Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1989

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1986 and Pf6N2E2_1987 are separated by 338 nucleotidesPf6N2E2_1987 and Pf6N2E2_1988 overlap by 4 nucleotidesPf6N2E2_1988 and Pf6N2E2_1989 are separated by 118 nucleotidesPf6N2E2_1989 and Pf6N2E2_1990 are separated by 404 nucleotides Pf6N2E2_1986 - putative membrane protein, at 2,353,114 to 2,353,863 _1986 Pf6N2E2_1987 - hypothetical protein, at 2,354,202 to 2,354,321 _1987 Pf6N2E2_1988 - hypothetical protein, at 2,354,318 to 2,354,551 _1988 Pf6N2E2_1989 - hypothetical protein, at 2,354,670 to 2,354,927 _1989 Pf6N2E2_1990 - Possible exported protein, at 2,355,332 to 2,356,015 _1990 Position (kb) 2354 2355Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2353.693 kb on + strand, within Pf6N2E2_1986at 2353.797 kb on - strandat 2353.856 kb on - strandat 2353.879 kb on + strandat 2353.959 kb on + strandat 2354.041 kb on + strandat 2354.084 kb on + strandat 2354.085 kb on - strandat 2354.092 kb on + strandat 2354.187 kb on + strandat 2354.188 kb on - strandat 2354.283 kb on + strand, within Pf6N2E2_1987at 2354.284 kb on - strand, within Pf6N2E2_1987at 2354.519 kb on + strand, within Pf6N2E2_1988at 2354.724 kb on + strand, within Pf6N2E2_1989at 2354.725 kb on - strand, within Pf6N2E2_1989at 2354.725 kb on - strand, within Pf6N2E2_1989at 2354.800 kb on - strand, within Pf6N2E2_1989at 2355.140 kb on - strandat 2355.252 kb on + strandat 2355.294 kb on - strandat 2355.294 kb on - strandat 2355.294 kb on - strandat 2355.460 kb on - strand, within Pf6N2E2_1990at 2355.503 kb on - strand, within Pf6N2E2_1990at 2355.548 kb on + strand, within Pf6N2E2_1990at 2355.549 kb on - strand, within Pf6N2E2_1990at 2355.596 kb on + strand, within Pf6N2E2_1990at 2355.596 kb on + strand, within Pf6N2E2_1990at 2355.627 kb on - strand, within Pf6N2E2_1990at 2355.665 kb on + strand, within Pf6N2E2_1990at 2355.665 kb on + strand, within Pf6N2E2_1990at 2355.684 kb on + strand, within Pf6N2E2_1990at 2355.684 kb on + strand, within Pf6N2E2_1990at 2355.685 kb on - strand, within Pf6N2E2_1990at 2355.717 kb on - strand, within Pf6N2E2_1990at 2355.717 kb on - strand, within Pf6N2E2_1990at 2355.717 kb on - strand, within Pf6N2E2_1990at 2355.769 kb on - strand, within Pf6N2E2_1990at 2355.793 kb on + strand, within Pf6N2E2_1990at 2355.873 kb on + strand, within Pf6N2E2_1990

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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2,353,693 + Pf6N2E2_1986 0.77 +0.6
2,353,797 - -1.3
2,353,856 - +0.9
2,353,879 + -1.0
2,353,959 + -3.2
2,354,041 + +0.1
2,354,084 + +0.2
2,354,085 - -2.1
2,354,092 + +0.9
2,354,187 + -1.1
2,354,188 - -0.7
2,354,283 + Pf6N2E2_1987 0.68 -0.9
2,354,284 - Pf6N2E2_1987 0.68 +1.9
2,354,519 + Pf6N2E2_1988 0.86 -1.0
2,354,724 + Pf6N2E2_1989 0.21 -0.9
2,354,725 - Pf6N2E2_1989 0.21 -1.9
2,354,725 - Pf6N2E2_1989 0.21 +0.3
2,354,800 - Pf6N2E2_1989 0.50 -2.3
2,355,140 - +1.9
2,355,252 + +3.0
2,355,294 - +0.9
2,355,294 - -1.1
2,355,294 - +0.4
2,355,460 - Pf6N2E2_1990 0.19 -1.9
2,355,503 - Pf6N2E2_1990 0.25 +1.9
2,355,548 + Pf6N2E2_1990 0.32 -1.3
2,355,549 - Pf6N2E2_1990 0.32 -0.4
2,355,596 + Pf6N2E2_1990 0.39 +1.9
2,355,596 + Pf6N2E2_1990 0.39 +1.1
2,355,627 - Pf6N2E2_1990 0.43 +0.6
2,355,665 + Pf6N2E2_1990 0.49 +0.3
2,355,665 + Pf6N2E2_1990 0.49 -0.8
2,355,684 + Pf6N2E2_1990 0.51 -0.9
2,355,684 + Pf6N2E2_1990 0.51 -3.6
2,355,685 - Pf6N2E2_1990 0.52 +0.4
2,355,717 - Pf6N2E2_1990 0.56 +1.6
2,355,717 - Pf6N2E2_1990 0.56 -2.0
2,355,717 - Pf6N2E2_1990 0.56 -0.2
2,355,769 - Pf6N2E2_1990 0.64 +1.9
2,355,793 + Pf6N2E2_1990 0.67 -2.0
2,355,873 + Pf6N2E2_1990 0.79 +1.9

Or see this region's nucleotide sequence