Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1002 and Pf6N2E2_1003 are separated by 68 nucleotides Pf6N2E2_1003 and Pf6N2E2_1004 are separated by 88 nucleotides Pf6N2E2_1004 and Pf6N2E2_1005 are separated by 85 nucleotides
Pf6N2E2_1002 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 1,171,243 to 1,172,244
_1002
Pf6N2E2_1003 - Fucose permease, at 1,172,313 to 1,173,695
_1003
Pf6N2E2_1004 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,173,784 to 1,174,542
_1004
Pf6N2E2_1005 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,174,628 to 1,175,581
_1005
Position (kb)
1172
1173
1174 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1171.334 kb on + strand at 1171.334 kb on + strand at 1171.335 kb on - strand at 1171.396 kb on + strand, within Pf6N2E2_1002 at 1171.396 kb on + strand, within Pf6N2E2_1002 at 1171.402 kb on + strand, within Pf6N2E2_1002 at 1171.402 kb on + strand, within Pf6N2E2_1002 at 1171.403 kb on - strand, within Pf6N2E2_1002 at 1171.422 kb on + strand, within Pf6N2E2_1002 at 1171.481 kb on + strand, within Pf6N2E2_1002 at 1171.509 kb on + strand, within Pf6N2E2_1002 at 1171.537 kb on + strand, within Pf6N2E2_1002 at 1171.537 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.890 kb on + strand, within Pf6N2E2_1002 at 1171.890 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.939 kb on + strand, within Pf6N2E2_1002 at 1171.939 kb on + strand, within Pf6N2E2_1002 at 1171.940 kb on - strand, within Pf6N2E2_1002 at 1172.004 kb on + strand, within Pf6N2E2_1002 at 1172.005 kb on - strand, within Pf6N2E2_1002 at 1172.005 kb on - strand, within Pf6N2E2_1002 at 1172.092 kb on + strand, within Pf6N2E2_1002 at 1172.092 kb on + strand, within Pf6N2E2_1002 at 1172.093 kb on - strand, within Pf6N2E2_1002 at 1172.093 kb on - strand, within Pf6N2E2_1002 at 1172.133 kb on + strand, within Pf6N2E2_1002 at 1172.134 kb on - strand, within Pf6N2E2_1002 at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.226 kb on - strand at 1172.243 kb on - strand at 1172.247 kb on + strand at 1172.248 kb on - strand at 1172.276 kb on - strand at 1172.276 kb on - strand at 1172.330 kb on - strand at 1172.339 kb on + strand at 1172.340 kb on - strand at 1172.444 kb on + strand at 1172.444 kb on + strand at 1172.508 kb on - strand, within Pf6N2E2_1003 at 1172.594 kb on + strand, within Pf6N2E2_1003 at 1172.594 kb on + strand, within Pf6N2E2_1003 at 1172.595 kb on - strand, within Pf6N2E2_1003 at 1172.722 kb on + strand, within Pf6N2E2_1003 at 1172.723 kb on - strand, within Pf6N2E2_1003 at 1172.775 kb on - strand, within Pf6N2E2_1003 at 1172.927 kb on - strand, within Pf6N2E2_1003 at 1173.009 kb on + strand, within Pf6N2E2_1003 at 1173.009 kb on + strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.189 kb on + strand, within Pf6N2E2_1003 at 1173.189 kb on + strand, within Pf6N2E2_1003 at 1173.190 kb on - strand, within Pf6N2E2_1003 at 1173.219 kb on + strand, within Pf6N2E2_1003 at 1173.264 kb on + strand, within Pf6N2E2_1003 at 1173.264 kb on + strand, within Pf6N2E2_1003 at 1173.264 kb on + strand, within Pf6N2E2_1003 at 1173.291 kb on + strand, within Pf6N2E2_1003 at 1173.291 kb on + strand, within Pf6N2E2_1003 at 1173.291 kb on + strand, within Pf6N2E2_1003 at 1173.292 kb on - strand, within Pf6N2E2_1003 at 1173.292 kb on - strand, within Pf6N2E2_1003 at 1173.292 kb on - strand, within Pf6N2E2_1003 at 1173.314 kb on + strand, within Pf6N2E2_1003 at 1173.314 kb on + strand, within Pf6N2E2_1003 at 1173.441 kb on + strand, within Pf6N2E2_1003 at 1173.441 kb on + strand, within Pf6N2E2_1003 at 1173.442 kb on - strand, within Pf6N2E2_1003 at 1173.462 kb on + strand, within Pf6N2E2_1003 at 1173.462 kb on + strand, within Pf6N2E2_1003 at 1173.612 kb on + strand at 1173.612 kb on + strand at 1173.613 kb on - strand at 1173.613 kb on - strand at 1173.616 kb on + strand at 1173.684 kb on - strand at 1173.722 kb on + strand at 1173.722 kb on + strand at 1173.778 kb on + strand at 1173.779 kb on - strand at 1173.816 kb on + strand at 1173.827 kb on + strand at 1173.827 kb on + strand at 1173.827 kb on + strand at 1173.828 kb on - strand at 1173.831 kb on + strand at 1173.831 kb on + strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.946 kb on + strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.958 kb on - strand, within Pf6N2E2_1004 at 1174.023 kb on - strand, within Pf6N2E2_1004 at 1174.049 kb on + strand, within Pf6N2E2_1004 at 1174.049 kb on + strand, within Pf6N2E2_1004 at 1174.074 kb on - strand, within Pf6N2E2_1004 at 1174.081 kb on + strand, within Pf6N2E2_1004 at 1174.132 kb on - strand, within Pf6N2E2_1004 at 1174.153 kb on + strand, within Pf6N2E2_1004 at 1174.313 kb on - strand, within Pf6N2E2_1004 at 1174.422 kb on + strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.466 kb on + strand, within Pf6N2E2_1004 at 1174.466 kb on + strand, within Pf6N2E2_1004 at 1174.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 1,171,334 + +0.3 1,171,334 + +1.9 1,171,335 - -0.5 1,171,396 + Pf6N2E2_1002 0.15 +0.2 1,171,396 + Pf6N2E2_1002 0.15 +0.8 1,171,402 + Pf6N2E2_1002 0.16 -0.6 1,171,402 + Pf6N2E2_1002 0.16 +0.3 1,171,403 - Pf6N2E2_1002 0.16 -0.6 1,171,422 + Pf6N2E2_1002 0.18 +1.9 1,171,481 + Pf6N2E2_1002 0.24 -0.2 1,171,509 + Pf6N2E2_1002 0.27 +0.9 1,171,537 + Pf6N2E2_1002 0.29 -0.5 1,171,537 + Pf6N2E2_1002 0.29 -0.0 1,171,758 + Pf6N2E2_1002 0.51 -1.0 1,171,758 + Pf6N2E2_1002 0.51 +1.9 1,171,758 + Pf6N2E2_1002 0.51 -0.5 1,171,758 + Pf6N2E2_1002 0.51 -0.8 1,171,890 + Pf6N2E2_1002 0.65 -1.3 1,171,890 + Pf6N2E2_1002 0.65 +0.2 1,171,937 + Pf6N2E2_1002 0.69 +0.1 1,171,937 + Pf6N2E2_1002 0.69 +1.9 1,171,937 + Pf6N2E2_1002 0.69 -1.7 1,171,937 + Pf6N2E2_1002 0.69 -0.5 1,171,937 + Pf6N2E2_1002 0.69 -0.5 1,171,938 - Pf6N2E2_1002 0.69 -0.6 1,171,938 - Pf6N2E2_1002 0.69 +0.0 1,171,938 - -0.1 1,171,938 - Pf6N2E2_1002 0.69 +1.5 1,171,938 - Pf6N2E2_1002 0.69 +1.9 1,171,938 - Pf6N2E2_1002 0.69 -2.7 1,171,938 - Pf6N2E2_1002 0.69 +0.3 1,171,938 - Pf6N2E2_1002 0.69 +1.9 1,171,939 + Pf6N2E2_1002 0.69 -0.1 1,171,939 + Pf6N2E2_1002 0.69 +0.7 1,171,940 - Pf6N2E2_1002 0.70 -0.7 1,172,004 + Pf6N2E2_1002 0.76 -0.1 1,172,005 - Pf6N2E2_1002 0.76 -0.1 1,172,005 - Pf6N2E2_1002 0.76 -1.9 1,172,092 + Pf6N2E2_1002 0.85 -2.1 1,172,092 + Pf6N2E2_1002 0.85 +0.1 1,172,093 - Pf6N2E2_1002 0.85 +0.5 1,172,093 - Pf6N2E2_1002 0.85 -1.0 1,172,133 + Pf6N2E2_1002 0.89 -0.0 1,172,134 - Pf6N2E2_1002 0.89 +0.9 1,172,225 + -0.6 1,172,225 + +0.2 1,172,225 + -0.6 1,172,225 + -0.8 1,172,225 + -1.8 1,172,226 - +0.3 1,172,243 - +1.9 1,172,247 + -1.2 1,172,248 - +0.9 1,172,276 - +0.2 1,172,276 - -0.1 1,172,330 - +1.9 1,172,339 + +2.2 1,172,340 - -2.9 1,172,444 + -0.5 1,172,444 + -1.3 1,172,508 - Pf6N2E2_1003 0.14 -1.3 1,172,594 + Pf6N2E2_1003 0.20 -0.4 1,172,594 + Pf6N2E2_1003 0.20 -1.7 1,172,595 - Pf6N2E2_1003 0.20 +0.5 1,172,722 + Pf6N2E2_1003 0.30 -0.7 1,172,723 - Pf6N2E2_1003 0.30 +1.9 1,172,775 - Pf6N2E2_1003 0.33 -2.1 1,172,927 - Pf6N2E2_1003 0.44 +1.9 1,173,009 + Pf6N2E2_1003 0.50 +1.9 1,173,009 + Pf6N2E2_1003 0.50 -0.5 1,173,010 - Pf6N2E2_1003 0.50 +0.2 1,173,010 - Pf6N2E2_1003 0.50 -1.2 1,173,010 - Pf6N2E2_1003 0.50 +0.7 1,173,125 - Pf6N2E2_1003 0.59 -1.5 1,173,125 - Pf6N2E2_1003 0.59 -0.9 1,173,125 - Pf6N2E2_1003 0.59 +2.2 1,173,189 + Pf6N2E2_1003 0.63 -1.0 1,173,189 + Pf6N2E2_1003 0.63 +1.9 1,173,190 - Pf6N2E2_1003 0.63 +1.9 1,173,219 + Pf6N2E2_1003 0.66 +0.5 1,173,264 + Pf6N2E2_1003 0.69 -0.5 1,173,264 + Pf6N2E2_1003 0.69 +0.3 1,173,264 + Pf6N2E2_1003 0.69 -0.5 1,173,291 + Pf6N2E2_1003 0.71 -2.7 1,173,291 + Pf6N2E2_1003 0.71 -0.6 1,173,291 + Pf6N2E2_1003 0.71 -1.6 1,173,292 - Pf6N2E2_1003 0.71 -0.7 1,173,292 - Pf6N2E2_1003 0.71 -1.2 1,173,292 - Pf6N2E2_1003 0.71 -1.6 1,173,314 + Pf6N2E2_1003 0.72 +1.9 1,173,314 + Pf6N2E2_1003 0.72 +0.6 1,173,441 + Pf6N2E2_1003 0.82 +1.9 1,173,441 + Pf6N2E2_1003 0.82 +0.2 1,173,442 - Pf6N2E2_1003 0.82 -3.4 1,173,462 + Pf6N2E2_1003 0.83 +1.9 1,173,462 + Pf6N2E2_1003 0.83 -0.1 1,173,612 + -0.2 1,173,612 + -0.6 1,173,613 - +1.9 1,173,613 - -0.5 1,173,616 + -0.1 1,173,684 - -0.6 1,173,722 + +0.9 1,173,722 + -0.5 1,173,778 + +0.9 1,173,779 - -0.0 1,173,816 + -0.7 1,173,827 + +0.9 1,173,827 + -1.0 1,173,827 + -0.2 1,173,828 - -2.3 1,173,831 + +0.9 1,173,831 + -0.4 1,173,832 - +0.9 1,173,832 - +0.6 1,173,832 - -3.2 1,173,832 - +1.9 1,173,832 - +2.9 1,173,946 + Pf6N2E2_1004 0.21 +1.9 1,173,947 - Pf6N2E2_1004 0.21 -1.3 1,173,947 - Pf6N2E2_1004 0.21 +0.9 1,173,947 - Pf6N2E2_1004 0.21 +0.1 1,173,958 - Pf6N2E2_1004 0.23 +0.4 1,174,023 - Pf6N2E2_1004 0.31 +0.9 1,174,049 + Pf6N2E2_1004 0.35 -1.9 1,174,049 + Pf6N2E2_1004 0.35 +0.9 1,174,074 - Pf6N2E2_1004 0.38 -1.1 1,174,081 + Pf6N2E2_1004 0.39 +0.9 1,174,132 - Pf6N2E2_1004 0.46 +0.9 1,174,153 + Pf6N2E2_1004 0.49 +0.4 1,174,313 - Pf6N2E2_1004 0.70 +1.9 1,174,422 + Pf6N2E2_1004 0.84 -2.0 1,174,423 - Pf6N2E2_1004 0.84 +1.9 1,174,423 - Pf6N2E2_1004 0.84 -0.9 1,174,423 - Pf6N2E2_1004 0.84 +0.8 1,174,466 + Pf6N2E2_1004 0.90 -1.5 1,174,466 + Pf6N2E2_1004 0.90 -0.7 1,174,693 - +1.9
Or see this region's nucleotide sequence