Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1317 and Pf6N2E2_1318 are separated by 311 nucleotides Pf6N2E2_1318 and Pf6N2E2_1319 are separated by 182 nucleotides Pf6N2E2_1319 and Pf6N2E2_1320 are separated by 51 nucleotides
Pf6N2E2_1317 - P-hydroxyphenylacetate hydroxylase C2:oxygenase component, at 1,550,723 to 1,551,892
_1317
Pf6N2E2_1318 - FIG00553873: hypothetical protein, at 1,552,204 to 1,553,502
_1318
Pf6N2E2_1319 - FIG074102: hypothetical protein, at 1,553,685 to 1,554,050
_1319
Pf6N2E2_1320 - Putative response regulator, at 1,554,102 to 1,554,761
_1320
Position (kb)
1552
1553
1554 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1551.411 kb on + strand, within Pf6N2E2_1317 at 1551.735 kb on + strand, within Pf6N2E2_1317 at 1551.906 kb on + strand at 1551.907 kb on - strand at 1552.131 kb on - strand at 1552.133 kb on + strand at 1552.228 kb on + strand at 1552.228 kb on + strand at 1552.229 kb on - strand at 1552.229 kb on - strand at 1552.229 kb on - strand at 1552.231 kb on + strand at 1552.249 kb on + strand at 1552.249 kb on + strand at 1552.428 kb on + strand, within Pf6N2E2_1318 at 1552.549 kb on + strand, within Pf6N2E2_1318 at 1552.585 kb on + strand, within Pf6N2E2_1318 at 1552.586 kb on - strand, within Pf6N2E2_1318 at 1552.653 kb on + strand, within Pf6N2E2_1318 at 1552.717 kb on + strand, within Pf6N2E2_1318 at 1552.969 kb on + strand, within Pf6N2E2_1318 at 1553.041 kb on + strand, within Pf6N2E2_1318 at 1553.041 kb on + strand, within Pf6N2E2_1318 at 1553.042 kb on - strand, within Pf6N2E2_1318 at 1553.042 kb on - strand, within Pf6N2E2_1318 at 1553.069 kb on - strand, within Pf6N2E2_1318 at 1553.069 kb on - strand, within Pf6N2E2_1318 at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.839 kb on - strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.952 kb on + strand, within Pf6N2E2_1319 at 1554.033 kb on + strand at 1554.033 kb on + strand at 1554.153 kb on - strand at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.271 kb on - strand, within Pf6N2E2_1320 at 1554.379 kb on - strand, within Pf6N2E2_1320
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 1,551,411 + Pf6N2E2_1317 0.59 +1.8 1,551,735 + Pf6N2E2_1317 0.86 -1.2 1,551,906 + -0.5 1,551,907 - +1.6 1,552,131 - +1.9 1,552,133 + -2.1 1,552,228 + +0.9 1,552,228 + -0.6 1,552,229 - +0.2 1,552,229 - +1.9 1,552,229 - -1.4 1,552,231 + +1.9 1,552,249 + +0.9 1,552,249 + -0.1 1,552,428 + Pf6N2E2_1318 0.17 -0.7 1,552,549 + Pf6N2E2_1318 0.27 -0.5 1,552,585 + Pf6N2E2_1318 0.29 -0.2 1,552,586 - Pf6N2E2_1318 0.29 +1.9 1,552,653 + Pf6N2E2_1318 0.35 +0.3 1,552,717 + Pf6N2E2_1318 0.39 +0.1 1,552,969 + Pf6N2E2_1318 0.59 +0.9 1,553,041 + Pf6N2E2_1318 0.64 -1.1 1,553,041 + Pf6N2E2_1318 0.64 -1.1 1,553,042 - Pf6N2E2_1318 0.65 -2.2 1,553,042 - Pf6N2E2_1318 0.65 +0.5 1,553,069 - Pf6N2E2_1318 0.67 +0.3 1,553,069 - Pf6N2E2_1318 0.67 -2.6 1,553,519 + -0.3 1,553,519 + -0.3 1,553,519 + +0.4 1,553,519 + -0.7 1,553,519 + +0.2 1,553,520 - -1.1 1,553,520 - -1.3 1,553,520 - -1.4 1,553,520 - +0.9 1,553,520 - -0.3 1,553,520 - +0.3 1,553,839 - Pf6N2E2_1319 0.42 +0.1 1,553,874 + Pf6N2E2_1319 0.52 +0.1 1,553,874 + Pf6N2E2_1319 0.52 -0.1 1,553,874 + Pf6N2E2_1319 0.52 +0.1 1,553,952 + Pf6N2E2_1319 0.73 -1.2 1,554,033 + +1.7 1,554,033 + -0.5 1,554,153 - +1.9 1,554,187 - Pf6N2E2_1320 0.13 -1.0 1,554,187 - Pf6N2E2_1320 0.13 +1.9 1,554,258 + Pf6N2E2_1320 0.24 -0.7 1,554,258 + Pf6N2E2_1320 0.24 -2.9 1,554,271 - Pf6N2E2_1320 0.26 -2.1 1,554,379 - Pf6N2E2_1320 0.42 -1.3
Or see this region's nucleotide sequence