Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1600 and Pf6N2E2_1601 are separated by 5 nucleotides Pf6N2E2_1601 and Pf6N2E2_1602 are separated by 70 nucleotides Pf6N2E2_1602 and Pf6N2E2_1603 are separated by 163 nucleotides Pf6N2E2_1603 and Pf6N2E2_1604 are separated by 62 nucleotides
Pf6N2E2_1600 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 1,872,220 to 1,873,872
_1600
Pf6N2E2_1601 - DNA-binding response regulator, LuxR family, at 1,873,878 to 1,874,459
_1601
Pf6N2E2_1602 - hypothetical protein, at 1,874,530 to 1,875,312
_1602
Pf6N2E2_1603 - hypothetical protein, at 1,875,476 to 1,875,904
_1603
Pf6N2E2_1604 - Acetyltransferase, at 1,875,967 to 1,876,467
_1604
Position (kb)
1874
1875
1876 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1873.595 kb on + strand, within Pf6N2E2_1600 at 1873.595 kb on + strand, within Pf6N2E2_1600 at 1873.596 kb on - strand, within Pf6N2E2_1600 at 1873.889 kb on + strand at 1873.890 kb on - strand at 1874.285 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.343 kb on - strand, within Pf6N2E2_1601 at 1874.391 kb on + strand, within Pf6N2E2_1601 at 1874.391 kb on + strand, within Pf6N2E2_1601 at 1874.392 kb on - strand, within Pf6N2E2_1601 at 1874.508 kb on - strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.831 kb on + strand, within Pf6N2E2_1602 at 1874.936 kb on + strand, within Pf6N2E2_1602 at 1875.005 kb on + strand, within Pf6N2E2_1602 at 1875.154 kb on - strand, within Pf6N2E2_1602 at 1875.402 kb on - strand at 1875.402 kb on - strand at 1875.402 kb on - strand at 1875.404 kb on - strand at 1875.418 kb on - strand at 1875.419 kb on + strand at 1875.444 kb on + strand at 1875.445 kb on - strand at 1875.499 kb on + strand at 1875.518 kb on - strand at 1875.521 kb on + strand, within Pf6N2E2_1603 at 1875.521 kb on + strand, within Pf6N2E2_1603 at 1875.522 kb on - strand, within Pf6N2E2_1603 at 1875.522 kb on - strand, within Pf6N2E2_1603 at 1875.626 kb on + strand, within Pf6N2E2_1603 at 1875.822 kb on - strand, within Pf6N2E2_1603 at 1875.929 kb on - strand at 1875.961 kb on - strand at 1875.969 kb on - strand at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 1,873,595 + Pf6N2E2_1600 0.83 +1.9 1,873,595 + Pf6N2E2_1600 0.83 +2.6 1,873,596 - Pf6N2E2_1600 0.83 -0.9 1,873,889 + +0.9 1,873,890 - +0.8 1,874,285 + Pf6N2E2_1601 0.70 -0.2 1,874,340 + Pf6N2E2_1601 0.79 +1.9 1,874,340 + Pf6N2E2_1601 0.79 +1.9 1,874,340 + Pf6N2E2_1601 0.79 -2.3 1,874,341 - Pf6N2E2_1601 0.80 +0.3 1,874,341 - Pf6N2E2_1601 0.80 +0.5 1,874,341 - Pf6N2E2_1601 0.80 -1.6 1,874,341 - Pf6N2E2_1601 0.80 +0.9 1,874,341 - Pf6N2E2_1601 0.80 -0.1 1,874,341 - Pf6N2E2_1601 0.80 -1.8 1,874,341 - Pf6N2E2_1601 0.80 +0.4 1,874,341 - Pf6N2E2_1601 0.80 -2.5 1,874,341 - Pf6N2E2_1601 0.80 -0.2 1,874,343 - Pf6N2E2_1601 0.80 -0.9 1,874,391 + Pf6N2E2_1601 0.88 +0.9 1,874,391 + Pf6N2E2_1601 0.88 -3.6 1,874,392 - Pf6N2E2_1601 0.88 -0.2 1,874,508 - -0.5 1,874,543 + -1.4 1,874,543 + -1.3 1,874,543 + +1.1 1,874,766 + Pf6N2E2_1602 0.30 +1.9 1,874,766 + Pf6N2E2_1602 0.30 -0.5 1,874,768 + Pf6N2E2_1602 0.30 -0.1 1,874,768 + Pf6N2E2_1602 0.30 -1.1 1,874,831 + Pf6N2E2_1602 0.38 +1.9 1,874,936 + Pf6N2E2_1602 0.52 +0.0 1,875,005 + Pf6N2E2_1602 0.61 -0.7 1,875,154 - Pf6N2E2_1602 0.80 +1.9 1,875,402 - -1.8 1,875,402 - -1.7 1,875,402 - +1.9 1,875,404 - -1.3 1,875,418 - -0.3 1,875,419 + -2.4 1,875,444 + +0.9 1,875,445 - -0.6 1,875,499 + +0.3 1,875,518 - -0.2 1,875,521 + Pf6N2E2_1603 0.10 -0.6 1,875,521 + Pf6N2E2_1603 0.10 +2.7 1,875,522 - Pf6N2E2_1603 0.11 -0.4 1,875,522 - Pf6N2E2_1603 0.11 -0.2 1,875,626 + Pf6N2E2_1603 0.35 +0.6 1,875,822 - Pf6N2E2_1603 0.81 +0.7 1,875,929 - -1.3 1,875,961 - +1.9 1,875,969 - +0.3 1,876,085 + Pf6N2E2_1604 0.24 -3.1 1,876,085 + Pf6N2E2_1604 0.24 -0.2 1,876,085 + Pf6N2E2_1604 0.24 -0.8 1,876,085 + Pf6N2E2_1604 0.24 -0.4 1,876,085 + Pf6N2E2_1604 0.24 -0.2 1,876,086 - Pf6N2E2_1604 0.24 -3.1 1,876,086 - Pf6N2E2_1604 0.24 +0.5 1,876,086 - Pf6N2E2_1604 0.24 +1.9 1,876,086 - Pf6N2E2_1604 0.24 -2.0
Or see this region's nucleotide sequence