Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2707 and Pf6N2E2_2708 are separated by 8 nucleotides Pf6N2E2_2708 and Pf6N2E2_2709 are separated by 64 nucleotides Pf6N2E2_2709 and Pf6N2E2_2710 are separated by 546 nucleotides
Pf6N2E2_2707 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,200,157 to 3,200,849
_2707
Pf6N2E2_2708 - Lactam utilization protein LamB, at 3,200,858 to 3,201,610
_2708
Pf6N2E2_2709 - Sialic acid transporter (permease) NanT, at 3,201,675 to 3,202,961
_2709
Pf6N2E2_2710 - FIG00953701: hypothetical protein, at 3,203,508 to 3,204,050
_2710
Position (kb)
3201
3202
3203 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.709 kb on - strand, within Pf6N2E2_2707 at 3200.710 kb on + strand, within Pf6N2E2_2707 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3201.090 kb on + strand, within Pf6N2E2_2708 at 3201.091 kb on - strand, within Pf6N2E2_2708 at 3201.204 kb on + strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.288 kb on + strand, within Pf6N2E2_2708 at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.643 kb on - strand at 3201.643 kb on - strand at 3201.676 kb on + strand at 3201.676 kb on + strand at 3201.743 kb on - strand at 3201.743 kb on - strand at 3201.847 kb on + strand, within Pf6N2E2_2709 at 3201.882 kb on - strand, within Pf6N2E2_2709 at 3201.971 kb on + strand, within Pf6N2E2_2709 at 3201.993 kb on - strand, within Pf6N2E2_2709 at 3201.994 kb on + strand, within Pf6N2E2_2709 at 3201.994 kb on + strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3201.995 kb on - strand, within Pf6N2E2_2709 at 3202.069 kb on + strand, within Pf6N2E2_2709 at 3202.069 kb on + strand, within Pf6N2E2_2709 at 3202.069 kb on + strand, within Pf6N2E2_2709 at 3202.070 kb on - strand, within Pf6N2E2_2709 at 3202.070 kb on - strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.084 kb on + strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.085 kb on - strand, within Pf6N2E2_2709 at 3202.093 kb on + strand, within Pf6N2E2_2709 at 3202.093 kb on + strand, within Pf6N2E2_2709 at 3202.093 kb on + strand, within Pf6N2E2_2709 at 3202.094 kb on - strand, within Pf6N2E2_2709 at 3202.094 kb on - strand, within Pf6N2E2_2709 at 3202.094 kb on - strand, within Pf6N2E2_2709 at 3202.121 kb on - strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.137 kb on + strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.138 kb on - strand, within Pf6N2E2_2709 at 3202.192 kb on + strand, within Pf6N2E2_2709 at 3202.192 kb on + strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.193 kb on - strand, within Pf6N2E2_2709 at 3202.270 kb on + strand, within Pf6N2E2_2709 at 3202.270 kb on + strand, within Pf6N2E2_2709 at 3202.317 kb on - strand, within Pf6N2E2_2709 at 3202.318 kb on + strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.319 kb on - strand, within Pf6N2E2_2709 at 3202.439 kb on - strand, within Pf6N2E2_2709 at 3202.439 kb on - strand, within Pf6N2E2_2709 at 3202.439 kb on - strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.606 kb on + strand, within Pf6N2E2_2709 at 3202.607 kb on - strand, within Pf6N2E2_2709 at 3202.607 kb on - strand, within Pf6N2E2_2709 at 3202.739 kb on + strand, within Pf6N2E2_2709 at 3202.739 kb on + strand, within Pf6N2E2_2709 at 3202.740 kb on - strand, within Pf6N2E2_2709 at 3202.740 kb on - strand, within Pf6N2E2_2709 at 3202.772 kb on + strand, within Pf6N2E2_2709 at 3202.772 kb on + strand, within Pf6N2E2_2709 at 3202.773 kb on - strand, within Pf6N2E2_2709 at 3202.980 kb on + strand at 3202.980 kb on + strand at 3202.981 kb on - strand at 3202.988 kb on + strand at 3202.988 kb on + strand at 3202.988 kb on + strand at 3202.988 kb on + strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3202.989 kb on - strand at 3203.060 kb on + strand at 3203.079 kb on + strand at 3203.080 kb on - strand at 3203.133 kb on + strand at 3203.200 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.207 kb on + strand at 3203.208 kb on - strand at 3203.208 kb on - strand at 3203.208 kb on - strand at 3203.208 kb on - strand at 3203.328 kb on + strand at 3203.329 kb on - strand at 3203.406 kb on - strand at 3203.674 kb on + strand, within Pf6N2E2_2710 at 3203.675 kb on - strand, within Pf6N2E2_2710 at 3203.675 kb on - strand, within Pf6N2E2_2710 at 3203.675 kb on - strand, within Pf6N2E2_2710 at 3203.681 kb on - strand, within Pf6N2E2_2710 at 3203.747 kb on - strand, within Pf6N2E2_2710
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 3,200,690 - Pf6N2E2_2707 0.77 -0.0 3,200,690 - Pf6N2E2_2707 0.77 -2.5 3,200,709 - Pf6N2E2_2707 0.80 -0.5 3,200,710 + Pf6N2E2_2707 0.80 +0.4 3,200,954 - Pf6N2E2_2708 0.13 -3.5 3,200,954 - Pf6N2E2_2708 0.13 -0.9 3,200,954 - Pf6N2E2_2708 0.13 -2.5 3,201,090 + Pf6N2E2_2708 0.31 +2.0 3,201,091 - Pf6N2E2_2708 0.31 +2.0 3,201,204 + Pf6N2E2_2708 0.46 +2.0 3,201,265 - Pf6N2E2_2708 0.54 -2.2 3,201,265 - Pf6N2E2_2708 0.54 -1.9 3,201,265 - Pf6N2E2_2708 0.54 -1.0 3,201,265 - Pf6N2E2_2708 0.54 -0.8 3,201,265 - Pf6N2E2_2708 0.54 +0.4 3,201,288 + Pf6N2E2_2708 0.57 -1.4 3,201,626 - -0.9 3,201,626 - -0.8 3,201,626 - -0.6 3,201,626 - +0.4 3,201,643 - -0.1 3,201,643 - -0.9 3,201,676 + +0.3 3,201,676 + +0.9 3,201,743 - -0.7 3,201,743 - -2.1 3,201,847 + Pf6N2E2_2709 0.13 -1.4 3,201,882 - Pf6N2E2_2709 0.16 -0.0 3,201,971 + Pf6N2E2_2709 0.23 -0.4 3,201,993 - Pf6N2E2_2709 0.25 +0.7 3,201,994 + Pf6N2E2_2709 0.25 +1.0 3,201,994 + Pf6N2E2_2709 0.25 +0.5 3,201,995 - Pf6N2E2_2709 0.25 +0.7 3,201,995 - Pf6N2E2_2709 0.25 +2.0 3,201,995 - Pf6N2E2_2709 0.25 -1.2 3,201,995 - Pf6N2E2_2709 0.25 +0.1 3,201,995 - Pf6N2E2_2709 0.25 -0.6 3,201,995 - Pf6N2E2_2709 0.25 +0.0 3,201,995 - Pf6N2E2_2709 0.25 +0.8 3,201,995 - Pf6N2E2_2709 0.25 -0.9 3,202,069 + Pf6N2E2_2709 0.31 -1.6 3,202,069 + Pf6N2E2_2709 0.31 +1.0 3,202,069 + Pf6N2E2_2709 0.31 -0.8 3,202,070 - Pf6N2E2_2709 0.31 -0.0 3,202,070 - Pf6N2E2_2709 0.31 -0.7 3,202,084 + Pf6N2E2_2709 0.32 +0.4 3,202,084 + Pf6N2E2_2709 0.32 -0.3 3,202,084 + Pf6N2E2_2709 0.32 +2.0 3,202,084 + Pf6N2E2_2709 0.32 +0.5 3,202,085 - Pf6N2E2_2709 0.32 -0.6 3,202,085 - Pf6N2E2_2709 0.32 -0.0 3,202,085 - Pf6N2E2_2709 0.32 -0.2 3,202,085 - Pf6N2E2_2709 0.32 +0.1 3,202,093 + Pf6N2E2_2709 0.32 -0.5 3,202,093 + Pf6N2E2_2709 0.32 -2.2 3,202,093 + Pf6N2E2_2709 0.32 -1.3 3,202,094 - Pf6N2E2_2709 0.33 -2.4 3,202,094 - Pf6N2E2_2709 0.33 +0.7 3,202,094 - Pf6N2E2_2709 0.33 +0.9 3,202,121 - Pf6N2E2_2709 0.35 -0.4 3,202,137 + Pf6N2E2_2709 0.36 +0.5 3,202,137 + Pf6N2E2_2709 0.36 -0.9 3,202,137 + Pf6N2E2_2709 0.36 +1.0 3,202,137 + Pf6N2E2_2709 0.36 +1.0 3,202,137 + Pf6N2E2_2709 0.36 -0.7 3,202,137 + Pf6N2E2_2709 0.36 +0.2 3,202,137 + Pf6N2E2_2709 0.36 -0.3 3,202,137 + Pf6N2E2_2709 0.36 -1.1 3,202,137 + Pf6N2E2_2709 0.36 -0.7 3,202,137 + Pf6N2E2_2709 0.36 +2.0 3,202,138 - Pf6N2E2_2709 0.36 +0.8 3,202,138 - Pf6N2E2_2709 0.36 -0.9 3,202,138 - Pf6N2E2_2709 0.36 -0.9 3,202,138 - Pf6N2E2_2709 0.36 -1.7 3,202,138 - Pf6N2E2_2709 0.36 -0.3 3,202,138 - Pf6N2E2_2709 0.36 -0.3 3,202,138 - Pf6N2E2_2709 0.36 -0.2 3,202,138 - Pf6N2E2_2709 0.36 -0.0 3,202,138 - Pf6N2E2_2709 0.36 -0.2 3,202,138 - Pf6N2E2_2709 0.36 -2.1 3,202,192 + Pf6N2E2_2709 0.40 -0.0 3,202,192 + Pf6N2E2_2709 0.40 -0.6 3,202,193 - Pf6N2E2_2709 0.40 +2.0 3,202,193 - Pf6N2E2_2709 0.40 +2.0 3,202,193 - Pf6N2E2_2709 0.40 -3.0 3,202,193 - Pf6N2E2_2709 0.40 -1.3 3,202,270 + Pf6N2E2_2709 0.46 +2.0 3,202,270 + Pf6N2E2_2709 0.46 -0.8 3,202,317 - Pf6N2E2_2709 0.50 -1.3 3,202,318 + Pf6N2E2_2709 0.50 -1.2 3,202,319 - Pf6N2E2_2709 0.50 -0.7 3,202,319 - Pf6N2E2_2709 0.50 +2.0 3,202,319 - Pf6N2E2_2709 0.50 +0.8 3,202,319 - Pf6N2E2_2709 0.50 -0.0 3,202,439 - Pf6N2E2_2709 0.59 -0.8 3,202,439 - Pf6N2E2_2709 0.59 -1.5 3,202,439 - Pf6N2E2_2709 0.59 -1.2 3,202,606 + Pf6N2E2_2709 0.72 +2.0 3,202,606 + Pf6N2E2_2709 0.72 +0.1 3,202,606 + Pf6N2E2_2709 0.72 -0.0 3,202,606 + Pf6N2E2_2709 0.72 +2.0 3,202,606 + Pf6N2E2_2709 0.72 +0.8 3,202,607 - Pf6N2E2_2709 0.72 +1.0 3,202,607 - Pf6N2E2_2709 0.72 -1.2 3,202,739 + Pf6N2E2_2709 0.83 +0.1 3,202,739 + Pf6N2E2_2709 0.83 -0.5 3,202,740 - Pf6N2E2_2709 0.83 +0.4 3,202,740 - Pf6N2E2_2709 0.83 -0.8 3,202,772 + Pf6N2E2_2709 0.85 -1.7 3,202,772 + Pf6N2E2_2709 0.85 +0.0 3,202,773 - Pf6N2E2_2709 0.85 -0.8 3,202,980 + -2.6 3,202,980 + +0.0 3,202,981 - +1.0 3,202,988 + +1.3 3,202,988 + -0.8 3,202,988 + +0.1 3,202,988 + +0.1 3,202,989 - +0.1 3,202,989 - -3.3 3,202,989 - -1.6 3,202,989 - -0.0 3,202,989 - +2.0 3,202,989 - +0.1 3,202,989 - -0.9 3,202,989 - -0.6 3,202,989 - -1.1 3,203,060 + -1.7 3,203,079 + -0.9 3,203,080 - -0.8 3,203,133 + +1.0 3,203,200 + -1.0 3,203,207 + +0.4 3,203,207 + +0.2 3,203,207 + -1.1 3,203,207 + -0.5 3,203,207 + +0.1 3,203,207 + -0.5 3,203,208 - -1.4 3,203,208 - +2.0 3,203,208 - +0.0 3,203,208 - -0.6 3,203,328 + -0.4 3,203,329 - +0.3 3,203,406 - +2.0 3,203,674 + Pf6N2E2_2710 0.31 -0.3 3,203,675 - Pf6N2E2_2710 0.31 +0.9 3,203,675 - Pf6N2E2_2710 0.31 +0.4 3,203,675 - Pf6N2E2_2710 0.31 -0.3 3,203,681 - Pf6N2E2_2710 0.32 -0.7 3,203,747 - Pf6N2E2_2710 0.44 -0.3
Or see this region's nucleotide sequence