Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1602

Experiment: nitrogen source nitrate 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1600 and Pf6N2E2_1601 are separated by 5 nucleotidesPf6N2E2_1601 and Pf6N2E2_1602 are separated by 70 nucleotidesPf6N2E2_1602 and Pf6N2E2_1603 are separated by 163 nucleotidesPf6N2E2_1603 and Pf6N2E2_1604 are separated by 62 nucleotides Pf6N2E2_1600 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 1,872,220 to 1,873,872 _1600 Pf6N2E2_1601 - DNA-binding response regulator, LuxR family, at 1,873,878 to 1,874,459 _1601 Pf6N2E2_1602 - hypothetical protein, at 1,874,530 to 1,875,312 _1602 Pf6N2E2_1603 - hypothetical protein, at 1,875,476 to 1,875,904 _1603 Pf6N2E2_1604 - Acetyltransferase, at 1,875,967 to 1,876,467 _1604 Position (kb) 1874 1875 1876Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1873.595 kb on + strand, within Pf6N2E2_1600at 1873.595 kb on + strand, within Pf6N2E2_1600at 1873.596 kb on - strand, within Pf6N2E2_1600at 1873.889 kb on + strandat 1873.890 kb on - strandat 1874.285 kb on + strand, within Pf6N2E2_1601at 1874.340 kb on + strand, within Pf6N2E2_1601at 1874.340 kb on + strand, within Pf6N2E2_1601at 1874.340 kb on + strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.343 kb on - strand, within Pf6N2E2_1601at 1874.391 kb on + strand, within Pf6N2E2_1601at 1874.391 kb on + strand, within Pf6N2E2_1601at 1874.392 kb on - strand, within Pf6N2E2_1601at 1874.508 kb on - strandat 1874.543 kb on + strandat 1874.543 kb on + strandat 1874.543 kb on + strandat 1874.766 kb on + strand, within Pf6N2E2_1602at 1874.766 kb on + strand, within Pf6N2E2_1602at 1874.768 kb on + strand, within Pf6N2E2_1602at 1874.768 kb on + strand, within Pf6N2E2_1602at 1874.831 kb on + strand, within Pf6N2E2_1602at 1874.936 kb on + strand, within Pf6N2E2_1602at 1875.005 kb on + strand, within Pf6N2E2_1602at 1875.154 kb on - strand, within Pf6N2E2_1602at 1875.402 kb on - strandat 1875.402 kb on - strandat 1875.402 kb on - strandat 1875.404 kb on - strandat 1875.418 kb on - strandat 1875.419 kb on + strandat 1875.444 kb on + strandat 1875.445 kb on - strandat 1875.499 kb on + strandat 1875.518 kb on - strandat 1875.521 kb on + strand, within Pf6N2E2_1603at 1875.521 kb on + strand, within Pf6N2E2_1603at 1875.522 kb on - strand, within Pf6N2E2_1603at 1875.522 kb on - strand, within Pf6N2E2_1603at 1875.626 kb on + strand, within Pf6N2E2_1603at 1875.822 kb on - strand, within Pf6N2E2_1603at 1875.929 kb on - strandat 1875.961 kb on - strandat 1875.969 kb on - strandat 1876.085 kb on + strand, within Pf6N2E2_1604at 1876.085 kb on + strand, within Pf6N2E2_1604at 1876.085 kb on + strand, within Pf6N2E2_1604at 1876.085 kb on + strand, within Pf6N2E2_1604at 1876.085 kb on + strand, within Pf6N2E2_1604at 1876.086 kb on - strand, within Pf6N2E2_1604at 1876.086 kb on - strand, within Pf6N2E2_1604at 1876.086 kb on - strand, within Pf6N2E2_1604at 1876.086 kb on - strand, within Pf6N2E2_1604

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM
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1,873,595 + Pf6N2E2_1600 0.83 +1.9
1,873,595 + Pf6N2E2_1600 0.83 +2.6
1,873,596 - Pf6N2E2_1600 0.83 -0.9
1,873,889 + +0.9
1,873,890 - +0.8
1,874,285 + Pf6N2E2_1601 0.70 -0.2
1,874,340 + Pf6N2E2_1601 0.79 +1.9
1,874,340 + Pf6N2E2_1601 0.79 +1.9
1,874,340 + Pf6N2E2_1601 0.79 -2.3
1,874,341 - Pf6N2E2_1601 0.80 +0.3
1,874,341 - Pf6N2E2_1601 0.80 +0.5
1,874,341 - Pf6N2E2_1601 0.80 -1.6
1,874,341 - Pf6N2E2_1601 0.80 +0.9
1,874,341 - Pf6N2E2_1601 0.80 -0.1
1,874,341 - Pf6N2E2_1601 0.80 -1.8
1,874,341 - Pf6N2E2_1601 0.80 +0.4
1,874,341 - Pf6N2E2_1601 0.80 -2.5
1,874,341 - Pf6N2E2_1601 0.80 -0.2
1,874,343 - Pf6N2E2_1601 0.80 -0.9
1,874,391 + Pf6N2E2_1601 0.88 +0.9
1,874,391 + Pf6N2E2_1601 0.88 -3.6
1,874,392 - Pf6N2E2_1601 0.88 -0.2
1,874,508 - -0.5
1,874,543 + -1.4
1,874,543 + -1.3
1,874,543 + +1.1
1,874,766 + Pf6N2E2_1602 0.30 +1.9
1,874,766 + Pf6N2E2_1602 0.30 -0.5
1,874,768 + Pf6N2E2_1602 0.30 -0.1
1,874,768 + Pf6N2E2_1602 0.30 -1.1
1,874,831 + Pf6N2E2_1602 0.38 +1.9
1,874,936 + Pf6N2E2_1602 0.52 +0.0
1,875,005 + Pf6N2E2_1602 0.61 -0.7
1,875,154 - Pf6N2E2_1602 0.80 +1.9
1,875,402 - -1.8
1,875,402 - -1.7
1,875,402 - +1.9
1,875,404 - -1.3
1,875,418 - -0.3
1,875,419 + -2.4
1,875,444 + +0.9
1,875,445 - -0.6
1,875,499 + +0.3
1,875,518 - -0.2
1,875,521 + Pf6N2E2_1603 0.10 -0.6
1,875,521 + Pf6N2E2_1603 0.10 +2.7
1,875,522 - Pf6N2E2_1603 0.11 -0.4
1,875,522 - Pf6N2E2_1603 0.11 -0.2
1,875,626 + Pf6N2E2_1603 0.35 +0.6
1,875,822 - Pf6N2E2_1603 0.81 +0.7
1,875,929 - -1.3
1,875,961 - +1.9
1,875,969 - +0.3
1,876,085 + Pf6N2E2_1604 0.24 -3.1
1,876,085 + Pf6N2E2_1604 0.24 -0.2
1,876,085 + Pf6N2E2_1604 0.24 -0.8
1,876,085 + Pf6N2E2_1604 0.24 -0.4
1,876,085 + Pf6N2E2_1604 0.24 -0.2
1,876,086 - Pf6N2E2_1604 0.24 -3.1
1,876,086 - Pf6N2E2_1604 0.24 +0.5
1,876,086 - Pf6N2E2_1604 0.24 +1.9
1,876,086 - Pf6N2E2_1604 0.24 -2.0

Or see this region's nucleotide sequence