Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_591 and Pf6N2E2_592 overlap by 35 nucleotides Pf6N2E2_592 and Pf6N2E2_593 are separated by 86 nucleotides Pf6N2E2_593 and Pf6N2E2_594 are separated by 203 nucleotides Pf6N2E2_594 and Pf6N2E2_595 are separated by 197 nucleotides
Pf6N2E2_591 - Streptothricin acetyltransferase, Streptomyces lavendulae type, at 668,446 to 669,060
_591
Pf6N2E2_592 - Transcriptional regulators, LysR family, at 669,026 to 669,919
_592
Pf6N2E2_593 - FIG00958166: hypothetical protein, at 670,006 to 670,269
_593
Pf6N2E2_594 - FMN reductase, NADPH-dependent, at 670,473 to 671,012
_594
Pf6N2E2_595 - Arsenical resistance operon repressor, at 671,210 to 671,560
_595
Position (kb) 670
671 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 669.121 kb on - strand, within Pf6N2E2_592 at 669.302 kb on - strand, within Pf6N2E2_592 at 669.344 kb on + strand, within Pf6N2E2_592 at 669.345 kb on - strand, within Pf6N2E2_592 at 669.345 kb on - strand, within Pf6N2E2_592 at 669.382 kb on - strand, within Pf6N2E2_592 at 669.382 kb on - strand, within Pf6N2E2_592 at 669.382 kb on - strand, within Pf6N2E2_592 at 669.634 kb on + strand, within Pf6N2E2_592 at 669.635 kb on - strand, within Pf6N2E2_592 at 669.657 kb on + strand, within Pf6N2E2_592 at 669.705 kb on + strand, within Pf6N2E2_592 at 669.912 kb on - strand at 669.997 kb on - strand at 670.015 kb on + strand at 670.143 kb on + strand, within Pf6N2E2_593 at 670.143 kb on + strand, within Pf6N2E2_593 at 670.144 kb on - strand, within Pf6N2E2_593 at 670.144 kb on - strand, within Pf6N2E2_593 at 670.144 kb on - strand, within Pf6N2E2_593 at 670.145 kb on + strand, within Pf6N2E2_593 at 670.145 kb on + strand, within Pf6N2E2_593 at 670.145 kb on + strand, within Pf6N2E2_593 at 670.146 kb on - strand, within Pf6N2E2_593 at 670.146 kb on - strand, within Pf6N2E2_593 at 670.146 kb on - strand, within Pf6N2E2_593 at 670.259 kb on - strand at 670.275 kb on - strand at 670.343 kb on + strand at 670.344 kb on - strand at 670.412 kb on + strand at 670.412 kb on + strand at 670.412 kb on + strand at 670.412 kb on + strand at 670.412 kb on + strand at 670.412 kb on + strand at 670.413 kb on - strand at 670.413 kb on - strand at 670.414 kb on + strand at 670.414 kb on + strand at 670.415 kb on - strand at 670.415 kb on - strand at 670.415 kb on - strand at 670.418 kb on + strand at 670.418 kb on + strand at 670.418 kb on + strand at 670.418 kb on + strand at 670.418 kb on + strand at 670.419 kb on - strand at 670.460 kb on + strand at 670.460 kb on + strand at 670.460 kb on + strand at 670.461 kb on - strand at 670.461 kb on - strand at 670.461 kb on - strand at 670.467 kb on + strand at 670.596 kb on + strand, within Pf6N2E2_594 at 670.597 kb on - strand, within Pf6N2E2_594 at 670.597 kb on - strand, within Pf6N2E2_594 at 670.597 kb on - strand, within Pf6N2E2_594 at 670.597 kb on - strand, within Pf6N2E2_594 at 670.597 kb on - strand, within Pf6N2E2_594 at 670.598 kb on + strand, within Pf6N2E2_594 at 670.598 kb on + strand, within Pf6N2E2_594 at 670.599 kb on - strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.632 kb on + strand, within Pf6N2E2_594 at 670.632 kb on + strand, within Pf6N2E2_594 at 670.632 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.693 kb on - strand, within Pf6N2E2_594 at 670.968 kb on - strand at 670.968 kb on - strand at 671.031 kb on - strand at 671.173 kb on - strand at 671.174 kb on + strand at 671.175 kb on - strand at 671.175 kb on - strand at 671.188 kb on + strand at 671.206 kb on - strand at 671.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 669,121 - Pf6N2E2_592 0.11 +2.7 669,302 - Pf6N2E2_592 0.31 +1.2 669,344 + Pf6N2E2_592 0.36 -0.1 669,345 - Pf6N2E2_592 0.36 -0.7 669,345 - Pf6N2E2_592 0.36 +0.5 669,382 - Pf6N2E2_592 0.40 -0.2 669,382 - Pf6N2E2_592 0.40 +0.8 669,382 - Pf6N2E2_592 0.40 +0.6 669,634 + Pf6N2E2_592 0.68 -0.7 669,635 - Pf6N2E2_592 0.68 +0.9 669,657 + Pf6N2E2_592 0.71 -0.4 669,705 + Pf6N2E2_592 0.76 -1.0 669,912 - +1.9 669,997 - -0.9 670,015 + +1.9 670,143 + Pf6N2E2_593 0.52 -3.5 670,143 + Pf6N2E2_593 0.52 +1.9 670,144 - Pf6N2E2_593 0.52 +1.9 670,144 - Pf6N2E2_593 0.52 -2.9 670,144 - Pf6N2E2_593 0.52 -1.3 670,145 + Pf6N2E2_593 0.53 +1.9 670,145 + Pf6N2E2_593 0.53 -0.1 670,145 + Pf6N2E2_593 0.53 +0.9 670,146 - Pf6N2E2_593 0.53 +0.2 670,146 - Pf6N2E2_593 0.53 -1.3 670,146 - Pf6N2E2_593 0.53 -1.1 670,259 - +1.9 670,275 - +0.3 670,343 + -0.1 670,344 - +0.3 670,412 + -2.3 670,412 + +1.9 670,412 + +1.9 670,412 + +0.3 670,412 + +1.9 670,412 + +0.3 670,413 - +0.9 670,413 - -2.1 670,414 + -0.8 670,414 + -1.8 670,415 - +0.1 670,415 - +0.9 670,415 - -1.4 670,418 + +1.9 670,418 + -2.3 670,418 + -0.3 670,418 + +2.5 670,418 + +0.9 670,419 - -1.4 670,460 + -1.1 670,460 + +0.4 670,460 + +1.9 670,461 - -1.3 670,461 - +1.9 670,461 - +1.9 670,467 + -0.3 670,596 + Pf6N2E2_594 0.23 +1.9 670,597 - Pf6N2E2_594 0.23 -1.3 670,597 - Pf6N2E2_594 0.23 +0.3 670,597 - Pf6N2E2_594 0.23 +1.3 670,597 - Pf6N2E2_594 0.23 +1.9 670,597 - Pf6N2E2_594 0.23 -0.1 670,598 + Pf6N2E2_594 0.23 -0.3 670,598 + Pf6N2E2_594 0.23 -0.5 670,599 - Pf6N2E2_594 0.23 -0.3 670,615 + Pf6N2E2_594 0.26 -1.0 670,615 + Pf6N2E2_594 0.26 -0.1 670,615 + Pf6N2E2_594 0.26 -1.7 670,615 + Pf6N2E2_594 0.26 +1.9 670,632 + Pf6N2E2_594 0.29 +1.9 670,632 + Pf6N2E2_594 0.29 +1.9 670,632 + Pf6N2E2_594 0.29 -2.2 670,692 + Pf6N2E2_594 0.41 +1.9 670,692 + Pf6N2E2_594 0.41 -2.6 670,692 + Pf6N2E2_594 0.41 +0.7 670,692 + Pf6N2E2_594 0.41 +1.5 670,693 - Pf6N2E2_594 0.41 +0.1 670,968 - -0.9 670,968 - +0.3 671,031 - -0.5 671,173 - +0.0 671,174 + -0.2 671,175 - -0.1 671,175 - -0.2 671,188 + -1.1 671,206 - +0.7 671,206 - +0.5
Or see this region's nucleotide sequence