Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3093 and Pf6N2E2_3094 are separated by 71 nucleotides Pf6N2E2_3094 and Pf6N2E2_3095 overlap by 4 nucleotides Pf6N2E2_3095 and Pf6N2E2_3096 overlap by 22 nucleotides Pf6N2E2_3096 and Pf6N2E2_3097 are separated by 1 nucleotides Pf6N2E2_3097 and Pf6N2E2_3098 are separated by 16 nucleotides
Pf6N2E2_3093 - Transcriptional regulator, TetR family, at 3,581,921 to 3,582,556
_3093
Pf6N2E2_3094 - SAM-dependent methyltransferase (EC 2.1.1.-), at 3,582,628 to 3,583,410
_3094
Pf6N2E2_3095 - hypothetical protein, at 3,583,407 to 3,583,523
_3095
Pf6N2E2_3096 - Periplasmic protein TonB, links inner and outer membranes, at 3,583,502 to 3,584,122
_3096
Pf6N2E2_3097 - extensin-like protein, at 3,584,124 to 3,584,822
_3097
Pf6N2E2_3098 - Probable carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 3,584,839 to 3,585,660
_3098
Position (kb)
3583
3584
3585 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.514 kb on - strand at 3582.514 kb on - strand at 3582.514 kb on - strand at 3582.554 kb on + strand at 3582.554 kb on + strand at 3582.555 kb on - strand at 3582.653 kb on + strand at 3582.662 kb on + strand at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.881 kb on + strand, within Pf6N2E2_3094 at 3582.881 kb on + strand, within Pf6N2E2_3094 at 3582.882 kb on - strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.179 kb on - strand, within Pf6N2E2_3094 at 3583.431 kb on - strand, within Pf6N2E2_3095 at 3583.553 kb on + strand at 3583.553 kb on + strand at 3583.554 kb on - strand at 3583.554 kb on - strand at 3583.665 kb on - strand, within Pf6N2E2_3096 at 3584.185 kb on + strand at 3584.186 kb on - strand at 3584.186 kb on - strand at 3584.186 kb on - strand at 3584.186 kb on - strand at 3584.214 kb on - strand, within Pf6N2E2_3097 at 3584.214 kb on - strand, within Pf6N2E2_3097 at 3584.214 kb on - strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.434 kb on + strand, within Pf6N2E2_3097 at 3584.434 kb on + strand, within Pf6N2E2_3097 at 3584.434 kb on + strand, within Pf6N2E2_3097 at 3584.435 kb on - strand, within Pf6N2E2_3097 at 3584.435 kb on - strand, within Pf6N2E2_3097 at 3584.576 kb on + strand, within Pf6N2E2_3097 at 3584.577 kb on - strand, within Pf6N2E2_3097 at 3584.577 kb on - strand, within Pf6N2E2_3097 at 3584.852 kb on - strand at 3584.852 kb on - strand at 3584.852 kb on - strand at 3584.852 kb on - strand at 3584.945 kb on + strand, within Pf6N2E2_3098 at 3585.114 kb on - strand, within Pf6N2E2_3098
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 3,582,513 + -0.1 3,582,513 + +2.0 3,582,513 + +1.4 3,582,513 + +1.0 3,582,513 + -0.3 3,582,514 - +0.7 3,582,514 - +2.3 3,582,514 - +0.9 3,582,554 + -0.5 3,582,554 + -1.6 3,582,555 - +0.4 3,582,653 + +0.0 3,582,662 + +0.0 3,582,711 + Pf6N2E2_3094 0.11 -0.8 3,582,711 + Pf6N2E2_3094 0.11 +1.0 3,582,711 + Pf6N2E2_3094 0.11 -3.4 3,582,711 + Pf6N2E2_3094 0.11 -3.1 3,582,711 + Pf6N2E2_3094 0.11 +0.0 3,582,711 + Pf6N2E2_3094 0.11 +2.0 3,582,712 - Pf6N2E2_3094 0.11 -1.2 3,582,712 - Pf6N2E2_3094 0.11 +2.0 3,582,712 - Pf6N2E2_3094 0.11 -0.2 3,582,881 + Pf6N2E2_3094 0.32 +1.0 3,582,881 + Pf6N2E2_3094 0.32 -0.6 3,582,882 - Pf6N2E2_3094 0.32 +2.0 3,583,178 + Pf6N2E2_3094 0.70 -0.6 3,583,178 + Pf6N2E2_3094 0.70 -2.1 3,583,178 + Pf6N2E2_3094 0.70 +2.0 3,583,179 - Pf6N2E2_3094 0.70 -1.6 3,583,431 - Pf6N2E2_3095 0.21 +0.8 3,583,553 + +1.0 3,583,553 + +2.0 3,583,554 - -0.8 3,583,554 - +0.4 3,583,665 - Pf6N2E2_3096 0.26 -1.8 3,584,185 + +0.4 3,584,186 - +0.6 3,584,186 - -0.4 3,584,186 - -3.2 3,584,186 - +0.1 3,584,214 - Pf6N2E2_3097 0.13 -0.4 3,584,214 - Pf6N2E2_3097 0.13 +0.0 3,584,214 - Pf6N2E2_3097 0.13 +1.2 3,584,377 + Pf6N2E2_3097 0.36 +0.9 3,584,377 + Pf6N2E2_3097 0.36 +0.4 3,584,377 + Pf6N2E2_3097 0.36 -0.7 3,584,377 + Pf6N2E2_3097 0.36 +0.4 3,584,378 - Pf6N2E2_3097 0.36 +1.0 3,584,378 - Pf6N2E2_3097 0.36 +0.0 3,584,378 - Pf6N2E2_3097 0.36 +0.9 3,584,378 - Pf6N2E2_3097 0.36 -1.0 3,584,378 - Pf6N2E2_3097 0.36 -1.6 3,584,378 - Pf6N2E2_3097 0.36 +2.0 3,584,378 - Pf6N2E2_3097 0.36 +2.3 3,584,378 - Pf6N2E2_3097 0.36 +1.3 3,584,434 + Pf6N2E2_3097 0.44 -1.0 3,584,434 + Pf6N2E2_3097 0.44 -0.2 3,584,434 + Pf6N2E2_3097 0.44 +0.3 3,584,435 - Pf6N2E2_3097 0.44 -1.3 3,584,435 - Pf6N2E2_3097 0.44 -0.2 3,584,576 + Pf6N2E2_3097 0.65 -0.4 3,584,577 - Pf6N2E2_3097 0.65 -1.4 3,584,577 - Pf6N2E2_3097 0.65 +0.6 3,584,852 - -0.3 3,584,852 - +0.4 3,584,852 - -0.3 3,584,852 - -1.9 3,584,945 + Pf6N2E2_3098 0.13 -1.1 3,585,114 - Pf6N2E2_3098 0.33 +0.9
Or see this region's nucleotide sequence