Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3721 and Pf6N2E2_3722 overlap by 4 nucleotides Pf6N2E2_3722 and Pf6N2E2_3723 overlap by 14 nucleotides
Pf6N2E2_3721 - FIG021862: membrane protein, exporter, at 4,333,821 to 4,336,139
_3721
Pf6N2E2_3722 - FIG064705: cation/hydrogen antiporter, at 4,336,136 to 4,337,293
_3722
Pf6N2E2_3723 - FIG022199: FAD-binding protein, at 4,337,280 to 4,338,527
_3723
Position (kb) 4336
4337
4338 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4335.336 kb on + strand, within Pf6N2E2_3721 at 4335.336 kb on + strand, within Pf6N2E2_3721 at 4335.337 kb on - strand, within Pf6N2E2_3721 at 4335.478 kb on + strand, within Pf6N2E2_3721 at 4335.478 kb on + strand, within Pf6N2E2_3721 at 4335.479 kb on - strand, within Pf6N2E2_3721 at 4335.479 kb on - strand, within Pf6N2E2_3721 at 4335.479 kb on - strand, within Pf6N2E2_3721 at 4335.649 kb on + strand, within Pf6N2E2_3721 at 4335.650 kb on - strand, within Pf6N2E2_3721 at 4335.650 kb on - strand, within Pf6N2E2_3721 at 4335.909 kb on + strand at 4336.035 kb on - strand at 4336.035 kb on - strand at 4336.268 kb on + strand, within Pf6N2E2_3722 at 4336.269 kb on - strand, within Pf6N2E2_3722 at 4336.269 kb on - strand, within Pf6N2E2_3722 at 4336.269 kb on - strand, within Pf6N2E2_3722 at 4336.341 kb on - strand, within Pf6N2E2_3722 at 4336.553 kb on + strand, within Pf6N2E2_3722 at 4336.553 kb on + strand, within Pf6N2E2_3722 at 4336.553 kb on + strand, within Pf6N2E2_3722 at 4336.553 kb on + strand, within Pf6N2E2_3722 at 4336.553 kb on + strand, within Pf6N2E2_3722 at 4336.553 kb on + strand, within Pf6N2E2_3722 at 4336.553 kb on + strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.554 kb on - strand, within Pf6N2E2_3722 at 4336.560 kb on - strand, within Pf6N2E2_3722 at 4336.560 kb on - strand, within Pf6N2E2_3722 at 4336.754 kb on + strand, within Pf6N2E2_3722 at 4336.754 kb on + strand, within Pf6N2E2_3722 at 4336.754 kb on + strand, within Pf6N2E2_3722 at 4336.754 kb on + strand, within Pf6N2E2_3722 at 4336.755 kb on - strand, within Pf6N2E2_3722 at 4336.908 kb on - strand, within Pf6N2E2_3722 at 4336.908 kb on - strand, within Pf6N2E2_3722 at 4337.029 kb on - strand, within Pf6N2E2_3722 at 4337.029 kb on - strand, within Pf6N2E2_3722 at 4337.029 kb on - strand, within Pf6N2E2_3722 at 4337.098 kb on + strand, within Pf6N2E2_3722 at 4337.180 kb on + strand at 4337.180 kb on + strand at 4337.180 kb on + strand at 4337.180 kb on + strand at 4337.181 kb on - strand at 4337.181 kb on - strand at 4337.181 kb on - strand at 4337.181 kb on - strand at 4337.245 kb on + strand at 4337.245 kb on + strand at 4337.246 kb on - strand at 4337.246 kb on - strand at 4337.246 kb on - strand at 4337.246 kb on - strand at 4337.246 kb on - strand at 4337.246 kb on - strand at 4337.562 kb on - strand, within Pf6N2E2_3723 at 4337.739 kb on + strand, within Pf6N2E2_3723 at 4337.740 kb on - strand, within Pf6N2E2_3723
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 4,335,336 + Pf6N2E2_3721 0.65 +0.9 4,335,336 + Pf6N2E2_3721 0.65 -2.0 4,335,337 - Pf6N2E2_3721 0.65 +0.2 4,335,478 + Pf6N2E2_3721 0.71 +0.7 4,335,478 + Pf6N2E2_3721 0.71 -0.6 4,335,479 - Pf6N2E2_3721 0.71 +0.8 4,335,479 - Pf6N2E2_3721 0.71 +0.2 4,335,479 - Pf6N2E2_3721 0.71 +0.7 4,335,649 + Pf6N2E2_3721 0.79 +2.0 4,335,650 - Pf6N2E2_3721 0.79 +0.5 4,335,650 - Pf6N2E2_3721 0.79 -0.3 4,335,909 + +0.4 4,336,035 - -1.0 4,336,035 - +2.0 4,336,268 + Pf6N2E2_3722 0.11 -3.1 4,336,269 - Pf6N2E2_3722 0.11 +0.4 4,336,269 - Pf6N2E2_3722 0.11 -2.5 4,336,269 - Pf6N2E2_3722 0.11 +0.2 4,336,341 - Pf6N2E2_3722 0.18 +0.8 4,336,553 + Pf6N2E2_3722 0.36 +0.5 4,336,553 + Pf6N2E2_3722 0.36 +1.9 4,336,553 + Pf6N2E2_3722 0.36 +0.4 4,336,553 + Pf6N2E2_3722 0.36 -1.8 4,336,553 + Pf6N2E2_3722 0.36 -0.1 4,336,553 + Pf6N2E2_3722 0.36 -0.6 4,336,553 + Pf6N2E2_3722 0.36 -1.7 4,336,554 - Pf6N2E2_3722 0.36 +0.7 4,336,554 - Pf6N2E2_3722 0.36 +0.1 4,336,554 - Pf6N2E2_3722 0.36 +1.5 4,336,554 - Pf6N2E2_3722 0.36 +2.0 4,336,554 - Pf6N2E2_3722 0.36 +0.0 4,336,554 - Pf6N2E2_3722 0.36 +0.8 4,336,554 - Pf6N2E2_3722 0.36 -0.8 4,336,554 - Pf6N2E2_3722 0.36 +1.0 4,336,560 - Pf6N2E2_3722 0.37 +1.3 4,336,560 - Pf6N2E2_3722 0.37 +0.8 4,336,754 + Pf6N2E2_3722 0.53 +2.0 4,336,754 + Pf6N2E2_3722 0.53 -1.2 4,336,754 + Pf6N2E2_3722 0.53 +0.3 4,336,754 + Pf6N2E2_3722 0.53 +0.9 4,336,755 - Pf6N2E2_3722 0.53 +1.0 4,336,908 - Pf6N2E2_3722 0.67 +0.7 4,336,908 - Pf6N2E2_3722 0.67 +0.4 4,337,029 - Pf6N2E2_3722 0.77 -0.4 4,337,029 - Pf6N2E2_3722 0.77 -1.6 4,337,029 - Pf6N2E2_3722 0.77 +1.2 4,337,098 + Pf6N2E2_3722 0.83 +0.1 4,337,180 + +3.0 4,337,180 + +1.0 4,337,180 + +0.4 4,337,180 + -0.6 4,337,181 - +0.4 4,337,181 - -1.2 4,337,181 - +0.6 4,337,181 - -1.3 4,337,245 + -0.7 4,337,245 + +0.2 4,337,246 - -0.6 4,337,246 - +0.6 4,337,246 - +1.5 4,337,246 - +0.4 4,337,246 - +1.0 4,337,246 - +0.1 4,337,562 - Pf6N2E2_3723 0.23 +0.0 4,337,739 + Pf6N2E2_3723 0.37 -0.4 4,337,740 - Pf6N2E2_3723 0.37 -0.3
Or see this region's nucleotide sequence