Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS04165

Experiment: D-Trehalose 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS04160 and PFLU_RS04165 are separated by 78 nucleotidesPFLU_RS04165 and PFLU_RS04170 are separated by 144 nucleotides PFLU_RS04160: PFLU_RS04160 - tryptophan--tRNA ligase, at 943,684 to 945,039 _RS04160 PFLU_RS04165: PFLU_RS04165 - cell division protein ZapE, at 945,118 to 946,212 _RS04165 PFLU_RS04170: PFLU_RS04170 - GlxA family transcriptional regulator, at 946,357 to 947,352 _RS04170 Position (kb) 945 946 947Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 945.079 kb on + strandat 945.079 kb on + strandat 945.117 kb on + strandat 945.117 kb on + strandat 945.118 kb on - strandat 945.118 kb on - strandat 945.118 kb on - strandat 945.118 kb on - strandat 945.128 kb on + strandat 945.129 kb on - strandat 945.129 kb on - strandat 945.136 kb on + strandat 945.137 kb on - strandat 945.137 kb on - strandat 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.304 kb on + strand, within PFLU_RS04165at 945.305 kb on - strand, within PFLU_RS04165at 945.305 kb on - strand, within PFLU_RS04165at 945.337 kb on + strand, within PFLU_RS04165at 945.337 kb on + strand, within PFLU_RS04165at 945.337 kb on + strand, within PFLU_RS04165at 945.337 kb on + strand, within PFLU_RS04165at 945.337 kb on + strand, within PFLU_RS04165at 945.338 kb on - strand, within PFLU_RS04165at 945.338 kb on - strand, within PFLU_RS04165at 945.338 kb on - strand, within PFLU_RS04165at 945.338 kb on - strand, within PFLU_RS04165at 945.338 kb on - strand, within PFLU_RS04165at 945.625 kb on + strand, within PFLU_RS04165at 945.625 kb on + strand, within PFLU_RS04165at 945.625 kb on + strand, within PFLU_RS04165at 945.625 kb on + strand, within PFLU_RS04165at 945.625 kb on + strand, within PFLU_RS04165at 945.625 kb on + strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.626 kb on - strand, within PFLU_RS04165at 945.949 kb on + strand, within PFLU_RS04165at 945.949 kb on + strand, within PFLU_RS04165at 945.949 kb on + strand, within PFLU_RS04165at 945.949 kb on + strand, within PFLU_RS04165at 945.950 kb on - strand, within PFLU_RS04165at 945.950 kb on - strand, within PFLU_RS04165at 945.950 kb on - strand, within PFLU_RS04165at 945.950 kb on - strand, within PFLU_RS04165at 945.950 kb on - strand, within PFLU_RS04165at 945.950 kb on - strand, within PFLU_RS04165at 945.950 kb on - strand, within PFLU_RS04165at 946.060 kb on + strand, within PFLU_RS04165at 946.060 kb on + strand, within PFLU_RS04165at 946.060 kb on + strand, within PFLU_RS04165at 946.061 kb on - strand, within PFLU_RS04165at 946.061 kb on - strand, within PFLU_RS04165at 946.061 kb on - strand, within PFLU_RS04165at 946.061 kb on - strand, within PFLU_RS04165at 946.061 kb on - strand, within PFLU_RS04165at 946.069 kb on - strand, within PFLU_RS04165at 946.069 kb on - strand, within PFLU_RS04165at 946.115 kb on - strandat 946.115 kb on - strandat 946.115 kb on - strandat 946.115 kb on - strandat 946.115 kb on - strandat 946.115 kb on - strandat 946.115 kb on - strandat 946.210 kb on + strandat 946.210 kb on + strandat 946.210 kb on + strandat 946.210 kb on + strandat 946.211 kb on - strandat 946.353 kb on + strandat 946.353 kb on + strandat 946.353 kb on + strandat 946.353 kb on + strandat 946.354 kb on - strandat 946.354 kb on - strandat 946.354 kb on - strandat 946.354 kb on - strandat 946.354 kb on - strandat 946.448 kb on + strandat 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.460 kb on + strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.461 kb on - strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.535 kb on + strand, within PFLU_RS04170at 946.536 kb on - strand, within PFLU_RS04170at 946.536 kb on - strand, within PFLU_RS04170at 946.536 kb on - strand, within PFLU_RS04170at 946.536 kb on - strand, within PFLU_RS04170at 946.536 kb on - strand, within PFLU_RS04170at 946.536 kb on - strand, within PFLU_RS04170at 946.548 kb on + strand, within PFLU_RS04170at 946.548 kb on + strand, within PFLU_RS04170at 946.548 kb on + strand, within PFLU_RS04170at 946.548 kb on + strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.549 kb on - strand, within PFLU_RS04170at 946.727 kb on + strand, within PFLU_RS04170at 946.727 kb on + strand, within PFLU_RS04170at 946.727 kb on + strand, within PFLU_RS04170at 946.727 kb on + strand, within PFLU_RS04170at 946.727 kb on + strand, within PFLU_RS04170at 946.727 kb on + strand, within PFLU_RS04170at 946.728 kb on - strand, within PFLU_RS04170at 946.728 kb on - strand, within PFLU_RS04170at 946.728 kb on - strand, within PFLU_RS04170at 946.728 kb on - strand, within PFLU_RS04170at 946.728 kb on - strand, within PFLU_RS04170at 946.795 kb on + strand, within PFLU_RS04170at 946.805 kb on + strand, within PFLU_RS04170at 946.805 kb on + strand, within PFLU_RS04170at 946.805 kb on + strand, within PFLU_RS04170at 946.805 kb on + strand, within PFLU_RS04170at 946.805 kb on + strand, within PFLU_RS04170at 946.806 kb on - strand, within PFLU_RS04170at 946.806 kb on - strand, within PFLU_RS04170at 946.806 kb on - strand, within PFLU_RS04170at 946.806 kb on - strand, within PFLU_RS04170at 946.806 kb on - strand, within PFLU_RS04170at 946.806 kb on - strand, within PFLU_RS04170at 946.806 kb on - strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.811 kb on + strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.812 kb on - strand, within PFLU_RS04170at 946.847 kb on + strand, within PFLU_RS04170at 946.847 kb on + strand, within PFLU_RS04170at 946.847 kb on + strand, within PFLU_RS04170at 946.848 kb on - strand, within PFLU_RS04170at 946.848 kb on - strand, within PFLU_RS04170at 946.848 kb on - strand, within PFLU_RS04170at 946.934 kb on + strand, within PFLU_RS04170at 946.934 kb on + strand, within PFLU_RS04170at 946.934 kb on + strand, within PFLU_RS04170at 946.934 kb on + strand, within PFLU_RS04170at 946.934 kb on + strand, within PFLU_RS04170at 946.934 kb on + strand, within PFLU_RS04170at 946.934 kb on + strand, within PFLU_RS04170at 946.935 kb on - strand, within PFLU_RS04170at 946.935 kb on - strand, within PFLU_RS04170at 947.005 kb on + strand, within PFLU_RS04170at 947.006 kb on - strand, within PFLU_RS04170at 947.006 kb on - strand, within PFLU_RS04170

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Trehalose 5 mM (C)
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945,079 + +0.5
945,079 + +0.7
945,117 + +0.3
945,117 + -0.7
945,118 - +1.0
945,118 - -0.4
945,118 - +0.8
945,118 - +0.7
945,128 + +0.1
945,129 - +2.2
945,129 - +0.5
945,136 + +1.5
945,137 - -0.9
945,137 - -0.9
945,304 + PFLU_RS04165 0.17 -1.0
945,304 + PFLU_RS04165 0.17 -0.5
945,304 + PFLU_RS04165 0.17 +1.5
945,304 + PFLU_RS04165 0.17 +1.8
945,304 + PFLU_RS04165 0.17 -0.5
945,304 + PFLU_RS04165 0.17 +2.5
945,304 + PFLU_RS04165 0.17 +1.0
945,304 + PFLU_RS04165 0.17 +1.5
945,304 + PFLU_RS04165 0.17 -0.2
945,304 + PFLU_RS04165 0.17 +1.5
945,304 + PFLU_RS04165 0.17 +0.1
945,305 - PFLU_RS04165 0.17 -0.5
945,305 - PFLU_RS04165 0.17 -0.3
945,337 + PFLU_RS04165 0.20 +1.5
945,337 + PFLU_RS04165 0.20 +2.8
945,337 + PFLU_RS04165 0.20 +1.7
945,337 + PFLU_RS04165 0.20 -1.4
945,337 + PFLU_RS04165 0.20 +0.3
945,338 - PFLU_RS04165 0.20 -1.1
945,338 - PFLU_RS04165 0.20 -0.5
945,338 - PFLU_RS04165 0.20 +0.5
945,338 - PFLU_RS04165 0.20 -0.1
945,338 - PFLU_RS04165 0.20 +0.1
945,625 + PFLU_RS04165 0.46 +1.5
945,625 + PFLU_RS04165 0.46 -1.1
945,625 + PFLU_RS04165 0.46 +2.5
945,625 + PFLU_RS04165 0.46 +2.0
945,625 + PFLU_RS04165 0.46 +0.6
945,625 + PFLU_RS04165 0.46 +0.5
945,626 - PFLU_RS04165 0.46 +2.5
945,626 - PFLU_RS04165 0.46 -0.9
945,626 - PFLU_RS04165 0.46 +1.0
945,626 - PFLU_RS04165 0.46 -0.1
945,626 - PFLU_RS04165 0.46 -0.5
945,626 - PFLU_RS04165 0.46 -0.1
945,626 - PFLU_RS04165 0.46 -0.1
945,626 - PFLU_RS04165 0.46 -0.1
945,949 + PFLU_RS04165 0.76 -1.1
945,949 + PFLU_RS04165 0.76 +0.1
945,949 + PFLU_RS04165 0.76 +0.3
945,949 + PFLU_RS04165 0.76 +2.5
945,950 - PFLU_RS04165 0.76 +2.1
945,950 - PFLU_RS04165 0.76 +0.3
945,950 - PFLU_RS04165 0.76 +1.5
945,950 - PFLU_RS04165 0.76 +1.3
945,950 - PFLU_RS04165 0.76 +0.5
945,950 - PFLU_RS04165 0.76 -0.4
945,950 - PFLU_RS04165 0.76 +1.5
946,060 + PFLU_RS04165 0.86 -1.9
946,060 + PFLU_RS04165 0.86 +2.5
946,060 + PFLU_RS04165 0.86 +2.1
946,061 - PFLU_RS04165 0.86 +0.9
946,061 - PFLU_RS04165 0.86 +0.9
946,061 - PFLU_RS04165 0.86 -1.1
946,061 - PFLU_RS04165 0.86 -0.0
946,061 - PFLU_RS04165 0.86 +0.9
946,069 - PFLU_RS04165 0.87 +0.9
946,069 - PFLU_RS04165 0.87 +2.5
946,115 - +0.5
946,115 - +1.5
946,115 - +1.0
946,115 - +2.9
946,115 - +1.5
946,115 - -0.1
946,115 - +0.7
946,210 + -2.9
946,210 + -0.4
946,210 + +0.6
946,210 + -0.9
946,211 - -0.5
946,353 + -1.5
946,353 + +1.5
946,353 + -0.1
946,353 + +0.9
946,354 - -0.1
946,354 - -0.8
946,354 - -1.4
946,354 - +0.4
946,354 - +0.2
946,448 + +0.4
946,460 + PFLU_RS04170 0.10 +0.5
946,460 + PFLU_RS04170 0.10 +0.5
946,460 + PFLU_RS04170 0.10 -0.9
946,460 + PFLU_RS04170 0.10 -1.2
946,460 + PFLU_RS04170 0.10 +0.0
946,460 + PFLU_RS04170 0.10 -0.1
946,460 + PFLU_RS04170 0.10 -0.1
946,460 + PFLU_RS04170 0.10 -1.9
946,460 + PFLU_RS04170 0.10 +0.1
946,460 + PFLU_RS04170 0.10 +0.3
946,461 - PFLU_RS04170 0.10 +0.6
946,461 - PFLU_RS04170 0.10 -0.1
946,461 - PFLU_RS04170 0.10 +1.3
946,461 - PFLU_RS04170 0.10 +0.4
946,461 - PFLU_RS04170 0.10 -1.1
946,461 - PFLU_RS04170 0.10 +0.3
946,461 - PFLU_RS04170 0.10 -0.2
946,461 - PFLU_RS04170 0.10 -0.1
946,461 - PFLU_RS04170 0.10 -0.6
946,461 - PFLU_RS04170 0.10 +1.6
946,461 - PFLU_RS04170 0.10 +0.3
946,535 + PFLU_RS04170 0.18 -1.2
946,535 + PFLU_RS04170 0.18 +0.7
946,535 + PFLU_RS04170 0.18 -0.4
946,535 + PFLU_RS04170 0.18 -2.0
946,535 + PFLU_RS04170 0.18 -0.7
946,535 + PFLU_RS04170 0.18 -0.8
946,535 + PFLU_RS04170 0.18 -3.1
946,535 + PFLU_RS04170 0.18 +0.3
946,536 - PFLU_RS04170 0.18 -0.3
946,536 - PFLU_RS04170 0.18 -0.7
946,536 - PFLU_RS04170 0.18 +0.3
946,536 - PFLU_RS04170 0.18 -0.1
946,536 - PFLU_RS04170 0.18 -3.0
946,536 - PFLU_RS04170 0.18 -0.3
946,548 + PFLU_RS04170 0.19 +0.4
946,548 + PFLU_RS04170 0.19 +0.1
946,548 + PFLU_RS04170 0.19 -0.0
946,548 + PFLU_RS04170 0.19 -0.1
946,549 - PFLU_RS04170 0.19 -0.7
946,549 - PFLU_RS04170 0.19 -0.4
946,549 - PFLU_RS04170 0.19 -1.5
946,549 - PFLU_RS04170 0.19 -0.5
946,549 - PFLU_RS04170 0.19 +0.9
946,549 - PFLU_RS04170 0.19 -0.9
946,549 - PFLU_RS04170 0.19 +0.0
946,549 - PFLU_RS04170 0.19 -0.9
946,727 + PFLU_RS04170 0.37 +0.3
946,727 + PFLU_RS04170 0.37 -0.2
946,727 + PFLU_RS04170 0.37 +1.3
946,727 + PFLU_RS04170 0.37 +0.3
946,727 + PFLU_RS04170 0.37 +0.8
946,727 + PFLU_RS04170 0.37 -0.7
946,728 - PFLU_RS04170 0.37 +0.1
946,728 - PFLU_RS04170 0.37 -0.4
946,728 - PFLU_RS04170 0.37 -1.1
946,728 - PFLU_RS04170 0.37 -0.7
946,728 - PFLU_RS04170 0.37 -0.2
946,795 + PFLU_RS04170 0.44 -1.1
946,805 + PFLU_RS04170 0.45 +0.2
946,805 + PFLU_RS04170 0.45 -0.3
946,805 + PFLU_RS04170 0.45 -0.4
946,805 + PFLU_RS04170 0.45 +0.2
946,805 + PFLU_RS04170 0.45 +0.8
946,806 - PFLU_RS04170 0.45 -0.7
946,806 - PFLU_RS04170 0.45 +1.3
946,806 - PFLU_RS04170 0.45 -0.0
946,806 - PFLU_RS04170 0.45 -0.9
946,806 - PFLU_RS04170 0.45 -0.1
946,806 - PFLU_RS04170 0.45 +0.7
946,806 - PFLU_RS04170 0.45 +0.0
946,811 + PFLU_RS04170 0.46 +0.0
946,811 + PFLU_RS04170 0.46 -0.2
946,811 + PFLU_RS04170 0.46 -1.2
946,811 + PFLU_RS04170 0.46 +0.4
946,811 + PFLU_RS04170 0.46 +1.3
946,811 + PFLU_RS04170 0.46 -0.0
946,811 + PFLU_RS04170 0.46 -0.2
946,811 + PFLU_RS04170 0.46 -0.5
946,811 + PFLU_RS04170 0.46 -0.5
946,811 + PFLU_RS04170 0.46 -0.8
946,811 + PFLU_RS04170 0.46 -1.4
946,811 + PFLU_RS04170 0.46 -2.0
946,811 + PFLU_RS04170 0.46 +0.5
946,811 + PFLU_RS04170 0.46 -0.7
946,811 + PFLU_RS04170 0.46 -1.1
946,811 + PFLU_RS04170 0.46 -0.6
946,811 + PFLU_RS04170 0.46 -0.7
946,811 + PFLU_RS04170 0.46 -0.1
946,811 + PFLU_RS04170 0.46 +1.0
946,811 + PFLU_RS04170 0.46 +0.8
946,812 - PFLU_RS04170 0.46 +0.0
946,812 - PFLU_RS04170 0.46 +0.5
946,812 - PFLU_RS04170 0.46 -0.7
946,812 - PFLU_RS04170 0.46 -0.5
946,812 - PFLU_RS04170 0.46 -2.0
946,812 - PFLU_RS04170 0.46 +0.2
946,812 - PFLU_RS04170 0.46 -1.7
946,812 - PFLU_RS04170 0.46 +1.8
946,812 - PFLU_RS04170 0.46 +0.3
946,812 - PFLU_RS04170 0.46 -1.3
946,812 - PFLU_RS04170 0.46 +0.4
946,812 - PFLU_RS04170 0.46 -0.1
946,812 - PFLU_RS04170 0.46 -0.2
946,812 - PFLU_RS04170 0.46 -0.3
946,812 - PFLU_RS04170 0.46 -0.5
946,812 - PFLU_RS04170 0.46 -1.2
946,847 + PFLU_RS04170 0.49 -0.1
946,847 + PFLU_RS04170 0.49 +0.3
946,847 + PFLU_RS04170 0.49 -0.5
946,848 - PFLU_RS04170 0.49 +0.6
946,848 - PFLU_RS04170 0.49 -1.0
946,848 - PFLU_RS04170 0.49 +1.0
946,934 + PFLU_RS04170 0.58 -0.5
946,934 + PFLU_RS04170 0.58 -0.8
946,934 + PFLU_RS04170 0.58 +0.3
946,934 + PFLU_RS04170 0.58 -1.2
946,934 + PFLU_RS04170 0.58 +0.0
946,934 + PFLU_RS04170 0.58 -0.8
946,934 + PFLU_RS04170 0.58 -0.4
946,935 - PFLU_RS04170 0.58 -0.2
946,935 - PFLU_RS04170 0.58 +0.5
947,005 + PFLU_RS04170 0.65 -0.8
947,006 - PFLU_RS04170 0.65 -0.5
947,006 - PFLU_RS04170 0.65 +0.1

Or see this region's nucleotide sequence