Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2734 and Pf6N2E2_2735 are separated by 227 nucleotides Pf6N2E2_2735 and Pf6N2E2_2736 overlap by 4 nucleotides Pf6N2E2_2736 and Pf6N2E2_2737 overlap by 4 nucleotides
Pf6N2E2_2734 - Transcriptional regulator, LysR family, at 3,225,392 to 3,226,315
_2734
Pf6N2E2_2735 - hypothetical protein, at 3,226,543 to 3,228,630
_2735
Pf6N2E2_2736 - hypothetical protein, at 3,228,627 to 3,229,010
_2736
Pf6N2E2_2737 - SAM-dependent methyltransferases, at 3,229,007 to 3,229,717
_2737
Position (kb)
3226
3227
3228
3229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3225.572 kb on - strand, within Pf6N2E2_2734 at 3225.573 kb on + strand, within Pf6N2E2_2734 at 3225.574 kb on - strand, within Pf6N2E2_2734 at 3225.744 kb on + strand, within Pf6N2E2_2734 at 3225.745 kb on - strand, within Pf6N2E2_2734 at 3226.349 kb on + strand at 3226.349 kb on + strand at 3226.349 kb on + strand at 3226.349 kb on + strand at 3226.350 kb on - strand at 3226.373 kb on + strand at 3226.403 kb on + strand at 3226.403 kb on + strand at 3226.403 kb on + strand at 3226.403 kb on + strand at 3226.545 kb on - strand at 3226.782 kb on + strand, within Pf6N2E2_2735 at 3226.783 kb on - strand, within Pf6N2E2_2735 at 3227.120 kb on + strand, within Pf6N2E2_2735 at 3227.121 kb on - strand, within Pf6N2E2_2735 at 3227.121 kb on - strand, within Pf6N2E2_2735 at 3227.121 kb on - strand, within Pf6N2E2_2735 at 3227.138 kb on + strand, within Pf6N2E2_2735 at 3227.138 kb on + strand, within Pf6N2E2_2735 at 3227.139 kb on - strand, within Pf6N2E2_2735 at 3227.213 kb on + strand, within Pf6N2E2_2735 at 3227.214 kb on - strand, within Pf6N2E2_2735 at 3227.214 kb on - strand, within Pf6N2E2_2735 at 3227.249 kb on + strand, within Pf6N2E2_2735 at 3227.249 kb on + strand, within Pf6N2E2_2735 at 3227.313 kb on - strand, within Pf6N2E2_2735 at 3227.509 kb on + strand, within Pf6N2E2_2735 at 3227.509 kb on + strand, within Pf6N2E2_2735 at 3227.509 kb on + strand, within Pf6N2E2_2735 at 3227.509 kb on + strand, within Pf6N2E2_2735 at 3227.510 kb on - strand, within Pf6N2E2_2735 at 3227.519 kb on + strand, within Pf6N2E2_2735 at 3227.520 kb on - strand, within Pf6N2E2_2735 at 3227.520 kb on - strand, within Pf6N2E2_2735 at 3227.520 kb on - strand, within Pf6N2E2_2735 at 3227.520 kb on - strand, within Pf6N2E2_2735 at 3227.594 kb on + strand, within Pf6N2E2_2735 at 3227.595 kb on - strand, within Pf6N2E2_2735 at 3227.595 kb on - strand, within Pf6N2E2_2735 at 3227.768 kb on + strand, within Pf6N2E2_2735 at 3227.768 kb on + strand, within Pf6N2E2_2735 at 3227.768 kb on + strand, within Pf6N2E2_2735 at 3227.769 kb on - strand, within Pf6N2E2_2735 at 3227.769 kb on - strand, within Pf6N2E2_2735 at 3227.769 kb on - strand, within Pf6N2E2_2735 at 3227.783 kb on + strand, within Pf6N2E2_2735 at 3227.784 kb on - strand, within Pf6N2E2_2735 at 3227.819 kb on + strand, within Pf6N2E2_2735 at 3227.819 kb on + strand, within Pf6N2E2_2735 at 3227.820 kb on - strand, within Pf6N2E2_2735 at 3227.820 kb on - strand, within Pf6N2E2_2735 at 3227.820 kb on - strand, within Pf6N2E2_2735 at 3227.820 kb on - strand, within Pf6N2E2_2735 at 3227.851 kb on + strand, within Pf6N2E2_2735 at 3227.851 kb on + strand, within Pf6N2E2_2735 at 3227.852 kb on - strand, within Pf6N2E2_2735 at 3227.852 kb on - strand, within Pf6N2E2_2735 at 3228.050 kb on + strand, within Pf6N2E2_2735 at 3228.051 kb on - strand, within Pf6N2E2_2735 at 3228.079 kb on + strand, within Pf6N2E2_2735 at 3228.131 kb on + strand, within Pf6N2E2_2735 at 3228.132 kb on - strand, within Pf6N2E2_2735 at 3228.132 kb on - strand, within Pf6N2E2_2735 at 3228.132 kb on - strand, within Pf6N2E2_2735 at 3228.425 kb on + strand at 3228.426 kb on - strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.489 kb on - strand at 3228.489 kb on - strand at 3228.489 kb on - strand at 3228.707 kb on - strand, within Pf6N2E2_2736 at 3228.825 kb on + strand, within Pf6N2E2_2736 at 3228.825 kb on + strand, within Pf6N2E2_2736 at 3228.826 kb on - strand, within Pf6N2E2_2736 at 3228.939 kb on + strand, within Pf6N2E2_2736 at 3228.940 kb on - strand, within Pf6N2E2_2736 at 3228.940 kb on - strand, within Pf6N2E2_2736 at 3229.049 kb on - strand at 3229.196 kb on + strand, within Pf6N2E2_2737 at 3229.197 kb on - strand, within Pf6N2E2_2737 at 3229.197 kb on - strand, within Pf6N2E2_2737 at 3229.210 kb on - strand, within Pf6N2E2_2737 at 3229.210 kb on - strand, within Pf6N2E2_2737 at 3229.212 kb on + strand, within Pf6N2E2_2737 at 3229.212 kb on + strand, within Pf6N2E2_2737 at 3229.213 kb on - strand, within Pf6N2E2_2737 at 3229.275 kb on + strand, within Pf6N2E2_2737 at 3229.276 kb on - strand, within Pf6N2E2_2737 at 3229.276 kb on - strand, within Pf6N2E2_2737 at 3229.276 kb on - strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.378 kb on - strand, within Pf6N2E2_2737 at 3229.609 kb on + strand, within Pf6N2E2_2737
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 3,225,572 - Pf6N2E2_2734 0.19 +0.3 3,225,573 + Pf6N2E2_2734 0.20 +0.8 3,225,574 - Pf6N2E2_2734 0.20 +0.2 3,225,744 + Pf6N2E2_2734 0.38 -1.4 3,225,745 - Pf6N2E2_2734 0.38 -1.0 3,226,349 + -2.6 3,226,349 + -0.6 3,226,349 + +0.9 3,226,349 + -2.6 3,226,350 - -2.1 3,226,373 + +0.4 3,226,403 + -0.9 3,226,403 + -0.6 3,226,403 + +0.4 3,226,403 + -0.8 3,226,545 - -0.6 3,226,782 + Pf6N2E2_2735 0.11 +0.3 3,226,783 - Pf6N2E2_2735 0.11 -0.6 3,227,120 + Pf6N2E2_2735 0.28 -1.7 3,227,121 - Pf6N2E2_2735 0.28 -0.4 3,227,121 - Pf6N2E2_2735 0.28 +0.2 3,227,121 - Pf6N2E2_2735 0.28 +0.3 3,227,138 + Pf6N2E2_2735 0.28 +0.4 3,227,138 + Pf6N2E2_2735 0.28 -0.1 3,227,139 - Pf6N2E2_2735 0.29 -0.2 3,227,213 + Pf6N2E2_2735 0.32 +0.7 3,227,214 - Pf6N2E2_2735 0.32 -0.4 3,227,214 - Pf6N2E2_2735 0.32 -0.7 3,227,249 + Pf6N2E2_2735 0.34 -2.0 3,227,249 + Pf6N2E2_2735 0.34 +0.1 3,227,313 - Pf6N2E2_2735 0.37 -0.5 3,227,509 + Pf6N2E2_2735 0.46 +0.9 3,227,509 + Pf6N2E2_2735 0.46 +0.3 3,227,509 + Pf6N2E2_2735 0.46 -3.0 3,227,509 + Pf6N2E2_2735 0.46 -1.1 3,227,510 - Pf6N2E2_2735 0.46 -2.8 3,227,519 + Pf6N2E2_2735 0.47 -1.8 3,227,520 - Pf6N2E2_2735 0.47 -0.1 3,227,520 - Pf6N2E2_2735 0.47 +1.9 3,227,520 - Pf6N2E2_2735 0.47 -1.5 3,227,520 - Pf6N2E2_2735 0.47 +0.1 3,227,594 + Pf6N2E2_2735 0.50 +1.9 3,227,595 - Pf6N2E2_2735 0.50 +0.9 3,227,595 - Pf6N2E2_2735 0.50 -1.1 3,227,768 + Pf6N2E2_2735 0.59 -2.9 3,227,768 + Pf6N2E2_2735 0.59 -0.0 3,227,768 + Pf6N2E2_2735 0.59 -1.2 3,227,769 - Pf6N2E2_2735 0.59 -1.1 3,227,769 - Pf6N2E2_2735 0.59 -0.7 3,227,769 - Pf6N2E2_2735 0.59 +1.9 3,227,783 + Pf6N2E2_2735 0.59 -0.7 3,227,784 - Pf6N2E2_2735 0.59 -1.4 3,227,819 + Pf6N2E2_2735 0.61 +0.2 3,227,819 + Pf6N2E2_2735 0.61 -0.4 3,227,820 - Pf6N2E2_2735 0.61 +2.0 3,227,820 - Pf6N2E2_2735 0.61 +0.9 3,227,820 - Pf6N2E2_2735 0.61 -3.5 3,227,820 - Pf6N2E2_2735 0.61 -0.5 3,227,851 + Pf6N2E2_2735 0.63 +1.9 3,227,851 + Pf6N2E2_2735 0.63 -2.8 3,227,852 - Pf6N2E2_2735 0.63 +1.0 3,227,852 - Pf6N2E2_2735 0.63 -0.1 3,228,050 + Pf6N2E2_2735 0.72 -1.1 3,228,051 - Pf6N2E2_2735 0.72 -1.0 3,228,079 + Pf6N2E2_2735 0.74 -1.2 3,228,131 + Pf6N2E2_2735 0.76 -2.8 3,228,132 - Pf6N2E2_2735 0.76 +1.9 3,228,132 - Pf6N2E2_2735 0.76 +0.9 3,228,132 - Pf6N2E2_2735 0.76 -0.4 3,228,425 + -2.9 3,228,426 - +0.3 3,228,488 + +0.3 3,228,488 + -0.2 3,228,488 + -0.7 3,228,488 + -0.8 3,228,488 + -0.8 3,228,489 - -0.7 3,228,489 - -0.2 3,228,489 - +1.9 3,228,707 - Pf6N2E2_2736 0.21 +0.3 3,228,825 + Pf6N2E2_2736 0.52 +0.2 3,228,825 + Pf6N2E2_2736 0.52 +0.7 3,228,826 - Pf6N2E2_2736 0.52 -1.2 3,228,939 + Pf6N2E2_2736 0.81 +0.0 3,228,940 - Pf6N2E2_2736 0.82 -1.2 3,228,940 - Pf6N2E2_2736 0.82 -0.5 3,229,049 - -0.8 3,229,196 + Pf6N2E2_2737 0.27 +1.5 3,229,197 - Pf6N2E2_2737 0.27 +1.9 3,229,197 - Pf6N2E2_2737 0.27 -0.2 3,229,210 - Pf6N2E2_2737 0.29 -0.7 3,229,210 - Pf6N2E2_2737 0.29 +1.9 3,229,212 + Pf6N2E2_2737 0.29 -0.0 3,229,212 + Pf6N2E2_2737 0.29 -0.1 3,229,213 - Pf6N2E2_2737 0.29 -0.0 3,229,275 + Pf6N2E2_2737 0.38 +0.3 3,229,276 - Pf6N2E2_2737 0.38 -1.5 3,229,276 - Pf6N2E2_2737 0.38 +0.6 3,229,276 - Pf6N2E2_2737 0.38 -0.5 3,229,377 + Pf6N2E2_2737 0.52 +0.5 3,229,377 + Pf6N2E2_2737 0.52 -0.2 3,229,377 + Pf6N2E2_2737 0.52 -0.7 3,229,377 + Pf6N2E2_2737 0.52 -0.7 3,229,378 - Pf6N2E2_2737 0.52 -0.7 3,229,609 + Pf6N2E2_2737 0.85 -0.7
Or see this region's nucleotide sequence