Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_95 and Pf6N2E2_96 are separated by 4 nucleotides Pf6N2E2_96 and Pf6N2E2_97 are separated by 93 nucleotides Pf6N2E2_97 and Pf6N2E2_98 overlap by 4 nucleotides
Pf6N2E2_95 - putative oxygenase (putative secreted protein), at 140,697 to 141,782
_95
Pf6N2E2_96 - FIG011065: hypothetical protein, at 141,787 to 142,551
_96
Pf6N2E2_97 - putataive branched-chain amino acid transport protein AzlC, at 142,645 to 143,355
_97
Pf6N2E2_98 - putative transmembrane protein, at 143,352 to 143,660
_98
Position (kb) 141
142
143 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 140.809 kb on + strand, within Pf6N2E2_95 at 140.810 kb on - strand at 141.033 kb on + strand, within Pf6N2E2_95 at 141.148 kb on + strand, within Pf6N2E2_95 at 141.148 kb on + strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.292 kb on - strand, within Pf6N2E2_95 at 141.507 kb on + strand, within Pf6N2E2_95 at 141.507 kb on + strand, within Pf6N2E2_95 at 141.593 kb on - strand, within Pf6N2E2_95 at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 142.107 kb on - strand, within Pf6N2E2_96 at 142.107 kb on - strand, within Pf6N2E2_96 at 142.126 kb on + strand, within Pf6N2E2_96 at 142.127 kb on - strand, within Pf6N2E2_96 at 142.229 kb on - strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.391 kb on - strand, within Pf6N2E2_96 at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.529 kb on - strand at 142.560 kb on + strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.789 kb on - strand, within Pf6N2E2_97 at 142.800 kb on - strand, within Pf6N2E2_97 at 143.230 kb on - strand, within Pf6N2E2_97 at 143.392 kb on + strand, within Pf6N2E2_98 at 143.393 kb on - strand, within Pf6N2E2_98
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 140,809 + Pf6N2E2_95 0.10 -0.1 140,810 - +2.0 141,033 + Pf6N2E2_95 0.31 +0.9 141,148 + Pf6N2E2_95 0.42 -0.6 141,148 + Pf6N2E2_95 0.42 +0.6 141,149 - Pf6N2E2_95 0.42 -0.9 141,149 - Pf6N2E2_95 0.42 +1.0 141,149 - Pf6N2E2_95 0.42 +0.6 141,149 - Pf6N2E2_95 0.42 -0.7 141,149 - Pf6N2E2_95 0.42 -2.5 141,149 - Pf6N2E2_95 0.42 -2.2 141,292 - Pf6N2E2_95 0.55 -1.4 141,507 + Pf6N2E2_95 0.75 -0.4 141,507 + Pf6N2E2_95 0.75 -0.1 141,593 - Pf6N2E2_95 0.83 -2.2 141,806 + -0.5 141,806 + -1.2 141,806 + -1.1 141,806 + -0.3 141,806 + -2.2 141,806 + -1.7 141,806 + -0.8 141,807 - -2.6 141,807 - -4.1 141,807 - +0.6 141,807 - -2.0 141,807 - -0.2 141,807 - +2.0 141,807 - +0.4 141,807 - +2.0 141,807 - -0.6 142,107 - Pf6N2E2_96 0.42 +2.0 142,107 - Pf6N2E2_96 0.42 +1.0 142,126 + Pf6N2E2_96 0.44 -3.4 142,127 - Pf6N2E2_96 0.44 -1.8 142,229 - Pf6N2E2_96 0.58 -1.2 142,390 + Pf6N2E2_96 0.79 -3.3 142,390 + Pf6N2E2_96 0.79 -2.6 142,390 + Pf6N2E2_96 0.79 +0.4 142,391 - Pf6N2E2_96 0.79 -3.8 142,528 + +0.6 142,528 + -1.0 142,528 + -3.0 142,528 + +1.0 142,529 - +0.4 142,560 + +0.5 142,602 - -2.4 142,602 - +0.5 142,602 - -1.4 142,621 + -0.6 142,621 + -2.9 142,621 + -0.8 142,621 + -0.2 142,622 - -0.6 142,622 - +1.0 142,622 - -2.0 142,622 - -1.4 142,622 - -1.4 142,622 - -2.5 142,622 - -0.0 142,693 + +0.9 142,693 + -0.1 142,693 + -0.6 142,693 + -1.5 142,693 + +1.0 142,693 + -1.2 142,694 - -1.2 142,694 - +0.5 142,694 - -2.0 142,694 - +4.0 142,788 + Pf6N2E2_97 0.20 -0.6 142,788 + Pf6N2E2_97 0.20 +0.2 142,788 + Pf6N2E2_97 0.20 +0.1 142,788 + Pf6N2E2_97 0.20 -1.3 142,789 - Pf6N2E2_97 0.20 +2.0 142,800 - Pf6N2E2_97 0.22 -0.9 143,230 - Pf6N2E2_97 0.82 +1.4 143,392 + Pf6N2E2_98 0.13 -0.1 143,393 - Pf6N2E2_98 0.13 -0.6
Or see this region's nucleotide sequence