Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_683

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_681 and Pf6N2E2_682 are separated by 112 nucleotidesPf6N2E2_682 and Pf6N2E2_683 are separated by 203 nucleotidesPf6N2E2_683 and Pf6N2E2_684 are separated by 137 nucleotides Pf6N2E2_681 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 785,832 to 786,764 _681 Pf6N2E2_682 - Predicted regulator PutR for proline utilization, GntR family, at 786,877 to 787,548 _682 Pf6N2E2_683 - Probable MFS transporter, at 787,752 to 788,918 _683 Pf6N2E2_684 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 789,056 to 790,447 _684 Position (kb) 787 788 789Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 786.832 kb on - strandat 786.832 kb on - strandat 786.837 kb on + strandat 786.837 kb on + strandat 786.838 kb on - strandat 786.838 kb on - strandat 786.838 kb on - strandat 787.115 kb on + strand, within Pf6N2E2_682at 787.116 kb on - strand, within Pf6N2E2_682at 787.116 kb on - strand, within Pf6N2E2_682at 787.116 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.160 kb on - strand, within Pf6N2E2_682at 787.246 kb on + strand, within Pf6N2E2_682at 787.247 kb on - strand, within Pf6N2E2_682at 787.585 kb on + strandat 787.777 kb on + strandat 787.777 kb on + strandat 787.777 kb on + strandat 787.777 kb on + strandat 787.778 kb on - strandat 787.778 kb on - strandat 787.778 kb on - strandat 787.778 kb on - strandat 787.778 kb on - strandat 787.778 kb on - strandat 787.778 kb on - strandat 787.918 kb on + strand, within Pf6N2E2_683at 787.919 kb on - strand, within Pf6N2E2_683at 787.999 kb on + strand, within Pf6N2E2_683at 788.092 kb on + strand, within Pf6N2E2_683at 788.190 kb on - strand, within Pf6N2E2_683at 788.564 kb on + strand, within Pf6N2E2_683at 788.564 kb on + strand, within Pf6N2E2_683at 788.565 kb on - strand, within Pf6N2E2_683at 788.566 kb on + strand, within Pf6N2E2_683at 788.566 kb on + strand, within Pf6N2E2_683at 788.567 kb on - strand, within Pf6N2E2_683at 788.567 kb on - strand, within Pf6N2E2_683at 788.567 kb on - strand, within Pf6N2E2_683at 788.567 kb on - strand, within Pf6N2E2_683at 788.770 kb on + strand, within Pf6N2E2_683at 788.770 kb on + strand, within Pf6N2E2_683at 788.771 kb on - strand, within Pf6N2E2_683at 788.771 kb on - strand, within Pf6N2E2_683at 788.824 kb on + strandat 788.833 kb on + strandat 788.834 kb on - strandat 788.930 kb on - strandat 788.957 kb on + strandat 788.957 kb on + strandat 789.009 kb on + strandat 789.009 kb on + strandat 789.009 kb on + strandat 789.010 kb on - strandat 789.224 kb on + strand, within Pf6N2E2_684at 789.225 kb on - strand, within Pf6N2E2_684at 789.473 kb on + strand, within Pf6N2E2_684at 789.473 kb on + strand, within Pf6N2E2_684at 789.473 kb on + strand, within Pf6N2E2_684at 789.473 kb on + strand, within Pf6N2E2_684at 789.473 kb on + strand, within Pf6N2E2_684at 789.474 kb on - strand, within Pf6N2E2_684at 789.474 kb on - strand, within Pf6N2E2_684at 789.474 kb on - strand, within Pf6N2E2_684at 789.474 kb on - strand, within Pf6N2E2_684at 789.654 kb on - strand, within Pf6N2E2_684at 789.656 kb on + strand, within Pf6N2E2_684at 789.657 kb on - strand, within Pf6N2E2_684at 789.657 kb on - strand, within Pf6N2E2_684at 789.657 kb on - strandat 789.763 kb on + strand, within Pf6N2E2_684at 789.764 kb on - strand, within Pf6N2E2_684at 789.764 kb on - strand, within Pf6N2E2_684at 789.794 kb on + strand, within Pf6N2E2_684at 789.794 kb on + strand, within Pf6N2E2_684at 789.794 kb on + strand, within Pf6N2E2_684at 789.794 kb on + strand, within Pf6N2E2_684at 789.795 kb on - strand, within Pf6N2E2_684at 789.795 kb on - strand, within Pf6N2E2_684at 789.837 kb on - strand, within Pf6N2E2_684at 789.893 kb on + strand, within Pf6N2E2_684

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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786,832 - -1.9
786,832 - -0.5
786,837 + +1.8
786,837 + -0.2
786,838 - -0.7
786,838 - +0.1
786,838 - -0.7
787,115 + Pf6N2E2_682 0.35 -1.4
787,116 - Pf6N2E2_682 0.36 -0.8
787,116 - Pf6N2E2_682 0.36 -0.9
787,116 - Pf6N2E2_682 0.36 +1.4
787,160 - Pf6N2E2_682 0.42 +0.8
787,160 - Pf6N2E2_682 0.42 -0.2
787,160 - Pf6N2E2_682 0.42 -1.7
787,160 - Pf6N2E2_682 0.42 +1.8
787,246 + Pf6N2E2_682 0.55 +0.0
787,247 - Pf6N2E2_682 0.55 -0.1
787,585 + -1.2
787,777 + -0.1
787,777 + +0.8
787,777 + -3.1
787,777 + +1.8
787,778 - +1.8
787,778 - +0.2
787,778 - +0.2
787,778 - +0.1
787,778 - -0.9
787,778 - -0.1
787,778 - +0.3
787,918 + Pf6N2E2_683 0.14 -1.0
787,919 - Pf6N2E2_683 0.14 +0.5
787,999 + Pf6N2E2_683 0.21 +0.7
788,092 + Pf6N2E2_683 0.29 +0.2
788,190 - Pf6N2E2_683 0.38 -0.8
788,564 + Pf6N2E2_683 0.70 -0.5
788,564 + Pf6N2E2_683 0.70 +0.1
788,565 - Pf6N2E2_683 0.70 +1.8
788,566 + Pf6N2E2_683 0.70 -1.9
788,566 + Pf6N2E2_683 0.70 +0.8
788,567 - Pf6N2E2_683 0.70 -1.0
788,567 - Pf6N2E2_683 0.70 +0.6
788,567 - Pf6N2E2_683 0.70 -0.8
788,567 - Pf6N2E2_683 0.70 -2.1
788,770 + Pf6N2E2_683 0.87 +1.8
788,770 + Pf6N2E2_683 0.87 +1.8
788,771 - Pf6N2E2_683 0.87 -1.9
788,771 - Pf6N2E2_683 0.87 -1.3
788,824 + -4.0
788,833 + -0.2
788,834 - -1.8
788,930 - +0.5
788,957 + +0.2
788,957 + +0.6
789,009 + -0.0
789,009 + +0.7
789,009 + -1.3
789,010 - -0.4
789,224 + Pf6N2E2_684 0.12 +1.1
789,225 - Pf6N2E2_684 0.12 -0.6
789,473 + Pf6N2E2_684 0.30 -1.3
789,473 + Pf6N2E2_684 0.30 +1.8
789,473 + Pf6N2E2_684 0.30 +1.6
789,473 + Pf6N2E2_684 0.30 +0.8
789,473 + Pf6N2E2_684 0.30 +0.1
789,474 - Pf6N2E2_684 0.30 -0.8
789,474 - Pf6N2E2_684 0.30 -2.9
789,474 - Pf6N2E2_684 0.30 -1.8
789,474 - Pf6N2E2_684 0.30 +0.3
789,654 - Pf6N2E2_684 0.43 +1.8
789,656 + Pf6N2E2_684 0.43 -1.2
789,657 - Pf6N2E2_684 0.43 -1.5
789,657 - Pf6N2E2_684 0.43 -0.7
789,657 - +0.8
789,763 + Pf6N2E2_684 0.51 +0.2
789,764 - Pf6N2E2_684 0.51 -1.2
789,764 - Pf6N2E2_684 0.51 +0.4
789,794 + Pf6N2E2_684 0.53 +1.8
789,794 + Pf6N2E2_684 0.53 -0.2
789,794 + Pf6N2E2_684 0.53 -1.2
789,794 + Pf6N2E2_684 0.53 +1.8
789,795 - Pf6N2E2_684 0.53 +1.7
789,795 - Pf6N2E2_684 0.53 -0.0
789,837 - Pf6N2E2_684 0.56 -0.0
789,893 + Pf6N2E2_684 0.60 +0.2

Or see this region's nucleotide sequence