Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_203 and Pf6N2E2_204 are separated by 2 nucleotides Pf6N2E2_204 and Pf6N2E2_205 overlap by 4 nucleotides Pf6N2E2_205 and Pf6N2E2_206 are separated by 32 nucleotides
Pf6N2E2_203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 254,640 to 255,920
_203
Pf6N2E2_204 - hypothetical protein, at 255,923 to 256,330
_204
Pf6N2E2_205 - hypothetical protein, at 256,327 to 257,073
_205
Pf6N2E2_206 - Glycosyl transferase, group 2 family protein, at 257,106 to 258,128
_206
Position (kb)
255
256
257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 254.960 kb on + strand, within Pf6N2E2_203 at 255.029 kb on - strand, within Pf6N2E2_203 at 255.232 kb on - strand, within Pf6N2E2_203 at 255.384 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.417 kb on - strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.806 kb on - strand at 255.806 kb on - strand at 255.806 kb on - strand at 255.890 kb on - strand at 255.912 kb on + strand at 256.062 kb on + strand, within Pf6N2E2_204 at 256.062 kb on + strand, within Pf6N2E2_204 at 256.062 kb on + strand, within Pf6N2E2_204 at 256.321 kb on + strand at 256.478 kb on + strand, within Pf6N2E2_205 at 256.478 kb on + strand, within Pf6N2E2_205 at 256.479 kb on - strand, within Pf6N2E2_205 at 256.562 kb on + strand, within Pf6N2E2_205 at 256.562 kb on + strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.659 kb on + strand, within Pf6N2E2_205 at 256.661 kb on + strand, within Pf6N2E2_205 at 256.661 kb on + strand, within Pf6N2E2_205 at 256.882 kb on + strand, within Pf6N2E2_205 at 256.883 kb on - strand, within Pf6N2E2_205 at 256.892 kb on + strand, within Pf6N2E2_205 at 256.956 kb on - strand, within Pf6N2E2_205 at 257.148 kb on - strand at 257.305 kb on + strand, within Pf6N2E2_206 at 257.305 kb on + strand, within Pf6N2E2_206 at 257.306 kb on - strand, within Pf6N2E2_206 at 257.306 kb on - strand, within Pf6N2E2_206 at 257.306 kb on - strand, within Pf6N2E2_206
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 254,960 + Pf6N2E2_203 0.25 -2.3 255,029 - Pf6N2E2_203 0.30 -0.3 255,232 - Pf6N2E2_203 0.46 -0.1 255,384 + Pf6N2E2_203 0.58 +0.8 255,416 + Pf6N2E2_203 0.61 -0.7 255,416 + Pf6N2E2_203 0.61 -0.8 255,416 + Pf6N2E2_203 0.61 +0.6 255,416 + Pf6N2E2_203 0.61 -0.3 255,416 + Pf6N2E2_203 0.61 -1.5 255,416 + Pf6N2E2_203 0.61 -0.9 255,417 - Pf6N2E2_203 0.61 -3.1 255,514 + Pf6N2E2_203 0.68 -0.9 255,514 + Pf6N2E2_203 0.68 -0.2 255,514 + Pf6N2E2_203 0.68 -1.3 255,515 - Pf6N2E2_203 0.68 +1.9 255,515 - Pf6N2E2_203 0.68 -0.6 255,515 - Pf6N2E2_203 0.68 +0.8 255,803 + +0.9 255,803 + -0.1 255,803 + +0.5 255,803 + +0.4 255,803 + -0.4 255,803 + +0.0 255,803 + +0.1 255,805 + +1.9 255,805 + +0.3 255,805 + -0.5 255,805 + +0.9 255,806 - +0.3 255,806 - +0.0 255,806 - +0.6 255,890 - -0.5 255,912 + -3.4 256,062 + Pf6N2E2_204 0.34 +0.3 256,062 + Pf6N2E2_204 0.34 +0.9 256,062 + Pf6N2E2_204 0.34 -2.7 256,321 + +1.1 256,478 + Pf6N2E2_205 0.20 +1.5 256,478 + Pf6N2E2_205 0.20 +0.9 256,479 - Pf6N2E2_205 0.20 +0.6 256,562 + Pf6N2E2_205 0.31 +0.9 256,562 + Pf6N2E2_205 0.31 -0.5 256,563 - Pf6N2E2_205 0.32 -0.1 256,563 - Pf6N2E2_205 0.32 -0.8 256,563 - Pf6N2E2_205 0.32 +0.1 256,659 + Pf6N2E2_205 0.44 -0.5 256,661 + Pf6N2E2_205 0.45 +1.6 256,661 + Pf6N2E2_205 0.45 -2.4 256,882 + Pf6N2E2_205 0.74 -0.4 256,883 - Pf6N2E2_205 0.74 +1.9 256,892 + Pf6N2E2_205 0.76 +1.9 256,956 - Pf6N2E2_205 0.84 -2.4 257,148 - +0.9 257,305 + Pf6N2E2_206 0.19 +0.3 257,305 + Pf6N2E2_206 0.19 +0.1 257,306 - Pf6N2E2_206 0.20 -1.8 257,306 - Pf6N2E2_206 0.20 -1.3 257,306 - Pf6N2E2_206 0.20 -0.9
Or see this region's nucleotide sequence