Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_244

Experiment: nitrogen source nitrate 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_242 and Pf6N2E2_243 are separated by 49 nucleotidesPf6N2E2_243 and Pf6N2E2_244 are separated by 36 nucleotidesPf6N2E2_244 and Pf6N2E2_245 are separated by 37 nucleotidesPf6N2E2_245 and Pf6N2E2_246 are separated by 36 nucleotides Pf6N2E2_242 - Glycine betaine ABC transport system permease protein, at 302,519 to 303,202 _242 Pf6N2E2_243 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 303,252 to 304,157 _243 Pf6N2E2_244 - Nitrilotriacetate monooxygenase component A (EC 1.14.13.-), at 304,194 to 305,522 _244 Pf6N2E2_245 - ABC-type phosphate/phosphonate transport system, periplasmic component, at 305,560 to 306,342 _245 Pf6N2E2_246 - Methyl-accepting chemotaxis protein, at 306,379 to 308,322 _246 Position (kb) 304 305 306Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 303.196 kb on + strandat 303.405 kb on + strand, within Pf6N2E2_243at 303.405 kb on + strand, within Pf6N2E2_243at 303.405 kb on + strand, within Pf6N2E2_243at 303.405 kb on + strand, within Pf6N2E2_243at 303.406 kb on - strand, within Pf6N2E2_243at 303.519 kb on + strand, within Pf6N2E2_243at 303.520 kb on - strand, within Pf6N2E2_243at 303.520 kb on - strand, within Pf6N2E2_243at 303.523 kb on + strand, within Pf6N2E2_243at 303.523 kb on + strand, within Pf6N2E2_243at 303.523 kb on + strand, within Pf6N2E2_243at 303.523 kb on + strand, within Pf6N2E2_243at 303.523 kb on + strand, within Pf6N2E2_243at 303.523 kb on + strand, within Pf6N2E2_243at 303.523 kb on + strand, within Pf6N2E2_243at 303.647 kb on + strand, within Pf6N2E2_243at 303.648 kb on - strand, within Pf6N2E2_243at 303.687 kb on + strand, within Pf6N2E2_243at 303.773 kb on - strand, within Pf6N2E2_243at 303.799 kb on - strand, within Pf6N2E2_243at 303.974 kb on + strand, within Pf6N2E2_243at 304.136 kb on + strandat 304.176 kb on + strandat 304.176 kb on + strandat 304.177 kb on - strandat 304.203 kb on - strandat 304.291 kb on + strandat 304.306 kb on - strandat 304.308 kb on + strandat 304.308 kb on + strandat 304.308 kb on + strandat 304.308 kb on + strandat 304.308 kb on + strandat 304.309 kb on - strandat 304.309 kb on - strandat 304.309 kb on - strandat 304.312 kb on + strandat 304.312 kb on + strandat 304.312 kb on + strandat 304.312 kb on + strandat 304.312 kb on + strandat 304.312 kb on + strandat 304.313 kb on - strandat 304.313 kb on - strandat 304.313 kb on - strandat 304.410 kb on + strand, within Pf6N2E2_244at 304.437 kb on - strand, within Pf6N2E2_244at 304.437 kb on - strand, within Pf6N2E2_244at 304.503 kb on + strand, within Pf6N2E2_244at 304.503 kb on + strand, within Pf6N2E2_244at 304.504 kb on - strand, within Pf6N2E2_244at 304.504 kb on - strand, within Pf6N2E2_244at 304.504 kb on - strand, within Pf6N2E2_244at 304.548 kb on + strand, within Pf6N2E2_244at 304.549 kb on - strand, within Pf6N2E2_244at 304.549 kb on - strand, within Pf6N2E2_244at 304.577 kb on + strand, within Pf6N2E2_244at 304.922 kb on + strand, within Pf6N2E2_244at 304.923 kb on - strand, within Pf6N2E2_244at 304.945 kb on - strand, within Pf6N2E2_244at 305.118 kb on + strand, within Pf6N2E2_244at 305.119 kb on - strand, within Pf6N2E2_244at 305.312 kb on + strand, within Pf6N2E2_244at 305.337 kb on + strand, within Pf6N2E2_244at 305.337 kb on + strand, within Pf6N2E2_244at 305.337 kb on + strand, within Pf6N2E2_244at 305.361 kb on + strand, within Pf6N2E2_244at 305.361 kb on + strand, within Pf6N2E2_244at 305.362 kb on - strand, within Pf6N2E2_244at 305.362 kb on - strand, within Pf6N2E2_244at 305.362 kb on - strand, within Pf6N2E2_244at 305.406 kb on - strandat 305.457 kb on + strandat 305.457 kb on + strandat 305.457 kb on + strandat 305.573 kb on + strandat 305.573 kb on + strandat 305.574 kb on - strandat 305.574 kb on - strandat 305.582 kb on - strandat 305.582 kb on - strandat 305.582 kb on - strandat 305.603 kb on + strandat 305.785 kb on + strand, within Pf6N2E2_245at 305.785 kb on + strand, within Pf6N2E2_245at 305.785 kb on + strand, within Pf6N2E2_245at 305.786 kb on - strand, within Pf6N2E2_245at 305.835 kb on + strand, within Pf6N2E2_245at 305.835 kb on + strand, within Pf6N2E2_245at 305.835 kb on + strand, within Pf6N2E2_245at 305.836 kb on - strand, within Pf6N2E2_245at 305.836 kb on - strand, within Pf6N2E2_245at 305.836 kb on - strand, within Pf6N2E2_245at 306.058 kb on + strand, within Pf6N2E2_245at 306.058 kb on + strand, within Pf6N2E2_245at 306.064 kb on + strand, within Pf6N2E2_245at 306.064 kb on + strand, within Pf6N2E2_245at 306.142 kb on + strand, within Pf6N2E2_245at 306.142 kb on + strand, within Pf6N2E2_245at 306.143 kb on - strand, within Pf6N2E2_245at 306.143 kb on - strand, within Pf6N2E2_245at 306.146 kb on + strand, within Pf6N2E2_245at 306.146 kb on + strand, within Pf6N2E2_245at 306.146 kb on + strand, within Pf6N2E2_245at 306.146 kb on + strand, within Pf6N2E2_245at 306.147 kb on - strand, within Pf6N2E2_245at 306.147 kb on - strand, within Pf6N2E2_245at 306.159 kb on + strand, within Pf6N2E2_245at 306.183 kb on + strand, within Pf6N2E2_245at 306.277 kb on + strandat 306.277 kb on + strandat 306.295 kb on + strandat 306.295 kb on + strandat 306.295 kb on + strandat 306.332 kb on + strandat 306.332 kb on + strandat 306.332 kb on + strandat 306.333 kb on - strandat 306.333 kb on - strandat 306.381 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM
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303,196 + +2.0
303,405 + Pf6N2E2_243 0.17 +0.8
303,405 + Pf6N2E2_243 0.17 -0.4
303,405 + Pf6N2E2_243 0.17 -0.7
303,405 + Pf6N2E2_243 0.17 +0.0
303,406 - Pf6N2E2_243 0.17 -0.6
303,519 + Pf6N2E2_243 0.29 +0.4
303,520 - Pf6N2E2_243 0.30 +2.0
303,520 - Pf6N2E2_243 0.30 +1.0
303,523 + Pf6N2E2_243 0.30 +0.7
303,523 + Pf6N2E2_243 0.30 -0.0
303,523 + Pf6N2E2_243 0.30 +0.6
303,523 + Pf6N2E2_243 0.30 -0.0
303,523 + Pf6N2E2_243 0.30 +0.6
303,523 + Pf6N2E2_243 0.30 -0.2
303,523 + Pf6N2E2_243 0.30 -1.2
303,647 + Pf6N2E2_243 0.44 -0.3
303,648 - Pf6N2E2_243 0.44 -0.8
303,687 + Pf6N2E2_243 0.48 +0.3
303,773 - Pf6N2E2_243 0.58 +0.2
303,799 - Pf6N2E2_243 0.60 +2.0
303,974 + Pf6N2E2_243 0.80 -0.3
304,136 + +0.9
304,176 + +0.3
304,176 + +2.0
304,177 - +2.0
304,203 - -0.3
304,291 + -0.3
304,306 - -0.4
304,308 + -1.2
304,308 + -1.9
304,308 + +2.0
304,308 + -0.1
304,308 + +0.6
304,309 - +0.5
304,309 - -0.8
304,309 - -0.5
304,312 + +0.0
304,312 + +0.9
304,312 + -0.0
304,312 + -0.6
304,312 + -0.6
304,312 + +0.2
304,313 - +0.4
304,313 - -2.8
304,313 - -1.7
304,410 + Pf6N2E2_244 0.16 +0.4
304,437 - Pf6N2E2_244 0.18 -0.8
304,437 - Pf6N2E2_244 0.18 -0.3
304,503 + Pf6N2E2_244 0.23 -1.3
304,503 + Pf6N2E2_244 0.23 +1.9
304,504 - Pf6N2E2_244 0.23 -0.7
304,504 - Pf6N2E2_244 0.23 +1.0
304,504 - Pf6N2E2_244 0.23 -0.7
304,548 + Pf6N2E2_244 0.27 +1.7
304,549 - Pf6N2E2_244 0.27 +2.0
304,549 - Pf6N2E2_244 0.27 +2.0
304,577 + Pf6N2E2_244 0.29 +0.1
304,922 + Pf6N2E2_244 0.55 -0.8
304,923 - Pf6N2E2_244 0.55 +2.0
304,945 - Pf6N2E2_244 0.57 -0.6
305,118 + Pf6N2E2_244 0.70 -1.9
305,119 - Pf6N2E2_244 0.70 +0.0
305,312 + Pf6N2E2_244 0.84 -0.3
305,337 + Pf6N2E2_244 0.86 -0.0
305,337 + Pf6N2E2_244 0.86 -0.8
305,337 + Pf6N2E2_244 0.86 +0.3
305,361 + Pf6N2E2_244 0.88 -0.0
305,361 + Pf6N2E2_244 0.88 -3.1
305,362 - Pf6N2E2_244 0.88 -0.5
305,362 - Pf6N2E2_244 0.88 +1.0
305,362 - Pf6N2E2_244 0.88 -0.3
305,406 - +0.7
305,457 + -1.1
305,457 + -0.8
305,457 + -0.7
305,573 + -0.1
305,573 + +2.0
305,574 - +1.4
305,574 - -0.0
305,582 - -0.6
305,582 - -2.1
305,582 - +1.0
305,603 + -0.3
305,785 + Pf6N2E2_245 0.29 +0.4
305,785 + Pf6N2E2_245 0.29 +0.4
305,785 + Pf6N2E2_245 0.29 -2.0
305,786 - Pf6N2E2_245 0.29 +0.8
305,835 + Pf6N2E2_245 0.35 +0.4
305,835 + Pf6N2E2_245 0.35 -0.2
305,835 + Pf6N2E2_245 0.35 +0.4
305,836 - Pf6N2E2_245 0.35 +0.9
305,836 - Pf6N2E2_245 0.35 +0.4
305,836 - Pf6N2E2_245 0.35 -2.5
306,058 + Pf6N2E2_245 0.64 +0.6
306,058 + Pf6N2E2_245 0.64 -0.0
306,064 + Pf6N2E2_245 0.64 -0.4
306,064 + Pf6N2E2_245 0.64 -0.4
306,142 + Pf6N2E2_245 0.74 +2.0
306,142 + Pf6N2E2_245 0.74 -0.2
306,143 - Pf6N2E2_245 0.74 +2.0
306,143 - Pf6N2E2_245 0.74 +1.7
306,146 + Pf6N2E2_245 0.75 -0.1
306,146 + Pf6N2E2_245 0.75 -0.9
306,146 + Pf6N2E2_245 0.75 +0.4
306,146 + Pf6N2E2_245 0.75 +1.0
306,147 - Pf6N2E2_245 0.75 -1.6
306,147 - Pf6N2E2_245 0.75 -0.8
306,159 + Pf6N2E2_245 0.77 +1.4
306,183 + Pf6N2E2_245 0.80 -0.2
306,277 + +0.6
306,277 + +0.7
306,295 + +2.0
306,295 + +0.8
306,295 + -0.9
306,332 + +1.3
306,332 + +0.1
306,332 + +0.5
306,333 - +0.4
306,333 - -1.3
306,381 - +0.8

Or see this region's nucleotide sequence