Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3198

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3196 and Pf6N2E2_3197 are separated by 63 nucleotidesPf6N2E2_3197 and Pf6N2E2_3198 are separated by 43 nucleotidesPf6N2E2_3198 and Pf6N2E2_3199 overlap by 4 nucleotides Pf6N2E2_3196 - Protein-export membrane protein SecD (TC 3.A.5.1.1), at 3,689,260 to 3,691,128 _3196 Pf6N2E2_3197 - Preprotein translocase subunit YajC (TC 3.A.5.1.1), at 3,691,192 to 3,691,530 _3197 Pf6N2E2_3198 - tRNA-guanine transglycosylase (EC 2.4.2.29), at 3,691,574 to 3,692,707 _3198 Pf6N2E2_3199 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 3,692,704 to 3,693,753 _3199 Position (kb) 3691 3692 3693Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3691.180 kb on - strandat 3691.180 kb on - strandat 3691.190 kb on - strandat 3691.190 kb on - strandat 3691.194 kb on - strandat 3691.194 kb on - strandat 3691.194 kb on - strandat 3691.194 kb on - strandat 3691.194 kb on - strandat 3691.194 kb on - strandat 3691.551 kb on - strandat 3691.612 kb on - strandat 3691.640 kb on - strandat 3691.673 kb on - strandat 3691.706 kb on - strand, within Pf6N2E2_3198at 3691.715 kb on - strand, within Pf6N2E2_3198at 3692.587 kb on - strand, within Pf6N2E2_3198at 3692.708 kb on - strandat 3692.781 kb on - strandat 3692.804 kb on + strandat 3692.804 kb on + strandat 3692.805 kb on - strandat 3692.805 kb on - strandat 3692.805 kb on - strandat 3692.926 kb on - strand, within Pf6N2E2_3199at 3693.261 kb on - strand, within Pf6N2E2_3199at 3693.307 kb on + strand, within Pf6N2E2_3199at 3693.308 kb on - strand, within Pf6N2E2_3199at 3693.309 kb on + strand, within Pf6N2E2_3199at 3693.309 kb on + strand, within Pf6N2E2_3199at 3693.309 kb on + strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.310 kb on - strand, within Pf6N2E2_3199at 3693.312 kb on - strand, within Pf6N2E2_3199at 3693.312 kb on - strand, within Pf6N2E2_3199

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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3,691,180 - -1.2
3,691,180 - -2.3
3,691,190 - -1.1
3,691,190 - -3.0
3,691,194 - +1.6
3,691,194 - -1.3
3,691,194 - +0.0
3,691,194 - +0.7
3,691,194 - -1.8
3,691,194 - +0.0
3,691,551 - -0.2
3,691,612 - -1.4
3,691,640 - +1.0
3,691,673 - +2.0
3,691,706 - Pf6N2E2_3198 0.12 +1.0
3,691,715 - Pf6N2E2_3198 0.12 -2.3
3,692,587 - Pf6N2E2_3198 0.89 -1.3
3,692,708 - +2.0
3,692,781 - +2.0
3,692,804 + +1.0
3,692,804 + +2.0
3,692,805 - +0.0
3,692,805 - +1.0
3,692,805 - +0.0
3,692,926 - Pf6N2E2_3199 0.21 +0.8
3,693,261 - Pf6N2E2_3199 0.53 -1.3
3,693,307 + Pf6N2E2_3199 0.57 -1.8
3,693,308 - Pf6N2E2_3199 0.58 +2.0
3,693,309 + Pf6N2E2_3199 0.58 -1.1
3,693,309 + Pf6N2E2_3199 0.58 +2.5
3,693,309 + Pf6N2E2_3199 0.58 +2.0
3,693,310 - Pf6N2E2_3199 0.58 +0.5
3,693,310 - Pf6N2E2_3199 0.58 +2.0
3,693,310 - Pf6N2E2_3199 0.58 +0.0
3,693,310 - Pf6N2E2_3199 0.58 -0.8
3,693,310 - Pf6N2E2_3199 0.58 -0.1
3,693,310 - Pf6N2E2_3199 0.58 -0.8
3,693,310 - Pf6N2E2_3199 0.58 +1.0
3,693,310 - Pf6N2E2_3199 0.58 -1.3
3,693,310 - Pf6N2E2_3199 0.58 +1.0
3,693,310 - Pf6N2E2_3199 0.58 +1.4
3,693,310 - Pf6N2E2_3199 0.58 +0.5
3,693,310 - Pf6N2E2_3199 0.58 -0.8
3,693,312 - Pf6N2E2_3199 0.58 +3.0
3,693,312 - Pf6N2E2_3199 0.58 +1.4

Or see this region's nucleotide sequence