Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4695 and Pf6N2E2_4696 are separated by 279 nucleotides Pf6N2E2_4696 and Pf6N2E2_4697 are separated by 87 nucleotides Pf6N2E2_4697 and Pf6N2E2_4698 are separated by 142 nucleotides Pf6N2E2_4698 and Pf6N2E2_4699 are separated by 100 nucleotides
Pf6N2E2_4695 - putative secreted protein, at 5,428,333 to 5,429,502
_4695
Pf6N2E2_4696 - FIG00953108: hypothetical protein, at 5,429,782 to 5,430,198
_4696
Pf6N2E2_4697 - hypothetical protein, at 5,430,286 to 5,431,062
_4697
Pf6N2E2_4698 - putative DNA binding protein, at 5,431,205 to 5,431,531
_4698
Pf6N2E2_4699 - FIG00953563: hypothetical protein, at 5,431,632 to 5,432,033
_4699
Position (kb) 5430
5431
5432 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5429.309 kb on + strand, within Pf6N2E2_4695 at 5429.309 kb on + strand, within Pf6N2E2_4695 at 5429.310 kb on - strand, within Pf6N2E2_4695 at 5429.310 kb on - strand, within Pf6N2E2_4695 at 5429.320 kb on + strand, within Pf6N2E2_4695 at 5429.320 kb on + strand, within Pf6N2E2_4695 at 5429.321 kb on - strand, within Pf6N2E2_4695 at 5429.321 kb on - strand, within Pf6N2E2_4695 at 5429.341 kb on - strand, within Pf6N2E2_4695 at 5429.375 kb on - strand, within Pf6N2E2_4695 at 5429.455 kb on + strand at 5429.455 kb on + strand at 5429.455 kb on + strand at 5429.456 kb on - strand at 5429.456 kb on - strand at 5429.456 kb on - strand at 5429.456 kb on - strand at 5429.500 kb on + strand at 5429.500 kb on + strand at 5429.500 kb on + strand at 5429.512 kb on + strand at 5429.512 kb on + strand at 5429.513 kb on - strand at 5429.575 kb on + strand at 5429.575 kb on + strand at 5429.576 kb on - strand at 5429.576 kb on - strand at 5429.578 kb on - strand at 5429.585 kb on + strand at 5429.585 kb on + strand at 5429.586 kb on - strand at 5429.586 kb on - strand at 5429.598 kb on - strand at 5429.599 kb on + strand at 5429.599 kb on + strand at 5429.599 kb on + strand at 5429.600 kb on - strand at 5429.600 kb on - strand at 5429.600 kb on - strand at 5429.604 kb on + strand at 5429.605 kb on - strand at 5429.605 kb on - strand at 5429.605 kb on - strand at 5429.607 kb on - strand at 5429.619 kb on - strand at 5429.626 kb on + strand at 5429.627 kb on - strand at 5429.629 kb on - strand at 5429.640 kb on - strand at 5429.697 kb on + strand at 5429.698 kb on - strand at 5429.708 kb on - strand at 5429.783 kb on + strand at 5429.784 kb on - strand at 5429.816 kb on + strand at 5429.816 kb on + strand at 5429.816 kb on + strand at 5429.817 kb on - strand at 5429.817 kb on - strand at 5429.817 kb on - strand at 5429.831 kb on + strand, within Pf6N2E2_4696 at 5429.831 kb on + strand, within Pf6N2E2_4696 at 5429.832 kb on - strand, within Pf6N2E2_4696 at 5429.832 kb on - strand, within Pf6N2E2_4696 at 5429.832 kb on - strand, within Pf6N2E2_4696 at 5429.832 kb on - strand, within Pf6N2E2_4696 at 5429.832 kb on - strand, within Pf6N2E2_4696 at 5430.041 kb on - strand, within Pf6N2E2_4696 at 5430.237 kb on - strand at 5430.329 kb on + strand at 5430.329 kb on + strand at 5430.329 kb on + strand at 5430.330 kb on - strand at 5430.330 kb on - strand at 5430.380 kb on + strand, within Pf6N2E2_4697 at 5430.380 kb on + strand, within Pf6N2E2_4697 at 5430.380 kb on + strand, within Pf6N2E2_4697 at 5430.380 kb on + strand, within Pf6N2E2_4697 at 5430.380 kb on + strand, within Pf6N2E2_4697 at 5430.380 kb on + strand, within Pf6N2E2_4697 at 5430.381 kb on - strand, within Pf6N2E2_4697 at 5430.381 kb on - strand, within Pf6N2E2_4697 at 5430.441 kb on + strand, within Pf6N2E2_4697 at 5430.442 kb on - strand, within Pf6N2E2_4697 at 5430.503 kb on + strand, within Pf6N2E2_4697 at 5430.503 kb on + strand, within Pf6N2E2_4697 at 5430.504 kb on - strand, within Pf6N2E2_4697 at 5430.504 kb on - strand, within Pf6N2E2_4697 at 5430.504 kb on - strand, within Pf6N2E2_4697 at 5430.504 kb on - strand, within Pf6N2E2_4697 at 5430.504 kb on - strand, within Pf6N2E2_4697 at 5430.504 kb on - strand, within Pf6N2E2_4697 at 5430.556 kb on - strand, within Pf6N2E2_4697 at 5430.716 kb on + strand, within Pf6N2E2_4697 at 5430.716 kb on + strand, within Pf6N2E2_4697 at 5430.716 kb on + strand, within Pf6N2E2_4697 at 5430.717 kb on - strand, within Pf6N2E2_4697 at 5430.737 kb on + strand, within Pf6N2E2_4697 at 5430.737 kb on + strand, within Pf6N2E2_4697 at 5430.737 kb on + strand, within Pf6N2E2_4697 at 5430.738 kb on - strand, within Pf6N2E2_4697 at 5430.738 kb on - strand, within Pf6N2E2_4697 at 5430.738 kb on - strand, within Pf6N2E2_4697 at 5430.738 kb on - strand, within Pf6N2E2_4697 at 5430.879 kb on + strand, within Pf6N2E2_4697 at 5430.879 kb on + strand, within Pf6N2E2_4697 at 5430.880 kb on - strand, within Pf6N2E2_4697 at 5430.880 kb on - strand, within Pf6N2E2_4697 at 5430.922 kb on + strand, within Pf6N2E2_4697 at 5430.922 kb on + strand, within Pf6N2E2_4697 at 5430.922 kb on + strand, within Pf6N2E2_4697 at 5430.922 kb on + strand, within Pf6N2E2_4697 at 5430.923 kb on - strand, within Pf6N2E2_4697 at 5430.923 kb on - strand, within Pf6N2E2_4697 at 5430.923 kb on - strand, within Pf6N2E2_4697 at 5430.923 kb on - strand, within Pf6N2E2_4697 at 5430.923 kb on - strand, within Pf6N2E2_4697 at 5430.923 kb on - strand, within Pf6N2E2_4697 at 5431.017 kb on + strand at 5431.023 kb on + strand at 5431.024 kb on - strand at 5431.024 kb on - strand at 5431.024 kb on - strand at 5431.027 kb on + strand at 5431.027 kb on + strand at 5431.028 kb on - strand at 5431.028 kb on - strand at 5431.034 kb on + strand at 5431.034 kb on + strand at 5431.034 kb on + strand at 5431.035 kb on - strand at 5431.035 kb on - strand at 5431.035 kb on - strand at 5431.035 kb on - strand at 5431.035 kb on - strand at 5431.035 kb on - strand at 5431.036 kb on + strand at 5431.036 kb on + strand at 5431.036 kb on + strand at 5431.036 kb on + strand at 5431.037 kb on - strand at 5431.037 kb on - strand at 5431.037 kb on - strand at 5431.037 kb on - strand at 5431.037 kb on - strand at 5431.037 kb on - strand at 5431.062 kb on + strand at 5431.062 kb on + strand at 5431.062 kb on + strand at 5431.062 kb on + strand at 5431.063 kb on - strand at 5431.063 kb on - strand at 5431.063 kb on - strand at 5431.078 kb on + strand at 5431.079 kb on - strand at 5431.086 kb on - strand at 5431.124 kb on + strand at 5431.125 kb on - strand at 5431.125 kb on - strand at 5431.148 kb on - strand at 5431.160 kb on - strand at 5431.160 kb on - strand at 5431.160 kb on - strand at 5431.176 kb on + strand at 5431.176 kb on + strand at 5431.176 kb on + strand at 5431.176 kb on + strand at 5431.176 kb on + strand at 5431.177 kb on - strand at 5431.177 kb on - strand at 5431.177 kb on - strand at 5431.206 kb on + strand at 5431.207 kb on - strand at 5431.207 kb on - strand at 5431.231 kb on + strand at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.449 kb on + strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.450 kb on - strand, within Pf6N2E2_4698 at 5431.458 kb on + strand, within Pf6N2E2_4698 at 5431.511 kb on - strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.516 kb on + strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.517 kb on - strand at 5431.573 kb on + strand at 5431.576 kb on - strand at 5431.677 kb on + strand, within Pf6N2E2_4699 at 5431.678 kb on - strand, within Pf6N2E2_4699 at 5431.678 kb on - strand, within Pf6N2E2_4699 at 5431.678 kb on - strand, within Pf6N2E2_4699 at 5431.711 kb on + strand, within Pf6N2E2_4699 at 5431.711 kb on + strand, within Pf6N2E2_4699 at 5431.712 kb on - strand, within Pf6N2E2_4699 at 5431.712 kb on - strand, within Pf6N2E2_4699 at 5431.913 kb on - strand, within Pf6N2E2_4699
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 5,429,309 + Pf6N2E2_4695 0.83 -0.7 5,429,309 + Pf6N2E2_4695 0.83 -3.2 5,429,310 - Pf6N2E2_4695 0.84 -0.3 5,429,310 - Pf6N2E2_4695 0.84 +2.0 5,429,320 + Pf6N2E2_4695 0.84 -1.4 5,429,320 + Pf6N2E2_4695 0.84 -0.1 5,429,321 - Pf6N2E2_4695 0.84 +2.0 5,429,321 - Pf6N2E2_4695 0.84 +0.2 5,429,341 - Pf6N2E2_4695 0.86 +0.5 5,429,375 - Pf6N2E2_4695 0.89 +2.0 5,429,455 + -1.0 5,429,455 + +0.0 5,429,455 + -0.0 5,429,456 - -2.2 5,429,456 - +0.3 5,429,456 - -1.7 5,429,456 - -0.0 5,429,500 + +1.3 5,429,500 + +0.4 5,429,500 + -0.2 5,429,512 + +0.2 5,429,512 + +2.0 5,429,513 - +1.0 5,429,575 + -0.4 5,429,575 + -1.5 5,429,576 - +2.0 5,429,576 - +1.0 5,429,578 - -1.0 5,429,585 + -0.9 5,429,585 + -0.0 5,429,586 - +1.0 5,429,586 - -1.0 5,429,598 - +0.1 5,429,599 + -0.2 5,429,599 + +0.4 5,429,599 + -1.7 5,429,600 - -1.0 5,429,600 - -2.5 5,429,600 - -0.8 5,429,604 + -0.6 5,429,605 - +0.9 5,429,605 - +0.9 5,429,605 - +2.0 5,429,607 - +0.4 5,429,619 - -0.6 5,429,626 + +0.8 5,429,627 - +0.2 5,429,629 - +2.0 5,429,640 - -3.4 5,429,697 + -0.3 5,429,698 - +1.8 5,429,708 - +1.7 5,429,783 + +0.4 5,429,784 - +0.4 5,429,816 + -0.3 5,429,816 + -0.3 5,429,816 + -0.2 5,429,817 - +0.6 5,429,817 - -0.8 5,429,817 - +0.8 5,429,831 + Pf6N2E2_4696 0.12 +0.9 5,429,831 + Pf6N2E2_4696 0.12 +1.2 5,429,832 - Pf6N2E2_4696 0.12 +0.7 5,429,832 - Pf6N2E2_4696 0.12 -0.7 5,429,832 - Pf6N2E2_4696 0.12 +0.7 5,429,832 - Pf6N2E2_4696 0.12 +0.4 5,429,832 - Pf6N2E2_4696 0.12 +1.0 5,430,041 - Pf6N2E2_4696 0.62 -0.2 5,430,237 - -0.9 5,430,329 + +2.0 5,430,329 + +0.2 5,430,329 + +0.6 5,430,330 - -0.9 5,430,330 - +0.3 5,430,380 + Pf6N2E2_4697 0.12 +0.9 5,430,380 + Pf6N2E2_4697 0.12 -0.9 5,430,380 + Pf6N2E2_4697 0.12 +0.7 5,430,380 + Pf6N2E2_4697 0.12 -0.5 5,430,380 + Pf6N2E2_4697 0.12 +0.6 5,430,380 + Pf6N2E2_4697 0.12 -2.1 5,430,381 - Pf6N2E2_4697 0.12 -0.0 5,430,381 - Pf6N2E2_4697 0.12 +0.1 5,430,441 + Pf6N2E2_4697 0.20 -0.1 5,430,442 - Pf6N2E2_4697 0.20 +0.4 5,430,503 + Pf6N2E2_4697 0.28 +0.5 5,430,503 + Pf6N2E2_4697 0.28 -3.1 5,430,504 - Pf6N2E2_4697 0.28 +2.0 5,430,504 - Pf6N2E2_4697 0.28 -0.2 5,430,504 - Pf6N2E2_4697 0.28 +0.3 5,430,504 - Pf6N2E2_4697 0.28 +0.8 5,430,504 - Pf6N2E2_4697 0.28 -1.4 5,430,504 - Pf6N2E2_4697 0.28 +0.5 5,430,556 - Pf6N2E2_4697 0.35 +0.6 5,430,716 + Pf6N2E2_4697 0.55 -0.5 5,430,716 + Pf6N2E2_4697 0.55 +1.0 5,430,716 + Pf6N2E2_4697 0.55 -0.2 5,430,717 - Pf6N2E2_4697 0.55 -2.9 5,430,737 + Pf6N2E2_4697 0.58 +0.4 5,430,737 + Pf6N2E2_4697 0.58 +0.9 5,430,737 + Pf6N2E2_4697 0.58 +0.9 5,430,738 - Pf6N2E2_4697 0.58 -2.7 5,430,738 - Pf6N2E2_4697 0.58 -3.0 5,430,738 - Pf6N2E2_4697 0.58 +0.4 5,430,738 - Pf6N2E2_4697 0.58 +1.0 5,430,879 + Pf6N2E2_4697 0.76 +0.4 5,430,879 + Pf6N2E2_4697 0.76 -0.6 5,430,880 - Pf6N2E2_4697 0.76 +0.4 5,430,880 - Pf6N2E2_4697 0.76 -0.5 5,430,922 + Pf6N2E2_4697 0.82 +0.7 5,430,922 + Pf6N2E2_4697 0.82 +1.2 5,430,922 + Pf6N2E2_4697 0.82 -2.7 5,430,922 + Pf6N2E2_4697 0.82 +2.0 5,430,923 - Pf6N2E2_4697 0.82 +0.1 5,430,923 - Pf6N2E2_4697 0.82 -2.2 5,430,923 - Pf6N2E2_4697 0.82 -2.9 5,430,923 - Pf6N2E2_4697 0.82 +0.7 5,430,923 - Pf6N2E2_4697 0.82 -0.4 5,430,923 - Pf6N2E2_4697 0.82 +0.7 5,431,017 + -0.7 5,431,023 + -1.4 5,431,024 - -2.7 5,431,024 - -0.2 5,431,024 - -1.5 5,431,027 + -0.3 5,431,027 + +0.1 5,431,028 - -0.5 5,431,028 - +2.0 5,431,034 + +0.7 5,431,034 + +1.9 5,431,034 + +1.3 5,431,035 - -0.6 5,431,035 - -1.3 5,431,035 - -1.7 5,431,035 - +1.0 5,431,035 - +0.7 5,431,035 - -2.9 5,431,036 + +1.6 5,431,036 + +1.9 5,431,036 + -0.9 5,431,036 + -1.6 5,431,037 - -0.9 5,431,037 - +1.0 5,431,037 - -0.8 5,431,037 - -1.9 5,431,037 - +0.2 5,431,037 - +1.0 5,431,062 + -1.2 5,431,062 + -0.8 5,431,062 + -0.2 5,431,062 + -1.5 5,431,063 - +0.6 5,431,063 - +2.0 5,431,063 - +0.2 5,431,078 + +1.0 5,431,079 - -0.7 5,431,086 - +0.4 5,431,124 + -3.9 5,431,125 - +1.1 5,431,125 - -0.5 5,431,148 - -0.4 5,431,160 - -0.5 5,431,160 - -0.6 5,431,160 - -0.6 5,431,176 + +1.6 5,431,176 + +0.1 5,431,176 + -0.3 5,431,176 + -0.9 5,431,176 + -0.4 5,431,177 - -0.2 5,431,177 - -0.3 5,431,177 - +0.4 5,431,206 + +2.9 5,431,207 - +2.2 5,431,207 - -0.6 5,431,231 + +0.1 5,431,449 + Pf6N2E2_4698 0.75 +0.8 5,431,449 + Pf6N2E2_4698 0.75 +2.0 5,431,449 + Pf6N2E2_4698 0.75 +0.1 5,431,449 + Pf6N2E2_4698 0.75 -1.6 5,431,449 + Pf6N2E2_4698 0.75 +0.4 5,431,449 + Pf6N2E2_4698 0.75 +1.0 5,431,449 + Pf6N2E2_4698 0.75 +0.0 5,431,449 + Pf6N2E2_4698 0.75 +1.0 5,431,449 + Pf6N2E2_4698 0.75 -0.4 5,431,449 + Pf6N2E2_4698 0.75 -2.1 5,431,449 + Pf6N2E2_4698 0.75 +0.4 5,431,450 - Pf6N2E2_4698 0.75 -0.3 5,431,450 - Pf6N2E2_4698 0.75 -0.5 5,431,450 - Pf6N2E2_4698 0.75 -1.0 5,431,450 - Pf6N2E2_4698 0.75 -1.2 5,431,450 - Pf6N2E2_4698 0.75 +0.9 5,431,458 + Pf6N2E2_4698 0.77 -1.1 5,431,511 - +0.0 5,431,516 + +2.0 5,431,516 + -3.1 5,431,516 + -0.5 5,431,516 + +2.0 5,431,516 + +0.2 5,431,516 + -0.8 5,431,517 - +1.6 5,431,517 - -1.5 5,431,517 - +0.3 5,431,517 - -2.4 5,431,517 - +0.1 5,431,517 - -0.4 5,431,517 - +2.0 5,431,517 - +0.3 5,431,517 - +1.5 5,431,517 - +1.1 5,431,573 + +0.1 5,431,576 - +0.9 5,431,677 + Pf6N2E2_4699 0.11 +0.3 5,431,678 - Pf6N2E2_4699 0.11 +0.1 5,431,678 - Pf6N2E2_4699 0.11 +0.7 5,431,678 - Pf6N2E2_4699 0.11 -1.1 5,431,711 + Pf6N2E2_4699 0.20 +0.7 5,431,711 + Pf6N2E2_4699 0.20 +0.6 5,431,712 - Pf6N2E2_4699 0.20 -0.4 5,431,712 - Pf6N2E2_4699 0.20 -0.6 5,431,913 - Pf6N2E2_4699 0.70 -0.2
Or see this region's nucleotide sequence