Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1219 and Pf6N2E2_1220 are separated by 63 nucleotides Pf6N2E2_1220 and Pf6N2E2_1221 are separated by 319 nucleotides Pf6N2E2_1221 and Pf6N2E2_1222 are separated by 148 nucleotides Pf6N2E2_1222 and Pf6N2E2_1223 are separated by 153 nucleotides
Pf6N2E2_1219 - FIG00955186: hypothetical protein, at 1,425,925 to 1,427,115
_1219
Pf6N2E2_1220 - hypothetical protein, at 1,427,179 to 1,427,340
_1220
Pf6N2E2_1221 - sodium/hydrogen exchanger family protein, at 1,427,660 to 1,428,997
_1221
Pf6N2E2_1222 - FIG00954310: hypothetical protein, at 1,429,146 to 1,429,682
_1222
Pf6N2E2_1223 - Amino acid transporters, at 1,429,836 to 1,430,066
_1223
Position (kb)
1427
1428
1429 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1426.727 kb on + strand, within Pf6N2E2_1219 at 1426.727 kb on + strand, within Pf6N2E2_1219 at 1426.775 kb on + strand, within Pf6N2E2_1219 at 1426.775 kb on + strand, within Pf6N2E2_1219 at 1426.886 kb on + strand, within Pf6N2E2_1219 at 1426.886 kb on + strand, within Pf6N2E2_1219 at 1426.887 kb on - strand, within Pf6N2E2_1219 at 1426.887 kb on - strand, within Pf6N2E2_1219 at 1426.940 kb on + strand, within Pf6N2E2_1219 at 1426.940 kb on + strand, within Pf6N2E2_1219 at 1426.940 kb on + strand, within Pf6N2E2_1219 at 1426.941 kb on - strand, within Pf6N2E2_1219 at 1426.991 kb on + strand, within Pf6N2E2_1219 at 1426.991 kb on + strand, within Pf6N2E2_1219 at 1426.991 kb on + strand, within Pf6N2E2_1219 at 1426.992 kb on - strand, within Pf6N2E2_1219 at 1426.992 kb on - strand, within Pf6N2E2_1219 at 1427.063 kb on + strand at 1427.064 kb on - strand at 1427.064 kb on - strand at 1427.064 kb on - strand at 1427.231 kb on + strand, within Pf6N2E2_1220 at 1427.231 kb on + strand, within Pf6N2E2_1220 at 1427.232 kb on - strand, within Pf6N2E2_1220 at 1427.232 kb on - strand, within Pf6N2E2_1220 at 1427.260 kb on - strand, within Pf6N2E2_1220 at 1427.310 kb on + strand, within Pf6N2E2_1220 at 1427.727 kb on - strand at 1427.727 kb on - strand at 1427.727 kb on - strand at 1427.936 kb on + strand, within Pf6N2E2_1221 at 1427.996 kb on + strand, within Pf6N2E2_1221 at 1427.996 kb on + strand, within Pf6N2E2_1221 at 1428.052 kb on + strand, within Pf6N2E2_1221 at 1428.053 kb on - strand, within Pf6N2E2_1221 at 1428.353 kb on + strand, within Pf6N2E2_1221 at 1428.354 kb on - strand, within Pf6N2E2_1221 at 1428.384 kb on - strand, within Pf6N2E2_1221 at 1428.842 kb on + strand, within Pf6N2E2_1221 at 1428.842 kb on + strand, within Pf6N2E2_1221 at 1428.971 kb on + strand at 1428.971 kb on + strand at 1428.971 kb on + strand at 1428.972 kb on - strand at 1428.999 kb on + strand at 1429.173 kb on - strand at 1429.174 kb on + strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.183 kb on + strand at 1429.183 kb on + strand at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.304 kb on - strand, within Pf6N2E2_1222 at 1429.335 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.672 kb on - strand at 1429.720 kb on - strand at 1429.809 kb on - strand at 1429.809 kb on - strand at 1429.809 kb on - strand at 1429.843 kb on - strand at 1429.946 kb on + strand, within Pf6N2E2_1223 at 1429.958 kb on + strand, within Pf6N2E2_1223 at 1429.958 kb on + strand, within Pf6N2E2_1223 at 1429.985 kb on + strand, within Pf6N2E2_1223
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 1,426,727 + Pf6N2E2_1219 0.67 +0.3 1,426,727 + Pf6N2E2_1219 0.67 +1.1 1,426,775 + Pf6N2E2_1219 0.71 -0.8 1,426,775 + Pf6N2E2_1219 0.71 +0.4 1,426,886 + Pf6N2E2_1219 0.81 -1.9 1,426,886 + Pf6N2E2_1219 0.81 -0.1 1,426,887 - Pf6N2E2_1219 0.81 +1.4 1,426,887 - Pf6N2E2_1219 0.81 -0.1 1,426,940 + Pf6N2E2_1219 0.85 +0.5 1,426,940 + Pf6N2E2_1219 0.85 +1.4 1,426,940 + Pf6N2E2_1219 0.85 -1.3 1,426,941 - Pf6N2E2_1219 0.85 +1.4 1,426,991 + Pf6N2E2_1219 0.90 -0.1 1,426,991 + Pf6N2E2_1219 0.90 -0.6 1,426,991 + Pf6N2E2_1219 0.90 -0.4 1,426,992 - Pf6N2E2_1219 0.90 -0.1 1,426,992 - Pf6N2E2_1219 0.90 -1.3 1,427,063 + +1.4 1,427,064 - -0.6 1,427,064 - +0.1 1,427,064 - -0.6 1,427,231 + Pf6N2E2_1220 0.32 -1.6 1,427,231 + Pf6N2E2_1220 0.32 +0.4 1,427,232 - Pf6N2E2_1220 0.33 -0.8 1,427,232 - Pf6N2E2_1220 0.33 +1.6 1,427,260 - Pf6N2E2_1220 0.50 -0.2 1,427,310 + Pf6N2E2_1220 0.81 +0.2 1,427,727 - +1.4 1,427,727 - +0.8 1,427,727 - +1.4 1,427,936 + Pf6N2E2_1221 0.21 +0.9 1,427,996 + Pf6N2E2_1221 0.25 -0.1 1,427,996 + Pf6N2E2_1221 0.25 +0.5 1,428,052 + Pf6N2E2_1221 0.29 +0.8 1,428,053 - Pf6N2E2_1221 0.29 +2.4 1,428,353 + Pf6N2E2_1221 0.52 +0.6 1,428,354 - Pf6N2E2_1221 0.52 -0.1 1,428,384 - Pf6N2E2_1221 0.54 +0.8 1,428,842 + Pf6N2E2_1221 0.88 +0.4 1,428,842 + Pf6N2E2_1221 0.88 -0.8 1,428,971 + -1.2 1,428,971 + -0.1 1,428,971 + +0.1 1,428,972 - +1.2 1,428,999 + +1.0 1,429,173 - -3.1 1,429,174 + -1.7 1,429,175 - -0.9 1,429,175 - +1.4 1,429,175 - +0.3 1,429,175 - -0.3 1,429,183 + +0.4 1,429,183 + -2.3 1,429,226 + Pf6N2E2_1222 0.15 +0.1 1,429,226 + Pf6N2E2_1222 0.15 +1.7 1,429,226 + Pf6N2E2_1222 0.15 -1.3 1,429,226 + Pf6N2E2_1222 0.15 +1.4 1,429,226 + Pf6N2E2_1222 0.15 +0.4 1,429,227 - Pf6N2E2_1222 0.15 +0.6 1,429,227 - Pf6N2E2_1222 0.15 +1.5 1,429,227 - Pf6N2E2_1222 0.15 -2.6 1,429,304 - Pf6N2E2_1222 0.29 -0.1 1,429,335 + Pf6N2E2_1222 0.35 +1.4 1,429,534 + Pf6N2E2_1222 0.72 +0.0 1,429,534 + Pf6N2E2_1222 0.72 -0.0 1,429,534 + Pf6N2E2_1222 0.72 -0.1 1,429,534 + Pf6N2E2_1222 0.72 -0.4 1,429,534 + Pf6N2E2_1222 0.72 -0.9 1,429,535 - Pf6N2E2_1222 0.72 -3.0 1,429,535 - Pf6N2E2_1222 0.72 +0.9 1,429,535 - Pf6N2E2_1222 0.72 +1.6 1,429,672 - -0.4 1,429,720 - +1.4 1,429,809 - +0.4 1,429,809 - -1.3 1,429,809 - +1.4 1,429,843 - -0.3 1,429,946 + Pf6N2E2_1223 0.48 -0.2 1,429,958 + Pf6N2E2_1223 0.53 +0.0 1,429,958 + Pf6N2E2_1223 0.53 -1.3 1,429,985 + Pf6N2E2_1223 0.65 +0.2
Or see this region's nucleotide sequence