Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4502 and Pf6N2E2_4503 are separated by 88 nucleotides Pf6N2E2_4503 and Pf6N2E2_4504 are separated by 24 nucleotides
Pf6N2E2_4502 - FMN reductase (EC 1.5.1.29), at 5,234,449 to 5,235,042
_4502
Pf6N2E2_4503 - Alkanesulfonates-binding protein, at 5,235,131 to 5,236,096
_4503
Pf6N2E2_4504 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 5,236,121 to 5,237,269
_4504
Position (kb)
5235
5236
5237 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5234.255 kb on + strand at 5234.256 kb on - strand at 5234.296 kb on + strand at 5234.296 kb on + strand at 5234.297 kb on - strand at 5234.303 kb on + strand at 5234.303 kb on + strand at 5234.303 kb on + strand at 5234.304 kb on - strand at 5234.304 kb on - strand at 5234.304 kb on - strand at 5234.369 kb on - strand at 5234.382 kb on + strand at 5234.396 kb on + strand at 5234.397 kb on - strand at 5234.397 kb on - strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.434 kb on + strand at 5234.441 kb on + strand at 5234.441 kb on + strand at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.557 kb on + strand, within Pf6N2E2_4502 at 5234.558 kb on - strand, within Pf6N2E2_4502 at 5234.558 kb on - strand, within Pf6N2E2_4502 at 5234.558 kb on - strand, within Pf6N2E2_4502 at 5234.677 kb on + strand, within Pf6N2E2_4502 at 5234.678 kb on - strand, within Pf6N2E2_4502 at 5234.678 kb on - strand, within Pf6N2E2_4502 at 5234.711 kb on + strand, within Pf6N2E2_4502 at 5234.746 kb on - strand, within Pf6N2E2_4502 at 5234.787 kb on + strand, within Pf6N2E2_4502 at 5234.803 kb on + strand, within Pf6N2E2_4502 at 5234.804 kb on - strand, within Pf6N2E2_4502 at 5235.002 kb on + strand at 5235.069 kb on + strand at 5235.069 kb on + strand at 5235.069 kb on + strand at 5235.069 kb on + strand at 5235.070 kb on - strand at 5235.070 kb on - strand at 5235.199 kb on + strand at 5235.199 kb on + strand at 5235.200 kb on - strand at 5235.200 kb on - strand at 5235.200 kb on - strand at 5235.437 kb on + strand, within Pf6N2E2_4503 at 5235.437 kb on + strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.438 kb on - strand, within Pf6N2E2_4503 at 5235.446 kb on + strand, within Pf6N2E2_4503 at 5235.446 kb on + strand, within Pf6N2E2_4503 at 5235.447 kb on - strand, within Pf6N2E2_4503 at 5235.447 kb on - strand, within Pf6N2E2_4503 at 5235.447 kb on - strand, within Pf6N2E2_4503 at 5235.564 kb on - strand, within Pf6N2E2_4503 at 5235.564 kb on - strand, within Pf6N2E2_4503 at 5235.602 kb on + strand, within Pf6N2E2_4503 at 5235.773 kb on + strand, within Pf6N2E2_4503 at 5235.774 kb on - strand, within Pf6N2E2_4503 at 5235.792 kb on - strand, within Pf6N2E2_4503 at 5235.891 kb on + strand, within Pf6N2E2_4503 at 5236.013 kb on + strand at 5236.013 kb on + strand at 5236.013 kb on + strand at 5236.013 kb on + strand at 5236.013 kb on + strand at 5236.014 kb on - strand at 5236.014 kb on - strand at 5236.014 kb on - strand at 5236.014 kb on - strand at 5236.094 kb on + strand at 5236.094 kb on + strand at 5236.094 kb on + strand at 5236.094 kb on + strand at 5236.095 kb on - strand at 5236.132 kb on + strand at 5236.169 kb on + strand at 5236.169 kb on + strand at 5236.169 kb on + strand at 5236.532 kb on - strand, within Pf6N2E2_4504 at 5236.568 kb on + strand, within Pf6N2E2_4504 at 5236.569 kb on - strand, within Pf6N2E2_4504 at 5236.591 kb on + strand, within Pf6N2E2_4504 at 5236.785 kb on + strand, within Pf6N2E2_4504 at 5237.075 kb on + strand, within Pf6N2E2_4504 at 5237.076 kb on - strand, within Pf6N2E2_4504
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 5,234,255 + -0.3 5,234,256 - -1.4 5,234,296 + -1.1 5,234,296 + -0.7 5,234,297 - -0.3 5,234,303 + +1.1 5,234,303 + -1.5 5,234,303 + +2.1 5,234,304 - -0.9 5,234,304 - -1.5 5,234,304 - -0.5 5,234,369 - -2.6 5,234,382 + +0.3 5,234,396 + +0.1 5,234,397 - -0.9 5,234,397 - -1.5 5,234,434 + +0.0 5,234,434 + -0.9 5,234,434 + -1.5 5,234,434 + -0.8 5,234,434 + -0.7 5,234,441 + -1.2 5,234,441 + +0.5 5,234,557 + Pf6N2E2_4502 0.18 -1.0 5,234,557 + Pf6N2E2_4502 0.18 -0.7 5,234,557 + Pf6N2E2_4502 0.18 +2.1 5,234,557 + Pf6N2E2_4502 0.18 -1.4 5,234,557 + Pf6N2E2_4502 0.18 -0.1 5,234,558 - Pf6N2E2_4502 0.18 -1.4 5,234,558 - Pf6N2E2_4502 0.18 +2.1 5,234,558 - Pf6N2E2_4502 0.18 -1.5 5,234,677 + Pf6N2E2_4502 0.38 +0.5 5,234,678 - Pf6N2E2_4502 0.39 +1.1 5,234,678 - Pf6N2E2_4502 0.39 +1.3 5,234,711 + Pf6N2E2_4502 0.44 +1.4 5,234,746 - Pf6N2E2_4502 0.50 -0.6 5,234,787 + Pf6N2E2_4502 0.57 -1.3 5,234,803 + Pf6N2E2_4502 0.60 -0.8 5,234,804 - Pf6N2E2_4502 0.60 -2.6 5,235,002 + +1.1 5,235,069 + +2.1 5,235,069 + -0.0 5,235,069 + -1.4 5,235,069 + +1.0 5,235,070 - -2.3 5,235,070 - -1.3 5,235,199 + -0.5 5,235,199 + -0.3 5,235,200 - +2.1 5,235,200 - -0.7 5,235,200 - -0.4 5,235,437 + Pf6N2E2_4503 0.32 -1.5 5,235,437 + Pf6N2E2_4503 0.32 -1.9 5,235,438 - Pf6N2E2_4503 0.32 +0.1 5,235,438 - Pf6N2E2_4503 0.32 -1.1 5,235,438 - Pf6N2E2_4503 0.32 -0.6 5,235,438 - Pf6N2E2_4503 0.32 -0.4 5,235,438 - Pf6N2E2_4503 0.32 -0.9 5,235,438 - Pf6N2E2_4503 0.32 -1.4 5,235,438 - Pf6N2E2_4503 0.32 -1.0 5,235,438 - Pf6N2E2_4503 0.32 -2.7 5,235,438 - Pf6N2E2_4503 0.32 -1.7 5,235,438 - Pf6N2E2_4503 0.32 -1.4 5,235,446 + Pf6N2E2_4503 0.33 +1.1 5,235,446 + Pf6N2E2_4503 0.33 +1.2 5,235,447 - Pf6N2E2_4503 0.33 +0.1 5,235,447 - Pf6N2E2_4503 0.33 -4.0 5,235,447 - Pf6N2E2_4503 0.33 -1.0 5,235,564 - Pf6N2E2_4503 0.45 +0.4 5,235,564 - Pf6N2E2_4503 0.45 -0.5 5,235,602 + Pf6N2E2_4503 0.49 -2.7 5,235,773 + Pf6N2E2_4503 0.66 +0.5 5,235,774 - Pf6N2E2_4503 0.67 +0.1 5,235,792 - Pf6N2E2_4503 0.68 -1.9 5,235,891 + Pf6N2E2_4503 0.79 +0.1 5,236,013 + -0.7 5,236,013 + -0.7 5,236,013 + +2.1 5,236,013 + -0.3 5,236,013 + +1.0 5,236,014 - -1.0 5,236,014 - -0.7 5,236,014 - +2.1 5,236,014 - +0.1 5,236,094 + +0.9 5,236,094 + -1.5 5,236,094 + +0.8 5,236,094 + -0.1 5,236,095 - +1.9 5,236,132 + -1.6 5,236,169 + -0.9 5,236,169 + -1.1 5,236,169 + +0.1 5,236,532 - Pf6N2E2_4504 0.36 -1.2 5,236,568 + Pf6N2E2_4504 0.39 -0.8 5,236,569 - Pf6N2E2_4504 0.39 -0.8 5,236,591 + Pf6N2E2_4504 0.41 -0.7 5,236,785 + Pf6N2E2_4504 0.58 -1.6 5,237,075 + Pf6N2E2_4504 0.83 -1.6 5,237,076 - Pf6N2E2_4504 0.83 +0.9
Or see this region's nucleotide sequence