Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5150 and Pf6N2E2_5151 are separated by 342 nucleotides Pf6N2E2_5151 and Pf6N2E2_5152 are separated by 184 nucleotides
Pf6N2E2_5150 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 5,936,696 to 5,938,360
_5150
Pf6N2E2_5151 - Aspartate 1-decarboxylase (EC 4.1.1.11), at 5,938,703 to 5,939,083
_5151
Pf6N2E2_5152 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 5,939,268 to 5,940,128
_5152
Position (kb) 5938
5939
5940 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5937.810 kb on + strand, within Pf6N2E2_5150 at 5938.049 kb on + strand, within Pf6N2E2_5150 at 5938.194 kb on + strand at 5938.353 kb on + strand at 5938.353 kb on + strand at 5938.354 kb on - strand at 5938.354 kb on - strand at 5938.397 kb on + strand at 5938.397 kb on + strand at 5938.397 kb on + strand at 5938.398 kb on - strand at 5938.403 kb on - strand at 5938.473 kb on + strand at 5938.473 kb on + strand at 5938.473 kb on + strand at 5938.474 kb on - strand at 5938.474 kb on - strand at 5938.478 kb on + strand at 5938.479 kb on - strand at 5938.479 kb on - strand at 5938.479 kb on - strand at 5938.536 kb on + strand at 5938.536 kb on + strand at 5938.582 kb on + strand at 5938.582 kb on + strand at 5938.582 kb on + strand at 5938.583 kb on - strand at 5938.583 kb on - strand at 5938.633 kb on - strand at 5938.633 kb on - strand at 5938.764 kb on + strand, within Pf6N2E2_5151 at 5938.764 kb on + strand, within Pf6N2E2_5151 at 5938.764 kb on + strand, within Pf6N2E2_5151 at 5938.764 kb on + strand, within Pf6N2E2_5151 at 5938.764 kb on + strand at 5938.765 kb on - strand, within Pf6N2E2_5151 at 5938.765 kb on - strand, within Pf6N2E2_5151 at 5938.765 kb on - strand, within Pf6N2E2_5151 at 5938.765 kb on - strand, within Pf6N2E2_5151 at 5938.765 kb on - strand, within Pf6N2E2_5151 at 5938.815 kb on + strand, within Pf6N2E2_5151 at 5938.911 kb on + strand, within Pf6N2E2_5151 at 5938.912 kb on - strand, within Pf6N2E2_5151 at 5938.912 kb on - strand, within Pf6N2E2_5151 at 5938.912 kb on - strand, within Pf6N2E2_5151 at 5938.912 kb on - strand, within Pf6N2E2_5151 at 5938.912 kb on - strand, within Pf6N2E2_5151 at 5938.912 kb on - strand, within Pf6N2E2_5151 at 5938.944 kb on + strand, within Pf6N2E2_5151 at 5938.945 kb on - strand, within Pf6N2E2_5151 at 5938.945 kb on - strand, within Pf6N2E2_5151 at 5938.945 kb on - strand, within Pf6N2E2_5151 at 5938.945 kb on - strand, within Pf6N2E2_5151 at 5938.962 kb on + strand, within Pf6N2E2_5151 at 5938.962 kb on + strand, within Pf6N2E2_5151 at 5938.963 kb on - strand, within Pf6N2E2_5151 at 5938.963 kb on - strand, within Pf6N2E2_5151 at 5939.019 kb on + strand, within Pf6N2E2_5151 at 5939.147 kb on + strand at 5939.147 kb on + strand at 5939.148 kb on - strand at 5939.269 kb on + strand at 5939.270 kb on - strand at 5939.270 kb on - strand at 5939.516 kb on - strand, within Pf6N2E2_5152
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 5,937,810 + Pf6N2E2_5150 0.67 +2.0 5,938,049 + Pf6N2E2_5150 0.81 -0.3 5,938,194 + +0.0 5,938,353 + -2.5 5,938,353 + -2.2 5,938,354 - -4.4 5,938,354 - +2.0 5,938,397 + -5.7 5,938,397 + -3.9 5,938,397 + -2.5 5,938,398 - +0.0 5,938,403 - -0.3 5,938,473 + -2.6 5,938,473 + -4.1 5,938,473 + -1.0 5,938,474 - -1.0 5,938,474 - +2.4 5,938,478 + +0.6 5,938,479 - -0.1 5,938,479 - -0.4 5,938,479 - +0.0 5,938,536 + -0.1 5,938,536 + -0.6 5,938,582 + +0.5 5,938,582 + +1.0 5,938,582 + +0.1 5,938,583 - -1.4 5,938,583 - +0.0 5,938,633 - -1.4 5,938,633 - +0.2 5,938,764 + Pf6N2E2_5151 0.16 -1.1 5,938,764 + Pf6N2E2_5151 0.16 -0.2 5,938,764 + Pf6N2E2_5151 0.16 +2.0 5,938,764 + Pf6N2E2_5151 0.16 -0.3 5,938,764 + +0.0 5,938,765 - Pf6N2E2_5151 0.16 -3.1 5,938,765 - Pf6N2E2_5151 0.16 -1.2 5,938,765 - Pf6N2E2_5151 0.16 +2.0 5,938,765 - Pf6N2E2_5151 0.16 -3.7 5,938,765 - Pf6N2E2_5151 0.16 -1.6 5,938,815 + Pf6N2E2_5151 0.29 -1.2 5,938,911 + Pf6N2E2_5151 0.55 +0.0 5,938,912 - Pf6N2E2_5151 0.55 -1.6 5,938,912 - Pf6N2E2_5151 0.55 +2.0 5,938,912 - Pf6N2E2_5151 0.55 +2.0 5,938,912 - Pf6N2E2_5151 0.55 -0.2 5,938,912 - Pf6N2E2_5151 0.55 +1.2 5,938,912 - Pf6N2E2_5151 0.55 -2.2 5,938,944 + Pf6N2E2_5151 0.63 -1.1 5,938,945 - Pf6N2E2_5151 0.64 -0.6 5,938,945 - Pf6N2E2_5151 0.64 +1.0 5,938,945 - Pf6N2E2_5151 0.64 -1.3 5,938,945 - Pf6N2E2_5151 0.64 +0.4 5,938,962 + Pf6N2E2_5151 0.68 +0.2 5,938,962 + Pf6N2E2_5151 0.68 -1.1 5,938,963 - Pf6N2E2_5151 0.68 -0.2 5,938,963 - Pf6N2E2_5151 0.68 -0.2 5,939,019 + Pf6N2E2_5151 0.83 -0.6 5,939,147 + -2.2 5,939,147 + +2.0 5,939,148 - -1.1 5,939,269 + +0.9 5,939,270 - -0.4 5,939,270 - +0.6 5,939,516 - Pf6N2E2_5152 0.29 +0.4
Or see this region's nucleotide sequence