Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_63 and Pf6N2E2_64 are separated by 11 nucleotides Pf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides Pf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotides
Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428
_63
Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084
_64
Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987
_65
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Position (kb) 104
105
106 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 103.748 kb on + strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63 at 103.997 kb on + strand, within Pf6N2E2_63 at 103.998 kb on - strand, within Pf6N2E2_63 at 104.318 kb on - strand at 104.350 kb on + strand at 104.602 kb on + strand, within Pf6N2E2_64 at 104.603 kb on - strand, within Pf6N2E2_64 at 104.660 kb on - strand, within Pf6N2E2_64 at 104.774 kb on - strand, within Pf6N2E2_64 at 105.092 kb on + strand at 105.251 kb on + strand at 105.252 kb on - strand at 105.252 kb on - strand at 105.283 kb on + strand at 105.329 kb on + strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.437 kb on - strand, within Pf6N2E2_65 at 105.697 kb on + strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.801 kb on + strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.855 kb on + strand, within Pf6N2E2_65 at 105.954 kb on + strand at 105.955 kb on - strand at 106.013 kb on - strand at 106.013 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 103,748 + Pf6N2E2_63 0.52 -4.2 103,749 - Pf6N2E2_63 0.52 -1.7 103,749 - Pf6N2E2_63 0.52 -3.8 103,997 + Pf6N2E2_63 0.70 -0.9 103,998 - Pf6N2E2_63 0.70 -3.9 104,318 - -2.5 104,350 + +1.0 104,602 + Pf6N2E2_64 0.25 -4.0 104,603 - Pf6N2E2_64 0.25 -3.3 104,660 - Pf6N2E2_64 0.34 -4.8 104,774 - Pf6N2E2_64 0.52 -2.6 105,092 + -0.0 105,251 + -1.2 105,252 - -2.0 105,252 - -0.6 105,283 + -0.5 105,329 + Pf6N2E2_65 0.14 +0.2 105,330 - Pf6N2E2_65 0.14 -2.0 105,330 - Pf6N2E2_65 0.14 -2.4 105,330 - Pf6N2E2_65 0.14 -0.4 105,330 - Pf6N2E2_65 0.14 +1.0 105,330 - Pf6N2E2_65 0.14 -2.0 105,436 + Pf6N2E2_65 0.28 +0.6 105,436 + Pf6N2E2_65 0.28 +0.8 105,437 - Pf6N2E2_65 0.28 -0.4 105,697 + Pf6N2E2_65 0.62 +0.3 105,698 - Pf6N2E2_65 0.62 +0.0 105,698 - Pf6N2E2_65 0.62 +0.8 105,801 + Pf6N2E2_65 0.76 +0.2 105,802 - Pf6N2E2_65 0.76 -2.6 105,802 - Pf6N2E2_65 0.76 +1.0 105,829 + Pf6N2E2_65 0.79 +2.0 105,829 + Pf6N2E2_65 0.79 +0.3 105,855 + Pf6N2E2_65 0.83 +0.2 105,954 + +1.2 105,955 - +2.0 106,013 - -3.7 106,013 - -0.6
Or see this region's nucleotide sequence