Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_471 and Pf6N2E2_472 are separated by 288 nucleotides Pf6N2E2_472 and Pf6N2E2_473 are separated by 13 nucleotides Pf6N2E2_473 and Pf6N2E2_474 are separated by 82 nucleotides Pf6N2E2_474 and Pf6N2E2_475 are separated by 76 nucleotides
Pf6N2E2_471 - putative metalloprotease, at 536,431 to 537,492
_471
Pf6N2E2_472 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 537,781 to 538,122
_472
Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272
_473
Pf6N2E2_474 - Transcriptional regulator, ArsR family, at 539,355 to 540,110
_474
Pf6N2E2_475 - Transcriptional regulator, AraC family, at 540,187 to 540,981
_475
Position (kb) 538
539
540 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.452 kb on + strand at 537.452 kb on + strand at 537.452 kb on + strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.505 kb on - strand at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 538.031 kb on - strand, within Pf6N2E2_472 at 538.212 kb on - strand at 538.617 kb on + strand, within Pf6N2E2_473 at 538.617 kb on + strand, within Pf6N2E2_473 at 538.617 kb on + strand, within Pf6N2E2_473 at 538.617 kb on + strand, within Pf6N2E2_473 at 538.618 kb on - strand, within Pf6N2E2_473 at 538.655 kb on - strand, within Pf6N2E2_473 at 538.655 kb on - strand, within Pf6N2E2_473 at 538.736 kb on + strand, within Pf6N2E2_473 at 539.227 kb on - strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.310 kb on - strand at 539.310 kb on - strand at 539.312 kb on + strand at 539.577 kb on + strand, within Pf6N2E2_474 at 539.577 kb on + strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.697 kb on + strand, within Pf6N2E2_474 at 539.705 kb on + strand, within Pf6N2E2_474 at 539.706 kb on - strand, within Pf6N2E2_474 at 540.198 kb on + strand at 540.198 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 537,170 + Pf6N2E2_471 0.70 +0.3 537,170 + Pf6N2E2_471 0.70 +0.2 537,170 + Pf6N2E2_471 0.70 -0.4 537,170 + Pf6N2E2_471 0.70 -1.1 537,171 - Pf6N2E2_471 0.70 +0.5 537,171 - Pf6N2E2_471 0.70 -0.7 537,452 + -0.5 537,452 + -1.8 537,452 + -1.9 537,453 - +1.9 537,453 - +1.9 537,453 - +0.3 537,453 - -0.4 537,505 - +0.9 537,833 + Pf6N2E2_472 0.15 +0.2 537,833 + Pf6N2E2_472 0.15 -1.0 537,833 + Pf6N2E2_472 0.15 +0.1 537,904 + Pf6N2E2_472 0.36 +0.2 537,904 + Pf6N2E2_472 0.36 +0.3 537,905 - Pf6N2E2_472 0.36 +1.9 537,905 - Pf6N2E2_472 0.36 -0.4 538,031 - Pf6N2E2_472 0.73 -0.5 538,212 - -1.3 538,617 + Pf6N2E2_473 0.42 -2.8 538,617 + Pf6N2E2_473 0.42 -1.4 538,617 + Pf6N2E2_473 0.42 +0.3 538,617 + Pf6N2E2_473 0.42 -1.6 538,618 - Pf6N2E2_473 0.42 -0.7 538,655 - Pf6N2E2_473 0.46 -0.1 538,655 - Pf6N2E2_473 0.46 -0.3 538,736 + Pf6N2E2_473 0.53 -0.1 539,227 - +0.3 539,309 + +2.3 539,309 + -0.4 539,309 + -0.7 539,309 + +1.9 539,309 + -0.3 539,309 + +0.4 539,309 + +1.4 539,310 - +0.9 539,310 - -0.7 539,312 + +0.3 539,577 + Pf6N2E2_474 0.29 -0.8 539,577 + Pf6N2E2_474 0.29 -1.9 539,578 - Pf6N2E2_474 0.29 -0.2 539,578 - Pf6N2E2_474 0.29 +0.9 539,578 - Pf6N2E2_474 0.29 +0.3 539,697 + Pf6N2E2_474 0.45 -0.9 539,705 + Pf6N2E2_474 0.46 +0.2 539,706 - Pf6N2E2_474 0.46 -0.1 540,198 + +0.8 540,198 + -1.1
Or see this region's nucleotide sequence