Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_31 and Pf6N2E2_32 are separated by 204 nucleotides Pf6N2E2_32 and Pf6N2E2_33 are separated by 179 nucleotides
Pf6N2E2_31 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 68,024 to 70,462
_31
Pf6N2E2_32 - Membrane protein mosC, at 70,667 to 71,818
_32
Pf6N2E2_33 - Diaminopimelate epimerase (EC 5.1.1.7), at 71,998 to 72,999
_33
Position (kb)
70
71
72 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.686 kb on + strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.687 kb on - strand, within Pf6N2E2_31 at 69.722 kb on + strand, within Pf6N2E2_31 at 69.723 kb on - strand, within Pf6N2E2_31 at 69.723 kb on - strand, within Pf6N2E2_31 at 69.749 kb on + strand, within Pf6N2E2_31 at 69.821 kb on + strand, within Pf6N2E2_31 at 69.822 kb on - strand, within Pf6N2E2_31 at 69.839 kb on + strand, within Pf6N2E2_31 at 69.840 kb on - strand, within Pf6N2E2_31 at 69.878 kb on + strand, within Pf6N2E2_31 at 69.878 kb on + strand, within Pf6N2E2_31 at 69.879 kb on - strand, within Pf6N2E2_31 at 69.879 kb on - strand, within Pf6N2E2_31 at 69.906 kb on - strand, within Pf6N2E2_31 at 69.906 kb on - strand, within Pf6N2E2_31 at 69.978 kb on - strand, within Pf6N2E2_31 at 69.978 kb on - strand, within Pf6N2E2_31 at 70.038 kb on - strand, within Pf6N2E2_31 at 70.052 kb on + strand, within Pf6N2E2_31 at 70.052 kb on + strand, within Pf6N2E2_31 at 70.052 kb on + strand, within Pf6N2E2_31 at 70.052 kb on + strand, within Pf6N2E2_31 at 70.053 kb on - strand, within Pf6N2E2_31 at 70.053 kb on - strand, within Pf6N2E2_31 at 70.097 kb on + strand, within Pf6N2E2_31 at 70.098 kb on - strand, within Pf6N2E2_31 at 70.130 kb on + strand, within Pf6N2E2_31 at 70.151 kb on + strand, within Pf6N2E2_31 at 70.151 kb on + strand, within Pf6N2E2_31 at 70.151 kb on + strand, within Pf6N2E2_31 at 70.152 kb on - strand, within Pf6N2E2_31 at 70.152 kb on - strand, within Pf6N2E2_31 at 70.152 kb on - strand, within Pf6N2E2_31 at 70.232 kb on + strand at 70.232 kb on + strand at 70.232 kb on + strand at 70.233 kb on - strand at 70.233 kb on - strand at 70.233 kb on - strand at 70.233 kb on - strand at 70.233 kb on - strand at 70.280 kb on + strand at 70.307 kb on + strand at 70.376 kb on + strand at 70.376 kb on + strand at 70.377 kb on - strand at 70.377 kb on - strand at 70.379 kb on + strand at 70.379 kb on + strand at 70.379 kb on + strand at 70.379 kb on + strand at 70.380 kb on - strand at 70.380 kb on - strand at 70.380 kb on - strand at 70.380 kb on - strand at 70.380 kb on - strand at 70.380 kb on - strand at 70.412 kb on + strand at 70.412 kb on + strand at 70.413 kb on - strand at 70.413 kb on - strand at 70.413 kb on - strand at 70.429 kb on + strand at 70.480 kb on + strand at 70.480 kb on + strand at 70.480 kb on + strand at 70.481 kb on - strand at 70.498 kb on + strand at 70.498 kb on + strand at 70.511 kb on - strand at 70.841 kb on + strand, within Pf6N2E2_32 at 70.842 kb on - strand, within Pf6N2E2_32 at 70.842 kb on - strand, within Pf6N2E2_32 at 70.866 kb on + strand, within Pf6N2E2_32 at 70.866 kb on + strand, within Pf6N2E2_32 at 70.920 kb on - strand, within Pf6N2E2_32 at 70.920 kb on - strand, within Pf6N2E2_32 at 70.986 kb on + strand, within Pf6N2E2_32 at 70.987 kb on - strand, within Pf6N2E2_32 at 70.987 kb on - strand, within Pf6N2E2_32 at 71.018 kb on + strand, within Pf6N2E2_32 at 71.018 kb on + strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.073 kb on + strand, within Pf6N2E2_32 at 71.282 kb on + strand, within Pf6N2E2_32 at 71.283 kb on - strand, within Pf6N2E2_32 at 71.834 kb on + strand at 71.835 kb on - strand at 71.865 kb on + strand at 71.865 kb on + strand at 71.866 kb on - strand at 71.866 kb on - strand at 71.866 kb on - strand at 71.959 kb on + strand at 71.959 kb on + strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.964 kb on + strand at 72.011 kb on - strand at 72.011 kb on - strand at 72.027 kb on + strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.032 kb on - strand at 72.032 kb on - strand at 72.255 kb on + strand, within Pf6N2E2_33 at 72.694 kb on + strand, within Pf6N2E2_33 at 72.695 kb on - strand, within Pf6N2E2_33
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 69,686 + Pf6N2E2_31 0.68 -0.6 69,686 + Pf6N2E2_31 0.68 -1.7 69,686 + Pf6N2E2_31 0.68 +1.1 69,686 + Pf6N2E2_31 0.68 -0.0 69,686 + Pf6N2E2_31 0.68 -0.4 69,686 + Pf6N2E2_31 0.68 -0.6 69,686 + Pf6N2E2_31 0.68 +2.0 69,686 + Pf6N2E2_31 0.68 +0.0 69,686 + Pf6N2E2_31 0.68 -0.6 69,687 - Pf6N2E2_31 0.68 +2.0 69,687 - Pf6N2E2_31 0.68 +0.5 69,687 - Pf6N2E2_31 0.68 -0.8 69,687 - Pf6N2E2_31 0.68 +0.0 69,687 - Pf6N2E2_31 0.68 -0.5 69,687 - Pf6N2E2_31 0.68 +0.3 69,687 - Pf6N2E2_31 0.68 -1.1 69,687 - Pf6N2E2_31 0.68 -2.0 69,687 - Pf6N2E2_31 0.68 +0.6 69,687 - Pf6N2E2_31 0.68 +2.0 69,687 - Pf6N2E2_31 0.68 +0.2 69,687 - Pf6N2E2_31 0.68 -2.0 69,722 + Pf6N2E2_31 0.70 +0.2 69,723 - Pf6N2E2_31 0.70 +0.3 69,723 - Pf6N2E2_31 0.70 +1.0 69,749 + Pf6N2E2_31 0.71 +1.5 69,821 + Pf6N2E2_31 0.74 -1.3 69,822 - Pf6N2E2_31 0.74 -1.2 69,839 + Pf6N2E2_31 0.74 +0.0 69,840 - Pf6N2E2_31 0.74 -0.0 69,878 + Pf6N2E2_31 0.76 +0.8 69,878 + Pf6N2E2_31 0.76 -0.5 69,879 - Pf6N2E2_31 0.76 +0.7 69,879 - Pf6N2E2_31 0.76 +1.0 69,906 - Pf6N2E2_31 0.77 +1.1 69,906 - Pf6N2E2_31 0.77 +0.0 69,978 - Pf6N2E2_31 0.80 +0.4 69,978 - Pf6N2E2_31 0.80 +0.0 70,038 - Pf6N2E2_31 0.83 -0.8 70,052 + Pf6N2E2_31 0.83 -0.7 70,052 + Pf6N2E2_31 0.83 -0.5 70,052 + Pf6N2E2_31 0.83 +0.7 70,052 + Pf6N2E2_31 0.83 -0.9 70,053 - Pf6N2E2_31 0.83 -1.9 70,053 - Pf6N2E2_31 0.83 +0.0 70,097 + Pf6N2E2_31 0.85 +1.6 70,098 - Pf6N2E2_31 0.85 -0.6 70,130 + Pf6N2E2_31 0.86 +2.0 70,151 + Pf6N2E2_31 0.87 -2.1 70,151 + Pf6N2E2_31 0.87 -1.0 70,151 + Pf6N2E2_31 0.87 +1.3 70,152 - Pf6N2E2_31 0.87 +0.5 70,152 - Pf6N2E2_31 0.87 -1.8 70,152 - Pf6N2E2_31 0.87 -0.6 70,232 + -0.6 70,232 + +0.2 70,232 + +2.5 70,233 - -2.4 70,233 - -0.5 70,233 - -1.8 70,233 - -0.3 70,233 - +0.2 70,280 + +0.1 70,307 + -0.4 70,376 + -2.6 70,376 + +1.3 70,377 - +0.2 70,377 - -0.2 70,379 + -0.6 70,379 + +0.8 70,379 + -0.3 70,379 + +0.3 70,380 - -2.1 70,380 - -0.9 70,380 - +1.1 70,380 - +0.5 70,380 - +1.1 70,380 - -3.9 70,412 + -0.2 70,412 + -1.3 70,413 - +0.0 70,413 - +1.4 70,413 - +2.0 70,429 + +0.2 70,480 + +1.7 70,480 + +0.1 70,480 + +1.0 70,481 - -0.3 70,498 + +0.0 70,498 + -3.8 70,511 - +1.7 70,841 + Pf6N2E2_32 0.15 +0.9 70,842 - Pf6N2E2_32 0.15 -0.2 70,842 - Pf6N2E2_32 0.15 -0.4 70,866 + Pf6N2E2_32 0.17 +1.7 70,866 + Pf6N2E2_32 0.17 -3.1 70,920 - Pf6N2E2_32 0.22 +0.2 70,920 - Pf6N2E2_32 0.22 +0.4 70,986 + Pf6N2E2_32 0.28 +1.8 70,987 - Pf6N2E2_32 0.28 -2.6 70,987 - Pf6N2E2_32 0.28 +0.4 71,018 + Pf6N2E2_32 0.30 -1.1 71,018 + Pf6N2E2_32 0.30 +0.9 71,019 - Pf6N2E2_32 0.31 +1.2 71,019 - Pf6N2E2_32 0.31 -0.2 71,019 - Pf6N2E2_32 0.31 +0.8 71,019 - Pf6N2E2_32 0.31 +0.5 71,073 + Pf6N2E2_32 0.35 -0.8 71,282 + Pf6N2E2_32 0.53 +2.0 71,283 - Pf6N2E2_32 0.53 +0.7 71,834 + -0.1 71,835 - +2.2 71,865 + -0.2 71,865 + +0.5 71,866 - -0.6 71,866 - -0.8 71,866 - -0.2 71,959 + +1.0 71,959 + -0.1 71,960 - +0.7 71,960 - +0.5 71,960 - -2.3 71,960 - -0.3 71,960 - +0.1 71,964 + -1.6 72,011 - +2.0 72,011 - -0.1 72,027 + -0.0 72,028 - -1.9 72,028 - -2.3 72,028 - +2.0 72,028 - -2.9 72,028 - +0.2 72,028 - +1.0 72,028 - -1.1 72,028 - -0.6 72,031 + -0.2 72,031 + +0.0 72,031 + -0.5 72,031 + +0.9 72,032 - +1.0 72,032 - -1.3 72,255 + Pf6N2E2_33 0.26 -1.0 72,694 + Pf6N2E2_33 0.69 +0.9 72,695 - Pf6N2E2_33 0.70 +1.0
Or see this region's nucleotide sequence