Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3986 and Pf6N2E2_3987 overlap by 4 nucleotides Pf6N2E2_3987 and Pf6N2E2_3988 are separated by 10 nucleotides
Pf6N2E2_3986 - PROBABLE REMNANT OF A TRANSPOSASE GENE PROTEIN, at 4,652,676 to 4,653,452
_3986
Pf6N2E2_3987 - FIG00953743: hypothetical protein, at 4,653,449 to 4,654,381
_3987
Pf6N2E2_3988 - Non-ribosomal peptide synthetase modules and related proteins, at 4,654,392 to 4,657,811
_3988
Position (kb)
4653
4654
4655 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4652.472 kb on + strand at 4652.698 kb on + strand at 4652.699 kb on - strand at 4652.908 kb on + strand, within Pf6N2E2_3986 at 4652.968 kb on - strand, within Pf6N2E2_3986 at 4653.250 kb on - strand, within Pf6N2E2_3986 at 4653.252 kb on + strand, within Pf6N2E2_3986 at 4653.252 kb on + strand, within Pf6N2E2_3986 at 4653.253 kb on - strand, within Pf6N2E2_3986 at 4653.253 kb on - strand, within Pf6N2E2_3986 at 4653.253 kb on - strand, within Pf6N2E2_3986 at 4653.253 kb on - strand, within Pf6N2E2_3986 at 4653.253 kb on - strand, within Pf6N2E2_3986 at 4653.253 kb on - strand, within Pf6N2E2_3986 at 4653.355 kb on + strand, within Pf6N2E2_3986 at 4653.355 kb on + strand, within Pf6N2E2_3986 at 4653.465 kb on - strand at 4653.465 kb on - strand at 4653.641 kb on + strand, within Pf6N2E2_3987 at 4653.641 kb on + strand, within Pf6N2E2_3987 at 4653.642 kb on - strand, within Pf6N2E2_3987 at 4653.642 kb on - strand, within Pf6N2E2_3987 at 4653.642 kb on - strand, within Pf6N2E2_3987 at 4653.642 kb on - strand, within Pf6N2E2_3987 at 4653.642 kb on - strand, within Pf6N2E2_3987 at 4653.782 kb on + strand, within Pf6N2E2_3987 at 4653.783 kb on - strand, within Pf6N2E2_3987 at 4653.783 kb on - strand, within Pf6N2E2_3987 at 4654.089 kb on - strand, within Pf6N2E2_3987 at 4654.089 kb on - strand, within Pf6N2E2_3987 at 4654.089 kb on - strand, within Pf6N2E2_3987 at 4654.157 kb on + strand, within Pf6N2E2_3987 at 4654.347 kb on + strand at 4654.368 kb on - strand at 4654.536 kb on - strand at 4654.610 kb on - strand at 4654.630 kb on + strand at 4654.998 kb on + strand, within Pf6N2E2_3988 at 4654.999 kb on - strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.113 kb on + strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.114 kb on - strand, within Pf6N2E2_3988 at 4655.115 kb on + strand, within Pf6N2E2_3988 at 4655.116 kb on - strand, within Pf6N2E2_3988 at 4655.116 kb on - strand, within Pf6N2E2_3988 at 4655.155 kb on - strand, within Pf6N2E2_3988 at 4655.367 kb on + strand, within Pf6N2E2_3988 at 4655.367 kb on + strand, within Pf6N2E2_3988 at 4655.367 kb on + strand, within Pf6N2E2_3988 at 4655.367 kb on + strand, within Pf6N2E2_3988 at 4655.367 kb on + strand, within Pf6N2E2_3988 at 4655.368 kb on - strand, within Pf6N2E2_3988 at 4655.368 kb on - strand, within Pf6N2E2_3988 at 4655.368 kb on - strand, within Pf6N2E2_3988
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 4,652,472 + -2.1 4,652,698 + +0.8 4,652,699 - +2.4 4,652,908 + Pf6N2E2_3986 0.30 -2.4 4,652,968 - Pf6N2E2_3986 0.38 -0.3 4,653,250 - Pf6N2E2_3986 0.74 -1.3 4,653,252 + Pf6N2E2_3986 0.74 +1.8 4,653,252 + Pf6N2E2_3986 0.74 -0.1 4,653,253 - Pf6N2E2_3986 0.74 -0.1 4,653,253 - Pf6N2E2_3986 0.74 -0.5 4,653,253 - Pf6N2E2_3986 0.74 +0.8 4,653,253 - Pf6N2E2_3986 0.74 +0.2 4,653,253 - Pf6N2E2_3986 0.74 -0.2 4,653,253 - Pf6N2E2_3986 0.74 +0.5 4,653,355 + Pf6N2E2_3986 0.87 +0.8 4,653,355 + Pf6N2E2_3986 0.87 +0.5 4,653,465 - -0.1 4,653,465 - +0.1 4,653,641 + Pf6N2E2_3987 0.21 +1.5 4,653,641 + Pf6N2E2_3987 0.21 -0.3 4,653,642 - Pf6N2E2_3987 0.21 -0.8 4,653,642 - Pf6N2E2_3987 0.21 +0.4 4,653,642 - Pf6N2E2_3987 0.21 +0.4 4,653,642 - Pf6N2E2_3987 0.21 +0.7 4,653,642 - Pf6N2E2_3987 0.21 -0.3 4,653,782 + Pf6N2E2_3987 0.36 -2.6 4,653,783 - Pf6N2E2_3987 0.36 -0.4 4,653,783 - Pf6N2E2_3987 0.36 -2.3 4,654,089 - Pf6N2E2_3987 0.69 +0.5 4,654,089 - Pf6N2E2_3987 0.69 -0.4 4,654,089 - Pf6N2E2_3987 0.69 -0.3 4,654,157 + Pf6N2E2_3987 0.76 -0.8 4,654,347 + +1.0 4,654,368 - -1.5 4,654,536 - +0.7 4,654,610 - -0.2 4,654,630 + -0.4 4,654,998 + Pf6N2E2_3988 0.18 -0.1 4,654,999 - Pf6N2E2_3988 0.18 +0.1 4,655,113 + Pf6N2E2_3988 0.21 -1.6 4,655,113 + Pf6N2E2_3988 0.21 +0.5 4,655,113 + Pf6N2E2_3988 0.21 +3.3 4,655,113 + Pf6N2E2_3988 0.21 -0.2 4,655,113 + Pf6N2E2_3988 0.21 +1.2 4,655,113 + Pf6N2E2_3988 0.21 -0.4 4,655,113 + Pf6N2E2_3988 0.21 +0.5 4,655,113 + Pf6N2E2_3988 0.21 -0.5 4,655,113 + Pf6N2E2_3988 0.21 +0.9 4,655,114 - Pf6N2E2_3988 0.21 -2.6 4,655,114 - Pf6N2E2_3988 0.21 +1.5 4,655,114 - Pf6N2E2_3988 0.21 -0.6 4,655,114 - Pf6N2E2_3988 0.21 +0.2 4,655,114 - Pf6N2E2_3988 0.21 +0.1 4,655,114 - Pf6N2E2_3988 0.21 +0.3 4,655,114 - Pf6N2E2_3988 0.21 +1.0 4,655,114 - Pf6N2E2_3988 0.21 -1.8 4,655,114 - Pf6N2E2_3988 0.21 +0.0 4,655,114 - Pf6N2E2_3988 0.21 -0.0 4,655,114 - Pf6N2E2_3988 0.21 -1.1 4,655,114 - Pf6N2E2_3988 0.21 -0.5 4,655,114 - Pf6N2E2_3988 0.21 +0.1 4,655,115 + Pf6N2E2_3988 0.21 +0.4 4,655,116 - Pf6N2E2_3988 0.21 +0.2 4,655,116 - Pf6N2E2_3988 0.21 +1.5 4,655,155 - Pf6N2E2_3988 0.22 +1.5 4,655,367 + Pf6N2E2_3988 0.29 -0.5 4,655,367 + Pf6N2E2_3988 0.29 -3.3 4,655,367 + Pf6N2E2_3988 0.29 -0.2 4,655,367 + Pf6N2E2_3988 0.29 +0.0 4,655,367 + Pf6N2E2_3988 0.29 +0.3 4,655,368 - Pf6N2E2_3988 0.29 -0.4 4,655,368 - Pf6N2E2_3988 0.29 +1.5 4,655,368 - Pf6N2E2_3988 0.29 -1.1
Or see this region's nucleotide sequence