Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3096

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3093 and Pf6N2E2_3094 are separated by 71 nucleotidesPf6N2E2_3094 and Pf6N2E2_3095 overlap by 4 nucleotidesPf6N2E2_3095 and Pf6N2E2_3096 overlap by 22 nucleotidesPf6N2E2_3096 and Pf6N2E2_3097 are separated by 1 nucleotidesPf6N2E2_3097 and Pf6N2E2_3098 are separated by 16 nucleotides Pf6N2E2_3093 - Transcriptional regulator, TetR family, at 3,581,921 to 3,582,556 _3093 Pf6N2E2_3094 - SAM-dependent methyltransferase (EC 2.1.1.-), at 3,582,628 to 3,583,410 _3094 Pf6N2E2_3095 - hypothetical protein, at 3,583,407 to 3,583,523 _3095 Pf6N2E2_3096 - Periplasmic protein TonB, links inner and outer membranes, at 3,583,502 to 3,584,122 _3096 Pf6N2E2_3097 - extensin-like protein, at 3,584,124 to 3,584,822 _3097 Pf6N2E2_3098 - Probable carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 3,584,839 to 3,585,660 _3098 Position (kb) 3583 3584 3585Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3582.513 kb on + strandat 3582.513 kb on + strandat 3582.513 kb on + strandat 3582.513 kb on + strandat 3582.513 kb on + strandat 3582.514 kb on - strandat 3582.514 kb on - strandat 3582.514 kb on - strandat 3582.554 kb on + strandat 3582.554 kb on + strandat 3582.555 kb on - strandat 3582.653 kb on + strandat 3582.662 kb on + strandat 3582.711 kb on + strand, within Pf6N2E2_3094at 3582.711 kb on + strand, within Pf6N2E2_3094at 3582.711 kb on + strand, within Pf6N2E2_3094at 3582.711 kb on + strand, within Pf6N2E2_3094at 3582.711 kb on + strand, within Pf6N2E2_3094at 3582.711 kb on + strand, within Pf6N2E2_3094at 3582.712 kb on - strand, within Pf6N2E2_3094at 3582.712 kb on - strand, within Pf6N2E2_3094at 3582.712 kb on - strand, within Pf6N2E2_3094at 3582.881 kb on + strand, within Pf6N2E2_3094at 3582.881 kb on + strand, within Pf6N2E2_3094at 3582.882 kb on - strand, within Pf6N2E2_3094at 3583.178 kb on + strand, within Pf6N2E2_3094at 3583.178 kb on + strand, within Pf6N2E2_3094at 3583.178 kb on + strand, within Pf6N2E2_3094at 3583.179 kb on - strand, within Pf6N2E2_3094at 3583.431 kb on - strand, within Pf6N2E2_3095at 3583.553 kb on + strandat 3583.553 kb on + strandat 3583.554 kb on - strandat 3583.554 kb on - strandat 3583.665 kb on - strand, within Pf6N2E2_3096at 3584.185 kb on + strandat 3584.186 kb on - strandat 3584.186 kb on - strandat 3584.186 kb on - strandat 3584.186 kb on - strandat 3584.214 kb on - strand, within Pf6N2E2_3097at 3584.214 kb on - strand, within Pf6N2E2_3097at 3584.214 kb on - strand, within Pf6N2E2_3097at 3584.377 kb on + strand, within Pf6N2E2_3097at 3584.377 kb on + strand, within Pf6N2E2_3097at 3584.377 kb on + strand, within Pf6N2E2_3097at 3584.377 kb on + strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.378 kb on - strand, within Pf6N2E2_3097at 3584.434 kb on + strand, within Pf6N2E2_3097at 3584.434 kb on + strand, within Pf6N2E2_3097at 3584.434 kb on + strand, within Pf6N2E2_3097at 3584.435 kb on - strand, within Pf6N2E2_3097at 3584.435 kb on - strand, within Pf6N2E2_3097at 3584.576 kb on + strand, within Pf6N2E2_3097at 3584.577 kb on - strand, within Pf6N2E2_3097at 3584.577 kb on - strand, within Pf6N2E2_3097at 3584.852 kb on - strandat 3584.852 kb on - strandat 3584.852 kb on - strandat 3584.852 kb on - strandat 3584.945 kb on + strand, within Pf6N2E2_3098at 3585.114 kb on - strand, within Pf6N2E2_3098

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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3,582,513 + -0.1
3,582,513 + +2.0
3,582,513 + +1.4
3,582,513 + +1.0
3,582,513 + -0.3
3,582,514 - +0.7
3,582,514 - +2.3
3,582,514 - +0.9
3,582,554 + -0.5
3,582,554 + -1.6
3,582,555 - +0.4
3,582,653 + +0.0
3,582,662 + +0.0
3,582,711 + Pf6N2E2_3094 0.11 -0.8
3,582,711 + Pf6N2E2_3094 0.11 +1.0
3,582,711 + Pf6N2E2_3094 0.11 -3.4
3,582,711 + Pf6N2E2_3094 0.11 -3.1
3,582,711 + Pf6N2E2_3094 0.11 +0.0
3,582,711 + Pf6N2E2_3094 0.11 +2.0
3,582,712 - Pf6N2E2_3094 0.11 -1.2
3,582,712 - Pf6N2E2_3094 0.11 +2.0
3,582,712 - Pf6N2E2_3094 0.11 -0.2
3,582,881 + Pf6N2E2_3094 0.32 +1.0
3,582,881 + Pf6N2E2_3094 0.32 -0.6
3,582,882 - Pf6N2E2_3094 0.32 +2.0
3,583,178 + Pf6N2E2_3094 0.70 -0.6
3,583,178 + Pf6N2E2_3094 0.70 -2.1
3,583,178 + Pf6N2E2_3094 0.70 +2.0
3,583,179 - Pf6N2E2_3094 0.70 -1.6
3,583,431 - Pf6N2E2_3095 0.21 +0.8
3,583,553 + +1.0
3,583,553 + +2.0
3,583,554 - -0.8
3,583,554 - +0.4
3,583,665 - Pf6N2E2_3096 0.26 -1.8
3,584,185 + +0.4
3,584,186 - +0.6
3,584,186 - -0.4
3,584,186 - -3.2
3,584,186 - +0.1
3,584,214 - Pf6N2E2_3097 0.13 -0.4
3,584,214 - Pf6N2E2_3097 0.13 +0.0
3,584,214 - Pf6N2E2_3097 0.13 +1.2
3,584,377 + Pf6N2E2_3097 0.36 +0.9
3,584,377 + Pf6N2E2_3097 0.36 +0.4
3,584,377 + Pf6N2E2_3097 0.36 -0.7
3,584,377 + Pf6N2E2_3097 0.36 +0.4
3,584,378 - Pf6N2E2_3097 0.36 +1.0
3,584,378 - Pf6N2E2_3097 0.36 +0.0
3,584,378 - Pf6N2E2_3097 0.36 +0.9
3,584,378 - Pf6N2E2_3097 0.36 -1.0
3,584,378 - Pf6N2E2_3097 0.36 -1.6
3,584,378 - Pf6N2E2_3097 0.36 +2.0
3,584,378 - Pf6N2E2_3097 0.36 +2.3
3,584,378 - Pf6N2E2_3097 0.36 +1.3
3,584,434 + Pf6N2E2_3097 0.44 -1.0
3,584,434 + Pf6N2E2_3097 0.44 -0.2
3,584,434 + Pf6N2E2_3097 0.44 +0.3
3,584,435 - Pf6N2E2_3097 0.44 -1.3
3,584,435 - Pf6N2E2_3097 0.44 -0.2
3,584,576 + Pf6N2E2_3097 0.65 -0.4
3,584,577 - Pf6N2E2_3097 0.65 -1.4
3,584,577 - Pf6N2E2_3097 0.65 +0.6
3,584,852 - -0.3
3,584,852 - +0.4
3,584,852 - -0.3
3,584,852 - -1.9
3,584,945 + Pf6N2E2_3098 0.13 -1.1
3,585,114 - Pf6N2E2_3098 0.33 +0.9

Or see this region's nucleotide sequence