Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2319 and Pf6N2E2_2320 overlap by 8 nucleotides Pf6N2E2_2320 and Pf6N2E2_2321 are separated by 120 nucleotides Pf6N2E2_2321 and Pf6N2E2_2322 overlap by 8 nucleotides Pf6N2E2_2322 and Pf6N2E2_2323 overlap by 42 nucleotides Pf6N2E2_2323 and Pf6N2E2_2324 are separated by 76 nucleotides
Pf6N2E2_2319 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 2,743,218 to 2,743,436
_2319
Pf6N2E2_2320 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 2,743,429 to 2,744,112
_2320
Pf6N2E2_2321 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 2,744,233 to 2,745,615
_2321
Pf6N2E2_2322 - hypothetical protein, at 2,745,608 to 2,745,751
_2322
Pf6N2E2_2323 - Fumarate and nitrate reduction regulatory protein, at 2,745,710 to 2,746,444
_2323
Pf6N2E2_2324 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 2,746,521 to 2,747,069
_2324
Position (kb)
2744
2745
2746 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 2743.334 kb on + strand, within Pf6N2E2_2319 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.606 kb on + strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.717 kb on + strand, within Pf6N2E2_2320 at 2743.718 kb on - strand, within Pf6N2E2_2320 at 2744.194 kb on + strand at 2744.195 kb on - strand at 2744.296 kb on + strand at 2744.402 kb on - strand, within Pf6N2E2_2321 at 2744.412 kb on - strand, within Pf6N2E2_2321 at 2744.412 kb on - strand, within Pf6N2E2_2321 at 2744.434 kb on + strand, within Pf6N2E2_2321 at 2744.435 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.488 kb on + strand, within Pf6N2E2_2321 at 2744.669 kb on - strand, within Pf6N2E2_2321 at 2744.866 kb on - strand, within Pf6N2E2_2321 at 2744.968 kb on + strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2745.094 kb on + strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321 at 2745.156 kb on + strand, within Pf6N2E2_2321 at 2745.156 kb on + strand, within Pf6N2E2_2321 at 2745.280 kb on + strand, within Pf6N2E2_2321 at 2745.439 kb on + strand, within Pf6N2E2_2321 at 2745.439 kb on + strand, within Pf6N2E2_2321 at 2745.571 kb on + strand at 2745.572 kb on - strand at 2745.572 kb on - strand at 2745.610 kb on - strand at 2745.705 kb on - strand, within Pf6N2E2_2322 at 2746.095 kb on + strand, within Pf6N2E2_2323 at 2746.301 kb on + strand, within Pf6N2E2_2323 at 2746.301 kb on + strand, within Pf6N2E2_2323 at 2746.302 kb on - strand, within Pf6N2E2_2323 at 2746.302 kb on - strand, within Pf6N2E2_2323 at 2746.482 kb on - strand at 2746.484 kb on - strand at 2746.484 kb on - strand at 2746.605 kb on - strand, within Pf6N2E2_2324
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 2,743,334 + Pf6N2E2_2319 0.53 -6.8 2,743,582 + Pf6N2E2_2320 0.22 -6.7 2,743,582 + Pf6N2E2_2320 0.22 -4.5 2,743,606 + Pf6N2E2_2320 0.26 -4.8 2,743,607 - Pf6N2E2_2320 0.26 -4.4 2,743,607 - Pf6N2E2_2320 0.26 -4.6 2,743,717 + Pf6N2E2_2320 0.42 -7.0 2,743,718 - Pf6N2E2_2320 0.42 -4.5 2,744,194 + -1.9 2,744,195 - -1.7 2,744,296 + -4.5 2,744,402 - Pf6N2E2_2321 0.12 -1.6 2,744,412 - Pf6N2E2_2321 0.13 -2.7 2,744,412 - Pf6N2E2_2321 0.13 -3.0 2,744,434 + Pf6N2E2_2321 0.15 -0.6 2,744,435 - Pf6N2E2_2321 0.15 +2.0 2,744,438 - Pf6N2E2_2321 0.15 -3.8 2,744,438 - Pf6N2E2_2321 0.15 -2.4 2,744,438 - Pf6N2E2_2321 0.15 +0.0 2,744,488 + Pf6N2E2_2321 0.18 -3.5 2,744,669 - Pf6N2E2_2321 0.32 -2.3 2,744,866 - Pf6N2E2_2321 0.46 -0.2 2,744,968 + Pf6N2E2_2321 0.53 -2.6 2,744,969 - Pf6N2E2_2321 0.53 +0.0 2,744,969 - Pf6N2E2_2321 0.53 -3.5 2,745,094 + Pf6N2E2_2321 0.62 -0.9 2,745,095 - Pf6N2E2_2321 0.62 -2.0 2,745,095 - Pf6N2E2_2321 0.62 -4.2 2,745,156 + Pf6N2E2_2321 0.67 -2.4 2,745,156 + Pf6N2E2_2321 0.67 -2.9 2,745,280 + Pf6N2E2_2321 0.76 -3.2 2,745,439 + Pf6N2E2_2321 0.87 -1.0 2,745,439 + Pf6N2E2_2321 0.87 -0.6 2,745,571 + +1.3 2,745,572 - +0.3 2,745,572 - +0.4 2,745,610 - -0.3 2,745,705 - Pf6N2E2_2322 0.67 +1.3 2,746,095 + Pf6N2E2_2323 0.52 +2.0 2,746,301 + Pf6N2E2_2323 0.80 +0.0 2,746,301 + Pf6N2E2_2323 0.80 -0.3 2,746,302 - Pf6N2E2_2323 0.81 +4.0 2,746,302 - Pf6N2E2_2323 0.81 +1.0 2,746,482 - -0.6 2,746,484 - -0.4 2,746,484 - -1.2 2,746,605 - Pf6N2E2_2324 0.15 +0.2
Or see this region's nucleotide sequence