Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2042 and Pf6N2E2_2043 are separated by 43 nucleotides Pf6N2E2_2043 and Pf6N2E2_2044 are separated by 60 nucleotides
Pf6N2E2_2042 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 2,431,553 to 2,433,508
_2042
Pf6N2E2_2043 - Twitching motility protein PilT, at 2,433,552 to 2,434,739
_2043
Pf6N2E2_2044 - Histidine kinase/response regulator hybrid protein, at 2,434,800 to 2,437,337
_2044
Position (kb)
2433
2434
2435 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2432.559 kb on - strand, within Pf6N2E2_2042 at 2432.559 kb on - strand, within Pf6N2E2_2042 at 2432.749 kb on + strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.766 kb on - strand, within Pf6N2E2_2042 at 2433.029 kb on + strand, within Pf6N2E2_2042 at 2433.030 kb on - strand, within Pf6N2E2_2042 at 2433.030 kb on - strand, within Pf6N2E2_2042 at 2433.069 kb on - strand, within Pf6N2E2_2042 at 2433.367 kb on + strand at 2433.367 kb on + strand at 2433.368 kb on - strand at 2433.560 kb on + strand at 2433.614 kb on + strand at 2434.176 kb on - strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.320 kb on + strand, within Pf6N2E2_2043 at 2434.605 kb on - strand, within Pf6N2E2_2043 at 2434.605 kb on - strand, within Pf6N2E2_2043 at 2434.605 kb on - strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.741 kb on + strand at 2434.742 kb on - strand at 2434.742 kb on - strand at 2434.757 kb on + strand at 2434.757 kb on + strand at 2434.758 kb on - strand at 2434.760 kb on + strand at 2434.761 kb on - strand at 2434.761 kb on - strand at 2434.826 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.977 kb on + strand at 2435.042 kb on + strand at 2435.043 kb on - strand at 2435.043 kb on - strand at 2435.066 kb on + strand, within Pf6N2E2_2044 at 2435.066 kb on + strand, within Pf6N2E2_2044 at 2435.082 kb on + strand, within Pf6N2E2_2044 at 2435.082 kb on + strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.138 kb on - strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.182 kb on - strand, within Pf6N2E2_2044 at 2435.394 kb on - strand, within Pf6N2E2_2044 at 2435.457 kb on - strand, within Pf6N2E2_2044 at 2435.457 kb on - strand, within Pf6N2E2_2044 at 2435.556 kb on + strand, within Pf6N2E2_2044 at 2435.595 kb on + strand, within Pf6N2E2_2044 at 2435.595 kb on + strand, within Pf6N2E2_2044 at 2435.595 kb on + strand, within Pf6N2E2_2044 at 2435.596 kb on - strand, within Pf6N2E2_2044 at 2435.613 kb on + strand, within Pf6N2E2_2044 at 2435.613 kb on + strand, within Pf6N2E2_2044 at 2435.613 kb on + strand, within Pf6N2E2_2044 at 2435.614 kb on - strand, within Pf6N2E2_2044
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 2,432,559 - Pf6N2E2_2042 0.51 -0.3 2,432,559 - Pf6N2E2_2042 0.51 +0.3 2,432,749 + Pf6N2E2_2042 0.61 +0.3 2,432,750 - Pf6N2E2_2042 0.61 +2.0 2,432,750 - Pf6N2E2_2042 0.61 +1.0 2,432,750 - Pf6N2E2_2042 0.61 -1.0 2,432,753 + Pf6N2E2_2042 0.61 -0.6 2,432,753 + Pf6N2E2_2042 0.61 -1.1 2,432,753 + Pf6N2E2_2042 0.61 +0.4 2,432,753 + Pf6N2E2_2042 0.61 -1.5 2,432,753 + Pf6N2E2_2042 0.61 +0.5 2,432,753 + Pf6N2E2_2042 0.61 +0.5 2,432,754 - Pf6N2E2_2042 0.61 +0.6 2,432,754 - Pf6N2E2_2042 0.61 -0.3 2,432,754 - Pf6N2E2_2042 0.61 -0.2 2,432,754 - Pf6N2E2_2042 0.61 +0.0 2,432,754 - Pf6N2E2_2042 0.61 -0.0 2,432,754 - Pf6N2E2_2042 0.61 +0.2 2,432,759 + Pf6N2E2_2042 0.62 -0.4 2,432,759 + Pf6N2E2_2042 0.62 -0.1 2,432,759 + Pf6N2E2_2042 0.62 -1.0 2,432,759 + Pf6N2E2_2042 0.62 +2.0 2,432,759 + Pf6N2E2_2042 0.62 -0.1 2,432,759 + Pf6N2E2_2042 0.62 -0.8 2,432,760 - Pf6N2E2_2042 0.62 -1.5 2,432,760 - Pf6N2E2_2042 0.62 -0.2 2,432,760 - Pf6N2E2_2042 0.62 +0.2 2,432,760 - Pf6N2E2_2042 0.62 -0.5 2,432,760 - Pf6N2E2_2042 0.62 -0.8 2,432,766 - Pf6N2E2_2042 0.62 -0.2 2,433,029 + Pf6N2E2_2042 0.75 +0.5 2,433,030 - Pf6N2E2_2042 0.76 +2.0 2,433,030 - Pf6N2E2_2042 0.76 -0.6 2,433,069 - Pf6N2E2_2042 0.78 -1.2 2,433,367 + -0.3 2,433,367 + -0.7 2,433,368 - -0.3 2,433,560 + -0.4 2,433,614 + +0.6 2,434,176 - Pf6N2E2_2043 0.53 +1.0 2,434,177 + Pf6N2E2_2043 0.53 +1.0 2,434,177 + Pf6N2E2_2043 0.53 +2.0 2,434,177 + Pf6N2E2_2043 0.53 +1.0 2,434,177 + Pf6N2E2_2043 0.53 +1.9 2,434,178 - Pf6N2E2_2043 0.53 +0.8 2,434,178 - Pf6N2E2_2043 0.53 -2.0 2,434,178 - Pf6N2E2_2043 0.53 -0.4 2,434,320 + Pf6N2E2_2043 0.65 +0.4 2,434,605 - Pf6N2E2_2043 0.89 +0.2 2,434,605 - Pf6N2E2_2043 0.89 -0.3 2,434,605 - Pf6N2E2_2043 0.89 +0.3 2,434,612 + Pf6N2E2_2043 0.89 +0.4 2,434,612 + Pf6N2E2_2043 0.89 -0.6 2,434,612 + Pf6N2E2_2043 0.89 +1.0 2,434,612 + Pf6N2E2_2043 0.89 +2.0 2,434,613 - Pf6N2E2_2043 0.89 +0.2 2,434,613 - Pf6N2E2_2043 0.89 +0.7 2,434,613 - Pf6N2E2_2043 0.89 +0.6 2,434,613 - Pf6N2E2_2043 0.89 +1.6 2,434,741 + -0.8 2,434,742 - -0.3 2,434,742 - +0.3 2,434,757 + -0.7 2,434,757 + +2.0 2,434,758 - +0.3 2,434,760 + +0.3 2,434,761 - -0.0 2,434,761 - -0.2 2,434,826 + +0.0 2,434,828 + +1.0 2,434,828 + +1.5 2,434,828 + -0.0 2,434,828 + -0.8 2,434,828 + +1.0 2,434,828 + -0.3 2,434,828 + +0.5 2,434,828 + -0.3 2,434,828 + +1.4 2,434,829 - +0.3 2,434,829 - -0.5 2,434,829 - +0.2 2,434,829 - +0.7 2,434,829 - +1.2 2,434,829 - +0.6 2,434,829 - -0.1 2,434,829 - +0.3 2,434,977 + -2.4 2,435,042 + -1.6 2,435,043 - +0.1 2,435,043 - +0.4 2,435,066 + Pf6N2E2_2044 0.10 -0.8 2,435,066 + Pf6N2E2_2044 0.10 +0.8 2,435,082 + Pf6N2E2_2044 0.11 +0.4 2,435,082 + Pf6N2E2_2044 0.11 -0.1 2,435,083 - Pf6N2E2_2044 0.11 +0.1 2,435,083 - Pf6N2E2_2044 0.11 +0.6 2,435,083 - Pf6N2E2_2044 0.11 +0.4 2,435,083 - Pf6N2E2_2044 0.11 -0.3 2,435,083 - Pf6N2E2_2044 0.11 +2.0 2,435,138 - Pf6N2E2_2044 0.13 +0.8 2,435,181 + Pf6N2E2_2044 0.15 +2.2 2,435,181 + Pf6N2E2_2044 0.15 +0.5 2,435,181 + Pf6N2E2_2044 0.15 +0.8 2,435,181 + Pf6N2E2_2044 0.15 -0.3 2,435,181 + Pf6N2E2_2044 0.15 +0.7 2,435,181 + Pf6N2E2_2044 0.15 +2.0 2,435,182 - Pf6N2E2_2044 0.15 +0.8 2,435,394 - Pf6N2E2_2044 0.23 -0.4 2,435,457 - Pf6N2E2_2044 0.26 +0.1 2,435,457 - Pf6N2E2_2044 0.26 +1.4 2,435,556 + Pf6N2E2_2044 0.30 +0.2 2,435,595 + Pf6N2E2_2044 0.31 +1.0 2,435,595 + Pf6N2E2_2044 0.31 -0.3 2,435,595 + Pf6N2E2_2044 0.31 +0.3 2,435,596 - Pf6N2E2_2044 0.31 +0.8 2,435,613 + Pf6N2E2_2044 0.32 -0.0 2,435,613 + Pf6N2E2_2044 0.32 -0.0 2,435,613 + Pf6N2E2_2044 0.32 -1.2 2,435,614 - Pf6N2E2_2044 0.32 +0.2
Or see this region's nucleotide sequence