Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2134 and Pf6N2E2_2135 are separated by 343 nucleotides Pf6N2E2_2135 and Pf6N2E2_2136 are separated by 101 nucleotides
Pf6N2E2_2134 - putative lipoprotein, at 2,539,320 to 2,540,069
_2134
Pf6N2E2_2135 - Aldose 1-epimerase (EC 5.1.3.3), at 2,540,413 to 2,541,519
_2135
Pf6N2E2_2136 - Oxidoreductase, aldo/keto reductase family, at 2,541,621 to 2,542,442
_2136
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2539.600 kb on + strand, within Pf6N2E2_2134 at 2539.600 kb on + strand, within Pf6N2E2_2134 at 2539.600 kb on + strand, within Pf6N2E2_2134 at 2539.600 kb on + strand, within Pf6N2E2_2134 at 2539.601 kb on - strand, within Pf6N2E2_2134 at 2539.601 kb on - strand, within Pf6N2E2_2134 at 2539.601 kb on - strand, within Pf6N2E2_2134 at 2539.601 kb on - strand, within Pf6N2E2_2134 at 2539.651 kb on + strand, within Pf6N2E2_2134 at 2539.651 kb on + strand, within Pf6N2E2_2134 at 2539.651 kb on + strand, within Pf6N2E2_2134 at 2539.652 kb on - strand, within Pf6N2E2_2134 at 2539.652 kb on - strand, within Pf6N2E2_2134 at 2539.652 kb on - strand, within Pf6N2E2_2134 at 2539.652 kb on - strand, within Pf6N2E2_2134 at 2539.652 kb on - strand, within Pf6N2E2_2134 at 2539.699 kb on + strand, within Pf6N2E2_2134 at 2539.700 kb on - strand, within Pf6N2E2_2134 at 2539.700 kb on - strand, within Pf6N2E2_2134 at 2539.700 kb on - strand, within Pf6N2E2_2134 at 2539.700 kb on - strand, within Pf6N2E2_2134 at 2539.838 kb on - strand, within Pf6N2E2_2134 at 2539.838 kb on - strand, within Pf6N2E2_2134 at 2539.858 kb on + strand, within Pf6N2E2_2134 at 2539.858 kb on + strand, within Pf6N2E2_2134 at 2539.859 kb on - strand, within Pf6N2E2_2134 at 2540.274 kb on + strand at 2540.364 kb on + strand at 2540.364 kb on + strand at 2540.364 kb on + strand at 2540.364 kb on + strand at 2540.365 kb on - strand at 2540.365 kb on - strand at 2540.391 kb on + strand at 2540.452 kb on - strand at 2540.548 kb on + strand, within Pf6N2E2_2135 at 2540.549 kb on - strand, within Pf6N2E2_2135 at 2540.558 kb on + strand, within Pf6N2E2_2135 at 2540.558 kb on + strand, within Pf6N2E2_2135 at 2540.558 kb on + strand, within Pf6N2E2_2135 at 2540.559 kb on - strand, within Pf6N2E2_2135 at 2540.787 kb on + strand, within Pf6N2E2_2135 at 2540.905 kb on + strand, within Pf6N2E2_2135 at 2540.905 kb on + strand, within Pf6N2E2_2135 at 2540.906 kb on - strand, within Pf6N2E2_2135 at 2540.906 kb on - strand, within Pf6N2E2_2135 at 2540.942 kb on - strand, within Pf6N2E2_2135 at 2540.942 kb on - strand, within Pf6N2E2_2135 at 2540.992 kb on + strand, within Pf6N2E2_2135 at 2540.993 kb on - strand, within Pf6N2E2_2135 at 2541.061 kb on + strand, within Pf6N2E2_2135 at 2541.061 kb on + strand, within Pf6N2E2_2135 at 2541.062 kb on - strand, within Pf6N2E2_2135 at 2541.062 kb on - strand, within Pf6N2E2_2135 at 2541.117 kb on + strand, within Pf6N2E2_2135 at 2541.159 kb on + strand, within Pf6N2E2_2135 at 2541.271 kb on + strand, within Pf6N2E2_2135 at 2541.272 kb on - strand, within Pf6N2E2_2135 at 2541.272 kb on - strand, within Pf6N2E2_2135 at 2541.272 kb on - strand, within Pf6N2E2_2135 at 2541.272 kb on - strand, within Pf6N2E2_2135 at 2541.304 kb on + strand, within Pf6N2E2_2135 at 2541.304 kb on + strand, within Pf6N2E2_2135 at 2541.304 kb on + strand, within Pf6N2E2_2135 at 2541.305 kb on - strand, within Pf6N2E2_2135 at 2541.305 kb on - strand, within Pf6N2E2_2135 at 2541.305 kb on - strand, within Pf6N2E2_2135 at 2541.332 kb on - strand, within Pf6N2E2_2135 at 2541.332 kb on - strand, within Pf6N2E2_2135 at 2541.332 kb on - strand, within Pf6N2E2_2135 at 2541.382 kb on + strand, within Pf6N2E2_2135 at 2541.481 kb on + strand at 2541.481 kb on + strand at 2541.481 kb on + strand at 2541.482 kb on - strand at 2541.482 kb on - strand at 2541.518 kb on - strand at 2541.518 kb on - strand at 2541.579 kb on + strand at 2541.579 kb on + strand at 2541.616 kb on + strand at 2541.616 kb on + strand at 2541.616 kb on + strand at 2541.617 kb on - strand at 2541.617 kb on - strand at 2541.617 kb on - strand at 2541.939 kb on + strand, within Pf6N2E2_2136 at 2541.939 kb on + strand, within Pf6N2E2_2136 at 2541.939 kb on + strand, within Pf6N2E2_2136 at 2541.939 kb on + strand, within Pf6N2E2_2136 at 2541.940 kb on - strand, within Pf6N2E2_2136 at 2541.940 kb on - strand, within Pf6N2E2_2136 at 2541.940 kb on - strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.101 kb on + strand, within Pf6N2E2_2136 at 2542.102 kb on - strand, within Pf6N2E2_2136 at 2542.102 kb on - strand, within Pf6N2E2_2136 at 2542.102 kb on - strand, within Pf6N2E2_2136 at 2542.102 kb on - strand, within Pf6N2E2_2136 at 2542.176 kb on + strand, within Pf6N2E2_2136 at 2542.399 kb on + strand at 2542.400 kb on - strand at 2542.440 kb on + strand at 2542.441 kb on - strand at 2542.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 2,539,600 + Pf6N2E2_2134 0.37 -3.0 2,539,600 + Pf6N2E2_2134 0.37 -0.4 2,539,600 + Pf6N2E2_2134 0.37 -1.1 2,539,600 + Pf6N2E2_2134 0.37 -0.5 2,539,601 - Pf6N2E2_2134 0.37 -0.0 2,539,601 - Pf6N2E2_2134 0.37 -0.4 2,539,601 - Pf6N2E2_2134 0.37 +0.2 2,539,601 - Pf6N2E2_2134 0.37 -0.1 2,539,651 + Pf6N2E2_2134 0.44 +0.4 2,539,651 + Pf6N2E2_2134 0.44 -1.4 2,539,651 + Pf6N2E2_2134 0.44 +2.0 2,539,652 - Pf6N2E2_2134 0.44 +0.3 2,539,652 - Pf6N2E2_2134 0.44 +0.4 2,539,652 - Pf6N2E2_2134 0.44 +0.5 2,539,652 - Pf6N2E2_2134 0.44 -0.4 2,539,652 - Pf6N2E2_2134 0.44 -0.3 2,539,699 + Pf6N2E2_2134 0.51 -0.2 2,539,700 - Pf6N2E2_2134 0.51 -2.5 2,539,700 - Pf6N2E2_2134 0.51 -0.4 2,539,700 - Pf6N2E2_2134 0.51 +2.0 2,539,700 - Pf6N2E2_2134 0.51 +0.1 2,539,838 - Pf6N2E2_2134 0.69 -0.9 2,539,838 - Pf6N2E2_2134 0.69 -0.9 2,539,858 + Pf6N2E2_2134 0.72 -0.3 2,539,858 + Pf6N2E2_2134 0.72 +1.0 2,539,859 - Pf6N2E2_2134 0.72 +1.6 2,540,274 + +1.0 2,540,364 + -0.9 2,540,364 + -2.2 2,540,364 + +0.6 2,540,364 + -1.0 2,540,365 - +0.4 2,540,365 - -0.1 2,540,391 + -0.4 2,540,452 - -0.0 2,540,548 + Pf6N2E2_2135 0.12 -0.9 2,540,549 - Pf6N2E2_2135 0.12 +0.8 2,540,558 + Pf6N2E2_2135 0.13 +1.0 2,540,558 + Pf6N2E2_2135 0.13 -0.4 2,540,558 + Pf6N2E2_2135 0.13 +0.2 2,540,559 - Pf6N2E2_2135 0.13 -1.2 2,540,787 + Pf6N2E2_2135 0.34 +0.4 2,540,905 + Pf6N2E2_2135 0.44 -0.4 2,540,905 + Pf6N2E2_2135 0.44 -1.4 2,540,906 - Pf6N2E2_2135 0.45 +0.0 2,540,906 - Pf6N2E2_2135 0.45 -1.6 2,540,942 - Pf6N2E2_2135 0.48 +0.4 2,540,942 - Pf6N2E2_2135 0.48 +0.2 2,540,992 + Pf6N2E2_2135 0.52 +0.4 2,540,993 - Pf6N2E2_2135 0.52 -0.2 2,541,061 + Pf6N2E2_2135 0.59 -2.4 2,541,061 + Pf6N2E2_2135 0.59 +2.0 2,541,062 - Pf6N2E2_2135 0.59 -0.4 2,541,062 - Pf6N2E2_2135 0.59 -2.3 2,541,117 + Pf6N2E2_2135 0.64 +0.4 2,541,159 + Pf6N2E2_2135 0.67 -1.4 2,541,271 + Pf6N2E2_2135 0.78 +0.0 2,541,272 - Pf6N2E2_2135 0.78 +0.6 2,541,272 - Pf6N2E2_2135 0.78 +0.6 2,541,272 - Pf6N2E2_2135 0.78 -1.1 2,541,272 - Pf6N2E2_2135 0.78 +0.3 2,541,304 + Pf6N2E2_2135 0.80 -0.4 2,541,304 + Pf6N2E2_2135 0.80 -0.6 2,541,304 + Pf6N2E2_2135 0.80 +1.3 2,541,305 - Pf6N2E2_2135 0.81 +0.4 2,541,305 - Pf6N2E2_2135 0.81 -0.4 2,541,305 - Pf6N2E2_2135 0.81 -0.6 2,541,332 - Pf6N2E2_2135 0.83 -2.8 2,541,332 - Pf6N2E2_2135 0.83 -2.1 2,541,332 - Pf6N2E2_2135 0.83 -1.8 2,541,382 + Pf6N2E2_2135 0.88 -1.5 2,541,481 + -0.7 2,541,481 + -0.4 2,541,481 + +0.9 2,541,482 - +0.5 2,541,482 - +1.2 2,541,518 - +0.6 2,541,518 - -1.1 2,541,579 + -0.5 2,541,579 + +0.6 2,541,616 + -0.9 2,541,616 + +0.3 2,541,616 + +0.4 2,541,617 - -0.3 2,541,617 - +0.3 2,541,617 - +0.0 2,541,939 + Pf6N2E2_2136 0.39 +2.8 2,541,939 + Pf6N2E2_2136 0.39 -0.2 2,541,939 + Pf6N2E2_2136 0.39 -1.2 2,541,939 + Pf6N2E2_2136 0.39 -3.4 2,541,940 - Pf6N2E2_2136 0.39 -3.6 2,541,940 - Pf6N2E2_2136 0.39 +0.4 2,541,940 - Pf6N2E2_2136 0.39 -2.1 2,542,101 + Pf6N2E2_2136 0.58 -0.3 2,542,101 + Pf6N2E2_2136 0.58 -0.4 2,542,101 + Pf6N2E2_2136 0.58 +0.6 2,542,101 + Pf6N2E2_2136 0.58 +2.0 2,542,101 + Pf6N2E2_2136 0.58 -1.4 2,542,101 + Pf6N2E2_2136 0.58 +2.0 2,542,101 + Pf6N2E2_2136 0.58 +0.7 2,542,101 + Pf6N2E2_2136 0.58 +0.9 2,542,102 - Pf6N2E2_2136 0.59 -0.8 2,542,102 - Pf6N2E2_2136 0.59 -0.0 2,542,102 - Pf6N2E2_2136 0.59 -1.4 2,542,102 - Pf6N2E2_2136 0.59 +1.2 2,542,176 + Pf6N2E2_2136 0.68 -0.6 2,542,399 + +0.6 2,542,400 - -1.5 2,542,440 + +2.0 2,542,441 - +0.8 2,542,441 - +0.3
Or see this region's nucleotide sequence