Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5786 and Pf6N2E2_5787 overlap by 4 nucleotides Pf6N2E2_5787 and Pf6N2E2_5788 overlap by 14 nucleotides Pf6N2E2_5788 and Pf6N2E2_5789 overlap by 4 nucleotides Pf6N2E2_5789 and Pf6N2E2_5790 are separated by 121 nucleotides
Pf6N2E2_5786 - ABC transporter, ATP-binding protein, at 6,604,713 to 6,605,702
_5786
Pf6N2E2_5787 - Thiamin ABC transporter, transmembrane component, at 6,605,699 to 6,606,499
_5787
Pf6N2E2_5788 - ABC transporter permease protein, at 6,606,486 to 6,607,331
_5788
Pf6N2E2_5789 - FIG00956482: hypothetical protein, at 6,607,328 to 6,608,134
_5789
Pf6N2E2_5790 - ABC-type Fe3+ transport system, periplasmic component, at 6,608,256 to 6,609,323
_5790
Position (kb)
6606
6607
6608 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6605.695 kb on + strand at 6605.695 kb on + strand at 6605.696 kb on - strand at 6605.763 kb on + strand at 6605.763 kb on + strand at 6605.838 kb on + strand, within Pf6N2E2_5787 at 6605.940 kb on - strand, within Pf6N2E2_5787 at 6606.069 kb on + strand, within Pf6N2E2_5787 at 6606.070 kb on - strand, within Pf6N2E2_5787 at 6606.070 kb on - strand, within Pf6N2E2_5787 at 6606.123 kb on + strand, within Pf6N2E2_5787 at 6606.123 kb on + strand, within Pf6N2E2_5787 at 6606.124 kb on - strand, within Pf6N2E2_5787 at 6606.124 kb on - strand, within Pf6N2E2_5787 at 6606.124 kb on - strand, within Pf6N2E2_5787 at 6606.220 kb on - strand, within Pf6N2E2_5787 at 6606.339 kb on + strand, within Pf6N2E2_5787 at 6606.340 kb on - strand, within Pf6N2E2_5787 at 6606.399 kb on + strand, within Pf6N2E2_5787 at 6606.399 kb on + strand, within Pf6N2E2_5787 at 6606.400 kb on - strand, within Pf6N2E2_5787 at 6606.400 kb on - strand, within Pf6N2E2_5787 at 6606.400 kb on - strand, within Pf6N2E2_5787 at 6606.400 kb on - strand, within Pf6N2E2_5787 at 6606.447 kb on + strand at 6606.448 kb on - strand at 6606.465 kb on + strand at 6606.465 kb on + strand at 6606.466 kb on - strand at 6606.466 kb on - strand at 6606.502 kb on + strand at 6606.502 kb on + strand at 6606.502 kb on + strand at 6606.502 kb on + strand at 6606.502 kb on + strand at 6606.502 kb on + strand at 6606.502 kb on + strand at 6606.502 kb on + strand at 6606.503 kb on - strand at 6606.503 kb on - strand at 6606.503 kb on - strand at 6606.503 kb on - strand at 6606.503 kb on - strand at 6606.503 kb on - strand at 6606.667 kb on + strand, within Pf6N2E2_5788 at 6606.667 kb on + strand, within Pf6N2E2_5788 at 6606.668 kb on - strand, within Pf6N2E2_5788 at 6606.668 kb on - strand, within Pf6N2E2_5788 at 6606.680 kb on - strand, within Pf6N2E2_5788 at 6606.680 kb on - strand, within Pf6N2E2_5788 at 6606.868 kb on + strand, within Pf6N2E2_5788 at 6606.868 kb on + strand, within Pf6N2E2_5788 at 6606.868 kb on + strand, within Pf6N2E2_5788 at 6606.868 kb on + strand, within Pf6N2E2_5788 at 6606.868 kb on + strand, within Pf6N2E2_5788 at 6606.869 kb on - strand, within Pf6N2E2_5788 at 6606.869 kb on - strand, within Pf6N2E2_5788 at 6606.869 kb on - strand, within Pf6N2E2_5788 at 6606.874 kb on + strand, within Pf6N2E2_5788 at 6607.075 kb on + strand, within Pf6N2E2_5788 at 6607.075 kb on + strand, within Pf6N2E2_5788 at 6607.076 kb on - strand, within Pf6N2E2_5788 at 6607.076 kb on - strand, within Pf6N2E2_5788 at 6607.076 kb on - strand, within Pf6N2E2_5788 at 6607.076 kb on - strand, within Pf6N2E2_5788 at 6607.156 kb on + strand, within Pf6N2E2_5788 at 6607.156 kb on + strand, within Pf6N2E2_5788 at 6607.337 kb on + strand at 6607.338 kb on - strand at 6607.350 kb on + strand at 6607.351 kb on - strand at 6607.569 kb on + strand, within Pf6N2E2_5789 at 6607.569 kb on + strand, within Pf6N2E2_5789 at 6607.570 kb on - strand, within Pf6N2E2_5789 at 6607.689 kb on + strand, within Pf6N2E2_5789 at 6607.689 kb on + strand, within Pf6N2E2_5789 at 6607.690 kb on - strand, within Pf6N2E2_5789 at 6607.690 kb on - strand, within Pf6N2E2_5789 at 6607.752 kb on + strand, within Pf6N2E2_5789 at 6607.830 kb on + strand, within Pf6N2E2_5789 at 6607.830 kb on + strand, within Pf6N2E2_5789 at 6607.830 kb on + strand, within Pf6N2E2_5789 at 6607.830 kb on + strand, within Pf6N2E2_5789 at 6607.831 kb on - strand, within Pf6N2E2_5789 at 6607.851 kb on + strand, within Pf6N2E2_5789 at 6607.852 kb on - strand, within Pf6N2E2_5789 at 6608.025 kb on + strand, within Pf6N2E2_5789 at 6608.025 kb on + strand, within Pf6N2E2_5789 at 6608.052 kb on + strand, within Pf6N2E2_5789 at 6608.052 kb on + strand, within Pf6N2E2_5789 at 6608.052 kb on + strand, within Pf6N2E2_5789 at 6608.053 kb on - strand, within Pf6N2E2_5789 at 6608.053 kb on - strand, within Pf6N2E2_5789 at 6608.053 kb on - strand, within Pf6N2E2_5789 at 6608.264 kb on + strand at 6608.264 kb on + strand at 6608.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 6,605,695 + +0.6 6,605,695 + +0.2 6,605,696 - +0.6 6,605,763 + -0.7 6,605,763 + -0.6 6,605,838 + Pf6N2E2_5787 0.17 -1.1 6,605,940 - Pf6N2E2_5787 0.30 -0.5 6,606,069 + Pf6N2E2_5787 0.46 -1.2 6,606,070 - Pf6N2E2_5787 0.46 +0.4 6,606,070 - Pf6N2E2_5787 0.46 -0.3 6,606,123 + Pf6N2E2_5787 0.53 -0.4 6,606,123 + Pf6N2E2_5787 0.53 +1.0 6,606,124 - Pf6N2E2_5787 0.53 -0.4 6,606,124 - Pf6N2E2_5787 0.53 -0.6 6,606,124 - Pf6N2E2_5787 0.53 +2.0 6,606,220 - Pf6N2E2_5787 0.65 +0.5 6,606,339 + Pf6N2E2_5787 0.80 -0.0 6,606,340 - Pf6N2E2_5787 0.80 +2.0 6,606,399 + Pf6N2E2_5787 0.87 -0.4 6,606,399 + Pf6N2E2_5787 0.87 -1.1 6,606,400 - Pf6N2E2_5787 0.88 -1.4 6,606,400 - Pf6N2E2_5787 0.88 +0.1 6,606,400 - Pf6N2E2_5787 0.88 -1.3 6,606,400 - Pf6N2E2_5787 0.88 -1.9 6,606,447 + +0.2 6,606,448 - -0.2 6,606,465 + -1.9 6,606,465 + +0.7 6,606,466 - -1.1 6,606,466 - +0.7 6,606,502 + +1.0 6,606,502 + +0.0 6,606,502 + +1.0 6,606,502 + +0.5 6,606,502 + +1.6 6,606,502 + -0.4 6,606,502 + -0.8 6,606,502 + -0.0 6,606,503 - -0.7 6,606,503 - +1.0 6,606,503 - -0.3 6,606,503 - +0.4 6,606,503 - +0.3 6,606,503 - +0.2 6,606,667 + Pf6N2E2_5788 0.21 -1.0 6,606,667 + Pf6N2E2_5788 0.21 -1.3 6,606,668 - Pf6N2E2_5788 0.22 +0.3 6,606,668 - Pf6N2E2_5788 0.22 +0.4 6,606,680 - Pf6N2E2_5788 0.23 -0.4 6,606,680 - Pf6N2E2_5788 0.23 -0.5 6,606,868 + Pf6N2E2_5788 0.45 -1.4 6,606,868 + Pf6N2E2_5788 0.45 -1.4 6,606,868 + Pf6N2E2_5788 0.45 -0.9 6,606,868 + Pf6N2E2_5788 0.45 -0.8 6,606,868 + Pf6N2E2_5788 0.45 -1.1 6,606,869 - Pf6N2E2_5788 0.45 +1.0 6,606,869 - Pf6N2E2_5788 0.45 +0.4 6,606,869 - Pf6N2E2_5788 0.45 -0.9 6,606,874 + Pf6N2E2_5788 0.46 -3.0 6,607,075 + Pf6N2E2_5788 0.70 +2.0 6,607,075 + Pf6N2E2_5788 0.70 -0.7 6,607,076 - Pf6N2E2_5788 0.70 -2.5 6,607,076 - Pf6N2E2_5788 0.70 +2.0 6,607,076 - Pf6N2E2_5788 0.70 -0.7 6,607,076 - Pf6N2E2_5788 0.70 -2.9 6,607,156 + Pf6N2E2_5788 0.79 +2.0 6,607,156 + Pf6N2E2_5788 0.79 -2.4 6,607,337 + +0.4 6,607,338 - +2.0 6,607,350 + -1.7 6,607,351 - +2.0 6,607,569 + Pf6N2E2_5789 0.30 -0.2 6,607,569 + Pf6N2E2_5789 0.30 -2.8 6,607,570 - Pf6N2E2_5789 0.30 +0.9 6,607,689 + Pf6N2E2_5789 0.45 +0.3 6,607,689 + Pf6N2E2_5789 0.45 +1.2 6,607,690 - Pf6N2E2_5789 0.45 +0.4 6,607,690 - Pf6N2E2_5789 0.45 -1.7 6,607,752 + Pf6N2E2_5789 0.53 +0.1 6,607,830 + Pf6N2E2_5789 0.62 +0.4 6,607,830 + Pf6N2E2_5789 0.62 -0.5 6,607,830 + Pf6N2E2_5789 0.62 -1.6 6,607,830 + Pf6N2E2_5789 0.62 -0.5 6,607,831 - Pf6N2E2_5789 0.62 -0.7 6,607,851 + Pf6N2E2_5789 0.65 +2.0 6,607,852 - Pf6N2E2_5789 0.65 +0.4 6,608,025 + Pf6N2E2_5789 0.86 -2.8 6,608,025 + Pf6N2E2_5789 0.86 -0.5 6,608,052 + Pf6N2E2_5789 0.90 +2.0 6,608,052 + Pf6N2E2_5789 0.90 -2.1 6,608,052 + Pf6N2E2_5789 0.90 +0.5 6,608,053 - Pf6N2E2_5789 0.90 -0.4 6,608,053 - Pf6N2E2_5789 0.90 -3.2 6,608,053 - Pf6N2E2_5789 0.90 +0.6 6,608,264 + +0.8 6,608,264 + -0.4 6,608,265 - +1.0
Or see this region's nucleotide sequence