Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4559 and Pf6N2E2_4560 are separated by 4 nucleotides Pf6N2E2_4560 and Pf6N2E2_4561 are separated by 86 nucleotides Pf6N2E2_4561 and Pf6N2E2_4562 are separated by 144 nucleotides
Pf6N2E2_4559 - probable integral membrane protein NMA1898, at 5,287,585 to 5,288,946
_4559
Pf6N2E2_4560 - probable integral membrane protein NMA1899, at 5,288,951 to 5,290,318
_4560
Pf6N2E2_4561 - Dihydrofolate reductase (EC 1.5.1.3), at 5,290,405 to 5,290,917
_4561
Pf6N2E2_4562 - L-cystine uptake protein TcyP, at 5,291,062 to 5,292,453
_4562
Position (kb)
5288
5289
5290
5291 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5288.372 kb on + strand, within Pf6N2E2_4559 at 5288.372 kb on + strand, within Pf6N2E2_4559 at 5288.373 kb on - strand, within Pf6N2E2_4559 at 5288.552 kb on + strand, within Pf6N2E2_4559 at 5288.552 kb on + strand, within Pf6N2E2_4559 at 5288.553 kb on - strand, within Pf6N2E2_4559 at 5288.553 kb on - strand, within Pf6N2E2_4559 at 5288.553 kb on - strand, within Pf6N2E2_4559 at 5288.588 kb on + strand, within Pf6N2E2_4559 at 5288.588 kb on + strand, within Pf6N2E2_4559 at 5288.588 kb on + strand, within Pf6N2E2_4559 at 5288.588 kb on + strand, within Pf6N2E2_4559 at 5288.589 kb on - strand, within Pf6N2E2_4559 at 5288.774 kb on + strand, within Pf6N2E2_4559 at 5288.774 kb on + strand, within Pf6N2E2_4559 at 5288.774 kb on + strand, within Pf6N2E2_4559 at 5288.775 kb on - strand, within Pf6N2E2_4559 at 5288.775 kb on - strand, within Pf6N2E2_4559 at 5288.775 kb on - strand, within Pf6N2E2_4559 at 5288.883 kb on + strand at 5288.883 kb on + strand at 5289.435 kb on + strand, within Pf6N2E2_4560 at 5289.531 kb on + strand, within Pf6N2E2_4560 at 5289.531 kb on + strand, within Pf6N2E2_4560 at 5289.532 kb on - strand, within Pf6N2E2_4560 at 5289.532 kb on - strand, within Pf6N2E2_4560 at 5289.706 kb on + strand, within Pf6N2E2_4560 at 5289.755 kb on + strand, within Pf6N2E2_4560 at 5289.755 kb on + strand, within Pf6N2E2_4560 at 5290.202 kb on + strand at 5290.280 kb on + strand at 5290.310 kb on + strand at 5290.310 kb on + strand at 5290.353 kb on - strand at 5290.392 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5291.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 5,288,372 + Pf6N2E2_4559 0.58 +0.8 5,288,372 + Pf6N2E2_4559 0.58 -2.2 5,288,373 - Pf6N2E2_4559 0.58 -0.9 5,288,552 + Pf6N2E2_4559 0.71 +1.5 5,288,552 + Pf6N2E2_4559 0.71 +1.5 5,288,553 - Pf6N2E2_4559 0.71 -2.3 5,288,553 - Pf6N2E2_4559 0.71 +0.2 5,288,553 - Pf6N2E2_4559 0.71 +0.8 5,288,588 + Pf6N2E2_4559 0.74 -3.5 5,288,588 + Pf6N2E2_4559 0.74 -0.2 5,288,588 + Pf6N2E2_4559 0.74 -1.3 5,288,588 + Pf6N2E2_4559 0.74 -0.5 5,288,589 - Pf6N2E2_4559 0.74 +1.2 5,288,774 + Pf6N2E2_4559 0.87 -2.0 5,288,774 + Pf6N2E2_4559 0.87 -0.7 5,288,774 + Pf6N2E2_4559 0.87 -0.1 5,288,775 - Pf6N2E2_4559 0.87 +0.4 5,288,775 - Pf6N2E2_4559 0.87 -0.9 5,288,775 - Pf6N2E2_4559 0.87 -0.7 5,288,883 + +0.6 5,288,883 + -0.9 5,289,435 + Pf6N2E2_4560 0.35 -0.1 5,289,531 + Pf6N2E2_4560 0.42 -2.5 5,289,531 + Pf6N2E2_4560 0.42 +0.1 5,289,532 - Pf6N2E2_4560 0.42 -1.6 5,289,532 - Pf6N2E2_4560 0.42 -1.3 5,289,706 + Pf6N2E2_4560 0.55 -1.2 5,289,755 + Pf6N2E2_4560 0.59 -1.2 5,289,755 + Pf6N2E2_4560 0.59 +3.1 5,290,202 + -0.1 5,290,280 + -0.8 5,290,310 + +0.8 5,290,310 + +0.9 5,290,353 - +1.0 5,290,392 + +0.4 5,290,926 + -0.3 5,290,926 + +1.5 5,290,926 + +0.4 5,290,926 + -1.8 5,290,926 + +0.8 5,290,926 + +0.9 5,290,927 - +1.0 5,290,927 - +0.1 5,290,927 - -0.6 5,290,927 - -0.2 5,290,927 - -0.9 5,291,071 + -0.4
Or see this region's nucleotide sequence