Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2687 and Pf6N2E2_2688 are separated by 180 nucleotides Pf6N2E2_2688 and Pf6N2E2_2689 are separated by 1 nucleotides
Pf6N2E2_2687 - COGs COG3146, at 3,175,977 to 3,177,101
_2687
Pf6N2E2_2688 - YD repeat protein, at 3,177,282 to 3,178,313
_2688
Pf6N2E2_2689 - YD repeat protein, at 3,178,315 to 3,179,391
_2689
Position (kb)
3177
3178
3179 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3176.392 kb on + strand, within Pf6N2E2_2687 at 3176.416 kb on + strand, within Pf6N2E2_2687 at 3176.416 kb on + strand, within Pf6N2E2_2687 at 3176.416 kb on + strand, within Pf6N2E2_2687 at 3176.416 kb on + strand, within Pf6N2E2_2687 at 3176.416 kb on + strand, within Pf6N2E2_2687 at 3176.417 kb on - strand, within Pf6N2E2_2687 at 3176.417 kb on - strand, within Pf6N2E2_2687 at 3176.428 kb on + strand, within Pf6N2E2_2687 at 3176.429 kb on - strand, within Pf6N2E2_2687 at 3176.438 kb on - strand, within Pf6N2E2_2687 at 3176.606 kb on - strand, within Pf6N2E2_2687 at 3176.714 kb on - strand, within Pf6N2E2_2687 at 3176.714 kb on - strand, within Pf6N2E2_2687 at 3176.821 kb on + strand, within Pf6N2E2_2687 at 3176.822 kb on - strand, within Pf6N2E2_2687 at 3176.872 kb on + strand, within Pf6N2E2_2687 at 3176.872 kb on + strand, within Pf6N2E2_2687 at 3176.872 kb on + strand, within Pf6N2E2_2687 at 3176.873 kb on - strand, within Pf6N2E2_2687 at 3177.174 kb on + strand at 3177.174 kb on + strand at 3177.212 kb on - strand at 3177.229 kb on - strand at 3177.229 kb on - strand at 3177.707 kb on + strand, within Pf6N2E2_2688 at 3177.794 kb on + strand, within Pf6N2E2_2688 at 3177.815 kb on - strand, within Pf6N2E2_2688 at 3177.821 kb on - strand, within Pf6N2E2_2688 at 3177.821 kb on - strand, within Pf6N2E2_2688 at 3178.037 kb on + strand, within Pf6N2E2_2688 at 3178.192 kb on + strand, within Pf6N2E2_2688 at 3178.253 kb on - strand at 3178.316 kb on + strand at 3178.316 kb on + strand at 3178.415 kb on - strand at 3178.417 kb on - strand at 3178.417 kb on - strand at 3178.570 kb on - strand, within Pf6N2E2_2689 at 3178.629 kb on - strand, within Pf6N2E2_2689 at 3178.871 kb on + strand, within Pf6N2E2_2689 at 3178.872 kb on - strand, within Pf6N2E2_2689 at 3178.872 kb on - strand, within Pf6N2E2_2689 at 3178.878 kb on - strand, within Pf6N2E2_2689 at 3179.026 kb on - strand, within Pf6N2E2_2689 at 3179.118 kb on + strand, within Pf6N2E2_2689 at 3179.137 kb on + strand, within Pf6N2E2_2689 at 3179.277 kb on + strand, within Pf6N2E2_2689 at 3179.278 kb on - strand, within Pf6N2E2_2689 at 3179.309 kb on + strand at 3179.310 kb on - strand at 3179.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 3,176,392 + Pf6N2E2_2687 0.37 -0.7 3,176,416 + Pf6N2E2_2687 0.39 +0.1 3,176,416 + Pf6N2E2_2687 0.39 +0.3 3,176,416 + Pf6N2E2_2687 0.39 -0.3 3,176,416 + Pf6N2E2_2687 0.39 +1.9 3,176,416 + Pf6N2E2_2687 0.39 +0.0 3,176,417 - Pf6N2E2_2687 0.39 +0.4 3,176,417 - Pf6N2E2_2687 0.39 +0.5 3,176,428 + Pf6N2E2_2687 0.40 -0.4 3,176,429 - Pf6N2E2_2687 0.40 -1.1 3,176,438 - Pf6N2E2_2687 0.41 +0.8 3,176,606 - Pf6N2E2_2687 0.56 +0.7 3,176,714 - Pf6N2E2_2687 0.66 -1.1 3,176,714 - Pf6N2E2_2687 0.66 -1.7 3,176,821 + Pf6N2E2_2687 0.75 +1.9 3,176,822 - Pf6N2E2_2687 0.75 -0.0 3,176,872 + Pf6N2E2_2687 0.80 -1.1 3,176,872 + Pf6N2E2_2687 0.80 +0.3 3,176,872 + Pf6N2E2_2687 0.80 -1.9 3,176,873 - Pf6N2E2_2687 0.80 -0.2 3,177,174 + -1.4 3,177,174 + +0.9 3,177,212 - +1.9 3,177,229 - -2.3 3,177,229 - -1.2 3,177,707 + Pf6N2E2_2688 0.41 +0.3 3,177,794 + Pf6N2E2_2688 0.50 +1.9 3,177,815 - Pf6N2E2_2688 0.52 -2.2 3,177,821 - Pf6N2E2_2688 0.52 -0.4 3,177,821 - Pf6N2E2_2688 0.52 +0.3 3,178,037 + Pf6N2E2_2688 0.73 +1.9 3,178,192 + Pf6N2E2_2688 0.88 -2.5 3,178,253 - +0.4 3,178,316 + +0.2 3,178,316 + -0.1 3,178,415 - +0.1 3,178,417 - +0.3 3,178,417 - +1.9 3,178,570 - Pf6N2E2_2689 0.24 +0.8 3,178,629 - Pf6N2E2_2689 0.29 +1.2 3,178,871 + Pf6N2E2_2689 0.52 +0.7 3,178,872 - Pf6N2E2_2689 0.52 +1.1 3,178,872 - Pf6N2E2_2689 0.52 +0.3 3,178,878 - Pf6N2E2_2689 0.52 +1.9 3,179,026 - Pf6N2E2_2689 0.66 +0.4 3,179,118 + Pf6N2E2_2689 0.75 -0.1 3,179,137 + Pf6N2E2_2689 0.76 -0.3 3,179,277 + Pf6N2E2_2689 0.89 +0.9 3,179,278 - Pf6N2E2_2689 0.89 -0.8 3,179,309 + -0.4 3,179,310 - -1.3 3,179,310 - +0.9
Or see this region's nucleotide sequence