Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2039

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2037 and Pf6N2E2_2038 are separated by 105 nucleotidesPf6N2E2_2038 and Pf6N2E2_2039 overlap by 1 nucleotidesPf6N2E2_2039 and Pf6N2E2_2040 are separated by 302 nucleotides Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704 _2037 Pf6N2E2_2038 - Transcriptional regulator, TetR family, at 2,426,810 to 2,427,469 _2038 Pf6N2E2_2039 - Sigma-54 dependent transcriptional regulator, at 2,427,469 to 2,428,884 _2039 Pf6N2E2_2040 - D-beta-hydroxybutyrate permease, at 2,429,187 to 2,430,578 _2040 Position (kb) 2427 2428 2429Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2426.677 kb on + strandat 2426.869 kb on + strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.872 kb on - strandat 2426.872 kb on - strandat 2427.197 kb on + strand, within Pf6N2E2_2038at 2427.197 kb on + strand, within Pf6N2E2_2038at 2427.215 kb on + strand, within Pf6N2E2_2038at 2427.215 kb on + strand, within Pf6N2E2_2038at 2427.216 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.496 kb on + strandat 2427.496 kb on + strandat 2427.610 kb on + strandat 2427.610 kb on + strandat 2427.862 kb on + strand, within Pf6N2E2_2039at 2427.925 kb on + strand, within Pf6N2E2_2039at 2427.926 kb on - strand, within Pf6N2E2_2039at 2428.228 kb on - strand, within Pf6N2E2_2039at 2428.306 kb on + strand, within Pf6N2E2_2039at 2428.417 kb on + strand, within Pf6N2E2_2039at 2428.553 kb on + strand, within Pf6N2E2_2039at 2428.554 kb on - strand, within Pf6N2E2_2039at 2428.554 kb on - strand, within Pf6N2E2_2039at 2428.677 kb on - strand, within Pf6N2E2_2039at 2428.843 kb on + strandat 2428.844 kb on - strandat 2428.844 kb on - strandat 2428.882 kb on + strandat 2428.904 kb on + strandat 2428.906 kb on + strandat 2428.983 kb on - strandat 2429.167 kb on - strandat 2429.371 kb on - strand, within Pf6N2E2_2040at 2429.468 kb on + strand, within Pf6N2E2_2040at 2429.517 kb on + strand, within Pf6N2E2_2040at 2429.517 kb on + strand, within Pf6N2E2_2040at 2429.517 kb on + strand, within Pf6N2E2_2040at 2429.518 kb on - strand, within Pf6N2E2_2040at 2429.518 kb on - strand, within Pf6N2E2_2040at 2429.518 kb on - strand, within Pf6N2E2_2040at 2429.588 kb on + strand, within Pf6N2E2_2040at 2429.588 kb on + strand, within Pf6N2E2_2040at 2429.589 kb on - strand, within Pf6N2E2_2040at 2429.589 kb on - strand, within Pf6N2E2_2040at 2429.591 kb on + strand, within Pf6N2E2_2040at 2429.591 kb on + strand, within Pf6N2E2_2040at 2429.591 kb on + strand, within Pf6N2E2_2040at 2429.591 kb on + strand, within Pf6N2E2_2040at 2429.592 kb on - strand, within Pf6N2E2_2040at 2429.592 kb on - strand, within Pf6N2E2_2040

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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2,426,677 + -0.4
2,426,869 + +1.9
2,426,870 - +0.3
2,426,870 - -3.0
2,426,870 - +0.2
2,426,870 - +1.1
2,426,870 - +0.6
2,426,872 - -1.7
2,426,872 - -1.4
2,427,197 + Pf6N2E2_2038 0.59 +0.3
2,427,197 + Pf6N2E2_2038 0.59 +0.9
2,427,215 + Pf6N2E2_2038 0.61 -2.0
2,427,215 + Pf6N2E2_2038 0.61 +1.9
2,427,216 - Pf6N2E2_2038 0.62 -1.4
2,427,393 - Pf6N2E2_2038 0.88 +0.2
2,427,393 - Pf6N2E2_2038 0.88 -2.6
2,427,393 - Pf6N2E2_2038 0.88 +0.9
2,427,393 - Pf6N2E2_2038 0.88 +0.9
2,427,496 + -2.7
2,427,496 + -0.2
2,427,610 + +2.1
2,427,610 + -0.7
2,427,862 + Pf6N2E2_2039 0.28 +0.9
2,427,925 + Pf6N2E2_2039 0.32 +0.1
2,427,926 - Pf6N2E2_2039 0.32 +0.3
2,428,228 - Pf6N2E2_2039 0.54 +2.5
2,428,306 + Pf6N2E2_2039 0.59 +1.3
2,428,417 + Pf6N2E2_2039 0.67 +1.6
2,428,553 + Pf6N2E2_2039 0.77 -0.3
2,428,554 - Pf6N2E2_2039 0.77 -0.2
2,428,554 - Pf6N2E2_2039 0.77 +0.0
2,428,677 - Pf6N2E2_2039 0.85 +0.8
2,428,843 + -1.7
2,428,844 - -0.3
2,428,844 - -1.3
2,428,882 + +1.9
2,428,904 + -2.8
2,428,906 + +1.9
2,428,983 - +1.9
2,429,167 - -1.3
2,429,371 - Pf6N2E2_2040 0.13 +0.9
2,429,468 + Pf6N2E2_2040 0.20 -0.1
2,429,517 + Pf6N2E2_2040 0.24 -0.4
2,429,517 + Pf6N2E2_2040 0.24 -1.3
2,429,517 + Pf6N2E2_2040 0.24 -2.0
2,429,518 - Pf6N2E2_2040 0.24 -1.1
2,429,518 - Pf6N2E2_2040 0.24 +0.6
2,429,518 - Pf6N2E2_2040 0.24 +1.9
2,429,588 + Pf6N2E2_2040 0.29 -0.8
2,429,588 + Pf6N2E2_2040 0.29 -1.1
2,429,589 - Pf6N2E2_2040 0.29 -0.1
2,429,589 - Pf6N2E2_2040 0.29 +1.1
2,429,591 + Pf6N2E2_2040 0.29 +1.9
2,429,591 + Pf6N2E2_2040 0.29 +0.4
2,429,591 + Pf6N2E2_2040 0.29 -1.3
2,429,591 + Pf6N2E2_2040 0.29 -3.7
2,429,592 - Pf6N2E2_2040 0.29 -2.7
2,429,592 - Pf6N2E2_2040 0.29 +0.8

Or see this region's nucleotide sequence