Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1318

Experiment: nitrogen source Ammonium chloride 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1317 and Pf6N2E2_1318 are separated by 311 nucleotidesPf6N2E2_1318 and Pf6N2E2_1319 are separated by 182 nucleotidesPf6N2E2_1319 and Pf6N2E2_1320 are separated by 51 nucleotides Pf6N2E2_1317 - P-hydroxyphenylacetate hydroxylase C2:oxygenase component, at 1,550,723 to 1,551,892 _1317 Pf6N2E2_1318 - FIG00553873: hypothetical protein, at 1,552,204 to 1,553,502 _1318 Pf6N2E2_1319 - FIG074102: hypothetical protein, at 1,553,685 to 1,554,050 _1319 Pf6N2E2_1320 - Putative response regulator, at 1,554,102 to 1,554,761 _1320 Position (kb) 1552 1553 1554Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1551.411 kb on + strand, within Pf6N2E2_1317at 1551.735 kb on + strand, within Pf6N2E2_1317at 1551.906 kb on + strandat 1551.907 kb on - strandat 1552.131 kb on - strandat 1552.133 kb on + strandat 1552.228 kb on + strandat 1552.228 kb on + strandat 1552.229 kb on - strandat 1552.229 kb on - strandat 1552.229 kb on - strandat 1552.231 kb on + strandat 1552.249 kb on + strandat 1552.249 kb on + strandat 1552.428 kb on + strand, within Pf6N2E2_1318at 1552.549 kb on + strand, within Pf6N2E2_1318at 1552.585 kb on + strand, within Pf6N2E2_1318at 1552.586 kb on - strand, within Pf6N2E2_1318at 1552.653 kb on + strand, within Pf6N2E2_1318at 1552.717 kb on + strand, within Pf6N2E2_1318at 1552.969 kb on + strand, within Pf6N2E2_1318at 1553.041 kb on + strand, within Pf6N2E2_1318at 1553.041 kb on + strand, within Pf6N2E2_1318at 1553.042 kb on - strand, within Pf6N2E2_1318at 1553.042 kb on - strand, within Pf6N2E2_1318at 1553.069 kb on - strand, within Pf6N2E2_1318at 1553.069 kb on - strand, within Pf6N2E2_1318at 1553.519 kb on + strandat 1553.519 kb on + strandat 1553.519 kb on + strandat 1553.519 kb on + strandat 1553.519 kb on + strandat 1553.520 kb on - strandat 1553.520 kb on - strandat 1553.520 kb on - strandat 1553.520 kb on - strandat 1553.520 kb on - strandat 1553.520 kb on - strandat 1553.839 kb on - strand, within Pf6N2E2_1319at 1553.874 kb on + strand, within Pf6N2E2_1319at 1553.874 kb on + strand, within Pf6N2E2_1319at 1553.874 kb on + strand, within Pf6N2E2_1319at 1553.952 kb on + strand, within Pf6N2E2_1319at 1554.033 kb on + strandat 1554.033 kb on + strandat 1554.153 kb on - strandat 1554.187 kb on - strand, within Pf6N2E2_1320at 1554.187 kb on - strand, within Pf6N2E2_1320at 1554.258 kb on + strand, within Pf6N2E2_1320at 1554.258 kb on + strand, within Pf6N2E2_1320at 1554.271 kb on - strand, within Pf6N2E2_1320at 1554.379 kb on - strand, within Pf6N2E2_1320

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM
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1,551,411 + Pf6N2E2_1317 0.59 +1.8
1,551,735 + Pf6N2E2_1317 0.86 -1.2
1,551,906 + -0.5
1,551,907 - +1.6
1,552,131 - +1.9
1,552,133 + -2.1
1,552,228 + +0.9
1,552,228 + -0.6
1,552,229 - +0.2
1,552,229 - +1.9
1,552,229 - -1.4
1,552,231 + +1.9
1,552,249 + +0.9
1,552,249 + -0.1
1,552,428 + Pf6N2E2_1318 0.17 -0.7
1,552,549 + Pf6N2E2_1318 0.27 -0.5
1,552,585 + Pf6N2E2_1318 0.29 -0.2
1,552,586 - Pf6N2E2_1318 0.29 +1.9
1,552,653 + Pf6N2E2_1318 0.35 +0.3
1,552,717 + Pf6N2E2_1318 0.39 +0.1
1,552,969 + Pf6N2E2_1318 0.59 +0.9
1,553,041 + Pf6N2E2_1318 0.64 -1.1
1,553,041 + Pf6N2E2_1318 0.64 -1.1
1,553,042 - Pf6N2E2_1318 0.65 -2.2
1,553,042 - Pf6N2E2_1318 0.65 +0.5
1,553,069 - Pf6N2E2_1318 0.67 +0.3
1,553,069 - Pf6N2E2_1318 0.67 -2.6
1,553,519 + -0.3
1,553,519 + -0.3
1,553,519 + +0.4
1,553,519 + -0.7
1,553,519 + +0.2
1,553,520 - -1.1
1,553,520 - -1.3
1,553,520 - -1.4
1,553,520 - +0.9
1,553,520 - -0.3
1,553,520 - +0.3
1,553,839 - Pf6N2E2_1319 0.42 +0.1
1,553,874 + Pf6N2E2_1319 0.52 +0.1
1,553,874 + Pf6N2E2_1319 0.52 -0.1
1,553,874 + Pf6N2E2_1319 0.52 +0.1
1,553,952 + Pf6N2E2_1319 0.73 -1.2
1,554,033 + +1.7
1,554,033 + -0.5
1,554,153 - +1.9
1,554,187 - Pf6N2E2_1320 0.13 -1.0
1,554,187 - Pf6N2E2_1320 0.13 +1.9
1,554,258 + Pf6N2E2_1320 0.24 -0.7
1,554,258 + Pf6N2E2_1320 0.24 -2.9
1,554,271 - Pf6N2E2_1320 0.26 -2.1
1,554,379 - Pf6N2E2_1320 0.42 -1.3

Or see this region's nucleotide sequence