105 0.39 +1.5
5,887,459 - Pf6N2E2_5105 0.39 -0.1
5,887,459 - Pf6N2E2_5105 0.39 +1.5
5,887,459 - Pf6N2E2_5105 0.39 +0.7
5,887,459 - Pf6N2E2_5105 0.39 +0.2
5,887,459 - Pf6N2E2_5105 0.39 +0.2
5,887,459 - Pf6N2E2_5105 0.39 +0.5
5,887,459 - Pf6N2E2_5105 0.39 -0.1
5,887,527 + Pf6N2E2_5105 0.42 +0.9
5,887,527 + Pf6N2E2_5105 0.42 +0.6
5,887,527 + Pf6N2E2_5105 0.42 +2.3
5,887,527 + Pf6N2E2_5105 0.42 +0.8
5,887,527 + Pf6N2E2_5105 0.42 +0.4
5,887,527 + Pf6N2E2_5105 0.42 +1.0
5,887,527 + Pf6N2E2_5105 0.42 -0.0
5,887,528 - Pf6N2E2_5105 0.43 -1.7
5,887,528 - Pf6N2E2_5105 0.43 +0.5
5,887,528 - Pf6N2E2_5105 0.43 -0.1
5,887,528 - Pf6N2E2_5105 0.43 +0.4
5,887,528 - Pf6N2E2_5105 0.43 +0.2
5,887,608 + Pf6N2E2_5105 0.46 -1.0
5,887,608 + Pf6N2E2_5105 0.46 -0.5
5,887,608 + Pf6N2E2_5105 0.46 -1.5
5,887,671 + Pf6N2E2_5105 0.50 -1.5
5,887,671 + Pf6N2E2_5105 0.50 -0.1
5,887,671 + Pf6N2E2_5105 0.50 +0.5
5,887,671 + Pf6N2E2_5105 0.50 -0.4
5,887,671 + Pf6N2E2_5105 0.50 -2.3
5,887,671 + Pf6N2E2_5105 0.50 +0.4
5,887,671 + Pf6N2E2_5105 0.50 +1.4
5,887,672 - Pf6N2E2_5105 0.50 -0.3
5,887,672 - Pf6N2E2_5105 0.50 +1.5
5,887,672 - Pf6N2E2_5105 0.50 -1.8
5,887,672 - Pf6N2E2_5105 0.50 +0.3
5,887,672 - Pf6N2E2_5105 0.50 +0.2
5,887,794 + Pf6N2E2_5105 0.56 +0.9
5,887,972 - Pf6N2E2_5105 0.64 +0.3
5,887,995 + Pf6N2E2_5105 0.65 -0.1
5,888,007 + Pf6N2E2_5105 0.66 -0.1
5,888,007 + Pf6N2E2_5105 0.66 +0.2
5,888,008 - Pf6N2E2_5105 0.66 -0.5
5,888,008 - Pf6N2E2_5105 0.66 +1.5
5,888,043 + Pf6N2E2_5105 0.68 +2.3
5,888,043 + Pf6N2E2_5105 0.68 +0.3
5,888,043 + Pf6N2E2_5105 0.68 -0.7
5,888,044 - Pf6N2E2_5105 0.68 -0.0
5,888,075 + Pf6N2E2_5105 0.69 +0.2
5,888,118 + Pf6N2E2_5105 0.71 +0.3
5,888,119 - Pf6N2E2_5105 0.72 -0.8
5,888,119 - Pf6N2E2_5105 0.72 -2.0
5,888,141 + Pf6N2E2_5105 0.73 +0.5
5,888,154 + Pf6N2E2_5105 0.73 +1.2
5,888,154 + Pf6N2E2_5105 0.73 -1.5
5,888,154 + Pf6N2E2_5105 0.73 -0.4
5,888,154 + Pf6N2E2_5105 0.73 +1.2
5,888,154 + Pf6N2E2_5105 0.73 -1.2
5,888,154 + Pf6N2E2_5105 0.73 -1.7
5,888,154 + Pf6N2E2_5105 0.73 -0.2
5,888,155 - Pf6N2E2_5105 0.73 -0.8
5,888,155 - Pf6N2E2_5105 0.73 -3.4
5,888,226 + Pf6N2E2_5105 0.77 +0.6
5,888,226 + Pf6N2E2_5105 0.77 +0.6
5,888,226 + Pf6N2E2_5105 0.77 +0.8
5,888,226 + Pf6N2E2_5105 0.77 +1.6
5,888,226 + Pf6N2E2_5105 0.77 -0.3
5,888,226 + Pf6N2E2_5105 0.77 +1.1
5,888,226 + Pf6N2E2_5105 0.77 +1.5
5,888,227 - Pf6N2E2_5105 0.77 +1.5
5,888,227 - Pf6N2E2_5105 0.77 +2.0
5,888,227 - Pf6N2E2_5105 0.77 +0.2
5,888,227 - Pf6N2E2_5105 0.77 -1.1
5,888,227 - Pf6N2E2_5105 0.77 -0.9
5,888,250 + Pf6N2E2_5105 0.78 +1.4
5,888,250 + Pf6N2E2_5105 0.78 +0.0
5,888,250 + Pf6N2E2_5105 0.78 +0.2
5,888,250 + Pf6N2E2_5105 0.78 +0.5
5,888,250 + Pf6N2E2_5105 0.78 +0.0
5,888,250 + Pf6N2E2_5105 0.78 -0.2
5,888,250 + Pf6N2E2_5105 0.78 -1.0
5,888,251 - Pf6N2E2_5105 0.78 -0.4
5,888,322 + Pf6N2E2_5105 0.81 -1.3
5,888,322 + Pf6N2E2_5105 0.81 -0.8
5,888,323 - Pf6N2E2_5105 0.82 -0.2
5,888,323 - Pf6N2E2_5105 0.82 -1.7
5,888,333 + Pf6N2E2_5105 0.82 -0.1
5,888,421 + Pf6N2E2_5105 0.86 +1.5
5,888,421 + Pf6N2E2_5105 0.86 -0.9
5,888,422 - Pf6N2E2_5105 0.86 -2.5
5,888,422 - Pf6N2E2_5105 0.86 +0.5
5,888,422 - Pf6N2E2_5105 0.86 +0.4
5,888,425 - Pf6N2E2_5105 0.87 -0.1
5,888,425 - Pf6N2E2_5105 0.87 +2.5
5,888,445 + Pf6N2E2_5105 0.88 +0.3
5,888,445 + Pf6N2E2_5105 0.88 -0.9
5,888,446 - Pf6N2E2_5105 0.88 +0.9
5,888,496 + -0.7
5,888,496 + -0.5
5,888,496 + +0.9
5,888,497 - +0.6
5,888,497 - -1.8
5,888,497 - +0.5
5,888,505 + +0.3
5,888,505 + +1.5
5,888,511 + -0.2
5,888,538 + +0.5
5,888,538 + -0.6
5,888,538 + -0.2
5,888,538 + -0.2
5,888,538 + +1.1
5,888,539 - -1.0
5,888,539 - -0.7
5,888,539 - +1.3
5,888,541 + -2.2
5,888,541 + +0.3
5,888,541 + -0.2
5,888,541 + +0.8
5,888,542 - -0.0
5,888,542 - +1.0
5,888,542 - -0.4
5,888,542 - -1.5
5,888,544 + -0.5
5,888,544 + +0.2
5,888,544 + -0.3
5,888,545 - +2.7
5,888,596 + +0.1
5,888,596 + -0.8
5,888,596 + +0.3
5,888,596 + +1.3
5,888,607 + -0.3
5,888,607 + -1.1
5,888,607 + -0.6
5,888,607 + +0.0
5,888,608 - -0.1
5,888,608 - +0.9
5,888,608 - +1.5
5,888,616 + +1.5
5,888,616 + +1.1
5,888,616 + +1.5
5,888,698 - -0.1
5,888,698 - -0.5
5,888,734 + +0.6
5,888,734 + +0.0
5,888,734 + -0.3
5,888,734 + +2.0
5,888,735 - +1.5
5,888,795 + -0.1
5,888,795 + -0.1
5,888,795 + -0.0
5,888,796 - -1.8
5,888,796 - -1.1
5,889,344 + +1.5
5,889,393 + +0.5
5,889,393 + +1.5

Or see this region's nucleotide sequence