Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2873 and Pf6N2E2_2874 are separated by 24 nucleotides Pf6N2E2_2874 and Pf6N2E2_2875 are separated by 71 nucleotides
Pf6N2E2_2873 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 3,361,704 to 3,363,392
_2873
Pf6N2E2_2874 - hypothetical protein, at 3,363,417 to 3,363,545
_2874
Pf6N2E2_2875 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative, at 3,363,617 to 3,364,561
_2875
Position (kb)
3363
3364 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.581 kb on + strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.582 kb on - strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.585 kb on + strand, within Pf6N2E2_2873 at 3362.586 kb on - strand, within Pf6N2E2_2873 at 3362.586 kb on - strand, within Pf6N2E2_2873 at 3362.586 kb on - strand, within Pf6N2E2_2873 at 3362.586 kb on - strand, within Pf6N2E2_2873 at 3362.586 kb on - strand, within Pf6N2E2_2873 at 3362.586 kb on - strand, within Pf6N2E2_2873 at 3362.586 kb on - strand, within Pf6N2E2_2873 at 3362.590 kb on + strand, within Pf6N2E2_2873 at 3362.590 kb on + strand, within Pf6N2E2_2873 at 3362.591 kb on - strand, within Pf6N2E2_2873 at 3362.752 kb on + strand, within Pf6N2E2_2873 at 3362.753 kb on - strand, within Pf6N2E2_2873 at 3362.753 kb on - strand, within Pf6N2E2_2873 at 3362.753 kb on - strand, within Pf6N2E2_2873 at 3362.753 kb on - strand, within Pf6N2E2_2873 at 3362.768 kb on + strand, within Pf6N2E2_2873 at 3362.857 kb on + strand, within Pf6N2E2_2873 at 3362.858 kb on - strand, within Pf6N2E2_2873 at 3362.858 kb on - strand, within Pf6N2E2_2873 at 3363.010 kb on + strand, within Pf6N2E2_2873 at 3363.010 kb on + strand, within Pf6N2E2_2873 at 3363.010 kb on + strand, within Pf6N2E2_2873 at 3363.011 kb on - strand, within Pf6N2E2_2873 at 3363.011 kb on - strand, within Pf6N2E2_2873 at 3363.067 kb on + strand, within Pf6N2E2_2873 at 3363.067 kb on + strand, within Pf6N2E2_2873 at 3363.067 kb on + strand, within Pf6N2E2_2873 at 3363.068 kb on - strand, within Pf6N2E2_2873 at 3363.068 kb on - strand, within Pf6N2E2_2873 at 3363.068 kb on - strand, within Pf6N2E2_2873 at 3363.068 kb on - strand, within Pf6N2E2_2873 at 3363.220 kb on + strand, within Pf6N2E2_2873 at 3363.238 kb on + strand at 3363.238 kb on + strand at 3363.238 kb on + strand at 3363.238 kb on + strand at 3363.239 kb on - strand at 3363.239 kb on - strand at 3363.239 kb on - strand at 3363.325 kb on + strand at 3363.409 kb on - strand at 3363.409 kb on - strand at 3363.436 kb on - strand, within Pf6N2E2_2874 at 3363.467 kb on + strand, within Pf6N2E2_2874 at 3363.467 kb on + strand, within Pf6N2E2_2874 at 3363.467 kb on + strand, within Pf6N2E2_2874 at 3363.467 kb on + strand, within Pf6N2E2_2874 at 3363.467 kb on + strand, within Pf6N2E2_2874 at 3363.468 kb on - strand, within Pf6N2E2_2874 at 3363.468 kb on - strand, within Pf6N2E2_2874 at 3363.468 kb on - strand, within Pf6N2E2_2874 at 3363.468 kb on - strand, within Pf6N2E2_2874 at 3363.474 kb on + strand, within Pf6N2E2_2874 at 3363.475 kb on - strand, within Pf6N2E2_2874 at 3363.492 kb on + strand, within Pf6N2E2_2874 at 3363.492 kb on + strand, within Pf6N2E2_2874 at 3363.492 kb on + strand, within Pf6N2E2_2874 at 3363.492 kb on + strand, within Pf6N2E2_2874 at 3363.492 kb on + strand, within Pf6N2E2_2874 at 3363.492 kb on + strand, within Pf6N2E2_2874 at 3363.493 kb on - strand, within Pf6N2E2_2874 at 3363.493 kb on - strand, within Pf6N2E2_2874 at 3363.493 kb on - strand, within Pf6N2E2_2874 at 3363.509 kb on + strand, within Pf6N2E2_2874 at 3363.509 kb on + strand, within Pf6N2E2_2874 at 3363.509 kb on + strand, within Pf6N2E2_2874 at 3363.510 kb on - strand, within Pf6N2E2_2874 at 3363.510 kb on - strand, within Pf6N2E2_2874 at 3363.510 kb on - strand, within Pf6N2E2_2874 at 3363.510 kb on - strand, within Pf6N2E2_2874 at 3363.510 kb on - strand, within Pf6N2E2_2874 at 3363.510 kb on - strand, within Pf6N2E2_2874 at 3363.510 kb on - strand, within Pf6N2E2_2874 at 3363.538 kb on + strand at 3363.570 kb on + strand at 3363.570 kb on + strand at 3363.570 kb on + strand at 3363.572 kb on + strand at 3363.572 kb on + strand at 3363.572 kb on + strand at 3363.572 kb on + strand at 3363.572 kb on + strand at 3363.572 kb on + strand at 3363.572 kb on + strand at 3363.573 kb on - strand at 3363.573 kb on - strand at 3363.573 kb on - strand at 3363.573 kb on - strand at 3363.573 kb on - strand at 3363.573 kb on - strand at 3363.782 kb on + strand, within Pf6N2E2_2875 at 3363.783 kb on - strand, within Pf6N2E2_2875 at 3363.907 kb on + strand, within Pf6N2E2_2875 at 3363.908 kb on - strand, within Pf6N2E2_2875 at 3363.965 kb on + strand, within Pf6N2E2_2875 at 3363.965 kb on + strand, within Pf6N2E2_2875 at 3363.965 kb on + strand, within Pf6N2E2_2875 at 3363.966 kb on - strand, within Pf6N2E2_2875 at 3363.966 kb on - strand, within Pf6N2E2_2875 at 3363.966 kb on - strand, within Pf6N2E2_2875 at 3363.980 kb on + strand, within Pf6N2E2_2875 at 3363.981 kb on - strand, within Pf6N2E2_2875 at 3363.981 kb on - strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.217 kb on + strand, within Pf6N2E2_2875 at 3364.218 kb on - strand, within Pf6N2E2_2875 at 3364.218 kb on - strand, within Pf6N2E2_2875 at 3364.218 kb on - strand, within Pf6N2E2_2875 at 3364.218 kb on - strand, within Pf6N2E2_2875 at 3364.218 kb on - strand, within Pf6N2E2_2875 at 3364.218 kb on - strand, within Pf6N2E2_2875 at 3364.218 kb on - strand, within Pf6N2E2_2875
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 3,362,581 + Pf6N2E2_2873 0.52 +2.0 3,362,581 + Pf6N2E2_2873 0.52 -2.7 3,362,581 + Pf6N2E2_2873 0.52 +0.9 3,362,581 + Pf6N2E2_2873 0.52 +0.6 3,362,581 + Pf6N2E2_2873 0.52 -1.0 3,362,581 + Pf6N2E2_2873 0.52 -0.9 3,362,581 + Pf6N2E2_2873 0.52 -0.9 3,362,581 + Pf6N2E2_2873 0.52 -1.4 3,362,582 - Pf6N2E2_2873 0.52 +0.3 3,362,582 - Pf6N2E2_2873 0.52 -0.2 3,362,582 - Pf6N2E2_2873 0.52 +0.4 3,362,582 - Pf6N2E2_2873 0.52 +1.2 3,362,582 - Pf6N2E2_2873 0.52 +0.2 3,362,582 - Pf6N2E2_2873 0.52 -0.3 3,362,582 - Pf6N2E2_2873 0.52 -1.5 3,362,582 - Pf6N2E2_2873 0.52 +2.0 3,362,582 - Pf6N2E2_2873 0.52 +2.0 3,362,582 - Pf6N2E2_2873 0.52 +0.5 3,362,585 + Pf6N2E2_2873 0.52 -0.8 3,362,585 + Pf6N2E2_2873 0.52 -0.8 3,362,585 + Pf6N2E2_2873 0.52 +0.5 3,362,585 + Pf6N2E2_2873 0.52 -1.3 3,362,585 + Pf6N2E2_2873 0.52 -2.2 3,362,585 + Pf6N2E2_2873 0.52 -0.0 3,362,585 + Pf6N2E2_2873 0.52 -1.4 3,362,585 + Pf6N2E2_2873 0.52 -2.8 3,362,585 + Pf6N2E2_2873 0.52 -0.8 3,362,586 - Pf6N2E2_2873 0.52 -1.2 3,362,586 - Pf6N2E2_2873 0.52 -0.3 3,362,586 - Pf6N2E2_2873 0.52 +0.2 3,362,586 - Pf6N2E2_2873 0.52 -0.4 3,362,586 - Pf6N2E2_2873 0.52 +2.0 3,362,586 - Pf6N2E2_2873 0.52 +1.0 3,362,586 - Pf6N2E2_2873 0.52 -0.6 3,362,590 + Pf6N2E2_2873 0.52 -1.4 3,362,590 + Pf6N2E2_2873 0.52 +0.5 3,362,591 - Pf6N2E2_2873 0.53 -0.5 3,362,752 + Pf6N2E2_2873 0.62 -1.5 3,362,753 - Pf6N2E2_2873 0.62 -1.4 3,362,753 - Pf6N2E2_2873 0.62 +2.0 3,362,753 - Pf6N2E2_2873 0.62 +0.9 3,362,753 - Pf6N2E2_2873 0.62 -2.4 3,362,768 + Pf6N2E2_2873 0.63 -0.1 3,362,857 + Pf6N2E2_2873 0.68 -0.9 3,362,858 - Pf6N2E2_2873 0.68 +0.6 3,362,858 - Pf6N2E2_2873 0.68 -1.1 3,363,010 + Pf6N2E2_2873 0.77 -0.6 3,363,010 + Pf6N2E2_2873 0.77 -0.5 3,363,010 + Pf6N2E2_2873 0.77 +2.0 3,363,011 - Pf6N2E2_2873 0.77 -0.2 3,363,011 - Pf6N2E2_2873 0.77 -1.9 3,363,067 + Pf6N2E2_2873 0.81 +2.5 3,363,067 + Pf6N2E2_2873 0.81 +1.0 3,363,067 + Pf6N2E2_2873 0.81 -1.1 3,363,068 - Pf6N2E2_2873 0.81 +0.5 3,363,068 - Pf6N2E2_2873 0.81 +0.5 3,363,068 - Pf6N2E2_2873 0.81 +2.0 3,363,068 - Pf6N2E2_2873 0.81 +2.3 3,363,220 + Pf6N2E2_2873 0.90 +1.2 3,363,238 + -1.1 3,363,238 + +0.8 3,363,238 + -0.6 3,363,238 + -2.5 3,363,239 - +1.0 3,363,239 - +2.0 3,363,239 - -0.0 3,363,325 + -1.7 3,363,409 - +0.8 3,363,409 - -0.1 3,363,436 - Pf6N2E2_2874 0.15 +2.0 3,363,467 + Pf6N2E2_2874 0.39 -0.0 3,363,467 + Pf6N2E2_2874 0.39 +0.5 3,363,467 + Pf6N2E2_2874 0.39 -0.2 3,363,467 + Pf6N2E2_2874 0.39 -0.9 3,363,467 + Pf6N2E2_2874 0.39 -0.4 3,363,468 - Pf6N2E2_2874 0.40 -0.8 3,363,468 - Pf6N2E2_2874 0.40 +1.0 3,363,468 - Pf6N2E2_2874 0.40 +0.0 3,363,468 - Pf6N2E2_2874 0.40 +0.0 3,363,474 + Pf6N2E2_2874 0.44 -0.3 3,363,475 - Pf6N2E2_2874 0.45 -0.2 3,363,492 + Pf6N2E2_2874 0.58 -0.3 3,363,492 + Pf6N2E2_2874 0.58 -3.2 3,363,492 + Pf6N2E2_2874 0.58 +0.3 3,363,492 + Pf6N2E2_2874 0.58 -0.5 3,363,492 + Pf6N2E2_2874 0.58 -2.8 3,363,492 + Pf6N2E2_2874 0.58 +1.0 3,363,493 - Pf6N2E2_2874 0.59 +0.1 3,363,493 - Pf6N2E2_2874 0.59 +0.0 3,363,493 - Pf6N2E2_2874 0.59 -0.5 3,363,509 + Pf6N2E2_2874 0.71 -1.5 3,363,509 + Pf6N2E2_2874 0.71 -2.0 3,363,509 + Pf6N2E2_2874 0.71 +0.5 3,363,510 - Pf6N2E2_2874 0.72 -2.0 3,363,510 - Pf6N2E2_2874 0.72 +0.0 3,363,510 - Pf6N2E2_2874 0.72 +1.0 3,363,510 - Pf6N2E2_2874 0.72 -0.5 3,363,510 - Pf6N2E2_2874 0.72 -0.5 3,363,510 - Pf6N2E2_2874 0.72 -0.9 3,363,510 - Pf6N2E2_2874 0.72 +1.0 3,363,538 + +1.0 3,363,570 + -1.0 3,363,570 + -0.5 3,363,570 + -0.7 3,363,572 + +2.0 3,363,572 + +2.0 3,363,572 + -2.5 3,363,572 + +2.0 3,363,572 + +2.0 3,363,572 + -0.3 3,363,572 + -1.5 3,363,573 - -1.4 3,363,573 - -0.5 3,363,573 - -0.5 3,363,573 - +1.7 3,363,573 - -2.0 3,363,573 - +0.4 3,363,782 + Pf6N2E2_2875 0.17 -0.1 3,363,783 - Pf6N2E2_2875 0.18 -0.0 3,363,907 + Pf6N2E2_2875 0.31 -0.1 3,363,908 - Pf6N2E2_2875 0.31 -0.6 3,363,965 + Pf6N2E2_2875 0.37 +2.0 3,363,965 + Pf6N2E2_2875 0.37 +0.6 3,363,965 + Pf6N2E2_2875 0.37 +0.1 3,363,966 - Pf6N2E2_2875 0.37 -1.0 3,363,966 - Pf6N2E2_2875 0.37 -1.5 3,363,966 - Pf6N2E2_2875 0.37 -0.3 3,363,980 + Pf6N2E2_2875 0.38 +2.0 3,363,981 - Pf6N2E2_2875 0.39 +2.0 3,363,981 - Pf6N2E2_2875 0.39 -0.4 3,364,217 + Pf6N2E2_2875 0.63 +2.0 3,364,217 + Pf6N2E2_2875 0.63 +0.0 3,364,217 + Pf6N2E2_2875 0.63 +0.1 3,364,217 + Pf6N2E2_2875 0.63 -1.1 3,364,217 + Pf6N2E2_2875 0.63 -0.8 3,364,217 + Pf6N2E2_2875 0.63 -1.3 3,364,217 + Pf6N2E2_2875 0.63 -3.2 3,364,217 + Pf6N2E2_2875 0.63 -2.3 3,364,218 - Pf6N2E2_2875 0.64 +1.0 3,364,218 - Pf6N2E2_2875 0.64 +0.9 3,364,218 - Pf6N2E2_2875 0.64 -1.1 3,364,218 - Pf6N2E2_2875 0.64 -0.3 3,364,218 - Pf6N2E2_2875 0.64 +0.5 3,364,218 - Pf6N2E2_2875 0.64 -1.5 3,364,218 - Pf6N2E2_2875 0.64 -1.8
Or see this region's nucleotide sequence