Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5258 and Pf6N2E2_5259 are separated by 132 nucleotides Pf6N2E2_5259 and Pf6N2E2_5260 are separated by 0 nucleotides
Pf6N2E2_5258 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25), at 6,052,093 to 6,053,991
_5258
Pf6N2E2_5259 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 6,054,124 to 6,055,032
_5259
Pf6N2E2_5260 - Phosphate acetyltransferase (EC 2.3.1.8), at 6,055,033 to 6,057,132
_5260
Position (kb)
6054
6055
6056 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6053.149 kb on + strand, within Pf6N2E2_5258 at 6053.149 kb on + strand, within Pf6N2E2_5258 at 6053.149 kb on + strand, within Pf6N2E2_5258 at 6053.173 kb on + strand, within Pf6N2E2_5258 at 6053.174 kb on - strand, within Pf6N2E2_5258 at 6053.174 kb on - strand, within Pf6N2E2_5258 at 6053.647 kb on - strand, within Pf6N2E2_5258 at 6053.791 kb on + strand, within Pf6N2E2_5258 at 6053.791 kb on + strand, within Pf6N2E2_5258 at 6053.792 kb on - strand, within Pf6N2E2_5258 at 6053.842 kb on + strand at 6053.842 kb on + strand at 6053.843 kb on - strand at 6053.843 kb on - strand at 6053.843 kb on - strand at 6053.843 kb on - strand at 6053.884 kb on + strand at 6053.885 kb on - strand at 6053.988 kb on - strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.989 kb on + strand at 6053.990 kb on - strand at 6053.990 kb on - strand at 6053.990 kb on - strand at 6053.990 kb on - strand at 6053.990 kb on - strand at 6053.990 kb on - strand at 6054.006 kb on - strand at 6054.030 kb on + strand at 6054.030 kb on + strand at 6054.031 kb on - strand at 6054.031 kb on - strand at 6054.031 kb on - strand at 6054.114 kb on + strand at 6054.114 kb on + strand at 6054.132 kb on + strand at 6054.132 kb on + strand at 6054.132 kb on + strand at 6054.141 kb on - strand at 6054.236 kb on + strand, within Pf6N2E2_5259 at 6054.237 kb on - strand, within Pf6N2E2_5259 at 6054.237 kb on - strand, within Pf6N2E2_5259 at 6054.590 kb on + strand, within Pf6N2E2_5259 at 6054.590 kb on + strand, within Pf6N2E2_5259 at 6054.590 kb on + strand, within Pf6N2E2_5259 at 6054.590 kb on + strand, within Pf6N2E2_5259 at 6054.602 kb on + strand, within Pf6N2E2_5259 at 6054.602 kb on + strand, within Pf6N2E2_5259 at 6054.716 kb on + strand, within Pf6N2E2_5259 at 6054.716 kb on + strand, within Pf6N2E2_5259 at 6054.717 kb on - strand, within Pf6N2E2_5259 at 6054.717 kb on - strand, within Pf6N2E2_5259 at 6054.717 kb on - strand, within Pf6N2E2_5259 at 6054.768 kb on - strand, within Pf6N2E2_5259 at 6055.034 kb on + strand at 6055.034 kb on + strand at 6055.034 kb on + strand at 6055.034 kb on + strand at 6055.034 kb on + strand at 6055.035 kb on - strand at 6055.035 kb on - strand at 6055.035 kb on - strand at 6055.035 kb on - strand at 6055.035 kb on - strand at 6055.085 kb on + strand at 6055.086 kb on - strand at 6055.086 kb on - strand at 6055.086 kb on - strand at 6055.086 kb on - strand at 6055.086 kb on - strand at 6055.415 kb on + strand, within Pf6N2E2_5260 at 6055.415 kb on + strand, within Pf6N2E2_5260 at 6055.415 kb on + strand, within Pf6N2E2_5260 at 6055.416 kb on - strand, within Pf6N2E2_5260 at 6055.416 kb on - strand, within Pf6N2E2_5260 at 6055.532 kb on + strand, within Pf6N2E2_5260 at 6055.784 kb on + strand, within Pf6N2E2_5260 at 6055.785 kb on - strand, within Pf6N2E2_5260 at 6055.968 kb on + strand, within Pf6N2E2_5260 at 6055.969 kb on - strand, within Pf6N2E2_5260
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 6,053,149 + Pf6N2E2_5258 0.56 -0.3 6,053,149 + Pf6N2E2_5258 0.56 -1.5 6,053,149 + Pf6N2E2_5258 0.56 -3.1 6,053,173 + Pf6N2E2_5258 0.57 -2.7 6,053,174 - Pf6N2E2_5258 0.57 -1.9 6,053,174 - Pf6N2E2_5258 0.57 -1.3 6,053,647 - Pf6N2E2_5258 0.82 +0.5 6,053,791 + Pf6N2E2_5258 0.89 +0.0 6,053,791 + Pf6N2E2_5258 0.89 -0.8 6,053,792 - Pf6N2E2_5258 0.89 +1.6 6,053,842 + -1.9 6,053,842 + -0.2 6,053,843 - +1.0 6,053,843 - +2.0 6,053,843 - -2.4 6,053,843 - -2.2 6,053,884 + -0.5 6,053,885 - +1.0 6,053,988 - +1.4 6,053,989 + -0.8 6,053,989 + -0.8 6,053,989 + +0.2 6,053,989 + +0.9 6,053,989 + -0.4 6,053,989 + -0.1 6,053,989 + -1.0 6,053,989 + +1.5 6,053,989 + +0.7 6,053,989 + -0.7 6,053,989 + -1.7 6,053,989 + -0.7 6,053,990 - +2.0 6,053,990 - -0.2 6,053,990 - -0.3 6,053,990 - +0.1 6,053,990 - -2.6 6,053,990 - +2.3 6,054,006 - +0.2 6,054,030 + +0.6 6,054,030 + +1.6 6,054,031 - +0.1 6,054,031 - +0.5 6,054,031 - -0.3 6,054,114 + +0.5 6,054,114 + +0.5 6,054,132 + +2.0 6,054,132 + -1.1 6,054,132 + -0.5 6,054,141 - +0.2 6,054,236 + Pf6N2E2_5259 0.12 -2.1 6,054,237 - Pf6N2E2_5259 0.12 -0.3 6,054,237 - Pf6N2E2_5259 0.12 +1.7 6,054,590 + Pf6N2E2_5259 0.51 -0.5 6,054,590 + Pf6N2E2_5259 0.51 -0.4 6,054,590 + Pf6N2E2_5259 0.51 -3.0 6,054,590 + Pf6N2E2_5259 0.51 +0.6 6,054,602 + Pf6N2E2_5259 0.53 -0.5 6,054,602 + Pf6N2E2_5259 0.53 -0.2 6,054,716 + Pf6N2E2_5259 0.65 -3.0 6,054,716 + Pf6N2E2_5259 0.65 -3.8 6,054,717 - Pf6N2E2_5259 0.65 -2.3 6,054,717 - Pf6N2E2_5259 0.65 +0.2 6,054,717 - Pf6N2E2_5259 0.65 -2.5 6,054,768 - Pf6N2E2_5259 0.71 -1.0 6,055,034 + +0.0 6,055,034 + +1.0 6,055,034 + +0.5 6,055,034 + -3.2 6,055,034 + -0.5 6,055,035 - -1.1 6,055,035 - +1.0 6,055,035 - -1.4 6,055,035 - +0.4 6,055,035 - -1.4 6,055,085 + -0.1 6,055,086 - +0.6 6,055,086 - +0.1 6,055,086 - +2.9 6,055,086 - +2.0 6,055,086 - -1.0 6,055,415 + Pf6N2E2_5260 0.18 -0.3 6,055,415 + Pf6N2E2_5260 0.18 +0.3 6,055,415 + Pf6N2E2_5260 0.18 +0.3 6,055,416 - Pf6N2E2_5260 0.18 -1.1 6,055,416 - Pf6N2E2_5260 0.18 -3.4 6,055,532 + Pf6N2E2_5260 0.24 -0.8 6,055,784 + Pf6N2E2_5260 0.36 -0.1 6,055,785 - Pf6N2E2_5260 0.36 -0.1 6,055,968 + Pf6N2E2_5260 0.45 -0.2 6,055,969 - Pf6N2E2_5260 0.45 -0.2
Or see this region's nucleotide sequence