Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_609 and Pf6N2E2_610 are separated by 193 nucleotides Pf6N2E2_610 and Pf6N2E2_611 are separated by 219 nucleotides
Pf6N2E2_609 - L-arabonate dehydratase (EC 4.2.1.25) (from data) , at 691,355 to 693,091
_609
Pf6N2E2_610 - Nitrate/nitrite transporter, at 693,285 to 694,607
_610
Pf6N2E2_611 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data) , at 694,827 to 695,819
_611
Position (kb)
693
694
695 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 692.386 kb on + strand, within Pf6N2E2_609 at 692.386 kb on + strand, within Pf6N2E2_609 at 692.387 kb on - strand, within Pf6N2E2_609 at 692.400 kb on + strand, within Pf6N2E2_609 at 693.306 kb on - strand at 693.317 kb on + strand at 693.468 kb on + strand, within Pf6N2E2_610 at 693.468 kb on + strand, within Pf6N2E2_610 at 693.468 kb on + strand, within Pf6N2E2_610 at 693.468 kb on + strand, within Pf6N2E2_610 at 693.469 kb on - strand, within Pf6N2E2_610 at 693.580 kb on + strand, within Pf6N2E2_610 at 693.581 kb on - strand, within Pf6N2E2_610 at 693.593 kb on + strand, within Pf6N2E2_610 at 693.600 kb on + strand, within Pf6N2E2_610 at 693.600 kb on + strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.601 kb on - strand, within Pf6N2E2_610 at 693.657 kb on + strand, within Pf6N2E2_610 at 693.657 kb on + strand, within Pf6N2E2_610 at 693.657 kb on + strand, within Pf6N2E2_610 at 693.658 kb on - strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.660 kb on + strand, within Pf6N2E2_610 at 693.661 kb on - strand, within Pf6N2E2_610 at 693.728 kb on + strand, within Pf6N2E2_610 at 693.728 kb on + strand, within Pf6N2E2_610 at 693.728 kb on + strand, within Pf6N2E2_610 at 693.729 kb on - strand, within Pf6N2E2_610 at 693.729 kb on - strand, within Pf6N2E2_610 at 693.865 kb on - strand, within Pf6N2E2_610 at 694.027 kb on - strand, within Pf6N2E2_610 at 694.134 kb on + strand, within Pf6N2E2_610 at 694.134 kb on + strand, within Pf6N2E2_610 at 694.134 kb on + strand, within Pf6N2E2_610 at 694.135 kb on - strand, within Pf6N2E2_610 at 694.218 kb on + strand, within Pf6N2E2_610 at 694.218 kb on + strand, within Pf6N2E2_610 at 694.218 kb on + strand, within Pf6N2E2_610 at 694.219 kb on - strand, within Pf6N2E2_610 at 694.219 kb on - strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.222 kb on + strand, within Pf6N2E2_610 at 694.223 kb on - strand, within Pf6N2E2_610 at 694.416 kb on + strand, within Pf6N2E2_610 at 694.452 kb on + strand, within Pf6N2E2_610 at 694.479 kb on + strand at 694.480 kb on - strand at 694.601 kb on + strand at 694.796 kb on + strand at 694.796 kb on + strand at 694.797 kb on - strand at 694.834 kb on + strand at 694.835 kb on - strand at 694.835 kb on - strand at 695.007 kb on + strand, within Pf6N2E2_611 at 695.245 kb on - strand, within Pf6N2E2_611 at 695.343 kb on + strand, within Pf6N2E2_611 at 695.343 kb on + strand at 695.343 kb on + strand, within Pf6N2E2_611 at 695.343 kb on + strand, within Pf6N2E2_611 at 695.344 kb on - strand, within Pf6N2E2_611 at 695.344 kb on - strand, within Pf6N2E2_611 at 695.430 kb on + strand, within Pf6N2E2_611 at 695.430 kb on + strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.436 kb on + strand, within Pf6N2E2_611
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 692,386 + Pf6N2E2_609 0.59 -0.9 692,386 + Pf6N2E2_609 0.59 +0.9 692,387 - Pf6N2E2_609 0.59 +0.3 692,400 + Pf6N2E2_609 0.60 +0.0 693,306 - +0.8 693,317 + -1.4 693,468 + Pf6N2E2_610 0.14 +0.5 693,468 + Pf6N2E2_610 0.14 +1.9 693,468 + Pf6N2E2_610 0.14 +1.9 693,468 + Pf6N2E2_610 0.14 +0.2 693,469 - Pf6N2E2_610 0.14 +1.8 693,580 + Pf6N2E2_610 0.22 -1.1 693,581 - Pf6N2E2_610 0.22 -2.5 693,593 + Pf6N2E2_610 0.23 -1.0 693,600 + Pf6N2E2_610 0.24 +0.9 693,600 + Pf6N2E2_610 0.24 -1.6 693,601 - Pf6N2E2_610 0.24 -2.4 693,601 - Pf6N2E2_610 0.24 -2.3 693,601 - Pf6N2E2_610 0.24 -3.3 693,601 - Pf6N2E2_610 0.24 +0.1 693,657 + Pf6N2E2_610 0.28 -1.1 693,657 + Pf6N2E2_610 0.28 +0.2 693,657 + Pf6N2E2_610 0.28 +0.9 693,658 - Pf6N2E2_610 0.28 -0.3 693,660 + Pf6N2E2_610 0.28 +1.3 693,660 + Pf6N2E2_610 0.28 -0.2 693,660 + Pf6N2E2_610 0.28 +0.9 693,660 + Pf6N2E2_610 0.28 +3.4 693,660 + Pf6N2E2_610 0.28 -0.0 693,661 - Pf6N2E2_610 0.28 +1.3 693,728 + Pf6N2E2_610 0.33 +0.9 693,728 + Pf6N2E2_610 0.33 -0.1 693,728 + Pf6N2E2_610 0.33 +0.1 693,729 - Pf6N2E2_610 0.34 -2.4 693,729 - Pf6N2E2_610 0.34 +1.9 693,865 - Pf6N2E2_610 0.44 +0.4 694,027 - Pf6N2E2_610 0.56 -1.7 694,134 + Pf6N2E2_610 0.64 +0.9 694,134 + Pf6N2E2_610 0.64 +0.2 694,134 + Pf6N2E2_610 0.64 -0.5 694,135 - Pf6N2E2_610 0.64 +0.9 694,218 + Pf6N2E2_610 0.71 +0.3 694,218 + Pf6N2E2_610 0.71 -0.5 694,218 + Pf6N2E2_610 0.71 -0.6 694,219 - Pf6N2E2_610 0.71 +0.9 694,219 - Pf6N2E2_610 0.71 +1.8 694,222 + Pf6N2E2_610 0.71 +1.1 694,222 + Pf6N2E2_610 0.71 +1.9 694,222 + Pf6N2E2_610 0.71 -0.3 694,222 + Pf6N2E2_610 0.71 +0.9 694,222 + Pf6N2E2_610 0.71 +0.2 694,223 - Pf6N2E2_610 0.71 -0.2 694,416 + Pf6N2E2_610 0.85 +1.2 694,452 + Pf6N2E2_610 0.88 -0.9 694,479 + +1.9 694,480 - +0.7 694,601 + -0.7 694,796 + +1.7 694,796 + +0.9 694,797 - -0.1 694,834 + -0.3 694,835 - +0.1 694,835 - +0.6 695,007 + Pf6N2E2_611 0.18 -0.4 695,245 - Pf6N2E2_611 0.42 +1.9 695,343 + Pf6N2E2_611 0.52 +1.1 695,343 + -0.4 695,343 + Pf6N2E2_611 0.52 -2.8 695,343 + Pf6N2E2_611 0.52 -2.7 695,344 - Pf6N2E2_611 0.52 +0.9 695,344 - Pf6N2E2_611 0.52 +0.1 695,430 + Pf6N2E2_611 0.61 +0.9 695,430 + Pf6N2E2_611 0.61 -2.5 695,431 - Pf6N2E2_611 0.61 -0.3 695,431 - Pf6N2E2_611 0.61 +0.2 695,431 - Pf6N2E2_611 0.61 -2.1 695,436 + Pf6N2E2_611 0.61 -2.4
Or see this region's nucleotide sequence