Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_90 and Pf6N2E2_91 are separated by 141 nucleotides Pf6N2E2_91 and Pf6N2E2_92 are separated by 106 nucleotides
Pf6N2E2_90 - Lipoprotein, putative, at 135,485 to 136,639
_90
Pf6N2E2_91 - Transcriptional regulator, LysR family, at 136,781 to 137,665
_91
Pf6N2E2_92 - hypothetical protein, at 137,772 to 138,221
_92
Position (kb) 136
137
138 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 135.940 kb on - strand, within Pf6N2E2_90 at 136.015 kb on + strand, within Pf6N2E2_90 at 136.264 kb on + strand, within Pf6N2E2_90 at 136.352 kb on + strand, within Pf6N2E2_90 at 136.352 kb on + strand, within Pf6N2E2_90 at 136.369 kb on + strand, within Pf6N2E2_90 at 136.397 kb on + strand, within Pf6N2E2_90 at 136.547 kb on - strand at 136.567 kb on + strand at 136.568 kb on - strand at 136.613 kb on + strand at 136.613 kb on + strand at 136.679 kb on + strand at 136.679 kb on + strand at 136.679 kb on + strand at 136.679 kb on + strand at 136.680 kb on - strand at 136.680 kb on - strand at 136.865 kb on - strand at 136.865 kb on - strand at 136.866 kb on + strand at 137.163 kb on + strand, within Pf6N2E2_91 at 137.164 kb on - strand, within Pf6N2E2_91 at 137.285 kb on - strand, within Pf6N2E2_91 at 137.448 kb on + strand, within Pf6N2E2_91 at 137.448 kb on + strand, within Pf6N2E2_91 at 137.449 kb on - strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.679 kb on - strand at 137.703 kb on + strand at 137.780 kb on + strand at 137.780 kb on + strand at 137.861 kb on - strand, within Pf6N2E2_92 at 138.001 kb on + strand, within Pf6N2E2_92 at 138.002 kb on - strand, within Pf6N2E2_92 at 138.115 kb on + strand, within Pf6N2E2_92 at 138.116 kb on - strand, within Pf6N2E2_92 at 138.325 kb on + strand at 138.575 kb on + strand at 138.576 kb on - strand at 138.576 kb on - strand at 138.614 kb on + strand at 138.614 kb on + strand at 138.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 135,940 - Pf6N2E2_90 0.39 -1.9 136,015 + Pf6N2E2_90 0.46 +1.0 136,264 + Pf6N2E2_90 0.67 +0.2 136,352 + Pf6N2E2_90 0.75 +0.6 136,352 + Pf6N2E2_90 0.75 +1.4 136,369 + Pf6N2E2_90 0.77 -0.0 136,397 + Pf6N2E2_90 0.79 +2.0 136,547 - -0.6 136,567 + +0.9 136,568 - +0.3 136,613 + -2.5 136,613 + -3.8 136,679 + +0.2 136,679 + +0.3 136,679 + -0.0 136,679 + +1.0 136,680 - -0.8 136,680 - +0.5 136,865 - -0.2 136,865 - -0.8 136,866 + -2.4 137,163 + Pf6N2E2_91 0.43 -1.6 137,164 - Pf6N2E2_91 0.43 +0.0 137,285 - Pf6N2E2_91 0.57 -0.8 137,448 + Pf6N2E2_91 0.75 -2.1 137,448 + Pf6N2E2_91 0.75 -0.7 137,449 - Pf6N2E2_91 0.75 +2.0 137,454 + Pf6N2E2_91 0.76 +0.8 137,454 + Pf6N2E2_91 0.76 -0.1 137,454 + Pf6N2E2_91 0.76 -0.3 137,454 + Pf6N2E2_91 0.76 +0.3 137,454 + Pf6N2E2_91 0.76 +0.1 137,454 + Pf6N2E2_91 0.76 -0.5 137,454 + Pf6N2E2_91 0.76 -0.7 137,454 + Pf6N2E2_91 0.76 -1.7 137,454 + Pf6N2E2_91 0.76 -0.1 137,455 - Pf6N2E2_91 0.76 +2.0 137,455 - Pf6N2E2_91 0.76 -2.1 137,455 - Pf6N2E2_91 0.76 +0.6 137,455 - Pf6N2E2_91 0.76 -3.0 137,455 - Pf6N2E2_91 0.76 -0.7 137,455 - Pf6N2E2_91 0.76 -0.0 137,455 - Pf6N2E2_91 0.76 -0.4 137,455 - Pf6N2E2_91 0.76 +1.0 137,455 - Pf6N2E2_91 0.76 -1.4 137,679 - -0.8 137,703 + -0.3 137,780 + +2.0 137,780 + +0.0 137,861 - Pf6N2E2_92 0.20 -2.4 138,001 + Pf6N2E2_92 0.51 -0.4 138,002 - Pf6N2E2_92 0.51 -2.0 138,115 + Pf6N2E2_92 0.76 +0.7 138,116 - Pf6N2E2_92 0.76 +1.4 138,325 + -0.8 138,575 + -1.5 138,576 - -1.0 138,576 - -0.0 138,614 + -0.4 138,614 + -0.2 138,615 - +0.4
Or see this region's nucleotide sequence