Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4498 and Pf6N2E2_4499 are separated by 120 nucleotides Pf6N2E2_4499 and Pf6N2E2_4500 overlap by 11 nucleotides Pf6N2E2_4500 and Pf6N2E2_4501 are separated by 438 nucleotides
Pf6N2E2_4498 - Outer membrane porin, OprD family, at 5,230,894 to 5,232,216
_4498
Pf6N2E2_4499 - protein of unknown function DUF86, at 5,232,337 to 5,232,765
_4499
Pf6N2E2_4500 - hypothetical protein, at 5,232,755 to 5,232,916
_4500
Pf6N2E2_4501 - Alkyl hydroperoxide reductase subunit C-like protein, at 5,233,355 to 5,234,122
_4501
Position (kb)
5232
5233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5231.446 kb on - strand, within Pf6N2E2_4498 at 5231.527 kb on + strand, within Pf6N2E2_4498 at 5231.528 kb on - strand, within Pf6N2E2_4498 at 5231.528 kb on - strand, within Pf6N2E2_4498 at 5231.528 kb on - strand, within Pf6N2E2_4498 at 5231.528 kb on - strand, within Pf6N2E2_4498 at 5231.528 kb on - strand, within Pf6N2E2_4498 at 5231.528 kb on - strand, within Pf6N2E2_4498 at 5231.542 kb on + strand, within Pf6N2E2_4498 at 5231.542 kb on + strand, within Pf6N2E2_4498 at 5231.575 kb on + strand, within Pf6N2E2_4498 at 5231.575 kb on + strand, within Pf6N2E2_4498 at 5231.575 kb on + strand, within Pf6N2E2_4498 at 5231.575 kb on + strand, within Pf6N2E2_4498 at 5231.575 kb on + strand, within Pf6N2E2_4498 at 5231.575 kb on + strand, within Pf6N2E2_4498 at 5231.575 kb on + strand, within Pf6N2E2_4498 at 5231.576 kb on - strand, within Pf6N2E2_4498 at 5231.576 kb on - strand, within Pf6N2E2_4498 at 5231.576 kb on - strand, within Pf6N2E2_4498 at 5231.578 kb on + strand, within Pf6N2E2_4498 at 5231.578 kb on + strand, within Pf6N2E2_4498 at 5231.579 kb on - strand, within Pf6N2E2_4498 at 5231.579 kb on - strand, within Pf6N2E2_4498 at 5231.599 kb on + strand, within Pf6N2E2_4498 at 5231.599 kb on + strand, within Pf6N2E2_4498 at 5231.599 kb on + strand, within Pf6N2E2_4498 at 5231.599 kb on + strand, within Pf6N2E2_4498 at 5231.599 kb on + strand, within Pf6N2E2_4498 at 5231.599 kb on + strand, within Pf6N2E2_4498 at 5231.599 kb on + strand, within Pf6N2E2_4498 at 5231.600 kb on - strand, within Pf6N2E2_4498 at 5231.600 kb on - strand, within Pf6N2E2_4498 at 5231.600 kb on - strand, within Pf6N2E2_4498 at 5231.600 kb on - strand, within Pf6N2E2_4498 at 5231.600 kb on - strand, within Pf6N2E2_4498 at 5231.600 kb on - strand, within Pf6N2E2_4498 at 5231.651 kb on + strand, within Pf6N2E2_4498 at 5231.651 kb on + strand, within Pf6N2E2_4498 at 5231.651 kb on + strand, within Pf6N2E2_4498 at 5231.652 kb on - strand, within Pf6N2E2_4498 at 5231.671 kb on + strand, within Pf6N2E2_4498 at 5231.672 kb on - strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.716 kb on + strand, within Pf6N2E2_4498 at 5231.717 kb on - strand, within Pf6N2E2_4498 at 5231.717 kb on - strand, within Pf6N2E2_4498 at 5231.719 kb on + strand, within Pf6N2E2_4498 at 5231.719 kb on + strand, within Pf6N2E2_4498 at 5231.719 kb on + strand, within Pf6N2E2_4498 at 5231.719 kb on + strand, within Pf6N2E2_4498 at 5231.719 kb on + strand, within Pf6N2E2_4498 at 5231.719 kb on + strand, within Pf6N2E2_4498 at 5231.720 kb on - strand, within Pf6N2E2_4498 at 5231.720 kb on - strand, within Pf6N2E2_4498 at 5231.720 kb on - strand, within Pf6N2E2_4498 at 5231.720 kb on - strand, within Pf6N2E2_4498 at 5231.720 kb on - strand, within Pf6N2E2_4498 at 5231.720 kb on - strand, within Pf6N2E2_4498 at 5231.720 kb on - strand, within Pf6N2E2_4498 at 5231.731 kb on + strand, within Pf6N2E2_4498 at 5231.731 kb on + strand, within Pf6N2E2_4498 at 5231.777 kb on - strand, within Pf6N2E2_4498 at 5231.891 kb on - strand, within Pf6N2E2_4498 at 5231.891 kb on - strand, within Pf6N2E2_4498 at 5231.891 kb on - strand, within Pf6N2E2_4498 at 5231.940 kb on + strand, within Pf6N2E2_4498 at 5231.940 kb on + strand, within Pf6N2E2_4498 at 5231.940 kb on + strand, within Pf6N2E2_4498 at 5231.940 kb on + strand, within Pf6N2E2_4498 at 5231.940 kb on + strand, within Pf6N2E2_4498 at 5231.941 kb on - strand, within Pf6N2E2_4498 at 5231.941 kb on - strand, within Pf6N2E2_4498 at 5231.941 kb on - strand, within Pf6N2E2_4498 at 5231.941 kb on - strand, within Pf6N2E2_4498 at 5231.956 kb on + strand, within Pf6N2E2_4498 at 5231.962 kb on + strand, within Pf6N2E2_4498 at 5231.962 kb on + strand, within Pf6N2E2_4498 at 5231.963 kb on - strand, within Pf6N2E2_4498 at 5232.082 kb on + strand, within Pf6N2E2_4498 at 5232.082 kb on + strand, within Pf6N2E2_4498 at 5232.082 kb on + strand, within Pf6N2E2_4498 at 5232.083 kb on - strand, within Pf6N2E2_4498 at 5232.136 kb on - strand at 5232.196 kb on + strand at 5232.197 kb on - strand at 5232.197 kb on - strand at 5232.214 kb on + strand at 5232.215 kb on - strand at 5232.293 kb on + strand at 5232.293 kb on + strand at 5232.293 kb on + strand at 5232.294 kb on - strand at 5232.294 kb on - strand at 5232.294 kb on - strand at 5232.294 kb on - strand at 5232.303 kb on + strand at 5232.303 kb on + strand at 5232.310 kb on + strand at 5232.311 kb on - strand at 5232.311 kb on - strand at 5232.338 kb on + strand at 5232.338 kb on + strand at 5232.338 kb on + strand at 5232.339 kb on - strand at 5232.339 kb on - strand at 5232.339 kb on - strand at 5232.339 kb on - strand at 5232.602 kb on - strand, within Pf6N2E2_4499 at 5232.679 kb on - strand, within Pf6N2E2_4499 at 5232.696 kb on - strand, within Pf6N2E2_4499 at 5232.696 kb on - strand, within Pf6N2E2_4499 at 5232.752 kb on - strand at 5232.752 kb on - strand at 5232.752 kb on - strand at 5233.131 kb on - strand at 5233.131 kb on - strand at 5233.131 kb on - strand at 5233.134 kb on - strand at 5233.134 kb on - strand at 5233.134 kb on - strand at 5233.158 kb on - strand at 5233.158 kb on - strand at 5233.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 5,231,446 - Pf6N2E2_4498 0.42 +0.1 5,231,527 + Pf6N2E2_4498 0.48 -1.5 5,231,528 - Pf6N2E2_4498 0.48 -0.8 5,231,528 - Pf6N2E2_4498 0.48 -1.1 5,231,528 - Pf6N2E2_4498 0.48 +0.3 5,231,528 - Pf6N2E2_4498 0.48 -0.4 5,231,528 - Pf6N2E2_4498 0.48 -0.3 5,231,528 - Pf6N2E2_4498 0.48 +0.6 5,231,542 + Pf6N2E2_4498 0.49 +0.7 5,231,542 + Pf6N2E2_4498 0.49 +2.1 5,231,575 + Pf6N2E2_4498 0.51 +1.2 5,231,575 + Pf6N2E2_4498 0.51 -0.0 5,231,575 + Pf6N2E2_4498 0.51 +0.1 5,231,575 + Pf6N2E2_4498 0.51 +0.2 5,231,575 + Pf6N2E2_4498 0.51 -1.1 5,231,575 + Pf6N2E2_4498 0.51 +1.3 5,231,575 + Pf6N2E2_4498 0.51 +2.1 5,231,576 - Pf6N2E2_4498 0.52 -0.4 5,231,576 - Pf6N2E2_4498 0.52 +2.1 5,231,576 - Pf6N2E2_4498 0.52 -0.9 5,231,578 + Pf6N2E2_4498 0.52 +1.1 5,231,578 + Pf6N2E2_4498 0.52 +0.8 5,231,579 - Pf6N2E2_4498 0.52 -0.7 5,231,579 - Pf6N2E2_4498 0.52 -0.9 5,231,599 + Pf6N2E2_4498 0.53 +0.3 5,231,599 + Pf6N2E2_4498 0.53 +0.3 5,231,599 + Pf6N2E2_4498 0.53 +0.1 5,231,599 + Pf6N2E2_4498 0.53 -2.5 5,231,599 + Pf6N2E2_4498 0.53 +1.8 5,231,599 + Pf6N2E2_4498 0.53 -0.3 5,231,599 + Pf6N2E2_4498 0.53 -0.9 5,231,600 - Pf6N2E2_4498 0.53 -0.1 5,231,600 - Pf6N2E2_4498 0.53 -0.7 5,231,600 - Pf6N2E2_4498 0.53 +0.7 5,231,600 - Pf6N2E2_4498 0.53 -0.5 5,231,600 - Pf6N2E2_4498 0.53 +2.1 5,231,600 - Pf6N2E2_4498 0.53 +2.1 5,231,651 + Pf6N2E2_4498 0.57 -0.5 5,231,651 + Pf6N2E2_4498 0.57 +0.3 5,231,651 + Pf6N2E2_4498 0.57 -2.0 5,231,652 - Pf6N2E2_4498 0.57 +1.1 5,231,671 + Pf6N2E2_4498 0.59 -0.3 5,231,672 - Pf6N2E2_4498 0.59 +0.0 5,231,716 + Pf6N2E2_4498 0.62 -0.7 5,231,716 + Pf6N2E2_4498 0.62 +1.1 5,231,716 + Pf6N2E2_4498 0.62 +0.1 5,231,716 + Pf6N2E2_4498 0.62 -0.2 5,231,716 + Pf6N2E2_4498 0.62 +0.1 5,231,716 + Pf6N2E2_4498 0.62 +0.4 5,231,716 + Pf6N2E2_4498 0.62 +0.5 5,231,716 + Pf6N2E2_4498 0.62 +1.1 5,231,717 - Pf6N2E2_4498 0.62 +0.5 5,231,717 - Pf6N2E2_4498 0.62 -2.0 5,231,719 + Pf6N2E2_4498 0.62 +2.1 5,231,719 + Pf6N2E2_4498 0.62 +1.0 5,231,719 + Pf6N2E2_4498 0.62 +2.1 5,231,719 + Pf6N2E2_4498 0.62 +2.1 5,231,719 + Pf6N2E2_4498 0.62 -0.3 5,231,719 + Pf6N2E2_4498 0.62 -1.7 5,231,720 - Pf6N2E2_4498 0.62 +1.8 5,231,720 - Pf6N2E2_4498 0.62 -0.9 5,231,720 - Pf6N2E2_4498 0.62 +1.1 5,231,720 - Pf6N2E2_4498 0.62 -0.5 5,231,720 - Pf6N2E2_4498 0.62 +0.3 5,231,720 - Pf6N2E2_4498 0.62 -1.0 5,231,720 - Pf6N2E2_4498 0.62 +0.4 5,231,731 + Pf6N2E2_4498 0.63 -1.0 5,231,731 + Pf6N2E2_4498 0.63 +1.1 5,231,777 - Pf6N2E2_4498 0.67 +0.7 5,231,891 - Pf6N2E2_4498 0.75 -1.5 5,231,891 - Pf6N2E2_4498 0.75 +0.3 5,231,891 - Pf6N2E2_4498 0.75 -0.5 5,231,940 + Pf6N2E2_4498 0.79 -1.0 5,231,940 + Pf6N2E2_4498 0.79 -0.5 5,231,940 + Pf6N2E2_4498 0.79 +1.3 5,231,940 + Pf6N2E2_4498 0.79 +1.0 5,231,940 + Pf6N2E2_4498 0.79 +0.9 5,231,941 - Pf6N2E2_4498 0.79 -0.7 5,231,941 - Pf6N2E2_4498 0.79 -1.7 5,231,941 - Pf6N2E2_4498 0.79 -1.3 5,231,941 - Pf6N2E2_4498 0.79 +2.1 5,231,956 + Pf6N2E2_4498 0.80 -0.3 5,231,962 + Pf6N2E2_4498 0.81 -1.7 5,231,962 + Pf6N2E2_4498 0.81 +0.1 5,231,963 - Pf6N2E2_4498 0.81 +1.1 5,232,082 + Pf6N2E2_4498 0.90 -0.5 5,232,082 + Pf6N2E2_4498 0.90 -1.6 5,232,082 + Pf6N2E2_4498 0.90 -0.4 5,232,083 - Pf6N2E2_4498 0.90 +0.5 5,232,136 - -0.0 5,232,196 + +0.5 5,232,197 - +2.1 5,232,197 - +0.5 5,232,214 + -1.7 5,232,215 - -0.3 5,232,293 + +0.1 5,232,293 + -1.6 5,232,293 + -0.5 5,232,294 - -0.4 5,232,294 - -0.3 5,232,294 - -0.7 5,232,294 - -0.4 5,232,303 + +0.5 5,232,303 + -0.5 5,232,310 + +0.1 5,232,311 - +0.1 5,232,311 - +1.6 5,232,338 + -0.9 5,232,338 + -0.3 5,232,338 + -0.0 5,232,339 - +0.1 5,232,339 - +2.1 5,232,339 - -2.5 5,232,339 - -1.1 5,232,602 - Pf6N2E2_4499 0.62 -0.5 5,232,679 - Pf6N2E2_4499 0.80 -1.7 5,232,696 - Pf6N2E2_4499 0.84 +1.1 5,232,696 - Pf6N2E2_4499 0.84 +0.1 5,232,752 - +0.5 5,232,752 - -0.2 5,232,752 - -1.5 5,233,131 - +0.1 5,233,131 - -0.8 5,233,131 - -0.7 5,233,134 - +0.3 5,233,134 - -0.6 5,233,134 - -1.5 5,233,158 - -0.6 5,233,158 - +0.0 5,233,190 - -1.3
Or see this region's nucleotide sequence