Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2035 and Pf6N2E2_2036 are separated by 10 nucleotides Pf6N2E2_2036 and Pf6N2E2_2037 are separated by 56 nucleotides
Pf6N2E2_2035 - epoxide hydrolase, at 2,424,362 to 2,425,366
_2035
Pf6N2E2_2036 - Antibiotic biosynthesis monooxygenase, at 2,425,377 to 2,425,754
_2036
Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704
_2037
Position (kb)
2425
2426 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2424.600 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.604 kb on - strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.771 kb on + strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035 at 2425.345 kb on - strand at 2425.345 kb on - strand at 2425.367 kb on - strand at 2425.367 kb on - strand at 2425.447 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.668 kb on - strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.672 kb on - strand, within Pf6N2E2_2036 at 2425.718 kb on + strand at 2425.726 kb on - strand at 2425.726 kb on - strand at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2426.229 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.348 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.677 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 2,424,600 + Pf6N2E2_2035 0.24 +0.9 2,424,603 + Pf6N2E2_2035 0.24 +0.5 2,424,603 + Pf6N2E2_2035 0.24 -0.1 2,424,603 + Pf6N2E2_2035 0.24 -1.4 2,424,604 - Pf6N2E2_2035 0.24 +0.3 2,424,644 + Pf6N2E2_2035 0.28 +0.9 2,424,644 + Pf6N2E2_2035 0.28 +0.7 2,424,644 + Pf6N2E2_2035 0.28 -0.7 2,424,645 - Pf6N2E2_2035 0.28 +1.9 2,424,645 - Pf6N2E2_2035 0.28 +0.4 2,424,645 - Pf6N2E2_2035 0.28 -1.0 2,424,645 - Pf6N2E2_2035 0.28 -0.4 2,424,771 + Pf6N2E2_2035 0.41 -2.9 2,424,772 - Pf6N2E2_2035 0.41 -0.0 2,424,772 - Pf6N2E2_2035 0.41 -0.4 2,424,772 - Pf6N2E2_2035 0.41 +0.5 2,424,772 - Pf6N2E2_2035 0.41 +1.9 2,424,772 - Pf6N2E2_2035 0.41 -0.6 2,424,772 - Pf6N2E2_2035 0.41 -0.1 2,424,888 + Pf6N2E2_2035 0.52 +0.4 2,424,888 + Pf6N2E2_2035 0.52 -0.9 2,424,888 + Pf6N2E2_2035 0.52 -0.2 2,424,888 + Pf6N2E2_2035 0.52 +0.6 2,424,889 - Pf6N2E2_2035 0.52 +0.2 2,424,889 - Pf6N2E2_2035 0.52 +0.9 2,425,345 - +0.9 2,425,345 - +0.1 2,425,367 - +0.1 2,425,367 - -0.1 2,425,447 + Pf6N2E2_2036 0.19 +0.5 2,425,522 + Pf6N2E2_2036 0.38 -0.1 2,425,522 + +0.7 2,425,522 + Pf6N2E2_2036 0.38 +0.9 2,425,522 + Pf6N2E2_2036 0.38 -3.0 2,425,522 + Pf6N2E2_2036 0.38 +0.3 2,425,522 + Pf6N2E2_2036 0.38 +1.0 2,425,522 + Pf6N2E2_2036 0.38 +0.5 2,425,522 + Pf6N2E2_2036 0.38 -0.4 2,425,523 - Pf6N2E2_2036 0.39 +0.3 2,425,523 - Pf6N2E2_2036 0.39 -1.3 2,425,523 - Pf6N2E2_2036 0.39 -0.2 2,425,523 - Pf6N2E2_2036 0.39 +1.9 2,425,523 - Pf6N2E2_2036 0.39 +1.9 2,425,659 + Pf6N2E2_2036 0.75 -2.5 2,425,659 + Pf6N2E2_2036 0.75 -0.2 2,425,659 + Pf6N2E2_2036 0.75 -2.1 2,425,659 + Pf6N2E2_2036 0.75 +1.4 2,425,659 + Pf6N2E2_2036 0.75 +0.7 2,425,659 + Pf6N2E2_2036 0.75 -0.5 2,425,660 - Pf6N2E2_2036 0.75 +1.9 2,425,660 - Pf6N2E2_2036 0.75 -2.4 2,425,660 - Pf6N2E2_2036 0.75 -0.1 2,425,660 - Pf6N2E2_2036 0.75 +0.4 2,425,660 - Pf6N2E2_2036 0.75 +1.3 2,425,667 + Pf6N2E2_2036 0.77 -1.2 2,425,667 + Pf6N2E2_2036 0.77 +0.9 2,425,667 + Pf6N2E2_2036 0.77 +1.5 2,425,667 + Pf6N2E2_2036 0.77 -0.1 2,425,667 + Pf6N2E2_2036 0.77 -0.1 2,425,667 + Pf6N2E2_2036 0.77 +0.6 2,425,668 - Pf6N2E2_2036 0.77 -0.2 2,425,671 + Pf6N2E2_2036 0.78 +1.9 2,425,671 + Pf6N2E2_2036 0.78 -3.8 2,425,671 + Pf6N2E2_2036 0.78 +0.1 2,425,672 - Pf6N2E2_2036 0.78 +0.3 2,425,718 + -2.3 2,425,726 - +1.9 2,425,726 - +1.9 2,425,915 - Pf6N2E2_2037 0.12 +0.3 2,425,915 - Pf6N2E2_2037 0.12 +1.1 2,426,229 + Pf6N2E2_2037 0.47 -3.0 2,426,241 + Pf6N2E2_2037 0.48 -0.7 2,426,241 + Pf6N2E2_2037 0.48 -0.1 2,426,241 + Pf6N2E2_2037 0.48 -0.3 2,426,241 + Pf6N2E2_2037 0.48 +1.9 2,426,241 + Pf6N2E2_2037 0.48 +0.1 2,426,242 - Pf6N2E2_2037 0.48 +1.9 2,426,242 - Pf6N2E2_2037 0.48 +0.1 2,426,242 - Pf6N2E2_2037 0.48 -0.4 2,426,348 - Pf6N2E2_2037 0.60 -0.5 2,426,386 - Pf6N2E2_2037 0.64 +1.7 2,426,386 - Pf6N2E2_2037 0.64 -2.6 2,426,386 - Pf6N2E2_2037 0.64 +0.9 2,426,386 - Pf6N2E2_2037 0.64 -0.7 2,426,386 - Pf6N2E2_2037 0.64 +0.5 2,426,677 + -0.4
Or see this region's nucleotide sequence