Experiment: DAC23_cps8_10MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0428 and BT0429 are separated by 11 nucleotides BT0429 and BT0430 are separated by 3 nucleotides BT0430 and BT0431 are separated by 80 nucleotides
BT0428: BT0428 - phenylacetate-coenzyme A ligase (NCBI ptt file), at 514,550 to 515,857
BT0428
BT0429: BT0429 - indolepyruvate oxidoreductase subunit iorB (NCBI ptt file), at 515,869 to 516,453
BT0429
BT0430: BT0430 - indolepyruvate oxidoreductase subunit iorA (NCBI ptt file), at 516,457 to 518,049
BT0430
BT0431: BT0431 - conserved hypothetical protein with conserved domain (NCBI ptt file), at 518,130 to 519,167
BT0431
Position (kb) 516
517
518
519 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 515.558 kb on - strand, within BT0428 at 515.594 kb on + strand, within BT0428 at 515.702 kb on - strand, within BT0428 at 515.702 kb on - strand, within BT0428 at 515.705 kb on - strand, within BT0428 at 515.746 kb on - strand at 515.866 kb on + strand at 515.867 kb on - strand at 515.870 kb on + strand at 515.917 kb on - strand at 516.091 kb on - strand, within BT0429 at 516.118 kb on + strand, within BT0429 at 516.207 kb on - strand, within BT0429 at 516.360 kb on - strand, within BT0429 at 516.391 kb on - strand, within BT0429 at 516.437 kb on - strand at 516.439 kb on - strand at 516.463 kb on - strand at 516.924 kb on + strand, within BT0430 at 516.935 kb on + strand, within BT0430 at 516.968 kb on + strand, within BT0430 at 516.969 kb on - strand, within BT0430 at 516.973 kb on - strand, within BT0430 at 516.973 kb on - strand, within BT0430 at 517.087 kb on + strand, within BT0430 at 517.106 kb on - strand, within BT0430 at 517.151 kb on + strand, within BT0430 at 517.363 kb on + strand, within BT0430 at 517.367 kb on + strand, within BT0430 at 517.368 kb on - strand, within BT0430 at 517.612 kb on + strand, within BT0430 at 517.613 kb on - strand, within BT0430 at 517.613 kb on - strand, within BT0430 at 517.623 kb on - strand, within BT0430 at 517.676 kb on + strand, within BT0430 at 517.676 kb on + strand, within BT0430 at 517.804 kb on + strand, within BT0430 at 517.804 kb on + strand, within BT0430 at 517.849 kb on - strand, within BT0430 at 517.896 kb on + strand at 517.935 kb on + strand at 517.978 kb on + strand at 517.978 kb on + strand at 517.978 kb on + strand at 517.978 kb on + strand at 517.979 kb on - strand at 517.979 kb on - strand at 518.052 kb on - strand at 518.254 kb on + strand, within BT0431 at 518.275 kb on - strand, within BT0431 at 518.278 kb on - strand, within BT0431 at 518.339 kb on + strand, within BT0431 at 518.341 kb on + strand, within BT0431 at 518.341 kb on + strand, within BT0431 at 518.342 kb on - strand, within BT0431 at 518.342 kb on - strand, within BT0431 at 518.445 kb on - strand, within BT0431 at 518.919 kb on - strand, within BT0431 at 519.030 kb on + strand, within BT0431 at 519.037 kb on - strand, within BT0431 at 519.037 kb on - strand, within BT0431 at 519.037 kb on - strand, within BT0431
Per-strain Table
Position Strand Gene LocusTag Fraction DAC23_cps8_10MOI remove 515,558 - BT0428 0.77 +2.5 515,594 + BT0428 0.80 -1.1 515,702 - BT0428 0.88 -1.4 515,702 - BT0428 0.88 -2.7 515,705 - BT0428 0.88 -2.0 515,746 - -1.9 515,866 + -2.1 515,867 - -3.0 515,870 + -0.7 515,917 - -0.7 516,091 - BT0429 0.38 -1.1 516,118 + BT0429 0.43 -0.1 516,207 - BT0429 0.58 +0.3 516,360 - BT0429 0.84 -0.7 516,391 - BT0429 0.89 -0.1 516,437 - +0.1 516,439 - -1.9 516,463 - -0.1 516,924 + BT0430 0.29 -0.1 516,935 + BT0430 0.30 -0.6 516,968 + BT0430 0.32 -2.1 516,969 - BT0430 0.32 +2.7 516,973 - BT0430 0.32 -1.8 516,973 - BT0430 0.32 -1.9 517,087 + BT0430 0.40 +1.9 517,106 - BT0430 0.41 -2.8 517,151 + BT0430 0.44 -0.3 517,363 + BT0430 0.57 +2.5 517,367 + BT0430 0.57 +0.9 517,368 - BT0430 0.57 +0.9 517,612 + BT0430 0.73 -1.4 517,613 - BT0430 0.73 +0.3 517,613 - BT0430 0.73 -3.8 517,623 - BT0430 0.73 +0.2 517,676 + BT0430 0.77 -0.4 517,676 + BT0430 0.77 -2.2 517,804 + BT0430 0.85 -0.5 517,804 + BT0430 0.85 +0.9 517,849 - BT0430 0.87 +0.4 517,896 + -2.5 517,935 + -1.9 517,978 + +0.9 517,978 + +1.2 517,978 + -1.2 517,978 + +0.3 517,979 - +0.9 517,979 - -0.7 518,052 - -0.3 518,254 + BT0431 0.12 -1.9 518,275 - BT0431 0.14 +0.5 518,278 - BT0431 0.14 -1.1 518,339 + BT0431 0.20 -1.4 518,341 + BT0431 0.20 -1.4 518,341 + BT0431 0.20 -0.1 518,342 - BT0431 0.20 +0.3 518,342 - BT0431 0.20 +1.9 518,445 - BT0431 0.30 -1.7 518,919 - BT0431 0.76 +0.9 519,030 + BT0431 0.87 -1.4 519,037 - BT0431 0.87 +0.9 519,037 - BT0431 0.87 +0.9 519,037 - BT0431 0.87 -0.8
Or see this region's nucleotide sequence