Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_204

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_203 and Pf6N2E2_204 are separated by 2 nucleotidesPf6N2E2_204 and Pf6N2E2_205 overlap by 4 nucleotidesPf6N2E2_205 and Pf6N2E2_206 are separated by 32 nucleotides Pf6N2E2_203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 254,640 to 255,920 _203 Pf6N2E2_204 - hypothetical protein, at 255,923 to 256,330 _204 Pf6N2E2_205 - hypothetical protein, at 256,327 to 257,073 _205 Pf6N2E2_206 - Glycosyl transferase, group 2 family protein, at 257,106 to 258,128 _206 Position (kb) 255 256 257Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 254.960 kb on + strand, within Pf6N2E2_203at 255.029 kb on - strand, within Pf6N2E2_203at 255.232 kb on - strand, within Pf6N2E2_203at 255.384 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.416 kb on + strand, within Pf6N2E2_203at 255.417 kb on - strand, within Pf6N2E2_203at 255.514 kb on + strand, within Pf6N2E2_203at 255.514 kb on + strand, within Pf6N2E2_203at 255.514 kb on + strand, within Pf6N2E2_203at 255.515 kb on - strand, within Pf6N2E2_203at 255.515 kb on - strand, within Pf6N2E2_203at 255.515 kb on - strand, within Pf6N2E2_203at 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.803 kb on + strandat 255.805 kb on + strandat 255.805 kb on + strandat 255.805 kb on + strandat 255.805 kb on + strandat 255.806 kb on - strandat 255.806 kb on - strandat 255.806 kb on - strandat 255.890 kb on - strandat 255.912 kb on + strandat 256.062 kb on + strand, within Pf6N2E2_204at 256.062 kb on + strand, within Pf6N2E2_204at 256.062 kb on + strand, within Pf6N2E2_204at 256.321 kb on + strandat 256.478 kb on + strand, within Pf6N2E2_205at 256.478 kb on + strand, within Pf6N2E2_205at 256.479 kb on - strand, within Pf6N2E2_205at 256.562 kb on + strand, within Pf6N2E2_205at 256.562 kb on + strand, within Pf6N2E2_205at 256.563 kb on - strand, within Pf6N2E2_205at 256.563 kb on - strand, within Pf6N2E2_205at 256.563 kb on - strand, within Pf6N2E2_205at 256.659 kb on + strand, within Pf6N2E2_205at 256.661 kb on + strand, within Pf6N2E2_205at 256.661 kb on + strand, within Pf6N2E2_205at 256.882 kb on + strand, within Pf6N2E2_205at 256.883 kb on - strand, within Pf6N2E2_205at 256.892 kb on + strand, within Pf6N2E2_205at 256.956 kb on - strand, within Pf6N2E2_205at 257.148 kb on - strandat 257.305 kb on + strand, within Pf6N2E2_206at 257.305 kb on + strand, within Pf6N2E2_206at 257.306 kb on - strand, within Pf6N2E2_206at 257.306 kb on - strand, within Pf6N2E2_206at 257.306 kb on - strand, within Pf6N2E2_206

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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254,960 + Pf6N2E2_203 0.25 -2.3
255,029 - Pf6N2E2_203 0.30 -0.3
255,232 - Pf6N2E2_203 0.46 -0.1
255,384 + Pf6N2E2_203 0.58 +0.8
255,416 + Pf6N2E2_203 0.61 -0.7
255,416 + Pf6N2E2_203 0.61 -0.8
255,416 + Pf6N2E2_203 0.61 +0.6
255,416 + Pf6N2E2_203 0.61 -0.3
255,416 + Pf6N2E2_203 0.61 -1.5
255,416 + Pf6N2E2_203 0.61 -0.9
255,417 - Pf6N2E2_203 0.61 -3.1
255,514 + Pf6N2E2_203 0.68 -0.9
255,514 + Pf6N2E2_203 0.68 -0.2
255,514 + Pf6N2E2_203 0.68 -1.3
255,515 - Pf6N2E2_203 0.68 +1.9
255,515 - Pf6N2E2_203 0.68 -0.6
255,515 - Pf6N2E2_203 0.68 +0.8
255,803 + +0.9
255,803 + -0.1
255,803 + +0.5
255,803 + +0.4
255,803 + -0.4
255,803 + +0.0
255,803 + +0.1
255,805 + +1.9
255,805 + +0.3
255,805 + -0.5
255,805 + +0.9
255,806 - +0.3
255,806 - +0.0
255,806 - +0.6
255,890 - -0.5
255,912 + -3.4
256,062 + Pf6N2E2_204 0.34 +0.3
256,062 + Pf6N2E2_204 0.34 +0.9
256,062 + Pf6N2E2_204 0.34 -2.7
256,321 + +1.1
256,478 + Pf6N2E2_205 0.20 +1.5
256,478 + Pf6N2E2_205 0.20 +0.9
256,479 - Pf6N2E2_205 0.20 +0.6
256,562 + Pf6N2E2_205 0.31 +0.9
256,562 + Pf6N2E2_205 0.31 -0.5
256,563 - Pf6N2E2_205 0.32 -0.1
256,563 - Pf6N2E2_205 0.32 -0.8
256,563 - Pf6N2E2_205 0.32 +0.1
256,659 + Pf6N2E2_205 0.44 -0.5
256,661 + Pf6N2E2_205 0.45 +1.6
256,661 + Pf6N2E2_205 0.45 -2.4
256,882 + Pf6N2E2_205 0.74 -0.4
256,883 - Pf6N2E2_205 0.74 +1.9
256,892 + Pf6N2E2_205 0.76 +1.9
256,956 - Pf6N2E2_205 0.84 -2.4
257,148 - +0.9
257,305 + Pf6N2E2_206 0.19 +0.3
257,305 + Pf6N2E2_206 0.19 +0.1
257,306 - Pf6N2E2_206 0.20 -1.8
257,306 - Pf6N2E2_206 0.20 -1.3
257,306 - Pf6N2E2_206 0.20 -0.9

Or see this region's nucleotide sequence