Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_854 and Pf6N2E2_855 are separated by 174 nucleotides Pf6N2E2_855 and Pf6N2E2_856 are separated by 45 nucleotides
Pf6N2E2_854 - Xylose isomerase domain protein TIM barrel, at 1,000,173 to 1,000,982
_854
Pf6N2E2_855 - Transcriptional regulator, IclR family, at 1,001,157 to 1,001,963
_855
Pf6N2E2_856 - Oxidoreductase, putative, at 1,002,009 to 1,003,016
_856
Position (kb)
1001
1002 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1000.195 kb on - strand at 1000.344 kb on + strand, within Pf6N2E2_854 at 1000.412 kb on + strand, within Pf6N2E2_854 at 1000.472 kb on + strand, within Pf6N2E2_854 at 1000.472 kb on + strand, within Pf6N2E2_854 at 1000.540 kb on - strand, within Pf6N2E2_854 at 1000.551 kb on + strand, within Pf6N2E2_854 at 1000.551 kb on + strand, within Pf6N2E2_854 at 1000.603 kb on + strand, within Pf6N2E2_854 at 1000.604 kb on - strand, within Pf6N2E2_854 at 1000.624 kb on - strand, within Pf6N2E2_854 at 1000.663 kb on + strand, within Pf6N2E2_854 at 1000.663 kb on + strand, within Pf6N2E2_854 at 1000.663 kb on + strand, within Pf6N2E2_854 at 1000.663 kb on + strand, within Pf6N2E2_854 at 1000.663 kb on + strand, within Pf6N2E2_854 at 1000.663 kb on + strand, within Pf6N2E2_854 at 1000.664 kb on - strand, within Pf6N2E2_854 at 1000.664 kb on - strand, within Pf6N2E2_854 at 1000.719 kb on + strand, within Pf6N2E2_854 at 1000.719 kb on + strand, within Pf6N2E2_854 at 1000.719 kb on + strand, within Pf6N2E2_854 at 1000.719 kb on + strand, within Pf6N2E2_854 at 1000.719 kb on + strand, within Pf6N2E2_854 at 1000.719 kb on + strand, within Pf6N2E2_854 at 1000.720 kb on - strand, within Pf6N2E2_854 at 1000.720 kb on - strand, within Pf6N2E2_854 at 1000.755 kb on + strand, within Pf6N2E2_854 at 1000.755 kb on + strand, within Pf6N2E2_854 at 1000.755 kb on + strand, within Pf6N2E2_854 at 1000.755 kb on + strand, within Pf6N2E2_854 at 1000.804 kb on + strand, within Pf6N2E2_854 at 1000.804 kb on + strand, within Pf6N2E2_854 at 1000.804 kb on + strand, within Pf6N2E2_854 at 1000.805 kb on - strand, within Pf6N2E2_854 at 1000.805 kb on - strand, within Pf6N2E2_854 at 1000.835 kb on - strand, within Pf6N2E2_854 at 1000.835 kb on - strand, within Pf6N2E2_854 at 1000.895 kb on - strand, within Pf6N2E2_854 at 1000.926 kb on + strand at 1000.926 kb on + strand at 1000.926 kb on + strand at 1001.069 kb on + strand at 1001.218 kb on - strand at 1001.347 kb on - strand, within Pf6N2E2_855 at 1001.548 kb on - strand, within Pf6N2E2_855 at 1001.548 kb on - strand, within Pf6N2E2_855 at 1001.819 kb on - strand, within Pf6N2E2_855 at 1002.151 kb on - strand, within Pf6N2E2_856 at 1002.164 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.167 kb on - strand, within Pf6N2E2_856 at 1002.254 kb on - strand, within Pf6N2E2_856 at 1002.254 kb on - strand, within Pf6N2E2_856 at 1002.254 kb on - strand, within Pf6N2E2_856 at 1002.254 kb on - strand, within Pf6N2E2_856 at 1002.284 kb on - strand, within Pf6N2E2_856 at 1002.433 kb on - strand, within Pf6N2E2_856 at 1002.547 kb on + strand, within Pf6N2E2_856 at 1002.636 kb on + strand, within Pf6N2E2_856 at 1002.637 kb on - strand, within Pf6N2E2_856 at 1002.712 kb on - strand, within Pf6N2E2_856 at 1002.712 kb on - strand, within Pf6N2E2_856 at 1002.865 kb on + strand, within Pf6N2E2_856 at 1002.865 kb on + strand, within Pf6N2E2_856 at 1002.866 kb on - strand, within Pf6N2E2_856
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 1,000,195 - -2.3 1,000,344 + Pf6N2E2_854 0.21 +0.4 1,000,412 + Pf6N2E2_854 0.30 +1.9 1,000,472 + Pf6N2E2_854 0.37 -0.1 1,000,472 + Pf6N2E2_854 0.37 -3.3 1,000,540 - Pf6N2E2_854 0.45 -2.1 1,000,551 + Pf6N2E2_854 0.47 -0.6 1,000,551 + Pf6N2E2_854 0.47 +1.0 1,000,603 + Pf6N2E2_854 0.53 +0.0 1,000,604 - Pf6N2E2_854 0.53 +0.1 1,000,624 - Pf6N2E2_854 0.56 +0.6 1,000,663 + Pf6N2E2_854 0.60 -0.5 1,000,663 + Pf6N2E2_854 0.60 +0.3 1,000,663 + Pf6N2E2_854 0.60 +0.9 1,000,663 + Pf6N2E2_854 0.60 -2.1 1,000,663 + Pf6N2E2_854 0.60 +0.4 1,000,663 + Pf6N2E2_854 0.60 +0.2 1,000,664 - Pf6N2E2_854 0.61 +0.7 1,000,664 - Pf6N2E2_854 0.61 +0.9 1,000,719 + Pf6N2E2_854 0.67 +0.9 1,000,719 + Pf6N2E2_854 0.67 +0.4 1,000,719 + Pf6N2E2_854 0.67 +0.6 1,000,719 + Pf6N2E2_854 0.67 -1.3 1,000,719 + Pf6N2E2_854 0.67 +0.6 1,000,719 + Pf6N2E2_854 0.67 +0.4 1,000,720 - Pf6N2E2_854 0.68 -0.6 1,000,720 - Pf6N2E2_854 0.68 +0.6 1,000,755 + Pf6N2E2_854 0.72 -0.2 1,000,755 + Pf6N2E2_854 0.72 +1.8 1,000,755 + Pf6N2E2_854 0.72 +0.5 1,000,755 + Pf6N2E2_854 0.72 -2.0 1,000,804 + Pf6N2E2_854 0.78 +1.4 1,000,804 + Pf6N2E2_854 0.78 -0.0 1,000,804 + Pf6N2E2_854 0.78 +0.4 1,000,805 - Pf6N2E2_854 0.78 +0.6 1,000,805 - Pf6N2E2_854 0.78 -1.6 1,000,835 - Pf6N2E2_854 0.82 +1.9 1,000,835 - Pf6N2E2_854 0.82 -0.1 1,000,895 - Pf6N2E2_854 0.89 -1.5 1,000,926 + +0.1 1,000,926 + +1.9 1,000,926 + -0.4 1,001,069 + -1.4 1,001,218 - +0.4 1,001,347 - Pf6N2E2_855 0.24 -0.9 1,001,548 - Pf6N2E2_855 0.48 +1.9 1,001,548 - Pf6N2E2_855 0.48 +1.9 1,001,819 - Pf6N2E2_855 0.82 -1.2 1,002,151 - Pf6N2E2_856 0.14 +1.3 1,002,164 - Pf6N2E2_856 0.15 -2.8 1,002,167 - Pf6N2E2_856 0.16 -0.8 1,002,167 - Pf6N2E2_856 0.16 -0.1 1,002,167 - Pf6N2E2_856 0.16 -2.7 1,002,167 - Pf6N2E2_856 0.16 +0.6 1,002,167 - Pf6N2E2_856 0.16 +0.9 1,002,167 - Pf6N2E2_856 0.16 -1.2 1,002,167 - Pf6N2E2_856 0.16 +1.9 1,002,167 - Pf6N2E2_856 0.16 -0.4 1,002,167 - Pf6N2E2_856 0.16 -1.7 1,002,254 - Pf6N2E2_856 0.24 +1.9 1,002,254 - Pf6N2E2_856 0.24 -0.3 1,002,254 - Pf6N2E2_856 0.24 -1.1 1,002,254 - Pf6N2E2_856 0.24 -0.6 1,002,284 - Pf6N2E2_856 0.27 +0.9 1,002,433 - Pf6N2E2_856 0.42 -2.0 1,002,547 + Pf6N2E2_856 0.53 -1.8 1,002,636 + Pf6N2E2_856 0.62 -1.6 1,002,637 - Pf6N2E2_856 0.62 +1.9 1,002,712 - Pf6N2E2_856 0.70 +1.9 1,002,712 - Pf6N2E2_856 0.70 +1.9 1,002,865 + Pf6N2E2_856 0.85 +0.3 1,002,865 + Pf6N2E2_856 0.85 -0.3 1,002,866 - Pf6N2E2_856 0.85 +0.1
Or see this region's nucleotide sequence