Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_22 and Pf6N2E2_23 are separated by 275 nucleotides Pf6N2E2_23 and Pf6N2E2_24 are separated by 127 nucleotides Pf6N2E2_24 and Pf6N2E2_25 are separated by 75 nucleotides
Pf6N2E2_22 - L-ornithine 5-monooxygenase (EC 1.13.12.-), PvdA of pyoverdin biosynthesis, at 56,762 to 58,096
_22
Pf6N2E2_23 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 58,372 to 61,572
_23
Pf6N2E2_24 - hypothetical protein, at 61,700 to 62,134
_24
Pf6N2E2_25 - DNA-binding response regulator, LuxR family, at 62,210 to 62,863
_25
Position (kb) 58
59
60
61
62 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 57.614 kb on + strand, within Pf6N2E2_22 at 57.614 kb on + strand, within Pf6N2E2_22 at 57.614 kb on + strand, within Pf6N2E2_22 at 57.615 kb on - strand, within Pf6N2E2_22 at 57.615 kb on - strand, within Pf6N2E2_22 at 57.615 kb on - strand, within Pf6N2E2_22 at 57.650 kb on + strand, within Pf6N2E2_22 at 57.712 kb on + strand, within Pf6N2E2_22 at 57.712 kb on + strand, within Pf6N2E2_22 at 57.713 kb on - strand, within Pf6N2E2_22 at 57.899 kb on + strand, within Pf6N2E2_22 at 57.899 kb on + strand, within Pf6N2E2_22 at 57.899 kb on + strand, within Pf6N2E2_22 at 57.899 kb on + strand, within Pf6N2E2_22 at 57.900 kb on - strand, within Pf6N2E2_22 at 57.938 kb on + strand, within Pf6N2E2_22 at 57.938 kb on + strand, within Pf6N2E2_22 at 57.938 kb on + strand, within Pf6N2E2_22 at 57.939 kb on - strand, within Pf6N2E2_22 at 57.939 kb on - strand, within Pf6N2E2_22 at 57.939 kb on - strand, within Pf6N2E2_22 at 57.939 kb on - strand, within Pf6N2E2_22 at 57.939 kb on - strand, within Pf6N2E2_22 at 57.939 kb on - strand, within Pf6N2E2_22 at 57.939 kb on - strand, within Pf6N2E2_22 at 57.980 kb on - strand at 58.031 kb on + strand at 58.031 kb on + strand at 58.031 kb on + strand at 58.032 kb on - strand at 58.032 kb on - strand at 58.092 kb on + strand at 58.093 kb on - strand at 58.094 kb on + strand at 58.094 kb on + strand at 58.095 kb on - strand at 58.095 kb on - strand at 58.129 kb on + strand at 58.129 kb on + strand at 58.130 kb on - strand at 58.222 kb on + strand at 58.277 kb on + strand at 58.277 kb on + strand at 58.277 kb on + strand at 58.317 kb on + strand at 58.317 kb on + strand at 58.317 kb on + strand at 58.317 kb on + strand at 58.317 kb on + strand at 58.317 kb on + strand at 58.317 kb on + strand at 58.318 kb on - strand at 58.318 kb on - strand at 58.318 kb on - strand at 58.318 kb on - strand at 58.318 kb on - strand at 58.492 kb on + strand at 58.610 kb on - strand at 58.610 kb on - strand at 58.630 kb on + strand at 58.818 kb on - strand, within Pf6N2E2_23 at 58.883 kb on - strand, within Pf6N2E2_23 at 58.883 kb on - strand, within Pf6N2E2_23 at 58.883 kb on - strand, within Pf6N2E2_23 at 58.888 kb on + strand, within Pf6N2E2_23 at 58.889 kb on - strand, within Pf6N2E2_23 at 58.889 kb on - strand, within Pf6N2E2_23 at 58.997 kb on - strand, within Pf6N2E2_23 at 59.023 kb on + strand, within Pf6N2E2_23 at 59.024 kb on - strand, within Pf6N2E2_23 at 59.041 kb on + strand, within Pf6N2E2_23 at 59.052 kb on + strand, within Pf6N2E2_23 at 59.052 kb on + strand, within Pf6N2E2_23 at 59.053 kb on - strand, within Pf6N2E2_23 at 59.405 kb on + strand, within Pf6N2E2_23 at 59.406 kb on - strand, within Pf6N2E2_23 at 59.406 kb on - strand, within Pf6N2E2_23 at 59.758 kb on + strand, within Pf6N2E2_23 at 59.759 kb on - strand, within Pf6N2E2_23 at 59.759 kb on - strand, within Pf6N2E2_23 at 59.864 kb on - strand, within Pf6N2E2_23 at 59.959 kb on + strand, within Pf6N2E2_23 at 59.959 kb on + strand, within Pf6N2E2_23 at 59.959 kb on + strand, within Pf6N2E2_23 at 59.960 kb on - strand, within Pf6N2E2_23 at 59.960 kb on - strand, within Pf6N2E2_23 at 60.014 kb on + strand, within Pf6N2E2_23 at 60.015 kb on - strand, within Pf6N2E2_23 at 60.043 kb on + strand, within Pf6N2E2_23 at 60.044 kb on - strand, within Pf6N2E2_23 at 60.044 kb on - strand, within Pf6N2E2_23 at 60.044 kb on - strand, within Pf6N2E2_23 at 60.642 kb on + strand, within Pf6N2E2_23 at 60.642 kb on + strand, within Pf6N2E2_23 at 60.642 kb on + strand, within Pf6N2E2_23 at 60.783 kb on - strand, within Pf6N2E2_23 at 60.864 kb on + strand, within Pf6N2E2_23 at 61.498 kb on - strand at 61.498 kb on - strand at 61.570 kb on + strand at 61.570 kb on + strand at 61.571 kb on - strand at 61.571 kb on - strand at 61.571 kb on - strand at 61.571 kb on - strand at 61.603 kb on + strand at 61.609 kb on + strand at 61.609 kb on + strand at 61.610 kb on - strand at 61.610 kb on - strand at 61.649 kb on + strand at 61.650 kb on - strand at 61.650 kb on - strand at 61.684 kb on + strand at 61.685 kb on - strand at 61.787 kb on + strand, within Pf6N2E2_24 at 61.787 kb on + strand, within Pf6N2E2_24 at 61.788 kb on - strand, within Pf6N2E2_24 at 61.788 kb on - strand, within Pf6N2E2_24 at 61.842 kb on - strand, within Pf6N2E2_24 at 61.842 kb on - strand, within Pf6N2E2_24 at 61.918 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.952 kb on + strand, within Pf6N2E2_24 at 61.952 kb on + strand, within Pf6N2E2_24 at 61.953 kb on - strand, within Pf6N2E2_24 at 62.229 kb on + strand at 62.229 kb on + strand at 62.229 kb on + strand at 62.230 kb on - strand at 62.230 kb on - strand at 62.287 kb on - strand, within Pf6N2E2_25 at 62.287 kb on - strand, within Pf6N2E2_25 at 62.305 kb on + strand, within Pf6N2E2_25 at 62.305 kb on + strand, within Pf6N2E2_25 at 62.305 kb on + strand, within Pf6N2E2_25 at 62.306 kb on - strand, within Pf6N2E2_25 at 62.306 kb on - strand, within Pf6N2E2_25
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 57,614 + Pf6N2E2_22 0.64 +3.0 57,614 + Pf6N2E2_22 0.64 +1.0 57,614 + Pf6N2E2_22 0.64 -1.1 57,615 - Pf6N2E2_22 0.64 +2.0 57,615 - Pf6N2E2_22 0.64 +1.2 57,615 - Pf6N2E2_22 0.64 +0.4 57,650 + Pf6N2E2_22 0.67 -1.3 57,712 + Pf6N2E2_22 0.71 +1.0 57,712 + Pf6N2E2_22 0.71 +0.4 57,713 - Pf6N2E2_22 0.71 +0.9 57,899 + Pf6N2E2_22 0.85 -0.2 57,899 + Pf6N2E2_22 0.85 +0.2 57,899 + Pf6N2E2_22 0.85 -0.0 57,899 + Pf6N2E2_22 0.85 +1.0 57,900 - Pf6N2E2_22 0.85 -3.8 57,938 + Pf6N2E2_22 0.88 -0.7 57,938 + Pf6N2E2_22 0.88 +1.4 57,938 + Pf6N2E2_22 0.88 -1.1 57,939 - Pf6N2E2_22 0.88 -0.2 57,939 - Pf6N2E2_22 0.88 +1.2 57,939 - Pf6N2E2_22 0.88 +2.0 57,939 - Pf6N2E2_22 0.88 +2.0 57,939 - Pf6N2E2_22 0.88 +0.4 57,939 - Pf6N2E2_22 0.88 +1.5 57,939 - Pf6N2E2_22 0.88 +3.6 57,980 - +2.8 58,031 + +0.0 58,031 + +0.1 58,031 + -0.2 58,032 - +0.0 58,032 - +1.7 58,092 + +0.8 58,093 - +0.1 58,094 + +1.4 58,094 + -0.0 58,095 - +0.4 58,095 - +0.0 58,129 + +0.3 58,129 + -0.9 58,130 - -0.3 58,222 + -0.6 58,277 + -1.1 58,277 + -2.5 58,277 + -0.3 58,317 + -0.2 58,317 + -0.4 58,317 + +0.4 58,317 + -0.4 58,317 + -1.0 58,317 + +0.4 58,317 + +0.1 58,318 - +0.6 58,318 - +0.0 58,318 - +0.3 58,318 - +0.5 58,318 - +0.2 58,492 + +2.0 58,610 - +0.1 58,610 - +0.0 58,630 + +0.4 58,818 - Pf6N2E2_23 0.14 +0.7 58,883 - Pf6N2E2_23 0.16 -0.2 58,883 - Pf6N2E2_23 0.16 -0.9 58,883 - Pf6N2E2_23 0.16 -0.6 58,888 + Pf6N2E2_23 0.16 +2.0 58,889 - Pf6N2E2_23 0.16 -0.6 58,889 - Pf6N2E2_23 0.16 +0.5 58,997 - Pf6N2E2_23 0.20 +1.0 59,023 + Pf6N2E2_23 0.20 -1.4 59,024 - Pf6N2E2_23 0.20 +0.3 59,041 + Pf6N2E2_23 0.21 +2.0 59,052 + Pf6N2E2_23 0.21 +0.7 59,052 + Pf6N2E2_23 0.21 +0.4 59,053 - Pf6N2E2_23 0.21 +0.4 59,405 + Pf6N2E2_23 0.32 -0.5 59,406 - Pf6N2E2_23 0.32 -1.7 59,406 - Pf6N2E2_23 0.32 +0.0 59,758 + Pf6N2E2_23 0.43 +0.2 59,759 - Pf6N2E2_23 0.43 -0.5 59,759 - Pf6N2E2_23 0.43 -0.6 59,864 - Pf6N2E2_23 0.47 +0.0 59,959 + Pf6N2E2_23 0.50 +0.2 59,959 + Pf6N2E2_23 0.50 +0.3 59,959 + Pf6N2E2_23 0.50 +0.6 59,960 - Pf6N2E2_23 0.50 +1.8 59,960 - Pf6N2E2_23 0.50 +0.0 60,014 + Pf6N2E2_23 0.51 +0.0 60,015 - Pf6N2E2_23 0.51 -1.5 60,043 + Pf6N2E2_23 0.52 +1.7 60,044 - Pf6N2E2_23 0.52 +2.0 60,044 - Pf6N2E2_23 0.52 +2.0 60,044 - Pf6N2E2_23 0.52 +1.3 60,642 + Pf6N2E2_23 0.71 -0.5 60,642 + Pf6N2E2_23 0.71 -0.0 60,642 + Pf6N2E2_23 0.71 +2.0 60,783 - Pf6N2E2_23 0.75 +0.4 60,864 + Pf6N2E2_23 0.78 +1.3 61,498 - +2.0 61,498 - -0.3 61,570 + -1.3 61,570 + +2.0 61,571 - +0.0 61,571 - -0.8 61,571 - +0.4 61,571 - +2.0 61,603 + -0.6 61,609 + -1.0 61,609 + +2.0 61,610 - +1.0 61,610 - +1.0 61,649 + +1.9 61,650 - +2.0 61,650 - -0.2 61,684 + +1.4 61,685 - +0.9 61,787 + Pf6N2E2_24 0.20 +0.4 61,787 + Pf6N2E2_24 0.20 +2.0 61,788 - Pf6N2E2_24 0.20 -0.2 61,788 - Pf6N2E2_24 0.20 -0.6 61,842 - Pf6N2E2_24 0.33 +2.0 61,842 - Pf6N2E2_24 0.33 -1.1 61,918 + Pf6N2E2_24 0.50 -1.8 61,922 + Pf6N2E2_24 0.51 +0.0 61,922 + Pf6N2E2_24 0.51 +0.6 61,922 + Pf6N2E2_24 0.51 +0.1 61,922 + Pf6N2E2_24 0.51 +1.5 61,923 - Pf6N2E2_24 0.51 +0.7 61,923 - Pf6N2E2_24 0.51 +0.4 61,923 - Pf6N2E2_24 0.51 +0.0 61,923 - Pf6N2E2_24 0.51 +2.0 61,923 - Pf6N2E2_24 0.51 -0.2 61,923 - Pf6N2E2_24 0.51 +0.5 61,923 - Pf6N2E2_24 0.51 +2.0 61,952 + Pf6N2E2_24 0.58 -0.4 61,952 + Pf6N2E2_24 0.58 -0.5 61,953 - Pf6N2E2_24 0.58 -0.3 62,229 + -0.9 62,229 + -0.0 62,229 + +1.2 62,230 - -0.3 62,230 - +2.0 62,287 - Pf6N2E2_25 0.12 -0.2 62,287 - Pf6N2E2_25 0.12 +2.0 62,305 + Pf6N2E2_25 0.15 +0.3 62,305 + Pf6N2E2_25 0.15 +1.0 62,305 + Pf6N2E2_25 0.15 -0.1 62,306 - Pf6N2E2_25 0.15 +0.9 62,306 - Pf6N2E2_25 0.15 -0.6
Or see this region's nucleotide sequence