Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2688

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2687 and Pf6N2E2_2688 are separated by 180 nucleotidesPf6N2E2_2688 and Pf6N2E2_2689 are separated by 1 nucleotides Pf6N2E2_2687 - COGs COG3146, at 3,175,977 to 3,177,101 _2687 Pf6N2E2_2688 - YD repeat protein, at 3,177,282 to 3,178,313 _2688 Pf6N2E2_2689 - YD repeat protein, at 3,178,315 to 3,179,391 _2689 Position (kb) 3177 3178 3179Strain fitness (log2 ratio) -2 -1 0 1 2at 3176.392 kb on + strand, within Pf6N2E2_2687at 3176.416 kb on + strand, within Pf6N2E2_2687at 3176.416 kb on + strand, within Pf6N2E2_2687at 3176.416 kb on + strand, within Pf6N2E2_2687at 3176.416 kb on + strand, within Pf6N2E2_2687at 3176.416 kb on + strand, within Pf6N2E2_2687at 3176.417 kb on - strand, within Pf6N2E2_2687at 3176.417 kb on - strand, within Pf6N2E2_2687at 3176.428 kb on + strand, within Pf6N2E2_2687at 3176.429 kb on - strand, within Pf6N2E2_2687at 3176.438 kb on - strand, within Pf6N2E2_2687at 3176.606 kb on - strand, within Pf6N2E2_2687at 3176.714 kb on - strand, within Pf6N2E2_2687at 3176.714 kb on - strand, within Pf6N2E2_2687at 3176.821 kb on + strand, within Pf6N2E2_2687at 3176.822 kb on - strand, within Pf6N2E2_2687at 3176.872 kb on + strand, within Pf6N2E2_2687at 3176.872 kb on + strand, within Pf6N2E2_2687at 3176.872 kb on + strand, within Pf6N2E2_2687at 3176.873 kb on - strand, within Pf6N2E2_2687at 3177.174 kb on + strandat 3177.174 kb on + strandat 3177.212 kb on - strandat 3177.229 kb on - strandat 3177.229 kb on - strandat 3177.707 kb on + strand, within Pf6N2E2_2688at 3177.794 kb on + strand, within Pf6N2E2_2688at 3177.815 kb on - strand, within Pf6N2E2_2688at 3177.821 kb on - strand, within Pf6N2E2_2688at 3177.821 kb on - strand, within Pf6N2E2_2688at 3178.037 kb on + strand, within Pf6N2E2_2688at 3178.192 kb on + strand, within Pf6N2E2_2688at 3178.253 kb on - strandat 3178.316 kb on + strandat 3178.316 kb on + strandat 3178.415 kb on - strandat 3178.417 kb on - strandat 3178.417 kb on - strandat 3178.570 kb on - strand, within Pf6N2E2_2689at 3178.629 kb on - strand, within Pf6N2E2_2689at 3178.871 kb on + strand, within Pf6N2E2_2689at 3178.872 kb on - strand, within Pf6N2E2_2689at 3178.872 kb on - strand, within Pf6N2E2_2689at 3178.878 kb on - strand, within Pf6N2E2_2689at 3179.026 kb on - strand, within Pf6N2E2_2689at 3179.118 kb on + strand, within Pf6N2E2_2689at 3179.137 kb on + strand, within Pf6N2E2_2689at 3179.277 kb on + strand, within Pf6N2E2_2689at 3179.278 kb on - strand, within Pf6N2E2_2689at 3179.309 kb on + strandat 3179.310 kb on - strandat 3179.310 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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3,176,392 + Pf6N2E2_2687 0.37 -0.7
3,176,416 + Pf6N2E2_2687 0.39 +0.1
3,176,416 + Pf6N2E2_2687 0.39 +0.3
3,176,416 + Pf6N2E2_2687 0.39 -0.3
3,176,416 + Pf6N2E2_2687 0.39 +1.9
3,176,416 + Pf6N2E2_2687 0.39 +0.0
3,176,417 - Pf6N2E2_2687 0.39 +0.4
3,176,417 - Pf6N2E2_2687 0.39 +0.5
3,176,428 + Pf6N2E2_2687 0.40 -0.4
3,176,429 - Pf6N2E2_2687 0.40 -1.1
3,176,438 - Pf6N2E2_2687 0.41 +0.8
3,176,606 - Pf6N2E2_2687 0.56 +0.7
3,176,714 - Pf6N2E2_2687 0.66 -1.1
3,176,714 - Pf6N2E2_2687 0.66 -1.7
3,176,821 + Pf6N2E2_2687 0.75 +1.9
3,176,822 - Pf6N2E2_2687 0.75 -0.0
3,176,872 + Pf6N2E2_2687 0.80 -1.1
3,176,872 + Pf6N2E2_2687 0.80 +0.3
3,176,872 + Pf6N2E2_2687 0.80 -1.9
3,176,873 - Pf6N2E2_2687 0.80 -0.2
3,177,174 + -1.4
3,177,174 + +0.9
3,177,212 - +1.9
3,177,229 - -2.3
3,177,229 - -1.2
3,177,707 + Pf6N2E2_2688 0.41 +0.3
3,177,794 + Pf6N2E2_2688 0.50 +1.9
3,177,815 - Pf6N2E2_2688 0.52 -2.2
3,177,821 - Pf6N2E2_2688 0.52 -0.4
3,177,821 - Pf6N2E2_2688 0.52 +0.3
3,178,037 + Pf6N2E2_2688 0.73 +1.9
3,178,192 + Pf6N2E2_2688 0.88 -2.5
3,178,253 - +0.4
3,178,316 + +0.2
3,178,316 + -0.1
3,178,415 - +0.1
3,178,417 - +0.3
3,178,417 - +1.9
3,178,570 - Pf6N2E2_2689 0.24 +0.8
3,178,629 - Pf6N2E2_2689 0.29 +1.2
3,178,871 + Pf6N2E2_2689 0.52 +0.7
3,178,872 - Pf6N2E2_2689 0.52 +1.1
3,178,872 - Pf6N2E2_2689 0.52 +0.3
3,178,878 - Pf6N2E2_2689 0.52 +1.9
3,179,026 - Pf6N2E2_2689 0.66 +0.4
3,179,118 + Pf6N2E2_2689 0.75 -0.1
3,179,137 + Pf6N2E2_2689 0.76 -0.3
3,179,277 + Pf6N2E2_2689 0.89 +0.9
3,179,278 - Pf6N2E2_2689 0.89 -0.8
3,179,309 + -0.4
3,179,310 - -1.3
3,179,310 - +0.9

Or see this region's nucleotide sequence