Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4926 and Pf6N2E2_4927 are separated by 110 nucleotides Pf6N2E2_4927 and Pf6N2E2_4928 overlap by 4 nucleotides Pf6N2E2_4928 and Pf6N2E2_4929 overlap by 1 nucleotides Pf6N2E2_4929 and Pf6N2E2_4930 are separated by 65 nucleotides
Pf6N2E2_4926 - hypothetical protein, at 5,679,096 to 5,679,806
_4926
Pf6N2E2_4927 - GTP cyclohydrolase II (EC 3.5.4.25), at 5,679,917 to 5,680,534
_4927
Pf6N2E2_4928 - Permeases of the major facilitator superfamily, at 5,680,531 to 5,680,950
_4928
Pf6N2E2_4929 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 5,680,950 to 5,681,735
_4929
Pf6N2E2_4930 - Outer membrane vitamin B12 receptor BtuB, at 5,681,801 to 5,683,681
_4930
Position (kb)
5680
5681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5679.555 kb on + strand, within Pf6N2E2_4926 at 5679.556 kb on - strand, within Pf6N2E2_4926 at 5679.648 kb on + strand, within Pf6N2E2_4926 at 5679.680 kb on + strand, within Pf6N2E2_4926 at 5679.680 kb on + strand, within Pf6N2E2_4926 at 5679.680 kb on + strand, within Pf6N2E2_4926 at 5679.681 kb on - strand, within Pf6N2E2_4926 at 5679.768 kb on + strand at 5679.769 kb on - strand at 5679.814 kb on + strand at 5679.827 kb on + strand at 5679.843 kb on + strand at 5679.843 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.895 kb on + strand at 5679.895 kb on + strand at 5679.898 kb on + strand at 5679.898 kb on + strand at 5679.898 kb on + strand at 5680.579 kb on + strand, within Pf6N2E2_4928 at 5680.580 kb on - strand, within Pf6N2E2_4928 at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5681.122 kb on + strand, within Pf6N2E2_4929 at 5681.123 kb on - strand, within Pf6N2E2_4929 at 5681.257 kb on - strand, within Pf6N2E2_4929 at 5681.257 kb on - strand, within Pf6N2E2_4929 at 5681.257 kb on - strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.445 kb on + strand, within Pf6N2E2_4929 at 5681.446 kb on - strand, within Pf6N2E2_4929 at 5681.446 kb on - strand, within Pf6N2E2_4929 at 5681.707 kb on + strand at 5681.707 kb on + strand at 5681.707 kb on + strand at 5681.707 kb on + strand at 5681.708 kb on - strand at 5681.734 kb on - strand at 5681.881 kb on + strand at 5681.881 kb on + strand at 5681.883 kb on + strand at 5681.883 kb on + strand at 5681.883 kb on + strand at 5681.884 kb on - strand at 5681.884 kb on - strand at 5681.884 kb on - strand at 5681.884 kb on - strand at 5681.898 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 5,679,555 + Pf6N2E2_4926 0.65 +2.0 5,679,556 - Pf6N2E2_4926 0.65 -2.1 5,679,648 + Pf6N2E2_4926 0.78 +0.9 5,679,680 + Pf6N2E2_4926 0.82 -0.3 5,679,680 + Pf6N2E2_4926 0.82 -0.5 5,679,680 + Pf6N2E2_4926 0.82 +0.1 5,679,681 - Pf6N2E2_4926 0.82 +0.5 5,679,768 + +2.4 5,679,769 - -2.3 5,679,814 + -0.3 5,679,827 + -0.7 5,679,843 + +2.0 5,679,843 + -1.8 5,679,867 + -1.0 5,679,867 + -1.6 5,679,867 + +0.1 5,679,867 + +0.1 5,679,867 + +0.5 5,679,867 + +0.7 5,679,895 + -0.7 5,679,895 + +1.3 5,679,898 + -0.6 5,679,898 + -1.5 5,679,898 + -0.2 5,680,579 + Pf6N2E2_4928 0.11 -2.2 5,680,580 - Pf6N2E2_4928 0.12 +1.0 5,680,918 + -1.4 5,680,918 + +0.4 5,680,918 + -0.5 5,680,918 + -0.7 5,680,918 + -0.7 5,680,918 + +0.3 5,680,919 - -0.7 5,680,919 - -2.3 5,680,919 - -1.7 5,680,919 - -0.3 5,680,919 - -0.0 5,681,122 + Pf6N2E2_4929 0.22 +2.0 5,681,123 - Pf6N2E2_4929 0.22 -0.1 5,681,257 - Pf6N2E2_4929 0.39 -0.8 5,681,257 - Pf6N2E2_4929 0.39 -0.2 5,681,257 - Pf6N2E2_4929 0.39 -0.1 5,681,421 + Pf6N2E2_4929 0.60 +0.0 5,681,421 + Pf6N2E2_4929 0.60 +0.1 5,681,421 + Pf6N2E2_4929 0.60 +0.5 5,681,421 + Pf6N2E2_4929 0.60 +0.2 5,681,421 + Pf6N2E2_4929 0.60 -1.1 5,681,422 - Pf6N2E2_4929 0.60 -0.3 5,681,422 - Pf6N2E2_4929 0.60 -1.4 5,681,422 - Pf6N2E2_4929 0.60 +0.4 5,681,422 - Pf6N2E2_4929 0.60 -0.4 5,681,422 - Pf6N2E2_4929 0.60 -0.5 5,681,422 - Pf6N2E2_4929 0.60 -0.3 5,681,445 + Pf6N2E2_4929 0.63 +1.0 5,681,446 - Pf6N2E2_4929 0.63 -1.0 5,681,446 - Pf6N2E2_4929 0.63 +0.7 5,681,707 + -0.1 5,681,707 + +1.2 5,681,707 + -0.3 5,681,707 + -0.2 5,681,708 - +2.1 5,681,734 - +0.7 5,681,881 + +0.2 5,681,881 + +2.3 5,681,883 + +0.4 5,681,883 + -0.5 5,681,883 + +0.2 5,681,884 - -1.0 5,681,884 - -0.3 5,681,884 - +1.0 5,681,884 - -0.6 5,681,898 + +0.4
Or see this region's nucleotide sequence