Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1714 and Pf6N2E2_1715 are separated by 1 nucleotides Pf6N2E2_1715 and Pf6N2E2_1716 are separated by 8 nucleotides Pf6N2E2_1716 and Pf6N2E2_1717 are separated by 651 nucleotides
Pf6N2E2_1714 - FIG01218476: hypothetical protein, at 2,022,370 to 2,023,053
_1714
Pf6N2E2_1715 - hypothetical protein, at 2,023,055 to 2,023,600
_1715
Pf6N2E2_1716 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 2,023,609 to 2,024,961
_1716
Pf6N2E2_1717 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 2,025,613 to 2,027,082
_1717
Position (kb)
2023
2024
2025 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2022.638 kb on + strand, within Pf6N2E2_1714 at 2022.662 kb on + strand, within Pf6N2E2_1714 at 2022.912 kb on - strand, within Pf6N2E2_1714 at 2022.912 kb on - strand, within Pf6N2E2_1714 at 2022.917 kb on + strand, within Pf6N2E2_1714 at 2022.942 kb on - strand, within Pf6N2E2_1714 at 2023.102 kb on - strand at 2023.182 kb on + strand, within Pf6N2E2_1715 at 2023.183 kb on - strand, within Pf6N2E2_1715 at 2023.446 kb on + strand, within Pf6N2E2_1715 at 2023.447 kb on - strand, within Pf6N2E2_1715 at 2023.527 kb on + strand, within Pf6N2E2_1715 at 2024.129 kb on + strand, within Pf6N2E2_1716 at 2024.129 kb on + strand, within Pf6N2E2_1716 at 2024.130 kb on - strand, within Pf6N2E2_1716 at 2024.130 kb on - strand, within Pf6N2E2_1716 at 2024.130 kb on - strand, within Pf6N2E2_1716 at 2024.130 kb on - strand, within Pf6N2E2_1716 at 2024.130 kb on - strand, within Pf6N2E2_1716 at 2024.130 kb on - strand, within Pf6N2E2_1716 at 2024.237 kb on + strand, within Pf6N2E2_1716 at 2024.517 kb on + strand, within Pf6N2E2_1716 at 2024.610 kb on - strand, within Pf6N2E2_1716 at 2024.672 kb on + strand, within Pf6N2E2_1716 at 2024.907 kb on - strand at 2025.025 kb on - strand at 2025.027 kb on - strand at 2025.093 kb on - strand at 2025.097 kb on - strand at 2025.109 kb on - strand at 2025.347 kb on + strand at 2025.606 kb on - strand at 2025.683 kb on + strand at 2025.685 kb on + strand at 2025.914 kb on + strand, within Pf6N2E2_1717
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 2,022,638 + Pf6N2E2_1714 0.39 -0.4 2,022,662 + Pf6N2E2_1714 0.43 +0.1 2,022,912 - Pf6N2E2_1714 0.79 -0.4 2,022,912 - Pf6N2E2_1714 0.79 -2.3 2,022,917 + Pf6N2E2_1714 0.80 +0.1 2,022,942 - Pf6N2E2_1714 0.84 -0.1 2,023,102 - -1.0 2,023,182 + Pf6N2E2_1715 0.23 +1.4 2,023,183 - Pf6N2E2_1715 0.23 -2.7 2,023,446 + Pf6N2E2_1715 0.72 -0.3 2,023,447 - Pf6N2E2_1715 0.72 +0.1 2,023,527 + Pf6N2E2_1715 0.86 -1.5 2,024,129 + Pf6N2E2_1716 0.38 -1.6 2,024,129 + Pf6N2E2_1716 0.38 -0.9 2,024,130 - Pf6N2E2_1716 0.39 +1.9 2,024,130 - Pf6N2E2_1716 0.39 +1.9 2,024,130 - Pf6N2E2_1716 0.39 -1.4 2,024,130 - Pf6N2E2_1716 0.39 -1.5 2,024,130 - Pf6N2E2_1716 0.39 -0.7 2,024,130 - Pf6N2E2_1716 0.39 -0.1 2,024,237 + Pf6N2E2_1716 0.46 -0.1 2,024,517 + Pf6N2E2_1716 0.67 -0.1 2,024,610 - Pf6N2E2_1716 0.74 -0.1 2,024,672 + Pf6N2E2_1716 0.79 -1.4 2,024,907 - -0.3 2,025,025 - -1.5 2,025,027 - -1.4 2,025,093 - +0.9 2,025,097 - -1.4 2,025,109 - -3.4 2,025,347 + +1.9 2,025,606 - -0.6 2,025,683 + +1.9 2,025,685 + +0.1 2,025,914 + Pf6N2E2_1717 0.20 -0.1
Or see this region's nucleotide sequence