Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4034 and Pf6N2E2_6130 are separated by 172 nucleotides Pf6N2E2_6130 and Pf6N2E2_6131 are separated by 103 nucleotides Pf6N2E2_6131 and Pf6N2E2_6132 are separated by 167 nucleotides
Pf6N2E2_4034 - NADH:ubiquinone oxidoreductase subunit 2 (chain N), at 4,708,736 to 4,709,374
_4034
Pf6N2E2_6130 - 5S RNA, at 4,709,547 to 4,709,666
_6130
Pf6N2E2_6131 - 5S RNA, at 4,709,770 to 4,709,889
_6131
Pf6N2E2_6132 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 4,710,057 to 4,712,948
_6132
Position (kb)
4709
4710 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4708.556 kb on + strand at 4708.556 kb on + strand at 4708.556 kb on + strand at 4708.557 kb on - strand at 4708.557 kb on - strand at 4708.557 kb on - strand at 4708.557 kb on - strand at 4708.570 kb on + strand at 4708.571 kb on - strand at 4708.571 kb on - strand at 4708.571 kb on - strand at 4708.571 kb on - strand at 4708.682 kb on + strand at 4708.703 kb on + strand at 4708.704 kb on - strand at 4708.712 kb on - strand at 4708.737 kb on + strand at 4708.737 kb on + strand at 4708.803 kb on + strand, within Pf6N2E2_4034 at 4708.803 kb on + strand, within Pf6N2E2_4034 at 4708.804 kb on - strand, within Pf6N2E2_4034 at 4709.074 kb on - strand, within Pf6N2E2_4034 at 4709.074 kb on - strand, within Pf6N2E2_4034 at 4709.155 kb on + strand, within Pf6N2E2_4034 at 4709.155 kb on + strand, within Pf6N2E2_4034 at 4709.155 kb on + strand, within Pf6N2E2_4034 at 4709.155 kb on + strand, within Pf6N2E2_4034 at 4709.155 kb on + strand, within Pf6N2E2_4034 at 4709.156 kb on - strand, within Pf6N2E2_4034 at 4709.156 kb on - strand, within Pf6N2E2_4034 at 4709.156 kb on - strand, within Pf6N2E2_4034 at 4709.301 kb on + strand, within Pf6N2E2_4034 at 4709.301 kb on + strand, within Pf6N2E2_4034 at 4709.301 kb on + strand, within Pf6N2E2_4034 at 4709.301 kb on + strand, within Pf6N2E2_4034 at 4709.302 kb on - strand, within Pf6N2E2_4034 at 4709.302 kb on - strand, within Pf6N2E2_4034 at 4709.307 kb on + strand, within Pf6N2E2_4034 at 4709.307 kb on + strand, within Pf6N2E2_4034 at 4709.307 kb on + strand, within Pf6N2E2_4034 at 4709.308 kb on - strand, within Pf6N2E2_4034 at 4709.308 kb on - strand, within Pf6N2E2_4034 at 4709.308 kb on - strand, within Pf6N2E2_4034 at 4709.430 kb on + strand at 4709.440 kb on + strand at 4709.440 kb on + strand at 4709.440 kb on + strand at 4709.440 kb on + strand at 4709.440 kb on + strand at 4709.440 kb on + strand at 4709.440 kb on + strand at 4709.440 kb on + strand at 4709.441 kb on - strand at 4709.441 kb on - strand at 4709.441 kb on - strand at 4709.441 kb on - strand at 4709.564 kb on - strand, within Pf6N2E2_6130 at 4709.564 kb on - strand, within Pf6N2E2_6130 at 4709.564 kb on - strand, within Pf6N2E2_6130 at 4709.564 kb on - strand, within Pf6N2E2_6130 at 4709.564 kb on - strand, within Pf6N2E2_6130 at 4709.590 kb on - strand, within Pf6N2E2_6130 at 4709.590 kb on - strand, within Pf6N2E2_6130 at 4709.590 kb on - strand, within Pf6N2E2_6130 at 4709.652 kb on + strand, within Pf6N2E2_6130 at 4709.719 kb on + strand at 4709.738 kb on - strand at 4709.738 kb on - strand at 4709.754 kb on - strand at 4709.754 kb on - strand at 4709.754 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 4,708,556 + -0.1 4,708,556 + -1.5 4,708,556 + -0.4 4,708,557 - -0.1 4,708,557 - +0.5 4,708,557 - -0.1 4,708,557 - -0.4 4,708,570 + -0.3 4,708,571 - -1.9 4,708,571 - +0.4 4,708,571 - +0.4 4,708,571 - -0.8 4,708,682 + -0.0 4,708,703 + +2.0 4,708,704 - -0.7 4,708,712 - -1.7 4,708,737 + -0.7 4,708,737 + -1.6 4,708,803 + Pf6N2E2_4034 0.10 -1.3 4,708,803 + Pf6N2E2_4034 0.10 -1.2 4,708,804 - Pf6N2E2_4034 0.11 +1.2 4,709,074 - Pf6N2E2_4034 0.53 -0.1 4,709,074 - Pf6N2E2_4034 0.53 +0.8 4,709,155 + Pf6N2E2_4034 0.66 -0.9 4,709,155 + Pf6N2E2_4034 0.66 +1.1 4,709,155 + Pf6N2E2_4034 0.66 +1.0 4,709,155 + Pf6N2E2_4034 0.66 +2.0 4,709,155 + Pf6N2E2_4034 0.66 +0.3 4,709,156 - Pf6N2E2_4034 0.66 -0.3 4,709,156 - Pf6N2E2_4034 0.66 +0.1 4,709,156 - Pf6N2E2_4034 0.66 -0.9 4,709,301 + Pf6N2E2_4034 0.88 +1.2 4,709,301 + Pf6N2E2_4034 0.88 +0.4 4,709,301 + Pf6N2E2_4034 0.88 +1.2 4,709,301 + Pf6N2E2_4034 0.88 -0.8 4,709,302 - Pf6N2E2_4034 0.89 +0.8 4,709,302 - Pf6N2E2_4034 0.89 -2.3 4,709,307 + Pf6N2E2_4034 0.89 +0.4 4,709,307 + Pf6N2E2_4034 0.89 +0.5 4,709,307 + Pf6N2E2_4034 0.89 -1.9 4,709,308 - Pf6N2E2_4034 0.90 -0.6 4,709,308 - Pf6N2E2_4034 0.90 +0.2 4,709,308 - Pf6N2E2_4034 0.90 +1.3 4,709,430 + +0.5 4,709,440 + -0.7 4,709,440 + +1.0 4,709,440 + +1.3 4,709,440 + -0.8 4,709,440 + -0.9 4,709,440 + +1.0 4,709,440 + -0.5 4,709,440 + +0.2 4,709,441 - -0.3 4,709,441 - -0.7 4,709,441 - +2.0 4,709,441 - -0.2 4,709,564 - Pf6N2E2_6130 0.14 -0.2 4,709,564 - Pf6N2E2_6130 0.14 +2.0 4,709,564 - Pf6N2E2_6130 0.14 -0.8 4,709,564 - Pf6N2E2_6130 0.14 -0.8 4,709,564 - Pf6N2E2_6130 0.14 -1.3 4,709,590 - Pf6N2E2_6130 0.36 +2.0 4,709,590 - Pf6N2E2_6130 0.36 -0.3 4,709,590 - Pf6N2E2_6130 0.36 -2.4 4,709,652 + Pf6N2E2_6130 0.88 -0.0 4,709,719 + -0.7 4,709,738 - -0.7 4,709,738 - +2.0 4,709,754 - -1.7 4,709,754 - +1.3 4,709,754 - +2.0
Or see this region's nucleotide sequence