Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2321

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2319 and Pf6N2E2_2320 overlap by 8 nucleotidesPf6N2E2_2320 and Pf6N2E2_2321 are separated by 120 nucleotidesPf6N2E2_2321 and Pf6N2E2_2322 overlap by 8 nucleotidesPf6N2E2_2322 and Pf6N2E2_2323 overlap by 42 nucleotidesPf6N2E2_2323 and Pf6N2E2_2324 are separated by 76 nucleotides Pf6N2E2_2319 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 2,743,218 to 2,743,436 _2319 Pf6N2E2_2320 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 2,743,429 to 2,744,112 _2320 Pf6N2E2_2321 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 2,744,233 to 2,745,615 _2321 Pf6N2E2_2322 - hypothetical protein, at 2,745,608 to 2,745,751 _2322 Pf6N2E2_2323 - Fumarate and nitrate reduction regulatory protein, at 2,745,710 to 2,746,444 _2323 Pf6N2E2_2324 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 2,746,521 to 2,747,069 _2324 Position (kb) 2744 2745 2746Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4at 2743.334 kb on + strand, within Pf6N2E2_2319at 2743.582 kb on + strand, within Pf6N2E2_2320at 2743.582 kb on + strand, within Pf6N2E2_2320at 2743.606 kb on + strand, within Pf6N2E2_2320at 2743.607 kb on - strand, within Pf6N2E2_2320at 2743.607 kb on - strand, within Pf6N2E2_2320at 2743.717 kb on + strand, within Pf6N2E2_2320at 2743.718 kb on - strand, within Pf6N2E2_2320at 2744.194 kb on + strandat 2744.195 kb on - strandat 2744.296 kb on + strandat 2744.402 kb on - strand, within Pf6N2E2_2321at 2744.412 kb on - strand, within Pf6N2E2_2321at 2744.412 kb on - strand, within Pf6N2E2_2321at 2744.434 kb on + strand, within Pf6N2E2_2321at 2744.435 kb on - strand, within Pf6N2E2_2321at 2744.438 kb on - strand, within Pf6N2E2_2321at 2744.438 kb on - strand, within Pf6N2E2_2321at 2744.438 kb on - strand, within Pf6N2E2_2321at 2744.488 kb on + strand, within Pf6N2E2_2321at 2744.669 kb on - strand, within Pf6N2E2_2321at 2744.866 kb on - strand, within Pf6N2E2_2321at 2744.968 kb on + strand, within Pf6N2E2_2321at 2744.969 kb on - strand, within Pf6N2E2_2321at 2744.969 kb on - strand, within Pf6N2E2_2321at 2745.094 kb on + strand, within Pf6N2E2_2321at 2745.095 kb on - strand, within Pf6N2E2_2321at 2745.095 kb on - strand, within Pf6N2E2_2321at 2745.156 kb on + strand, within Pf6N2E2_2321at 2745.156 kb on + strand, within Pf6N2E2_2321at 2745.280 kb on + strand, within Pf6N2E2_2321at 2745.439 kb on + strand, within Pf6N2E2_2321at 2745.439 kb on + strand, within Pf6N2E2_2321at 2745.571 kb on + strandat 2745.572 kb on - strandat 2745.572 kb on - strandat 2745.610 kb on - strandat 2745.705 kb on - strand, within Pf6N2E2_2322at 2746.095 kb on + strand, within Pf6N2E2_2323at 2746.301 kb on + strand, within Pf6N2E2_2323at 2746.301 kb on + strand, within Pf6N2E2_2323at 2746.302 kb on - strand, within Pf6N2E2_2323at 2746.302 kb on - strand, within Pf6N2E2_2323at 2746.482 kb on - strandat 2746.484 kb on - strandat 2746.484 kb on - strandat 2746.605 kb on - strand, within Pf6N2E2_2324

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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2,743,334 + Pf6N2E2_2319 0.53 -6.8
2,743,582 + Pf6N2E2_2320 0.22 -6.7
2,743,582 + Pf6N2E2_2320 0.22 -4.5
2,743,606 + Pf6N2E2_2320 0.26 -4.8
2,743,607 - Pf6N2E2_2320 0.26 -4.4
2,743,607 - Pf6N2E2_2320 0.26 -4.6
2,743,717 + Pf6N2E2_2320 0.42 -7.0
2,743,718 - Pf6N2E2_2320 0.42 -4.5
2,744,194 + -1.9
2,744,195 - -1.7
2,744,296 + -4.5
2,744,402 - Pf6N2E2_2321 0.12 -1.6
2,744,412 - Pf6N2E2_2321 0.13 -2.7
2,744,412 - Pf6N2E2_2321 0.13 -3.0
2,744,434 + Pf6N2E2_2321 0.15 -0.6
2,744,435 - Pf6N2E2_2321 0.15 +2.0
2,744,438 - Pf6N2E2_2321 0.15 -3.8
2,744,438 - Pf6N2E2_2321 0.15 -2.4
2,744,438 - Pf6N2E2_2321 0.15 +0.0
2,744,488 + Pf6N2E2_2321 0.18 -3.5
2,744,669 - Pf6N2E2_2321 0.32 -2.3
2,744,866 - Pf6N2E2_2321 0.46 -0.2
2,744,968 + Pf6N2E2_2321 0.53 -2.6
2,744,969 - Pf6N2E2_2321 0.53 +0.0
2,744,969 - Pf6N2E2_2321 0.53 -3.5
2,745,094 + Pf6N2E2_2321 0.62 -0.9
2,745,095 - Pf6N2E2_2321 0.62 -2.0
2,745,095 - Pf6N2E2_2321 0.62 -4.2
2,745,156 + Pf6N2E2_2321 0.67 -2.4
2,745,156 + Pf6N2E2_2321 0.67 -2.9
2,745,280 + Pf6N2E2_2321 0.76 -3.2
2,745,439 + Pf6N2E2_2321 0.87 -1.0
2,745,439 + Pf6N2E2_2321 0.87 -0.6
2,745,571 + +1.3
2,745,572 - +0.3
2,745,572 - +0.4
2,745,610 - -0.3
2,745,705 - Pf6N2E2_2322 0.67 +1.3
2,746,095 + Pf6N2E2_2323 0.52 +2.0
2,746,301 + Pf6N2E2_2323 0.80 +0.0
2,746,301 + Pf6N2E2_2323 0.80 -0.3
2,746,302 - Pf6N2E2_2323 0.81 +4.0
2,746,302 - Pf6N2E2_2323 0.81 +1.0
2,746,482 - -0.6
2,746,484 - -0.4
2,746,484 - -1.2
2,746,605 - Pf6N2E2_2324 0.15 +0.2

Or see this region's nucleotide sequence