Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3938

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3936 and Pf6N2E2_3937 are separated by 165 nucleotidesPf6N2E2_3937 and Pf6N2E2_3938 are separated by 137 nucleotidesPf6N2E2_3938 and Pf6N2E2_3939 are separated by 144 nucleotidesPf6N2E2_3939 and Pf6N2E2_3940 are separated by 37 nucleotides Pf6N2E2_3936 - Outer membrane protein assembly factor YaeT precursor, at 4,573,059 to 4,573,976 _3936 Pf6N2E2_3937 - Ribose 5-phosphate isomerase A (EC 5.3.1.6), at 4,574,142 to 4,574,813 _3937 Pf6N2E2_3938 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 4,574,951 to 4,576,465 _3938 Pf6N2E2_3939 - Predicted integral membrane protein, at 4,576,610 to 4,577,035 _3939 Pf6N2E2_3940 - Phosphoserine phosphatase (EC 3.1.3.3), at 4,577,073 to 4,577,729 _3940 Position (kb) 4574 4575 4576 4577Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 4573.951 kb on + strandat 4573.951 kb on + strandat 4573.952 kb on - strandat 4573.952 kb on - strandat 4573.952 kb on - strandat 4573.952 kb on - strandat 4573.952 kb on - strandat 4573.952 kb on - strandat 4573.974 kb on + strandat 4573.974 kb on + strandat 4573.974 kb on + strandat 4573.974 kb on + strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.975 kb on - strandat 4573.978 kb on + strandat 4573.978 kb on + strandat 4573.979 kb on - strandat 4573.979 kb on - strandat 4573.979 kb on - strandat 4573.979 kb on - strandat 4574.840 kb on - strandat 4574.856 kb on - strandat 4574.856 kb on - strandat 4574.856 kb on - strandat 4574.856 kb on - strandat 4574.856 kb on - strandat 4574.858 kb on - strandat 4574.858 kb on - strandat 4574.897 kb on - strandat 4574.897 kb on - strandat 4574.963 kb on + strandat 4574.963 kb on + strandat 4574.963 kb on + strandat 4574.964 kb on - strandat 4574.964 kb on - strandat 4574.964 kb on - strandat 4574.993 kb on + strandat 4575.116 kb on + strand, within Pf6N2E2_3938at 4575.116 kb on + strand, within Pf6N2E2_3938at 4575.116 kb on + strand, within Pf6N2E2_3938at 4575.116 kb on + strand, within Pf6N2E2_3938at 4575.116 kb on + strand, within Pf6N2E2_3938at 4575.117 kb on - strand, within Pf6N2E2_3938at 4575.117 kb on - strand, within Pf6N2E2_3938at 4575.117 kb on - strand, within Pf6N2E2_3938at 4575.117 kb on - strand, within Pf6N2E2_3938at 4575.216 kb on + strand, within Pf6N2E2_3938at 4575.217 kb on - strand, within Pf6N2E2_3938at 4575.217 kb on - strand, within Pf6N2E2_3938at 4575.217 kb on - strand, within Pf6N2E2_3938at 4575.338 kb on + strand, within Pf6N2E2_3938at 4575.339 kb on - strand, within Pf6N2E2_3938at 4575.368 kb on + strand, within Pf6N2E2_3938at 4575.368 kb on + strand, within Pf6N2E2_3938at 4575.369 kb on - strand, within Pf6N2E2_3938at 4575.369 kb on - strand, within Pf6N2E2_3938at 4575.374 kb on + strand, within Pf6N2E2_3938at 4575.374 kb on + strand, within Pf6N2E2_3938at 4575.374 kb on + strand, within Pf6N2E2_3938at 4575.374 kb on + strand, within Pf6N2E2_3938at 4575.374 kb on + strand, within Pf6N2E2_3938at 4575.374 kb on + strand, within Pf6N2E2_3938at 4575.374 kb on + strand, within Pf6N2E2_3938at 4575.375 kb on - strand, within Pf6N2E2_3938at 4575.375 kb on - strand, within Pf6N2E2_3938at 4575.375 kb on - strand, within Pf6N2E2_3938at 4575.375 kb on - strand, within Pf6N2E2_3938at 4575.375 kb on - strand, within Pf6N2E2_3938at 4575.386 kb on + strand, within Pf6N2E2_3938at 4575.516 kb on - strand, within Pf6N2E2_3938at 4575.524 kb on + strand, within Pf6N2E2_3938at 4575.525 kb on - strand, within Pf6N2E2_3938at 4575.609 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.692 kb on + strand, within Pf6N2E2_3938at 4575.693 kb on - strand, within Pf6N2E2_3938at 4575.693 kb on - strand, within Pf6N2E2_3938at 4575.693 kb on - strand, within Pf6N2E2_3938at 4575.693 kb on - strand, within Pf6N2E2_3938at 4575.693 kb on - strand, within Pf6N2E2_3938at 4575.749 kb on - strand, within Pf6N2E2_3938at 4575.749 kb on - strand, within Pf6N2E2_3938at 4575.815 kb on + strand, within Pf6N2E2_3938at 4576.025 kb on + strand, within Pf6N2E2_3938at 4576.026 kb on - strand, within Pf6N2E2_3938at 4576.032 kb on - strand, within Pf6N2E2_3938at 4576.032 kb on - strand, within Pf6N2E2_3938at 4576.032 kb on - strand, within Pf6N2E2_3938at 4576.435 kb on + strandat 4576.445 kb on + strandat 4576.445 kb on + strandat 4576.574 kb on + strandat 4576.574 kb on + strandat 4576.574 kb on + strandat 4576.574 kb on + strandat 4576.574 kb on + strandat 4576.574 kb on + strandat 4576.575 kb on - strandat 4576.575 kb on - strandat 4576.575 kb on - strandat 4576.575 kb on - strandat 4576.575 kb on - strandat 4576.575 kb on - strandat 4576.575 kb on - strandat 4576.596 kb on - strandat 4576.877 kb on + strand, within Pf6N2E2_3939at 4576.877 kb on + strand, within Pf6N2E2_3939at 4576.877 kb on + strand, within Pf6N2E2_3939at 4576.877 kb on + strand, within Pf6N2E2_3939at 4576.878 kb on - strand, within Pf6N2E2_3939at 4576.878 kb on - strand, within Pf6N2E2_3939at 4576.879 kb on + strand, within Pf6N2E2_3939at 4576.879 kb on + strand, within Pf6N2E2_3939at 4576.879 kb on + strand, within Pf6N2E2_3939at 4576.879 kb on + strand, within Pf6N2E2_3939at 4576.879 kb on + strand, within Pf6N2E2_3939at 4576.880 kb on - strand, within Pf6N2E2_3939at 4576.880 kb on - strand, within Pf6N2E2_3939at 4576.880 kb on - strand, within Pf6N2E2_3939at 4576.880 kb on - strand, within Pf6N2E2_3939at 4576.880 kb on - strand, within Pf6N2E2_3939at 4576.961 kb on - strand, within Pf6N2E2_3939at 4576.961 kb on - strand, within Pf6N2E2_3939at 4577.065 kb on + strandat 4577.201 kb on - strand, within Pf6N2E2_3940at 4577.201 kb on - strand, within Pf6N2E2_3940at 4577.206 kb on + strand, within Pf6N2E2_3940at 4577.206 kb on + strand, within Pf6N2E2_3940at 4577.206 kb on + strand, within Pf6N2E2_3940at 4577.207 kb on - strand, within Pf6N2E2_3940at 4577.207 kb on - strand, within Pf6N2E2_3940at 4577.207 kb on - strand, within Pf6N2E2_3940at 4577.207 kb on - strand, within Pf6N2E2_3940at 4577.306 kb on - strand, within Pf6N2E2_3940

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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4,573,951 + -1.3
4,573,951 + +1.2
4,573,952 - +2.0
4,573,952 - -0.3
4,573,952 - -0.8
4,573,952 - -0.5
4,573,952 - -0.5
4,573,952 - +1.6
4,573,974 + +0.4
4,573,974 + -0.8
4,573,974 + +0.0
4,573,974 + -0.3
4,573,975 - +0.4
4,573,975 - +0.6
4,573,975 - -0.8
4,573,975 - -0.1
4,573,975 - +1.6
4,573,975 - +2.0
4,573,975 - +2.0
4,573,975 - -2.1
4,573,975 - +0.5
4,573,975 - +0.6
4,573,975 - +0.7
4,573,975 - +0.5
4,573,978 + +0.5
4,573,978 + +0.0
4,573,979 - +0.0
4,573,979 - -0.3
4,573,979 - -1.3
4,573,979 - -0.0
4,574,840 - -0.6
4,574,856 - -0.2
4,574,856 - +0.1
4,574,856 - -2.2
4,574,856 - -1.2
4,574,856 - -0.9
4,574,858 - -1.3
4,574,858 - +1.0
4,574,897 - +1.0
4,574,897 - -1.2
4,574,963 + +0.9
4,574,963 + +2.0
4,574,963 + -2.4
4,574,964 - -0.7
4,574,964 - -2.4
4,574,964 - -1.4
4,574,993 + -1.5
4,575,116 + Pf6N2E2_3938 0.11 -0.5
4,575,116 + Pf6N2E2_3938 0.11 -1.0
4,575,116 + Pf6N2E2_3938 0.11 -2.3
4,575,116 + Pf6N2E2_3938 0.11 -3.2
4,575,116 + Pf6N2E2_3938 0.11 +0.6
4,575,117 - Pf6N2E2_3938 0.11 -3.3
4,575,117 - Pf6N2E2_3938 0.11 -1.3
4,575,117 - Pf6N2E2_3938 0.11 +2.0
4,575,117 - Pf6N2E2_3938 0.11 -2.9
4,575,216 + Pf6N2E2_3938 0.17 -4.0
4,575,217 - Pf6N2E2_3938 0.18 -1.0
4,575,217 - Pf6N2E2_3938 0.18 -2.3
4,575,217 - Pf6N2E2_3938 0.18 -1.5
4,575,338 + Pf6N2E2_3938 0.26 -3.2
4,575,339 - Pf6N2E2_3938 0.26 -2.1
4,575,368 + Pf6N2E2_3938 0.28 -0.7
4,575,368 + Pf6N2E2_3938 0.28 -1.4
4,575,369 - Pf6N2E2_3938 0.28 -0.8
4,575,369 - Pf6N2E2_3938 0.28 -2.9
4,575,374 + Pf6N2E2_3938 0.28 -0.6
4,575,374 + Pf6N2E2_3938 0.28 -1.7
4,575,374 + Pf6N2E2_3938 0.28 -2.0
4,575,374 + Pf6N2E2_3938 0.28 -2.1
4,575,374 + Pf6N2E2_3938 0.28 -1.0
4,575,374 + Pf6N2E2_3938 0.28 +0.0
4,575,374 + Pf6N2E2_3938 0.28 -1.0
4,575,375 - Pf6N2E2_3938 0.28 -4.0
4,575,375 - Pf6N2E2_3938 0.28 -1.2
4,575,375 - Pf6N2E2_3938 0.28 -4.9
4,575,375 - Pf6N2E2_3938 0.28 +0.0
4,575,375 - Pf6N2E2_3938 0.28 -0.0
4,575,386 + Pf6N2E2_3938 0.29 -2.0
4,575,516 - Pf6N2E2_3938 0.37 +2.0
4,575,524 + Pf6N2E2_3938 0.38 -0.3
4,575,525 - Pf6N2E2_3938 0.38 -4.5
4,575,609 + Pf6N2E2_3938 0.43 -1.8
4,575,692 + Pf6N2E2_3938 0.49 -1.3
4,575,692 + Pf6N2E2_3938 0.49 -2.4
4,575,692 + Pf6N2E2_3938 0.49 -0.8
4,575,692 + Pf6N2E2_3938 0.49 -2.5
4,575,692 + Pf6N2E2_3938 0.49 -3.6
4,575,692 + Pf6N2E2_3938 0.49 +2.0
4,575,692 + Pf6N2E2_3938 0.49 -1.5
4,575,692 + Pf6N2E2_3938 0.49 -3.5
4,575,692 + Pf6N2E2_3938 0.49 -3.4
4,575,692 + Pf6N2E2_3938 0.49 -0.5
4,575,693 - Pf6N2E2_3938 0.49 +2.0
4,575,693 - Pf6N2E2_3938 0.49 +3.0
4,575,693 - Pf6N2E2_3938 0.49 -2.3
4,575,693 - Pf6N2E2_3938 0.49 -1.6
4,575,693 - Pf6N2E2_3938 0.49 -2.5
4,575,749 - Pf6N2E2_3938 0.53 -0.6
4,575,749 - Pf6N2E2_3938 0.53 -4.3
4,575,815 + Pf6N2E2_3938 0.57 +0.1
4,576,025 + Pf6N2E2_3938 0.71 -2.6
4,576,026 - Pf6N2E2_3938 0.71 -3.5
4,576,032 - Pf6N2E2_3938 0.71 -2.0
4,576,032 - Pf6N2E2_3938 0.71 -0.5
4,576,032 - Pf6N2E2_3938 0.71 -0.5
4,576,435 + -2.7
4,576,445 + -0.9
4,576,445 + -3.9
4,576,574 + +0.4
4,576,574 + +2.0
4,576,574 + -1.3
4,576,574 + -0.1
4,576,574 + +0.2
4,576,574 + +0.1
4,576,575 - +1.0
4,576,575 - +0.5
4,576,575 - -0.2
4,576,575 - -0.9
4,576,575 - -0.7
4,576,575 - -3.6
4,576,575 - -1.0
4,576,596 - -0.8
4,576,877 + Pf6N2E2_3939 0.63 -1.3
4,576,877 + Pf6N2E2_3939 0.63 -1.0
4,576,877 + Pf6N2E2_3939 0.63 -0.5
4,576,877 + Pf6N2E2_3939 0.63 +0.4
4,576,878 - Pf6N2E2_3939 0.63 -1.3
4,576,878 - Pf6N2E2_3939 0.63 +1.4
4,576,879 + Pf6N2E2_3939 0.63 -0.7
4,576,879 + Pf6N2E2_3939 0.63 -0.0
4,576,879 + Pf6N2E2_3939 0.63 -0.3
4,576,879 + Pf6N2E2_3939 0.63 -0.6
4,576,879 + Pf6N2E2_3939 0.63 +0.5
4,576,880 - Pf6N2E2_3939 0.63 -1.8
4,576,880 - Pf6N2E2_3939 0.63 +1.0
4,576,880 - Pf6N2E2_3939 0.63 -1.1
4,576,880 - Pf6N2E2_3939 0.63 -0.1
4,576,880 - Pf6N2E2_3939 0.63 -1.5
4,576,961 - Pf6N2E2_3939 0.82 +0.5
4,576,961 - Pf6N2E2_3939 0.82 +0.0
4,577,065 + +0.1
4,577,201 - Pf6N2E2_3940 0.19 -3.1
4,577,201 - Pf6N2E2_3940 0.19 -0.5
4,577,206 + Pf6N2E2_3940 0.20 -4.0
4,577,206 + Pf6N2E2_3940 0.20 -3.7
4,577,206 + Pf6N2E2_3940 0.20 +0.3
4,577,207 - Pf6N2E2_3940 0.20 -1.4
4,577,207 - Pf6N2E2_3940 0.20 -2.5
4,577,207 - Pf6N2E2_3940 0.20 -2.5
4,577,207 - Pf6N2E2_3940 0.20 +2.0
4,577,306 - Pf6N2E2_3940 0.35 -3.0

Or see this region's nucleotide sequence