Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1801 and Pf6N2E2_1802 are separated by 150 nucleotides Pf6N2E2_1802 and Pf6N2E2_1803 overlap by 8 nucleotides Pf6N2E2_1803 and Pf6N2E2_1804 overlap by 4 nucleotides
Pf6N2E2_1801 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 2,130,740 to 2,131,564
_1801
Pf6N2E2_1802 - polysaccharide deacetylase, at 2,131,715 to 2,132,563
_1802
Pf6N2E2_1803 - Polysaccharide deacetylase, at 2,132,556 to 2,133,443
_1803
Pf6N2E2_1804 - Immune-responsive protein 1, at 2,133,440 to 2,134,834
_1804
Position (kb)
2132
2133
2134 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2131.678 kb on + strand at 2131.678 kb on + strand at 2131.679 kb on - strand at 2131.687 kb on + strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.751 kb on + strand at 2131.751 kb on + strand at 2131.752 kb on - strand at 2131.838 kb on + strand, within Pf6N2E2_1802 at 2131.945 kb on - strand, within Pf6N2E2_1802 at 2132.176 kb on + strand, within Pf6N2E2_1802 at 2132.177 kb on - strand, within Pf6N2E2_1802 at 2132.181 kb on - strand, within Pf6N2E2_1802 at 2132.214 kb on - strand, within Pf6N2E2_1802 at 2132.282 kb on + strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.403 kb on + strand, within Pf6N2E2_1802 at 2132.502 kb on + strand at 2133.114 kb on + strand, within Pf6N2E2_1803 at 2133.115 kb on - strand, within Pf6N2E2_1803 at 2133.172 kb on - strand, within Pf6N2E2_1803 at 2133.234 kb on + strand, within Pf6N2E2_1803 at 2133.235 kb on - strand, within Pf6N2E2_1803 at 2133.440 kb on - strand at 2133.541 kb on + strand at 2134.345 kb on - strand, within Pf6N2E2_1804 at 2134.424 kb on + strand, within Pf6N2E2_1804 at 2134.425 kb on - strand, within Pf6N2E2_1804
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 2,131,678 + +0.0 2,131,678 + -2.2 2,131,679 - +0.8 2,131,687 + -0.4 2,131,688 - +0.5 2,131,688 - -0.1 2,131,688 - +0.1 2,131,751 + +0.2 2,131,751 + +1.7 2,131,752 - -2.5 2,131,838 + Pf6N2E2_1802 0.14 -0.3 2,131,945 - Pf6N2E2_1802 0.27 +0.1 2,132,176 + Pf6N2E2_1802 0.54 +1.8 2,132,177 - Pf6N2E2_1802 0.54 +1.8 2,132,181 - Pf6N2E2_1802 0.55 -0.6 2,132,214 - Pf6N2E2_1802 0.59 +0.6 2,132,282 + Pf6N2E2_1802 0.67 +0.3 2,132,298 - Pf6N2E2_1802 0.69 +0.9 2,132,298 - Pf6N2E2_1802 0.69 -1.5 2,132,403 + Pf6N2E2_1802 0.81 -0.2 2,132,502 + -0.6 2,133,114 + Pf6N2E2_1803 0.63 -1.6 2,133,115 - Pf6N2E2_1803 0.63 -1.1 2,133,172 - Pf6N2E2_1803 0.69 -0.2 2,133,234 + Pf6N2E2_1803 0.76 -2.1 2,133,235 - Pf6N2E2_1803 0.76 -0.2 2,133,440 - -0.1 2,133,541 + +0.4 2,134,345 - Pf6N2E2_1804 0.65 +1.8 2,134,424 + Pf6N2E2_1804 0.71 -1.7 2,134,425 - Pf6N2E2_1804 0.71 -0.3
Or see this region's nucleotide sequence